WJET  Vol.3 No.4 C , December 2015
Determination of Tea Saponin in Camellia Seed Oil with UV and HPLC Analysis
Abstract: A simple procedure is described for the HPLC and UV determination of tea saponin in tea seed oil. Determinate was accomplished with UV wavelength detection 550 nm for saponification sample, and HPLC was done under conditions: C18 analytical column of TC-C18, 4. 6 × 250 mm, column temperature at room temperature, injected sample volume was 10 μL, mobile phase’s methanol, flow-rate 0.8 ml/min and detection wavelength 280 nm.
Cite this paper: Ahmed, H. and Wang, C. (2015) Determination of Tea Saponin in Camellia Seed Oil with UV and HPLC Analysis. World Journal of Engineering and Technology, 3, 30-37. doi: 10.4236/wjet.2015.34C004.

[1]   Carpenter Jr., A.P. (1979) Determination of Tocopherols in Vegetable Oils. Journal of the American Oil Chemists Society, 5, 668-671.

[2]   Kelvin, O.G., Thomas, K.W., Okong’o, K.M. and Francis, W. (2013) Extraction and Analysis of Tea (Camellia sinensis) Seed Oil from Different Clones in Kenya. African Journal of Biotechnology.

[3]   Peter, J.L. and Di Gioia, J. (2009) Characterization of Tea Seed Oil for Quality Control and Authentication. Waters Journal.

[4]   Ma, L., Zhong, H. and Chen, Y. (2012) Study on Tea Saponin Extraction from Shell of Oil-Tea Camellia Seeds. International Conference on Mechanical Engineering and Material Science.

[5]   Yan, Y., Wu, Z.L., Zhao, Y.L. and Jiang, C.S. (2011) Separation of Tea Saponin by Two-Stage Foam Fractionation. Separation and Purification Technology.

[6]   Jie, L.H., Shao, P., Dan, F.H., Chang, L. and Ming, Y.X. (2012) Extraction of Saponin from Camellia oleifera Cake and Evaluation of Its Antioxidant Activity. International Journal of Food Science and Technology.

[7]   Dionisi, F., Prodolliet, J. and Tagliaferri, E. (1995) Assessment of Olive Oil Adulteration by Reversed-Phase High- Performance Liquid Chromatography/Amperometric Determination of Tocopherols and Tocotrienols. Journal of the American Oil Chemists Society, 72, 1505-1511.

[8]   IUPAC (1987) Standard Methods for The analysis of Oils, Fats and De-rivatives. Method 2.411 (7th Edition). International Union of Pure and Applied Chemistry, Blackwell Scientific Publi-cation, London.

[9]   Montedoro, G., Servili, M., Baldioli, M. and Miniati, E. (1992) Simple and Hydrolyzable Phenolic Compounds in Virgin Olive Oil. 1. Their Extraction, Separation, and Quantitative and Semiquantitative Evaluation by HPLC. Journal of Agriculture and Food Chemistry, 40, 1571-1576.

[10]   Pirisi, F.M., Angioni, A., Cabras, P., Garau, V.L., Di Teulada, M.T.S., dos Santos, M.K. and Bandino, G. (1997) Phenolic Compounds in Virgin Olive Oils. 1. Low-Wavelength Quantitative Determination of Complex Phenols by High- Performance Liquid Chromatography under Isocratic Elution. Journal of Chromatography A, 768, 207-213.

[11]   Hoehler, D., Frohlich, A.A., Marquardt, R.R. and Stelsovsky, H. (1998) Extraction of Tocopherol from Serum Prior to Reversed-Phase Liquid Chromatography. Journal of Agriculture and Food Chemistry, 46, 973-978.

[12]   Andrikopoulos, N.K., Brueschweller, H., Felber, H. and Taeschler, Ch. (1991) HPLC Analysis of Phenolic Antioxidants, Tocopherols and Triglycerides. Journal of the American Oil Chemists Society, 68, 359-364.

[13]   Indyk, H. and Woollard, D.C. (1986) Antioxidant Analysis in Edible Oils and Fats by Normal-Phase High-Perfor- mance Liquid Chromatography. Journal of Chromatography, 356, 401-408.

[14]   Gao, Z., Liu, X., Zhou, Y. and Wu, M. (2007) Study on Phy-tosterol Extraction from Crude Rapeseed Oil. Academic Periodical of Farm Products Processing.

[15]   Felix, M.C. (2007) Chinese Herbal Drug Research Trends. P. 152.

[16]   Li, X.Q., Ji, C., Sun, Y.Y., Yang, M.L. and Chu, X.G. (2008) Analysis of Synthetic Antioxidants and Preservatives in Edible Vegetable Oil by HPLC/TOF-MS Food Chemi-stry.

[17]   Zhong, S., He, G.W., Zhang, C.J., Hua, H. and Yan, Q.Y. (2008) RP2HPLC Determination of Tea Saponin in Tea2 Seed Cake. China Academic Journal.

[18]   Tasioula-Margari, M. and Okogeri O. (2001) Simultaneous Determination of Phenolic Compounds and Tocopherols in Virgin Olive Oil Using HPLC and UV Detection. Food Chemistry.

[19]   Okogeri, O. and Tasioula-Margari, M. (2000) Evaluation of Greek Virgin Olive Oil Phenolic Compounds by HPLC/ UV and GC/MS. In: 2000 IFT Annual Meeting Book of Abstracts, Illinois: Institute of Food Technologists, Dallas, Texas, 113.

[20]   Zhu, X. (2012) Transesterified Soybean Oil Technology and Establishment of Rapid Determination of Phytosterol. Master Thesis, Northeast Agricultural University.