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 AJPS  Vol.7 No.1 , January 2016
Endophytic Fungi Associated with a Holoparasitic Plant, Balanophora japonica (Balanophoraceae)
Abstract: Holoparasitism is a special life cycle of flowering plants. All carbon resources are provided by photosynthetic host plants. A recent study revealed the presence of endophytic fungi in holoparasitic plants, but their ecological and evolutionary roles are still unknown. In this study, we examined endophytic fungi isolated from the holoparasitic plant Balanophora japonica (Balanophoraceae), collected from Kochi, Shikoku in western Japan. We isolated 23 fungal strains on inflorescences and tubers from three B. japonica plants at two locations and on one sample of the host plant (Symplocos lancifolia, Symplocaceae). Predominant isolates were Trichoderma-Hypocrea, Penicillium and Phialemonium. The first group was also predominant in the host plant. Fungal composition revealed in this study differed from the composition on B. harlandii or other root holoparasites with endophytic fungal (Rafflesia cantleyi) data. Those differences might be caused by various factors, including growth habits, location, phylogenetic position or host-parasite relationship.
Cite this paper: Ikeda, H. , Fukuda, T. , Yokoyama, J. (2016) Endophytic Fungi Associated with a Holoparasitic Plant, Balanophora japonica (Balanophoraceae). American Journal of Plant Sciences, 7, 152-158. doi: 10.4236/ajps.2016.71016.
References

[1]   Carroll, G. (1988) Fungal Endophytes in Stems and Leaves: From Latent Pathogen to Mutualistic Symbiont. Ecology, 69, 2-9.
http://dx.doi.org/10.2307/1943154

[2]   Schardl, C.L. and Phillips, T.D. (1997) Protective Grass Endophytes. Where Are They from and Where Are They Going? Plant Disease, 81, 430-438.
http://dx.doi.org/10.1094/PDIS.1997.81.5.430

[3]   Stone, J.K., Bacon, C.W. and White, J.F. (2000) An Overview of Endophytic Microbes: Endophytism Defined. In: Bacon, C.W. and White, J.F., Eds., Microbial Endophytes, Marcel Dekker, New York, 3-29.

[4]   Verma, A., Verma, S., Sudha, Sahay, N., Bütehorn, B. and Franken, P. (1999) Piriformospora indica, a Cultivable Plant-Growth-Promoting Root Endophyte. Applied and Environmental Microbiology, 65, 2741-2744.

[5]   Bae, H., Sicher, R.C., Kim, M.S., Kim, S.-H., Strem, M.D., Melnick, R.L. and Bailey, B.A. (2009) The Beneficial Endophyte Trichoderma hamatum Isolate DIS 219b Promotes Growth and Delays the Onset of the Drought Response in Theobroma cacao. Journal of Experimental Botany, 60, 3279-3295.
http://dx.doi.org/10.1093/jxb/erp165

[6]   Hermosa, R., Viterbo, A., Chet, I. and Monte, E. (2012) Plant-Beneficial Effects of Trichoderma and of Its Genes. Microbiology, 158, 17-25.
http://dx.doi.org/10.1099/mic.0.052274-0

[7]   Rodriguez, R.J., Redman, R.S. and Henson, J.M. (2004) The Role of Fungal Symbioses in the Adaptation of Plants to High Stress Environments. Mitigation and Adaptation Strategies for Global Change, 9, 261-272.
http://dx.doi.org/10.1023/B:MITI.0000029922.31110.97

[8]   Rodriguez, R.J. and Redman, R.S. (2008) More than 400 Million Years of Evolution and Some Plants Still Can’t Make It on Their Own: Plant Stress Tolerance via Fungal Symbiosis. Journal of Experimental Botany, 59, 1109-1114.
http://dx.doi.org/10.1093/jxb/erm342

[9]   Worchel, E.R., Giauque, H.E. and Kivlin, S.N. (2013) Fungal Symbionts Alter Plant Drought Response. Miclobial Ecology, 65, 671-678.
http://dx.doi.org/10.1007/s00248-012-0151-6

[10]   Arnold, A.E., Mejia, L.C., Kyllo, D., Rojas, E.I., Maynard, Z., Robbins, N. and Herre, E.A. (2003) Fungal Endophytes Limit Pathogen Damage in a Tropical Tree. Proceeding of the National Academy of Sciences of the United States of America, 100, 15649-15654.
http://dx.doi.org/10.1073/pnas.2533483100

[11]   Czarnoleski, M., Pawlik, K., Olejniczak, P., Kozlowski, J. and Lembicz, M. (2012) An Endophytic Fungus Reduces Herbivory in Its Recently Colonised Grass Host: A Food-Choice Experiment on Common Voles, Weeping Alkaligrass and Epichloë typhina. Plant Ecology, 213, 1049-1053.
http://dx.doi.org/10.1007/s11258-012-0064-y

[12]   Press, M.C. and Graves, J.D. (Eds.) (1995) Parasitic Plants. Chapman & Hall, London, 292.

[13]   Nickrent, D.L. and Musselman, L.J. (2004) Introduction to Parasitic Flowering Plants. The Plant Health Instruc-tor.
http://www.apsnet.org/edcenter/intropp/PathogenGroups/Pages/ParasiticPlants.aspx
http://dx.doi.org/10.1094/PHI-I-2004-0330-01


[14]   Refaei, J., Jones, E.B.G., Sakayaroj, J. and Santhanam, J. (2011) Endophytic Fungi from Rafflesia cantleyi: Species Diversity and Antimicrobial Activity. Mycosphere, 2, 429-447.

[15]   Suryanarayanan, T.S., Senthilarasu, G. and Muruganandam, V. (2000) Endophytic Fungi form Cuscuta reflexa and Its Host Plants. Fungal Diversity, 4, 117-123.

[16]   Stevens, P.F. (2001) Angiosperm Phylogeny Website. Version 12, July 2012 [and More or Less Continuously Updated Since].
http://www.mobot.org/MOBOT/research/APweb/

[17]   Su, H.J., Hu, J.M., Anderson, F.E., Der, J.P. and Nickrent, D.L. (2015) Phylogenetic Relationships of Santalales with Insights into the Origins of Holoparasitic Balanophoraceae. Taxon, 64, 491-506.
http://dx.doi.org/10.12705/643.2

[18]   Tu, X., Zhang, Y., Han, Q., Yu, C. and An, R. (2011) Isolation and Screening of Endophytic Antibacteria from Balanophora harlandii Hook.f. Chinese Agricultural Science Bulletin, 27, 309-312.

[19]   Hansen, B. (1972) The Genus Balanophora, a Taxonomic Monograph. Dansk Botanisk Arkiv, 28, 1-188.

[20]   Su, H.J., Murata, J. and Hu, J.M. (2012) Morphology and Phylogeny of Two Holoparasitic Plants, Balanophora japonica and Balanophora yakushimensis (Balanophoraceae), and Their Hosts in Taiwan and Japan. Journal of Plant Research, 125, 317-326.

[21]   White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis, M., Gelfand, D., Sninsky, J. and White, T.J., Eds., PCR Protocols: A Guide to Methods and Application, Academic Press, San Diego, 315-322.
http://dx.doi.org/10.1016/b978-0-12-372180-8.50042-1

[22]   Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs. Nucleic Acids Research, 25, 3389-3402.
http://dx.doi.org/10.1093/nar/25.17.3389

[23]   Hoff, J.A., Klopfenstein, N.B., McDonald, G.I., Tonn, J.R., Kim, M.-S., Zambino, P.J., Hessburg, P.F., Rogers, J.D., Peever, T.L. and Carris, L.M. (2004) Fungal Endophytes in Woody Roots of Douglasfir (Pseudotsuga menziesii) and Ponderosa Pine (Pinus ponderosa). Forest Pathology, 34, 255-271.
http://dx.doi.org/10.1111/j.1439-0329.2004.00367.x

[24]   Summerbell, R.C. (2005) Root Endophyte and Mycorrhizosphere Fungi of Black Spruce, Picea mariana, in a Boreal Forest Habitat: Influence of Site Factors on Fungal Distributions. Studies in Mycology, 53, 121-145.
http://dx.doi.org/10.3114/sim.53.1.121

[25]   Gazis, R. and Chaverri, P. (2010) Diversity of Fungal Endophytes in Leaves and Stems of Wild Rubber Trees (Hevea brasiliensis) in Peru. Fungal Ecology, 3, 240-254.
http://dx.doi.org/10.1016/j.funeco.2009.12.001

[26]   Suryanarayanan, T.S. and Vijaykrishna, D. (2001) Fungal Endophytes of Aerial Roots of Ficus benghalensis. Fungal Diversity, 8, 155-161.

[27]   Maheswari, S. and Rajagopal, K. (2011) Biodiveristy of Endophytic Fungi Associated with Ficus religiosa and F. benghalensis. Mycologia Balcanica, 8, 169-172.

[28]   Photita, W., Taylor, P.W.J., Ford, R., Hyde, K.D. and Lumyong, S. (2005) Morphological and Molecular Characterization of Colletotrichum Species from Herbaceous Plants in Thailand. Fungal Diversity, 18, 117-133.

[29]   Gonzaga, L.L., Costa, L.E.O., Santos, T.T., Araújo, E.F. and Queiroz, M.V. (2014) Endophyic Fungi from the Genus Colletotrichum Are Abundant in the Phaseolus vulgaris and Have High Genetic Diversity. Journal of Applied Microbiology, 118, 485-496.
http://dx.doi.org/10.1111/jam.12696

 
 
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