AJPS  Vol.6 No.19 , December 2015
Determination of the Genetic Structure of the Oleaginous Lagenaria siceraria of the Nangui Abrogoua University Germplasm Collection
Abstract: Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection were analyzed using three microsatellite markers. The average Polymorphism Information Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared to the average expected heterozygosity value (He = 0.645) did not show significant differences in the selected accessions, which suggested random mating in the set of accessions. Within accession inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance (AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation resides among accessions and 61% within accessions. Accession structuring pattern derived from Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was suggested.
Cite this paper: Gbotto, A. , Koffi, K. , Baudoin, J. and Zoro Bi, I. (2015) Determination of the Genetic Structure of the Oleaginous Lagenaria siceraria of the Nangui Abrogoua University Germplasm Collection. American Journal of Plant Sciences, 6, 3231-3243. doi: 10.4236/ajps.2015.619314.

[1]   Mujaju, C. and Nybom, H. (2011) Local-Level Assessment of Watermelon Genetic Diversity in a Village in Masvingo Province, Zimbabwe: Structure and Dynamics of Landraces on Farm. African Journal of Agricultural Research, 6, 5822-5834.

[2]   Beevy, S.S. and Kuriachan, P. (1996) Chromosome Numbers of South Indian Cucurbitaceae and a Note on the Cytological Evolution in the Family. The Journal of Cytology and Genetics, 31, 65-71.

[3]   Burkill, H.M. (1985) The Useful Plants of West Tropical Africa. Royal Botanic Gardens, Kew, Richmond, Surrey, UK.

[4]   Lee, J.M. (1994) Cultivation of Grafted Vegetables I. Current Status, Grafting Methods and Benefits. Hort Science, 29, 235-239.

[5]   Yetisir, H. and Sari, N. (2003) Effect of Different Rootstock on Plant Growth, Yield and Quality of Watermelon. Australian Journal of Experimental Agriculture, 43, 1269-1274.

[6]   Whitaker, T.W. (1971) Endemism and Pre-Columbian Migration of Bottle Gourd, Lagenaria siceraria(Mol.) Standl. In: Riley, C.L.K.J.C., Pennington, C.W. and Runds, R.L., Eds., Man across the Sea, University of Press, Austin, 64-69.

[7]   Morimoto, Y., Maundu, P., Fujimaki, H. and Morishima, H. (2005) Diversity of Landraces of the White-Flowered Gourd (Lagenaria siceraria) and Its Wild Relatives in Kenya: Fruit and Seed Morphology. Genetic Resources and Crop Evolution, 52, 737-747.

[8]   Whitaker, T.W. (1971) Endemism and Pre-Columbian Migration of Bottle Gourd, Lagenaria siceraria (Mol.) Standl. In: Riley, C.L., Kelley, J.C., Pennington, C.W. and Runds, R.L., Eds., Man across the Sea, University of Texas Press, Austin, 78-218.

[9]   Achu, M.B., Fokou, E., Tchiégang, C., Fotso, M. and Tchouanguep, M.F. (2005) Nutritive Value of Some Cucurbitaceae Oilseeds from Different Regions in Cameroon. African Journal of Biotechnology, 4, 1329-1334.

[10]   Achigan-Dako, E.G., Fanou, N., Kouke, A., Avohou, H., Vodouhe, S.R. and Ahanchede, A. (2006) Evaluation agronomique de trois espèces de Egusi (Cucurbitaceae) utilisées dans l'alimentation au Bénin et élaboration d'un modèle de prédiction du rendement. Biotechnology, Agronomy, Society and Environment, 10, 121-129.

[11]   Zoro Bi, I.A., Koffi, K.K., Djè, Y., Malice, M. and Baudoin, J.P. (2006) Indigenous Cucurbits of Côte d’Ivoire: A Review of Their Genetic Resources. Sciences & Nature, 3, 1-9.

[12]   Montes-Hernandez, S. and Eguiarte, L.E. (2002) Genetic Structure and Indirect Estimates of Gene Flow in Three Taxa of Cucurbita (Cucurbitaceae) in Western Mexico. American Journal of Botany, 89, 1156-1163.

[13]   Gusmini, G. and Wehner, T.C. (2003) Polygenic Inheritance of Some Vine Traits in Two Segregating Watermelon Families. Cucurbit Genetics Cooperative Report, 26, 32-35.

[14]   Al-Maskri, A.Y., Khan, M.M., Iqbal, M.J. and Abbas, M. (2004) Germinability, Vigour and Electrical Conductivity Changes in Acceleratedly Aged Watermelon (Citrullus lanatus T.) Seeds. Journal of Food, Agriculture and Environment, 2, 100-103.

[15]   Koffi, K.K., Anzara, G.K., Malice, M., Djè, Y., Bertin, P., Baudoin, J.P. and Zoro Bi, I.A. (2009) Morphological and Allozyme Variation in a Collection of Lagenaria siceraria (Molina) Standl. from Côte d’Ivoire. Biotechnology, Agronomy, Society and Environment, 13, 257-270.

[16]   Xu, P., Wu, X., Luo, J., Wang, B., Liu, Y., Ehlers, J.D., Wang, S., Lu, Z. and Li, G. (2011) Partial Sequencing of the Bottle Gourd Genome Reveals Markers Useful for Phylogenetic Analysis and Breeding. BMC Genomics, 12, 467.

[17]   Blair, M.W., Panaud, O. and Mc Couch, S.R. (1999) Inter-Simple Sequence Repeat (ISSR) Amplification for Analysis of Microsatellite Motif Frequency and Fingerprinting in Rice (Oryza sativa L.). Theoretical and Applied Genetics, 98, 780-792.

[18]   Creste, S., Neto, A. and Figuera, A. (2001) Detection of Single Sequence Repeat Polymorphisms in Denaturing Polyacrylamide Sequencing Gels by Silver Staining. Plant Molecular Biology Reporter, 19, 299-306.

[19]   Guo, X. and Elston, R.C. (1999) Linkage Information Content of Polymorphic Genetic Markers. Human Heredity, 49, 112-118.

[20]   Peakall, R. and Smouse, P.E. (2006) GENALEX 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research. Molecular Ecology Notes, 6, 288-295.

[21]   StatSoft (2005) STATISTICA, logiciel d’analyse de données. 7.1. Edition.

[22]   Rousset, F. (2008) Genepop’007: A Complete Re-Implementation of the Genepop Software for Windows and Linux. Molecular Ecological Resources, 8, 103-106.

[23]   Dempster, A.P., Laird, N.M. and Rubin, D.B. (1977) Maximum Likelihood from Incomplete Data via the EM Algorithm. Journal of the Royal Statistical Society: Series B (Statistical Methodology), 39, 1-38.

[24]   Excoffier, L., Smouse, P.E. and Quattro, J.M. (1992) Analysis of Molecular Variance Inferred from Metric Distances among DNA Haplotypes: Application to Human Mitochondrial DNA Restriction Data. Genetics, 131, 479-491.

[25]   Pritchard, J.K., Stephens, M. and Donnelly, P. (2000) Inference of Population Structure Using Multilocus Genotype Data. Genetics, 155, 945-959.

[26]   Felsenstein, J. (1995) Phylogeny Inference Package (PHYLIP). 3.57c Edition, University of Washington, Seattle.

[27]   Nei, M. (1972) Genetic Distance between Populations. American Naturalist, 106, 283-292.

[28]   Page, R.D.M. (1996) Tree View: An Application to Display Phylogenetic Trees on Personal Computers. Computer Applications in the Biosciences, 12, 357-358.

[29]   Mujaju, C., Sehic, J., Werlemark, G., Garkava-Gustavsson, L., Fatih, M. and Nybom, H. (2010) Genetic Diversity in Watermelon (Citrullus lanatus) Landraces from Zimbabwe Revealed by RAPD and SSR Markers. Hereditas, 147, 142-153.

[30]   Ram, H.H., Sharma, K. and Jaiswal, H.R. (2006) Molecular Characterization of Promising Genotypes in Bottle Gourd including a Novel Segmented Leaf Type through RAPD. Vegetable Science, 33, 1-4.

[31]   Gürcan, K., Say, A., Yetisir, H. and Denli, N. (2015) A Study of Genetic Diversity in Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Population, and Implication for the Historical Origins on Bottle Gourds in Turkey. Genetic Resources and Crop Evolution, 62, 321-333.

[32]   Swerdlow, H. and Gestland, R. (1990) Capillary Gel Electrophoresis for Rapid, High Resolution DNA Sequencing. Nucleic Acids Research, 18, 1415-1419.

[33]   Sefc, K.M., Regner, F., Tureschek, E., Glössi, J. and Steinkellner, H. (1999) Identification of Microsatellite Sequences in Vitis riparia and Their Applicability for Genotyping of Different Vitis Species. Genome, 42, 367-373.

[34]   Minsart, L.-A., Zoro Bi, I.A., Dje, Y., Baudoin, J.-P., Jacquemart, A.-L. and Bertin, P. (2011) Set up of Simple Sequence Repeat Markers and First Investigation of the Genetic Diversity of West-African Watermelon (Citrullus lanatus ssp. vulgaris Oleaginous Type). Genetic Resources and Crop Evolution, 58, 805-814.

[35]   Koffi, K.K., Gbotto, A.A., Malice, M., Djè, Y., Bertin, P., Baudoin, J.P. and Zoro Bi, I.A. (2008) Morphological and Allozyme Variation in a Collection of Cucumeropsis mannii Naudin (Cucurbitaceae) from Côte d’Ivoire. Biochemical Systematis and Ecology, 36, 777-789.

[36]   Yetisir, H., Sakar, M. and Serçe, S. (2008) Collection and Morphological Characterization of Lagenaria siceraria Germplasm from the Mediterranean Region of Turkey. Genetic Resources and Crop Evolution, 55, 1257-1266.

[37]   Uluturk, Z.I., Frary, A. and Doganlar, S. (2011) Determination of Genetic Diversity in Watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] Germplasm. Australian Journal of Crop Science, 5, 1832-1836.