Sabrina Moro Villela Pacheco¹, Américo Cruz Júnior¹, Ayres Ferreira Morgado¹, Agenor Furigo Júnior¹, Onyetugo Chioma Amadi², José Manuel Guisán³, Benevides Pessela^4,5*

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¹ Department of Food and Chemistry Engineering, University Campus UFSC, Florianópolis, Brazil.
² Department of Microbiology, Faculty of Biological Science, University of Nigeria, Enugu, Nigeria.
³ Departamento de Biocatálises Enzimática, Instituto de Catalisis y Petroleoquimica, ICP (CSIC-UAM), C/Marie Curie 2, Cantoblanco, Campus de la Univerisdad Autónoma de Madrid, Madrid, España.
⁴ Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), C/Nicolás Cabrera 9, Cantoblanco, Campus de la Universidad Autónoma de Madrid, Madrid, España.
⁵ Departamento de Engenharia e Tecnologías, Instituto Superior Politécnico de Tecnología e de Ciências, ISPTEC, Luanda Sul, Talatona, República de Angola.
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Abstract: Nineteen fungal strains were isolated from a chicken slaughterhouse effluent and within those, only one showed high values of lipolytic activity in submerged cultures. This fungus was identified as Trametes hirsuta. The crude extract was immobilized in chitosan/clay beads, with an immobilization yield of 80.9%. The analyses of the crude extract and the immobilized derivative at different temperatures, pH (s), solvents, metallic ions and storage showed that the immobilization process increased the enzyme life span. Ethyl esters were obtained in solvent free systems using chicken viscera oil and the enzyme crude extract. For effective comparison, a reaction using viscera oil and commercial lipase Novozym 435 was carried out. The result revealed 35% and 28% esters conversion in the reactions containing chicken viscera oil, using Novozym 435 and the crude extract respectively. The extract was also used in a reaction with soybean oil, traditionally used as starting substrate for biodiesel production.

Keywords: Biodiesel Production, Trametes Hirsuta, Lipase

Cite this paper: Pacheco, S. , Júnior, A. , Morgado, A. , Júnior, A. , Amadi, O. , Guisán, J. , Pessela, B. (2015) Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production. Advances in Enzyme Research, 3, 101-114. doi: 10.4236/aer.2015.34011.

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