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 CM  Vol.6 No.4 , December 2015
Total Flavonoid Content, the Antioxidant Capacity, Fingerprinting and Quantitative Analysis of Fupenzi (Rubus chingii Hu.)
Abstract: Objective: In this study, one of the objectives was to investigate the total flavonoid contents of Fupenzi (R. chingii Hu.) obtained from different regions of China and to evaluate their anatioxidant activities. And the second objective of this study was to develop a validated HPLC method for chromatographic fingerprints of the samples extracts of Fupenzi. Method: The total flavonoid contents were determined by a colorimetric method and the antioxidant activity was determined spectrophotometrically by DPPH and ABTS radical scavenging assays. The chromatographic fingerprint was developed by high-performance liquid chromatography coupled with diode array detection for the control of Fupenzi. Results: A significant correlation between antioxidant activity and the total flavonoid content was observed for the DPPH assay (r2 = 0.758, ρ = 0.004) and the ABTS assay (r2 = 0.788, ρ = 0.002). Under the optimized chromatographic conditions, the validated method was successfully applied to assessment of chemical fingerprinting of 12 batches of FPZ collected from different regions of China. Comparisons of the chromatograms showed that 15 characteristic peaks could be selected as markers for identification and evaluation of Fupenzi. In addition, the proposed method was also successfully applied to simultaneous determination of five compounds (including puerarin, rutin, hyperin, quercetin and kaempferol) in these samples. Conclusions: The qualitative and quantitative analysis described in this paper could be used for identification and evaluation of Fupenzi.
Cite this paper: Zeng, H. , Yang, R. , Lei, L. and Wang, Y. (2015) Total Flavonoid Content, the Antioxidant Capacity, Fingerprinting and Quantitative Analysis of Fupenzi (Rubus chingii Hu.). Chinese Medicine, 6, 204-213. doi: 10.4236/cm.2015.64023.
References

[1]   National Pharmacopoeia Committee (2010) Pharmacopoeia of People’s Republic of China. Part 1. Chemical Industry Press, Beijing, 359.

[2]   Rommel, A. and Wrolstad, R.E. (1993) Influence of Acid and Base Hydrolysis on the Phenolic Composition of Red Raspberry Juice. Journal of Agricultural and Food Chemistry, 41, 1237-1241.
http://dx.doi.org/10.1021/jf00032a014

[3]   Hakkinen, S.H., Karenlampi, S.O., Heinonen, I.M., Mykkanen, H.M. and Torronen, A.R. (1998) HPLC Method for Screening of Flavonoids and Phenolic Acids in Berries. Journal of Agricultural and Food Chemistry, 77, 543-551.
http://dx.doi.org/10.1002/(SICI)1097-0010(199808)77:4<543::AID-JSFA78>3.0.CO;2-I

[4]   Dejaegher, B., Alaerts, G. and Matthijs, N. (2010) Methodology to Develop Liquid Chromatographic Fingerprints for the Quality Control of Herbal Medicines. Acta Chromatographica, 22, 237-258.
http://dx.doi.org/10.1556/AChrom.22.2010.2.7

[5]   Jiang, Y., David, B., Tu, P. and Barbin, Y. (2010) Recent Analytical Approaches in Quality Control of Traditional Chinese Medicines—A Review. Analytica Chimica Acta, 657, 9-18.
http://dx.doi.org/10.1016/j.aca.2009.10.024

[6]   Liang, Y., Xie, P. and Chan K. (2004) Quality Control of Herbal Medicines. Journal of Chromatography B, 812, 53-70.
http://dx.doi.org/10.1016/S1570-0232(04)00676-2

[7]   Gutierrez, J.R.L., Coello, J. and Maspoch, S. (2012) Enhanced Chromatographic Fingerprint of Herb Materials by Multi-Wavelength Selection and Chemometrics. Analytica Chimica Acta, 710, 40-49.
http://dx.doi.org/10.1016/j.aca.2011.10.010

[8]   Chou, G.X., Xu, S.J., Liu, D., Kou, G.Y., Zhang, J. and Liu, Z.J. (2009) Quantitative and Fingerprint Analyses of Chinese Sweet Tea Plant (Rubus suavissimus S. Lee). Journal of Agricultural and Food Chemistry, 57, 1076-1083.
http://dx.doi.org/10.1021/jf8029397

[9]   Chen, J., Lu, Y.H., Wei, D.Z. and Zhou, X.L. (2009) Establishment of a Fingerprint of Raspberries by LC. Chromatographia, 70, 981-985.
http://dx.doi.org/10.1365/s10337-009-1217-6

[10]   Meyers, K.J., Watkins, C.B., Pritts, M.P. and Liu, R.H. (2003) Antioxidant and Antiproliferative Activities of Strawberries. Journal of Agricultural and Food Chemistry, 51, 6887-6892.
http://dx.doi.org/10.1021/jf034506n

[11]   Chen, C.W. and Ho, C.T. (1995) Antioxidant Properties of Polyphenols Extracted from Green and Black Teas. Journal of Food Lipids, 2, 35-46.
http://dx.doi.org/10.1111/j.1745-4522.1995.tb00028.x

[12]   Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine, 26, 1231-1237.
http://dx.doi.org/10.1016/S0891-5849(98)00315-3

[13]   Tsantili, E., Konstantinidis, K., Christopoulos, M.V. and Roussos, P.A. (2011) Total Phenolics and Flavonoids and Total Antioxidant Capacity in Pistachio (Pistachia vera L.) Nuts in Relation to Cultivars and Storage Conditions. Scientia Horticulturae, 129, 694-701.
http://dx.doi.org/10.1016/j.scienta.2011.05.020

[14]   Venskutonis, P.R., Dvaranauskaite, A. and Labokas, J. (2007) Raidcal Scavenging Activity and Composition of Raspberry (Rubus idaeus) Leaves from Different Locations in Lithuania. Fitoterapia, 78, 162-165.
http://dx.doi.org/10.1016/j.fitote.2006.10.001

[15]   Anttonen, M.J. and Karjalainen, R.O. (2005) Environmental and Genetic Variation of Phenolic Compounds in Red Raspberry. Journal of Food Composition and Analysis, 18, 759-769.
http://dx.doi.org/10.1016/j.jfca.2004.11.003

[16]   Makris, D.P. and Rossiter, J.T. (2001) Comparison of Quercetin and a Non-Orthohydroxy Flavonol as Antioxidants by Competing in Vitro Oxidation Reactions. Journal of Agricultural and Food Chemistry, 49, 3370-3377.
http://dx.doi.org/10.1021/jf010107l

[17]   Knekt, P., Kumpulainen, J., Jarvinen, R., Rissanen, H., Heliovaara, M., Reunanen, A., Hakulinen, T. and Aromaa, A. (2002) Flavonoid Intake and Risk of Chronic Diseases. American Journal of Clinical Nutrition, 76, 560-568.

[18]   Hubbard, G.P., Stevens, J.M., Cicmil, M., Sage, T., Jordan, P.A., Williams, C.M., Lovegrove, J.A. and Gibbins, J.M. (2003) Quercetin Inhibits Collagen-Stimulated Platelet Activation through Inhibition of Multiple Components of the Glycoprotein VI Signaling Pathway. Journal of Thrombosis and Haemostasis, 1, 1079-1088.
http://dx.doi.org/10.1046/j.1538-7836.2003.00212.x

 
 
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