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 AJPS  Vol.6 No.19 , December 2015
Antioxidant Activity and Phytochemical Profile of Spondias tuberosa Arruda Leaves Extracts
Abstract: Spondias tuberosa Arruda (umbuzeiro), a Brazilian semiarid plant, is a species of great economic, social, and ecological importance. In folk medicine, the leaves have been used in the treatment of diabetes, inflammation, stomach and uterine pains, and constipation. In this study, the antioxidant properties of ethyl acetate and methanol leaves extracts were evaluated in vitro using different methods: free radicals elimination by DPPH and ABTS assays, and transition metal reduction by phosphomolybdenium assay. In addition, a phytochemical study was also carried out. The methanolic leaves extracts showed the strongest antioxidant activity and the higher values for total phenolic and flavonoids. The results showed that S. tuberosa leaves have antioxidant activity and this seems to be related to the phenolic content.
Cite this paper: Uchôa, A. , Oliveira, W. , Pereira, A. , Silva, A. , Cordeiro, B. , Malafaia, C. , Almeida, C. , Silva, N. , Albuquerque, J. , Silva, M. and Correia, M. (2015) Antioxidant Activity and Phytochemical Profile of Spondias tuberosa Arruda Leaves Extracts. American Journal of Plant Sciences, 6, 3038-3044. doi: 10.4236/ajps.2015.619298.
References

[1]   Alves, L.F. (2013) Production of Phytotherapeutics in Brazil: History, Problems and Perspectives. Revista Virtual de Química, 5, 450-513. http://dx.doi.org/10.5935/1984-6835.20130038

[2]   Zardo, D.M., Zielinski, A.A.F., Alberti, A. and Nogueira, A. (2015) Phenolic Compounds and Antioxidant Capacity of Brazilian Apples. Food and Nutrition Sciences, 6, 727-735.
http://dx.doi.org/10.4236/fns.2015.68075

[3]   Wu, P.P., Ma, G.Z., Li, N.H., Deng, Q., Yin, Y.Y. and Huang, R.Q. (2015) Investigation of in Vitro and in Vivo Antioxidant Activities of Flavonoids Rich Extract from the Berries of Rhodomyrtustomentosa (Ait.) Hassk. Food Chemistry, 173, 194-202. http://dx.doi.org/10.1016/j.foodchem.2014.10.023

[4]   Niki, E. (2010) Assessment of Antioxidant Capacity in Vitro and in Vivo. Free Radical Biology and Medicine, 49, 503-515. http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.016

[5]   Roby, M.H.H., Sarhana, M.A., Selima, K.A.H. and Khalel, K.I. (2013) Evaluation of Antioxidant Activity, Total Phenols and Phenolic Compounds in Thyme (Thymus vulgaris L.), Sage (Salvia officinalis L.), and Marjoram (Origanummajorana L.) Extracts. Industrial Crops and Products, 43, 827-831.
http://dx.doi.org/10.1016/j.indcrop.2012.08.029

[6]   Omena, C.M.B., Valentim, I.B., Guedes, G.S., Rabelo, L.A., Mano, C.M., Bechara, E.J.H., Sawava, A.C.H.F., Trevisam, M.T.S., Costa, J.G., Ferreira, R.C.S., Sant’Ana, A.E.G. and Goulart, M.O.F. (2012) Antioxidant, Anti-Acetyl-cholinesterase and Cytotoxic Activities of Ethanol Extracts of Peel, Pulp and Seeds of Exotic Brazilian Fruits Antioxidant, Anti-Acetylcholinesterase and Cytotoxic Activities in Fruits. Food Research International, 49, 334-344.
http://dx.doi.org/10.1016/j.foodres.2012.07.010

[7]   Nadia, T.L., Machado, I.C. and Lopes, A.V. (2007) Pollination of Spondiastuberosa Arruda (Anacardiaceae) and Analysis of Pollinators Share with Ziziphusjoazeiro Mart. (Rhamnaceae), Fruit Species Endemic to the Caatinga. Brazilian Journal of Botany, 30, 89-100.
http://dx.doi.org/10.1590/S0100-84042007000100009

[8]   Giulietti, A.M., Harley, R.M., Queiroz, L.P., Barbosa, M.R.V., Bocage Neta, A.L. and Figueiredo, M.A. (2002) Espécies Endêmicas da Caatinga. Vegetação & Flora da Caatinga Associação Plantas do Nordeste—APNE, Centro Nordestino de Informação sobre Plantas—CNIP, Recife.

[9]   Araújo, E.L., Castro, C.C. and Albuquerque, U.P. (2007) Dynamics of Brazilian Caatinga e a Review Concerning Plants, Environment and People. Functional Ecosystems and Communities, 1, 15-28.
http://www.academia.edu/1191277/Dynamics_of_Brazilian_Caatinga_A_
review_concerning_the_plants_environment_and_people


[10]   Cavalcanti, N.B., Resende, G.M. and Brito, L.T.L. (2000) Processamento do fruto do imbuzeiro (Spondias tuberosa Arr. Cam.). Ciência e Agrotecnologia, 24, 252-259.
http://www.academia.edu/1191277/Dynamics_of_Brazilian_Caatinga_A_
review_concerning_the_plants_environment_and_people


[11]   Lins Neto, E.M.F., Peroni, N. and Albuquerque, U.P. (2010) Traditional Knowledge and Management of Umbu (Spondias tuberosa, Anacardiaceae): An Endemic Species from the Semie-Arid Region of Northeastern Brazil. Economic Botany, 64, 11-21. http://dx.doi.org/10.1007/s12231-009-9106-3

[12]   Barreto, L.S. (2007) Plano de manejo para conservação do umbuzeiro (Spondias tuberosa) e de seus polinizadores no Território Indígena Pankararé, Raso da Catarina. Dissertation, Bahia State University, Bahia.

[13]   Vidigal, M.C.T.R., Minim, V.P.R., Carvalho, N.B., Milagres, M.P. and Gonçalves, A.C.A. (2011) Effect of a Health Claim on Consumer Acceptance of Exotic Brazilian Fruit Juices: Açaí (Euterpe oleracea Mart.), Camu-Camu (Myrciaria dubia), Cajá (Spondias lutea L.) and Umbu (Spondias tuberose Arruda). Food Research International, 44, 1988- 1996. http://dx.doi.org/10.1016/j.foodres.2010.11.028

[14]   Rocha, E.A.L.S.S., Carvalho, A.V.O.R., Andrade, S.R.A., Medeiros, A.C.D., Trovão, D.M.B. and Costa, E.M.M.B. (2013) Potencial antimicrobiano de seis plantas do semiárido paraibano contra bactérias relacionadas à infecção endodôntica. Revista de Ciências Farmacêuticas Básica e Aplicada, 34, 351-355.
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewFile/2636/1461

[15]   Agra, M.F., Freitas, P.F. and Barbosa-Filho, J.M. (2007) Synopsis of the Plants Known as Medicinal and Poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia, 17, 114-140.

[16]   Harborne, J.B. (1998) Phytochemical Methods. 3rd Edition, Chapman & Hall, Londres.

[17]   Roberts, E.A.H., Cartwright, R.A. and Oldschool M. (1957) Phenolic Substances of Manufactured Tea. I. Fractionation and Paper Chromatography of Water-Soluble Substances. Science of Food and Agriculture, 8, 72-80.
http://dx.doi.org/10.1002/jsfa.2740080203

[18]   Li, A.B., Wonga, C.C., Ka-Wing, C. and Chen, F. (2008) Antioxidant Properties in Vitro and Total Phenolic Contents in Methanol Extracts from Medicinal Plants. Swiss Society of Food Science and Technology, 41, 385-390.

[19]   Woisky, R.G. and Salatino, A. (1998) Analysis of Propolis: Some Parameters and Procedures for Chemical Quality Control. Journal of Apicultural Research, 37, 99-105.
http://europepmc.org/abstract/AGR/IND21966817

[20]   Silva, R.A., Lima, M.S.F., Viana, J.B.M., Bezerra, V.S., Pimentel, M.C.B., Porto, A.L.F., Cavalcante, M.T.H. and Lima Filho, J.L. (2012) Can Artisanal ‘‘Coalho’’ Cheese from Northeastern Brazil Be Used as a Functional Food? Food Chemistry, 135, 1533-1538. http://dx.doi.org/10.1016/j.foodchem.2012.06.058

[21]   Brand-WIliams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. Food Science and Technology, 28, 25-30. http://dx.doi.org/10.1016/s0023-6438(95)80008-5

[22]   Prieto, P., Pineda, M. and Aguilar, M. (1999) Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry, 269, 337-341. http://dx.doi.org/10.1006/abio.1999.4019

[23]   Ji, H.F. and Zhang, H.Y. (2008) Multipotent Natural Agents to Combat Alzheimer’s Disease. Functional Spectrum and Structural Features. Acta Pharmacologica Sinica, 29, 143-151.
http://dx.doi.org/10.1111/j.1745-7254.2008.00752.x

[24]   Omena, C.M.B., Valentim, I.B., Guedes, G.S., Rabelo, L.A., Mano, C.M., Bechara, E.J.H., Sawaya, A.C.H.F., Trevisan, M.T.S., Costa, J.G., Ferreira, R.C.S., Sant’Ana, A.E.G. and Goulart, M.O.F. (2012) Antioxidant, Anti-Acetylcholinesterase and Cytotoxic Activities of Ethanol Extracts of Peel, Pulp and Seeds of Exotic Brazilian Fruits Antioxidant, Anti- Acetylcholinesterase and Cytotoxic Activities in Fruits. Food Research International, 49, 334-344.
http://dx.doi.org/10.1016/j.foodres.2012.07.010

[25]   Siddhuraju, P. and Becker, K. (2003) Antioxidant Properties of Various Solvent Extracts of Total Phenolic Constituents from Three Different Agroclimatic Origins of Drumstick Tree (Moringa oleiferea Lam.) Leaves. Journal of Agricultural and Food Chemistry, 51, 2144-2155.
http://dx.doi.org/10.1021/jf020444+

[26]   Roby, M.H.H., Sarhan, M.A., Selim, K.A.H. and Khalel, I.K. (2013) Evaluation of Antioxidant Activity, Total Phenols and Phenolic Compounds in Thyme (Thymus vulgaris L.), Sage (Salvia officinalis L.), and Marjoram (Origanum majorana L.) Extracts. Industrial Crops and Products, 43, 827-831. http://dx.doi.org/10.1016/j.indcrop.2012.08.029

[27]   Berlowski, A., Zawada, K., Wawer, I. and Paradowska, K. (2013) Antioxidant Properties of Medicinal Plants from Peru. Food and Nutrition Sciences, 4, 71-77. http://dx.doi.org/10.4236/fns.2013.48A009

[28]   Hossain, M.A. and Rahman, S.M.M. (2011) Total Phenolics, Flavonoids and Antioxidant Activity of Tropical Fruit Pineapple. Food Research International, 44, 672-676.
http://dx.doi.org/10.1016/j.foodres.2010.11.036

[29]   Garg, D., Shaikh, A., Muley, A. and Marar, T. (2012) In-Vitro Antioxidant Activity and Phytochemical Analysis in Extracts of Hibiscus rosa-sinensis Stem and Leaves. Free Radicals and Antioxidants, 2, 3-6.
http://dx.doi.org/10.5530/ax.2012.3.6

[30]   Arnao, M.B. (2000) Some Methodological Problems in the Determination of Antioxidant Activity Using Chromogen Radicals: A Practical Case. Trends in Food Science and Technology, 11, 419-421.
http://dx.doi.org/10.1016/S0924-2244(01)00027-9

[31]   Almeida, M.M.B., Sousa, P.H.M., Arriaga, A.M.C., Prado, G.M., Magalhães, C.E.C., Maia, G.A. and de Lemos, T.L.G. (2011) Bioactive Compounds and Antioxidant Activity of Fresh Exotic Fruits from Northeastern Brazil. Food Research International, 44, 2155-2159.
http://dx.doi.org/10.1016/j.foodres.2011.03.051

[32]   Loo, A.Y., Jain, K. and Darah, I. (2008) Antioxidant Activity of Compounds Isolated from the Pyroligneous Acid Rhizophora apiculata. Food Chemistry, 107, 1151-1160.
http://dx.doi.org/10.1016/j.foodchem.2007.09.044

[33]   Zengin, G., Uysal, S., Ceylan, R. and Aktumsek, A. (2015) Phenolic Constituent, Antioxidative and Tyrosinase Inhibitory Activity of Ornithogalum narbonense L. from Turkey: A Phytochemical Study. Industrial Crops and Products, 70, 1-6. http://dx.doi.org/10.1016/j.indcrop.2015.03.012

 
 
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