Back
 AJPS  Vol.6 No.19 , December 2015
High Light Intensity Increases the CAM Expression in “MD-2” Micro-Propagated Pineapple Plants at the End of the Acclimatization Stage
René C. Rodríguez-Escriba1*, Romelio Rodríguez1, Dariel López1, Gustavo Y. Lorente1, Yaima Pino1, Carlos E. Aragón1, Yolanda Garza2, Florencio E. Podestá3, Justo L. González-Olmedo1
Show more
Abstract: This work describes the evaluation of morpho-physiological and biochemical changes in “MD-2” micro-propagated pineapple plants (Ananas comosus (L.) Merr.) grown after 30 days under low light intensity (LL, greenhouse light conditions at 250 μmol·m-2·s-1) or high light intensity (HL, field light conditions at 800 μmol·m-2·s-1). Gas exchange, leaf pH, protein content and superoxide dismutase activity (SOD) (EC 1.15.1.1) were measured every 3 h during one day. Chlorophylls content and succulence index (SI) were determined at 9 h. Results showed significant differences in CO2 exchange rates, with a maximum occurring at 6 h (3.00 and 8.25 μmol CO2 m-2·s-1 for leaves under LL and HL conditions respectively). Plants under HL conditions had higher CO2 uptake and lower pH values between 0 h and 6 h respective to LL plants. The maximum pH value was attained 3 h before in HL plants. Leaf SI was increased and chlorophyll content decreased by HL conditions. SOD activity was higher in plants under HL conditions, near doubling those of LL plants at 18 h (2.8 versus 1.5 U·mg-1 Protein respectively). Both groups showed a typical CAM phenotype, but it was stronger in HL conditions, which may confer these plants with a better acclimation to transfer to the field.
Keywords: Crassulacean Acid Metabolism, Hardening Stage, Ex Vitro, Vitroplants, Gaseous Exchange
Cite this paper: Rodríguez-Escriba, R. , Rodríguez, R. , López, D. , Lorente, G. , Pino, Y. , Aragón, C. , Garza, Y. , Podestá, F. and González-Olmedo, J. (2015) High Light Intensity Increases the CAM Expression in “MD-2” Micro-Propagated Pineapple Plants at the End of the Acclimatization Stage. American Journal of Plant Sciences, 6, 3109-3118. doi: 10.4236/ajps.2015.619303.
References

[1]   FAOSTAT (2013) Food and Agriculture Organization of the United Stated Nations (FAOSTAT). Helping to Build a World without Hunger.
http://faostat3.fao.org/

[2]   Borland, A.M., Griffiths, H., Hartwell, J. and Smith, J.A. (2009) Exploiting the Potential of Plants with Crassulacean Acid Metabolism for Bioenergy Production on Marginal Lands. Journal of Experimental Botany, 60, 2879-2896.
http://www.ncbi.nlm.nih.gov/pubmed/19395392
http://dx.doi.org/10.1093/jxb/erp118

[3]   Escalona, M., Samson, G., Borroto, C. and Desjardins, Y. (2003) Physiology of Effects of Temporary Immersion Bioreactors on Micropropagated Pineapple Plantlets. In Vitro Cellular & Developmental Biology-Plant, 39, 651-656.
http://dx.doi.org/10.1079/IVP2003473

[4]   Almeida, W.A.B., Santana, G.S., Rodriguez, A.P.M. and Costa, M.A.P.C. (2002) Optimization of a Protocol for the Micropropagation of Pineapple. Revista Brasileira de Fruticultura, 24, 296-300.
http://ref.scielo.org/9s3jcj
http://dx.doi.org/10.1590/S0100-29452002000200005

[5]   González-Olmedo, J.L., Fundora, Z., Molina, L.A., Abdulnour, J., Desjardins, Y. and Escalona, M. (2005) New Contributions to Propagation of Pineapple (Ananas comosus (L.) Merr.) in Temporary Immersion Bioreactors. In Vitro Cellular & Developmental Biology-Plant, 41, 87-90.
http://dx.doi.org/10.1079/IVP2004603

[6]   Rodríguez, R., Becquer, R., Pino, Y., Rodríguez-Escriba, R.C. and López, D. (2013) Introduction of Pineapple Vitroplantas to Field Conditions in Collaboration with Farmers. Preliminary Results. Newsletter of the Pineapple Working Group, International Society for Horticultural Science, 20, 51-56.

[7]   Rodriguez, R., Aragon, C.E., Escalona, M., Gonzalez-Olmedo, J.L. and Desjardins, Y. (2008) Carbon Metabolism in Leaves of Micropropagated Sugarcane during Acclimatization Phase. In Vitro Cellular & Developmental Biology-Plant, 44, 533-539.
http://dx.doi.org/10.1007/s11627-008-9142-1

[8]   Yanes, E., González-Olmedo, J.L. and Rodríguez, R. (2000) A Technology of Acclimatization of Pineapple Vitroplants. Newsletter of the Pineapple Working Group, International Society for Horticultural Science, 15, 24-35.

[9]   Gonzalez-Olmedo, J.L., Coll, F. and Nuñez, M. (2005) A Role for Brassinosteroids during Acclimatization of Pineapple Plantlets. Newsletter of the Pineapple Working Group, International Society for Horticultural Science, 12, 17-20.

[10]   Villalobos, A., González, J., Santos, R. and Rodríguez, R. (2012) Morpho-Physiological Changes in Pineapple Plantlets [Ananas comosus (L.) merr.] during Acclimatization. Ciência e Agrotecnologia, 36, 624-630.
http://ref.scielo.org/s7dgk5
http://dx.doi.org/10.1590/S1413-70542012000600004

[11]   Nievola, C.C., Kraus, J.E., Freschi, L., Sousa, B.M. and Mercier, H. (2005) Temperature Determines the Occurrence of CAM or C3 Photosynthesis in Pineapple Plantlets Grown in Vitro. In Vitro Cellular & Developmental Biology-Plant, 41, 832-837.
http://dx.doi.org/10.1079/IVP2005694

[12]   Freschi, L., Rodrigues, M.A., Domingues, D.S., Purgatto, E., Van Sluys, M.A., Magalhaes, J.R., Kaiser, W.M. and Mercier, H. (2010) Nitric Oxide Mediates the Hormonal Control of Crassulacean Acid Metabolism Expression in Young Pineapple Plants. Plant Physiology, 152, 1971-1985.
http://www.ncbi.nlm.nih.gov/pubmed/20147491
http://dx.doi.org/10.1104/pp.109.151613

[13]   Aragón, C., Carvalho, L., González-Olmedo, J.L., Escalona, M. and Amancio, S. (2012) The Physiology of ex Vitro Pineapple (Ananas comosus (L.) Merr. var MD-2) as CAM or C3 Is Regulated by the Environmental Conditions. Plant Cell Report, 31, 757-769.
http://www.ncbi.nlm.nih.gov/pubmed/22134875
http://dx.doi.org/10.1007/s00299-011-1195-7

[14]   Aragón, C., Pascual, P., González-Olmedo, J.L., Escalona, M., Carvalho, L. and Amancio, S. (2013) The Physiology of ex Vitro Pineapple (Ananas comosus (L.) Merr. var MD-2) as CAM or C3 Is Regulated by the Environmental Conditions: Proteomic and Transcriptomic Profiles. Plant Cell Report, 32, 1807-1818.
http://www.ncbi.nlm.nih.gov/pubmed/23959598
http://dx.doi.org/10.1007/s00299-013-1493-3

[15]   Bartholomew, D.P., Paul, R. and Rohrbach, K. (2003) The Pineapple. Botany, Production and Uses. CABI, Wallingford.
http://dx.doi.org/10.1079/9780851995038.0000

[16]   Nelson, E.A. and Sage, R.F. (2008) Functional Constraints of CAM Leaf Anatomy: Tight Cell Packing Is Associated with Increased CAM Function across a Gradient of CAM Expression. Journal of Experimental Botany, 59, 1841-1850.
http://jxb.oxfordjournals.org/content/59/7/1841.abstract
http://dx.doi.org/10.1093/jxb/erm346

[17]   Lüttge, U. (2010) Ability of Crassulacean Acid Metabolism Plants to Overcome Interacting Stresses in Tropical Environments. AoB Plants, 2010, 1-9.
http://www.ncbi.nlm.nih.gov/pubmed/22476063
http://dx.doi.org/10.1093/aobpla/plq005

[18]   Prigge, M. and Guriérrez-Soto, M.V. (2014) Pineapple Photosynthesis and Leaf Sap pH as a Surrogate of CAM Performance in the Field. A Research Advance. Newsletter of the Pineapple Working Group, International Society for Horticultural Science, 21, 18-23.

[19]   Escalona, M., Lorenzo, J.C., González, B., Daquinta, M., González, J.L., Desjardins, Y. and Borroto, C.G. (1999) Pineapple (Ananas comusus L. Merr) Micropropagation in Temporary Immersion Systems. Plant Cell Report, 18, 743-748.
http://dx.doi.org/10.1007/s002990050653

[20]   Porra, R.J. (2002) The Chequered History of the Development and Use of Simultaneous Equations for the Accurate Determination of Chlorophylls a and b. Photosynthesis Research, 73, 149-156.
http://dx.doi.org/10.1023/A:1020470224740

[21]   Herrera, A. (2009) Crassulacean Acid Metabolism and Fitness under Water Deficit Stress: If Not for Carbon Gain, What Is Facultative CAM Good for? Annals of Botany, 103, 645-653.
http://www.ncbi.nlm.nih.gov/pubmed/18708641
http://dx.doi.org/10.1093/aob/mcn145

[22]   Bradford, M. (1976) A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein Dye Binding. Analytical Biochemistry, 72, 248-254.
http://dx.doi.org/10.1016/0003-2697(76)90527-3

[23]   McCord, J.M. and Fridovich, I. (1969) Superoxide Dismutase: An Enzymic Function for Erythrocuprein (Hemocuprein). Journal of Biological Chemistry, 244, 6049-6055.
http://www.jbc.org/content/244/22/6049.abstract

[24]   Pérez, C. (2005) Técnicas estadísticas con SPSS 12. Aplicaciones al análisis de datos. Pearson Educación, Madrid.

[25]   Cushman, J.C. and Bohnert, H.J. (1999) Crassulacean Acid Metabolism: Molecular Genetics. Annual Review of Plant Biology, 50, 305-332.
http://www.ncbi.nlm.nih.gov/pubmed/15012212
http://dx.doi.org/10.1146/annurev.arplant.50.1.305

[26]   Wild, B., Wanek, W., Postl, W. and Richter, A. (2010) Contribution of Carbon Fixed by RubisCO and PEPC to Phloem Export in the Crassulacean Acid Metabolism Plant Kalanchoe daigremontiana. Journal of Experimental Botany, 61, 1375-1383.
http://www.ncbi.nlm.nih.gov/pubmed/20159885
http://dx.doi.org/10.1093/jxb/erq006

[27]   Dodd, A.N., Borland, A.M., Haslam, R.P., Griffiths, H. and Maxwell, K. (2002) Crassulacean Acid Metabolism: Plastic, Fantastic. Journal of Experimental Botany, 53, 569-580.
http://www.ncbi.nlm.nih.gov/pubmed/11886877
http://dx.doi.org/10.1093/jexbot/53.369.569

[28]   Kluge, M. and Ting, I. (1978) Crassulacean Acid Metabolism: Analysis of an Ecological Adaptation. Springer-Verlag, Berlin.
http://dx.doi.org/10.1007/978-3-642-67038-1

[29]   Lüttge, U. (2004) Ecophysiology of Crassulacean Acid Metabolism (CAM). Annals of Botany, 93, 629-652.
http://www.ncbi.nlm.nih.gov/pubmed/15150072
http://dx.doi.org/10.1093/aob/mch087

[30]   Jia, H., Liggins, J.R. and Chow, W.S. (2012) Acclimation of Leaves to Low Light Produces Large Grana: The Origin of the Predominant Attractive Force at Work. Philosophical Transactions of the Royal Society B: Biological Sciences, 367, 3494-3502.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497075/
http://dx.doi.org/10.1098/rstb.2012.0071

[31]   Nobel, P.S. (2009) Physicochemical and Environmental Plant Physiology. 4th Edition, Academic Press, San Diego.

[32]   Chen, L.S. and Nose, A. (2004) Day-Night Changes of Energy-Rich Compounds in Crassulacean Acid Metabolism (CAM) Species Utilizing Hexose and Starch. Annals of Botany, 94, 449-455.
http://www.ncbi.nlm.nih.gov/pubmed/15277250
http://dx.doi.org/10.1093/aob/mch165

[33]   Ceusters, J., Borland, A.M., Taybi, T., Frans, M., Godts, C. and De Proft, M.P. (2014) Light Quality Modulates Metabolic Synchronization over the Diel Phases of Crassulacean Acid Metabolism. Journal of Experimental Botany, 65, 3705-3714.
http://www.ncbi.nlm.nih.gov/pubmed/24803500
http://dx.doi.org/10.1093/jxb/eru185

[34]   Luttge, U. (2000) The Tonoplast Functioning as the Master Switch for Circadian Regulation of Crassulacean Acid Metabolism. Planta, 211, 761-769.
http://www.ncbi.nlm.nih.gov/pubmed/11144260
http://dx.doi.org/10.1007/s004250000408

[35]   Borland, A.M., Elliott, S., Patterson, S., Taybi, T., Cushman, J., Pater, B. and Barnes, J. (2006) Are the Metabolic Components of Crassulacean Acid Metabolism Up-Regulated in Response to an Increase in Oxidative Burden? Journal of Experimental Botany, 57, 319-328.
http://www.ncbi.nlm.nih.gov/pubmed/16356942
http://dx.doi.org/10.1093/jxb/erj028

 
 
Top