ABC  Vol.5 No.7 , December 2015
Somatolactin Transcription during Oogenesis in Female Blue Gourami (Trichogaster trichopterus)
Abstract: Somatolactin (SL), a specific pituitary hormone belonging to the prolactin (PRL) super family, is involved in background adaptation, osmoregulation, reproduction and fatty acid metabolism. The goal of this study was to examine the gene transcription of SL changes in the ovary of blue gourami females (Trichogaster trichopterus) during oogenesis by quantitative real-time polymerase chain reaction (Real-Time PCR). Somatolactin in the pituitary was higher in females at low vitellogenesis compared to females with oocytes in maturation, and the difference was significant (p < 0.05). No significant differences were found in mRNA levels between low and high vitellogenesis, and high vitellogenesis and maturation. The findings of this and previous studies demonstrate that SL, growth hormone (GH) and PRL are involved in oogenesis in blue gourami; however, considerably more studies are required in order to separate the functions of these hormones.
Cite this paper: Degani, G. (2015) Somatolactin Transcription during Oogenesis in Female Blue Gourami (Trichogaster trichopterus). Advances in Biological Chemistry, 5, 279-285. doi: 10.4236/abc.2015.57025.

[1]   Degani, G. (2001) Blue Gourami (Trichogaster trichopterus) Model for Labyrinth Fish. Laser Pages Publishing, Israel.

[2]   Degani, G. (1993) The Effect of Sexual Behaviour on Oocyte Development and Steroid Changes in Trichogaster trichopterus. Copeia, 4, 1091-1096.

[3]   Jackson, K., Degani, G. and Goldberg, D. (2004) Transcription of FSH-b, LH-b and Growth Hormone in Female Trichogaster trichopterus (Pallas 1770) Treated with GnRH Analog. IV International Congress on the Biology of Fish, Manaus, 45-57.

[4]   Becker, D., Galili, M. and Degani, G. (1992) GCNS-Identified Steroids and Steroids Goucoronides in Gonads and Holding Waterin Gonads and Holding Water of Trichogaster tricopterus (Anabantidea, Pallas 1770). Comparative Biochemistry and Physiology, 103B, 15-19.

[5]   Degani, G. and Schreibman, M.P. (1993) Pheromone of Male Blue Gourami and Its Effect on Vitellogenesis and Gonadotropin Cells in Ptuitary of Females. Journal of Fish Biology, 43, 475-485.

[6]   Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue Gourami (Trichogaster trichopterus) Gonadotropic Beta Subunits (I and II) cDNA Sequences and Expression during Oogenesis. Journal of Molecular Endocrinology, 23, 177-187.

[7]   Degani, G., Yom-Din, S., Goldberg, D. and Jackson, K. (2010) cDNA Cloning of Blue Gourami (Trichogaster trichopterus) Prolactin and Its Expression during the Gonadal Cycles of Males and Females. Journal of Endocrinological Investigation, 33, 7-12.

[8]   Degani, G. (2014) Expression of the Growth Hormone and Insulin-Like Growth Factor 1 Genes in the Male and Female Blue Gourami (Trichogaster trichopterus) at Different Temperatures. Journal of Asian Scientific Research, 4, 413-427.

[9]   Degani, G., Jackson, K. and Goldberg, G. (2000) Blue Gourami b GTH-I, b GTH-II and GH Gene Expression Affected by GnRH Analog. Comparative Biochemistry and Physiology—Part A, 126, 42.

[10]   Goldberg, D., Jackson, K., Yom-Din, S. and Degani, G. (2004) Growth Hormone of Trichogaster trichopterus: cDNA Cloning, Sequencing and Analysis of mRNA Expression during Oogenesis. Journal of Aquaculture in the Tropics, 19, 215-229.

[11]   Degani, G., Jackson, K., Yom-Din, S. and Goldberg, D. (2006) CDNA Cloning and mRNA Expression of Growth Hormone in Belontiidae (Anabantoidei Suborder). The Israeli Journal of Aquaculture, 58, 124-136.

[12]   Levy, G. and Degani, G. (2012) Involvement of GnRH, PACAP and PRP in the Reproduction of Blue Gourami Females (Trichogaster trichopterus). Journal of Molecular Neuroscience, 48, 603-616.

[13]   Levy, G. and Degani, G. (2011) Evidence of a Reproduction-Related Function for Pituitary Adenylate Cyclase-Activating Polypeptide-Related Peptide in an Anabantidae Fish. Journal of Mo-lecular Endocrinology, 46, 101-110.

[14]   Levy, G. and Degani, G. (2013) The Role of Brain Peptides in the Reproduction of Blue Gourami Males (Trichogaster trichopterus). Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 319, 461-470.

[15]   Perea, S.G. (2008) Growth Hormone and Somatolactin Function during Sexual Maturation of Female Atlantic Salmon. Department of Zoology/Zoophysiology University of Gothenburg, Sweden, 1-79.

[16]   Degani, G. (2014) Involvement of GnRH and Gonadotropin Genes in Oocyte Development of Blue Gourami Females (Trichogaster trichopterus). Advances in Biological Chemistry, 4, 197-202.

[17]   Jackson, K., Abraham, M. and Degani, G. (1994) Oocyte Maturation Triggered by the Presence of Male in the Blue Gourami, Trichogaster trichopterus. Journal of Morphology, 220, 1-9.

[18]   Degani, G., Gal, E. and Vaya, J. (1994) In Vitro Biosynthesis of Steroids in Ovary of Asynchronic Trichogaster trichopterus (Pallus, 1770). Comparative Biochemistry and Physiology, 109, 715-723.

[19]   Jackson, K., Abraham, M. and Degani, G. (2005) Cellular Events in Trichogaster trichopterus Adenohy-pophysis during Final Oocyte Maturation. Indian Journal of Fisheries, 52, 1-13.

[20]   Degani, G. (2013) Underground Water Affects Sexual Behavior and Gene Expression of Hormones Related to Reproduction in Blue Gourami Males. Advances in Biological Chemistry, 3, 133-140.

[21]   Pfaffl, M.W. (2001) A New Mathematical Model for Relative Quantification in Real-Time RT-PCR. Nucleic Acids Research, 29, e45.

[22]   Muller, P.Y., Janovjak, H., Miserez, A.R. and Dobbie, Z. (2002) Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR. Biotechniques, 32, 1372-1374, 1376, 1378-1379.

[23]   Levy, G., Gothilf, Y. and Degani, G. (2009) Brain Gonadotropin Releasing Hormone3 Expression Variation during Oogenesis and Sexual Behavior and Its Effect on Pituitary Hormonal Expression in the Blue Gourami. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 154, 241-248.

[24]   Bjornsson, B.T., Benedet, S., Taranger, G.L. and Andersson, E. (2008) Cloning of Somatolactin Alpha, Beta Forms and the Somatolactin Receptor in Atlantic Salmon: Aeasonal Expression Profile in Pituitary and Ovary of Maturing Female Broodstock. Reproductive Biology and Endocrinology, 6, 42.

[25]   Fukamachi, S., Yada, T. and Mitani, H. (2005) Medaka Receptors for Somatolactin and Growth Hormone: Phylogenetic Paradox among Fish Growth Hormone Receptors. Genetics, 171, 1875-1883.