Antiviral Effect of Ribonuclease from Bacillus pumilus against Phytopathogenic Rna-Viruses

Margarita  Sharipova; Annett  Rockstroh; Nelly  Balaban; Ayslu  Mardanova; Anna  Toymentseva; Anastasiya  Tikhonova; Semen  Vologin; Zenon  Stashevsky

doi:10.4236/as.2015.611130

Margarita Sharipova¹, Annett Rockstroh¹, Nelly Balaban¹, Ayslu Mardanova¹, Anna Toymentseva¹, Anastasiya Tikhonova¹, Semen Vologin², Zenon Stashevsky²

Abstract: Background: Viruses can cause different diseases in plants. To prevent viral infections, plants are treated with chemical compounds and antiviral agents. Chemical antiviral agents usually have narrow specificity, which limits their wide application. Alternative antiviral strategy is associated with the use of microbial enzymes, which are less toxic and are readily decomposed without accumulation of harmful substances. The aim of this work is to study the effect of Bacillus pumilus ribonuclease on various phytopathogenic viruses with specific focus on the ability of enzyme to eliminate them from plant explants in vitro. Materials and methods: Extracellular ribonuclease of B. pumilus is tested as an antiviral agent. To study the antiviral effect of RNase, depending on concentration and the time of application several plant-virus model systems are used. Virus detection is conducted by serological testing and RT-PCR. Results: Bacillus pumilus ribonuclease possesses antiviral activity against plant Rna-viruses RCMV (red clover mottle virus), PVX (Potato Virus X) and AMV (Alfalfa Mosaic Virus). The maximum inhibitory effect against actively replicating viruses is observed when plants are treated with the enzyme in the concentration of 100 ug/ml prior to infection. In case of local necrosis ribonuclease in the concentration of 1 ug/ml completely inhibits the development of RCMV virus on bean plants. The enzyme is able to penetrate plants and inhibit the development of viral infection, inhibiting effect for untreated surfaces decreased on average for 20%. It is also found that B. pumilus ribonuclease protects apical explants of sprouts of potato tubers from PVM and PVS viruses. Conclusion: B. pumilus ribonuclease possesses antiviral activity against plant Rna-viruses and produces viruses-free plants in the apical meristem culture.

Keywords: Bacillus Pumilus, Ribonuclease, Phytopathogenic Rna-Viruses, Inhibition, Virus-Free Apical

Cite this paper: Sharipova, M. , Rockstroh, A. , Balaban, N. , Mardanova, A. , Toymentseva, A. , Tikhonova, A. , Vologin, S. and Stashevsky, Z. (2015) Antiviral Effect of Ribonuclease from Bacillus pumilus against Phytopathogenic Rna-Viruses. Agricultural Sciences, 6, 1357-1366. doi: 10.4236/as.2015.611130.

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