AJPS  Vol.7 No.3 , March 2016
Chemical Composition of the Fatty Oil from Fructus Broussonetiae and Its Effects on Rat Plasma Lipids and Adipose Tissue
Abstract: Context: Broussonetia papyrifera (L.) Vent. (Moraceae), a traditional Chinese medicinal herb, has been extensively applied for many years to treat various diseases. Its fruits (Fructus Broussonetiae) have been commonly used as an important tonic for the treatment of age-related disorders with long history; recent research has proved that it contains 32% to 35% fixed oils. The fixed oil is composed mainly of unsaturated fatty acids, including linoleic acid, methyl palmitate, oleic acid and linoleic acid ester. Objective: To investigate the chemistry of the fatty oil from Fructus Broussonetiae (FOFB) and its effects on plasma lipids. Methods: The chemical composition of FOFB was examined and identified by GC-MS. Thirty male Wistar rats fed diet containing FOFB and cholesterol were studied for 28 days. The effect of dietary FOFB on plasma lipids and adipose tissue was tested. Results: Twelve compounds of FOFB were examined and identified, the major components of fatty oil, 8,11-octadecadienoic acid (83.75%), palmitic acid (10.22%), octadecadienoic acid (2.97%) and 9-octadecenoic acid (1.69%) were found. FOFB significantly exhibited the activities of decreasing the rat adipose tissue weight, triacylglycerol, total cholesterol, and low-density lipoprotein cholesterol (LDL-C) concentrations while the rat body weight remained unchanged. Discussion: FOFB contained a large amount of PUFA which had the effect on reducing plasma lipids and adipose.
Cite this paper: Pang, S. , Wang, G. , Jin, X. , Sun, A. , Lin, J. and Diao, Y. (2016) Chemical Composition of the Fatty Oil from Fructus Broussonetiae and Its Effects on Rat Plasma Lipids and Adipose Tissue. American Journal of Plant Sciences, 7, 446-452. doi: 10.4236/ajps.2016.73038.

[1]   Pharmacopoeia Commission of People’s Republic of China (2010) Pharmacopoeia of People’s Republic of China. Chinese Medical Science & Technology Press, Beijing, 315.

[2]   Matsuda, H., Cai, H., Kubo, M., Tosa, H. and Iinuma, M. (1995) Study on Anti-Cataract Drugs from Natural Sources. II. Effects of Buddlejae Flos on in Vitro Aldose Reductase Activity. Biological & Pharmaceutical Bulletin, 18, 463-466.

[3]   Li, Z.M., Chen, J.J. and Yan, M.H. (2010) Studies on Chemical Constituents of Cortex of Broussonetia papyrifera. Journal of Anhui Agriculture Science, 38, 7288-7290.

[4]   Pang, S.Q., Wang, G.Q., Huang, B.K., et al. (2007) Cytotoxic Activities of Total Alkaloids Isolated from Fructus Broussonetia in Vitro. Journal of Chinese Medicinal Materials, 30, 826-828.

[5]   Guo, M.X., Wang, M.L., Deng, H., et al. (2013) A Novel Anticancer Agent Broussoflavonol B Downregulates Estrogen Receptor (ER)-α36 Expression and Inhibits Growth of ER-Negative Breast Cancer MDA-MB-231 Cells. European Journal of Pharmacology, 714, 56-64.

[6]   Pang, S.Q., Wang, G.Q., Qin, L.P., Huang, B.K. and Zhang, Q.Y. (2006) Antioxidation Activities of Fructus Broussonetia Haematochrome in Vitro. Journal of Chinese Medicinal Materials, 29, 262-265.

[7]   Matsuo, T., Takeuchi, H., Suzuki, H., Suzuki, H. and Suzuki, M. (2002) Body Fat Accumulation Is Greater in Rats Fed a Beef Tallow Diet than in Rats Fed a Safflower or Soybean Oil Diet. Asia Pacific Journal of Clinical Nutrition, 11, 302-308.

[8]   Li, Y.H., Hu, L. and Wu, Z.Z. (2011) Fructus Broussonetae Extract Improves Cognitive Function and Endoplasmic Reticulum Stress in Alzheimer’s Disease Models. Neural Regeneration Research, 6, 2325-2331.

[9]   Huang, B.K., Qin, L.P., Zheng, H.C., et al. (2003) Analysis of Amino Acid and Fatty Acid of Fructus Broussonetiae. Acadamic Journal of Second Military Medical University, 24, 213-213,217,227.

[10]   Fukuda, H., Iritani, N., Sugimoto, T. and Ikeda, H. (1999) Transcriptional Regulation of Fatty Acid Synthase Gene by Insulin/Glucose, Polyunsaturated Fatty Acid and Leptin in Hepatocytes and Adipocytes in Normal and Genetically Obese Rats. European Journal of Biochemistry, 260, 505-511.

[11]   Yuan, X. and Yuan, P. (2005) Studies on the Effects of Oil and Flavanoids from Fructus Broussonetiae’s Antioxidation and Elimination of Free Radicals. Natural of Product Research Developing, 17, 23-26.

[12]   Zhao, H., Huang, L., Qin, L., et al. (2011) Antioxidative and Anti-Inflammatory Properties of Chushizi Oil from Fructus Broussonetiae. Journal of Medicinal Plants Research, 5, 6407-6412.

[13]   Folch, J., Less, M. and Sloane, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissue. Journal of Biological Chemistry, 226, 497-509.

[14]   Jiang, T., Wang, Z.W., Proctor, G., et al. (2005) Diet-Induced Obesity in C57BL/6J Mice Causes Increased Renal Lipid Accumulation and Glomerulosclerosis via a Sterol Regulatory Element-Binding Protein-1c-Dependent Pathway. Journal of Biological Chemistry, 280, 32317-32325.

[15]   Shimomura, Y., Tamura, T. and Suzuki, M. (1990) Less Body Fat Accumulation in Rats Fed a Safflower Oil Diet than in Rats Fed a Beef Tallow Diet. Journal of Nutrition, 120, 1291-1296.

[16]   Edwards, M.S., Smith, B.A., Kainer, R.A., et al. (1993) Effect of Dietary Fat and Aging on Adipose Tissue Cellularity in Mice Differing in Genetic Predisposition to Obesity. Growth Development of Aging, 57, 45-51.

[17]   Jones, B.H., Maher, M.A., Banz, W.J., et al. (1996) Adipose Tissue Stearoyl-CoA Desaturase mRNA Is Increased by Obesity and Decreased by Polyunsaturated Fatty Acids. American Journal of Physiology, 271, E44-E49.

[18]   Sessler, A.M. and Ntambi, J.M. (1998) Polyunsaturated Fatty Acid Regulation of Gene Expression. The Journal of Nutrition, 128, 923-926.

[19]   Luo, R. and Qu, S.Y. (2004) Obesity and Free Radicals. Section of Endocrinology Foreign Medical Sciences, 24, 9-10.

[20]   Chang, N.W. and Huang, P.C. (1990) Effects of Dietary Monounsaturated Fatty Acids on Plasma Lipids in Humans. Journal of Lipid Research, 3, 2141-2147.

[21]   Nigon, F., Serfaty-Lacrosnière, C., Beucler, I., et al. (2001) Plant Sterol-Enriched Margarine Lowers Plasma LDL in Hyperlipidemic Subjects with Low Cholesterol Intake: Effect of Fibrate Treatment. Clinical Chemistry and Laborotary Medicine, 39, 634-640.

[22]   Lee, S., Lee, Y.S., Jung, S.H., Kang, S.S. and Shin, K.H. (2003) Anti-Oxidant Activities of Fucosterol from the Marine Algae Pelvetia siliquosa. Archives of Pharmacal Research, 26, 719-722.