Can Improve Iron Biofortification Antioxidant Response, Yield and Nutritional Quality in Green Bean?
Author(s)
Juan P. Sida-Arreola1,
Esteban Sánchez1*,
Graciela D. Ávila-Quezada2,
Paul B. Zamudio-Flores1,
Carlos H. Acosta-Muñiz1
Affiliation(s)
1 Centro de Investigación en Alimentación y Desarrollo, Chihuahua, México. 2 Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Chihuahua, México.
1 Centro de Investigación en Alimentación y Desarrollo, Chihuahua, México. 2 Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Chihuahua, México.
ABSTRACT
The aim of this study was to evaluate the effect of iron biofortification on antioxidant response, yield and nutritional qualityof green bean (Phaseolus vulgaris L.) under greenhouse conditions. Fe was applied using two forms (FeSO4 and Fe-EDDHA) at four doses of application (0, 25, 50 and 100 μm) added under a hydroponic system, and were tested over a period of 40 days. The Fe content was assessed in seeds, as well as the activity of antioxidant enzymes, production of H2O2, yield and nutritional quality. The results being obtained indicated that the accumulation of Fe in bean seeds enhanced with the application of Fe-EDDHA at the dose of 25 μm. This demonstrated that low Fe application dose was enough to increase Fe levels in seeds of common bean. In addition, Fe-EDDHA application form at 50 μmol was the best treatment to improve crop yield. Respect to antioxidant system, chelated form of Fe (Fe-EDDHA) was more effective in the activation of antioxidant enzymes (CAT, SOD and GSH-PX), and a lower content of H2O2 in green bean seeds. Finally, to raise the Fe concentration in bean under biofortification program was a promising strategy in cropping systems in order to increase the ingestion of iron and antioxidant capacity in the general population and provided the benefits that this element offered in human health.
The aim of this study was to evaluate the effect of iron biofortification on antioxidant response, yield and nutritional qualityof green bean (Phaseolus vulgaris L.) under greenhouse conditions. Fe was applied using two forms (FeSO4 and Fe-EDDHA) at four doses of application (0, 25, 50 and 100 μm) added under a hydroponic system, and were tested over a period of 40 days. The Fe content was assessed in seeds, as well as the activity of antioxidant enzymes, production of H2O2, yield and nutritional quality. The results being obtained indicated that the accumulation of Fe in bean seeds enhanced with the application of Fe-EDDHA at the dose of 25 μm. This demonstrated that low Fe application dose was enough to increase Fe levels in seeds of common bean. In addition, Fe-EDDHA application form at 50 μmol was the best treatment to improve crop yield. Respect to antioxidant system, chelated form of Fe (Fe-EDDHA) was more effective in the activation of antioxidant enzymes (CAT, SOD and GSH-PX), and a lower content of H2O2 in green bean seeds. Finally, to raise the Fe concentration in bean under biofortification program was a promising strategy in cropping systems in order to increase the ingestion of iron and antioxidant capacity in the general population and provided the benefits that this element offered in human health.
Cite this paper
Sida-Arreola, J. , Sánchez, E. , Ávila-Quezada, G. , Zamudio-Flores, P. and Acosta-Muñiz, C. (2015) Can Improve Iron Biofortification Antioxidant Response, Yield and Nutritional Quality in Green Bean?. Agricultural Sciences, 6, 1324-1332. doi: 10.4236/as.2015.611127.
Sida-Arreola, J. , Sánchez, E. , Ávila-Quezada, G. , Zamudio-Flores, P. and Acosta-Muñiz, C. (2015) Can Improve Iron Biofortification Antioxidant Response, Yield and Nutritional Quality in Green Bean?. Agricultural Sciences, 6, 1324-1332. doi: 10.4236/as.2015.611127.
References
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http://dx.doi.org/10.1023/A:1024146710611 [2] Miklas, P.N., Kelly, J.D., Beebe, S.E. and Blair, M.W. (2006) Common Bean Breeding for Resistance against Biotic and Abiotic Stresses: From Classical to MAS Breeding. Euphytica, 147, 105-131.
http://dx.doi.org/10.1007/s10681-006-4600-5 [3] García-Bañuelos, M.L., Sida-Arreola, J.P. and Sánchez, E. (2014) Biofortification Is a Promising Approach to Increase the Content of Iron and Zinc in Staple Food Crops. Journal of Elementology, 19, 865-888. [4] Cakmak, I., Pfeiffer, W.H. and McClafferty, B. (2010) Review: Biofortification of Durum Wheat with Zinc and Iron. Cereal Chemistry, 87, 10-20.
http://dx.doi.org/10.1094/CCHEM-87-1-0010 [5] García-Bañuelos, M.L., Hermosillo-Cereceres, M.A. and Sánchez, E. (2011) The Importance of Selenium Biofortification in Food Crops. Current Nutrition and Food Science, 7, 181-190.
http://dx.doi.org/10.2174/157340111797264796 [6] Hermosillo-Cereceres, M.A., Sánchez, E., Guevara-Aguilar, A., Muñoz-Márquez, E. and García-Bañuelos, M.L. (2013) Biofortification and Distribution Patterns of Selenium in Bean: Response to Selenate and Selenite. Journal of Food, Agriculture and Environment, 11, 421-426. [7] Aciksoz, S.B., Yazici, A., Ozturk, L. and Cakmak, I. (2011) Biofortification of Wheat with Iron through Soil and Foliar Application of Nitrogen and Iron Fertilizers. Plant and Soil, 349, 215-225.
http://dx.doi.org/10.1007/s11104-011-0863-2 [8] Hänsch, R. and Mendel, R.R. (2009) Physiological Functions of Mineral Micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Current Opinion in Plant Biology, 12, 259-266.
http://dx.doi.org/10.1016/j.pbi.2009.05.006 [9] Minotti, G. and Aust, S. (1987) The Requirement for Iron (III) in the Initiation of Lipid Peroxidation by Iron (II) and Hydrogen Peroxide. Journal of Biological Chemistry, 262, 1098-1104. [10] Elstner, E.F. and Osswald, W. (1994) Mechanisms of Oxygen Activation during Plant Stress. Proceedings-Royal Sciety of Edinburgh B, 102, 131-131. [11] Alscher, R.G., Donahue, J.L. and Cramer, C.L. (1997) Reactive Oxygen Species and Antioxidants: Relationships in Green Cells. Physiologia Plantarum, 100, 224-233.
http://dx.doi.org/10.1111/j.1399-3054.1997.tb04778.x [12] Noctor, G. and Foyer, C.H. (1998) Ascorbate and Glutathione: Keeping Active Oxygen under Control. Annual Review of Plant Biology, 49, 249-279.
http://dx.doi.org/10.1146/annurev.arplant.49.1.249 [13] Vanlerberghe, G.C. and McIntosh, L. (1997) Alternative Oxidase: From Gene to Function. Annual Review of Plant Biology, 48, 703-734.
http://dx.doi.org/10.1146/annurev.arplant.48.1.703 [14] Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410.
http://dx.doi.org/10.1016/S1360-1385(02)02312-9 [15] Ríos, J., Blasco, B., Cervilla, L., Rosales, M., Sanchez-Rodriguez, E., Romero, L. and Ruiz, J. (2009) Production and Detoxification of H2O2 in Lettuce Plants Exposed to Selenium. Annals of Applied Biology, 154, 107-116.
http://dx.doi.org/10.1111/j.1744-7348.2008.00276.x [16] Karacan, M.S. and Aslantas, N. (2008) Simultaneous Preconcentration and Removal of Iron, Chromium, Nickel with N-Etylenebis-(Ethane Sulfonamide) Ligand on Activated Carbon in Aqueous Solution and Determination by ICP-OES. Journal of Hazardous Materials, 155, 551-557.
http://dx.doi.org/10.1016/j.jhazmat.2007.11.107 [17] Giannopolitis, C.N. and Ries, S.K. (1977) Superoxide Dismutases I. Occurrence in Higher Plants. Plant Physiology, 59, 309-314.
http://dx.doi.org/10.1104/pp.59.2.309 [18] Rao, M.V., Paliyath, G., Ormrod, D.P., Murr, D.P. and Watkins, C.B. (1997) Influence of Salicylic Acid on H2O2 Production, Oxidative Stress, and H2O2-Metabolizing Enzymes (Salicylic Acid-Mediated Oxidative Damage Requires H2O2). Plant Physiology, 115, 137-149.
http://dx.doi.org/10.1104/pp.115.1.137 [19] Flohé, L. and Günzler, W.A. (1984) Assays of Glutathione Peroxidase. Methods in Enzymology, 105, 114-120.
http://dx.doi.org/10.1016/S0076-6879(84)05015-1 [20] Brennan, T. and Frenkel, C. (1977) Involvement of Hydrogen Peroxide in the Regulation of Senescence in Pear. Plant Physiology, 59, 411-416.
http://dx.doi.org/10.1104/pp.59.3.411 [21] Sánchez, E., Soto, J.M., García, P.C., López-Lefebre, L.R., Rivero, R.M., Ruiz, J.M. and Romero, L. (2000) Phenolic Compounds and Oxidative Metabolism in Green Bean Plants under Nitrogen Toxicity. Functional Plant Biology, 27, 973-978.
http://dx.doi.org/10.1071/PP00008 [22] Caliskan, S., Ozkaya, I., Caliskan, M. and Arslan, M. (2008) The Effects of Nitrogen and Iron Fertilization on Growth, Yield and Fertilizer Use Efficiency of Soybean in a Mediterranean-Type Soil. Field Crops Research, 108, 126-132.
http://dx.doi.org/10.1016/j.fcr.2008.04.005 [23] Ortega-Blu, R. and Molina-Roco, M. (2007) Comparison between Sulfates and Chelated Compounds as Sources of Zinc and Iron in Calcareous Soils. Agrociencia, 41, 491-502. [24] Djanaguiraman, M., Devi, D.D., Shanker, A.K., Sheeba, J.A. and Bangarusamy, U. (2005) Selenium—An Antioxidative Protectant in Soybean during Senescence. Plant and Soil, 272, 77-86.
http://dx.doi.org/10.1007/s11104-004-4039-1 [25] Becana, M., Moran, J. and Iturbe-Ormaetxe, I. (1998) Iron-Dependent Oxygen Free Radical Generation in Plants Subjected to Environmental Stress: Toxicity and Antioxidant Protection. Plant and Soil, 201, 137-147.
http://dx.doi.org/10.1023/A:1004375732137 [26] Gupta, R., Kannan, G.M., Sharma, M. and Flora, S.J. (2005) Therapeutic Effects of Moringaoleifera on Arsenic-Induced Toxicity in Rats. Environmental Toxicology and Pharmacology, 20, 456-464.
http://dx.doi.org/10.1016/j.etap.2005.05.005 [27] Sun, B., Jing, Y., Chen, K., Song, L., Chen, F. and Zhang, L. (2007) Protective Effect of Nitric Oxide on Iron Deficiency-Induced Oxidative Stress in Maize (Zea mays). Journal of Plant Physiology, 164, 536-543.
http://dx.doi.org/10.1016/j.jplph.2006.02.011 [28] Slesak, I., Slesak, H., Libik, M. and Miszalski, Z. (2008) Antioxidant Response System in the Short-Term Post-Wounding Effect in Mesembryanthemum crystallinum Leaves. Journal of Plant Physiology, 165, 127-137.
http://dx.doi.org/10.1016/j.jplph.2007.03.015 [29] Arora, A., Sairam, R. and Srivastava, G. (2002) Oxidative Stress and Antioxidative System in Plants. Current Science, 82, 1227-1238.
[1] Broughton, W., Hernandez, G., Blair, M., Beebe, S., Gepts, P. and Vanderleyden, J. (2003) Beans (Phaseolus spp.)— Model Food Legumes. Plant and Soil, 252, 55-128.
http://dx.doi.org/10.1023/A:1024146710611 [2] Miklas, P.N., Kelly, J.D., Beebe, S.E. and Blair, M.W. (2006) Common Bean Breeding for Resistance against Biotic and Abiotic Stresses: From Classical to MAS Breeding. Euphytica, 147, 105-131.
http://dx.doi.org/10.1007/s10681-006-4600-5 [3] García-Bañuelos, M.L., Sida-Arreola, J.P. and Sánchez, E. (2014) Biofortification Is a Promising Approach to Increase the Content of Iron and Zinc in Staple Food Crops. Journal of Elementology, 19, 865-888. [4] Cakmak, I., Pfeiffer, W.H. and McClafferty, B. (2010) Review: Biofortification of Durum Wheat with Zinc and Iron. Cereal Chemistry, 87, 10-20.
http://dx.doi.org/10.1094/CCHEM-87-1-0010 [5] García-Bañuelos, M.L., Hermosillo-Cereceres, M.A. and Sánchez, E. (2011) The Importance of Selenium Biofortification in Food Crops. Current Nutrition and Food Science, 7, 181-190.
http://dx.doi.org/10.2174/157340111797264796 [6] Hermosillo-Cereceres, M.A., Sánchez, E., Guevara-Aguilar, A., Muñoz-Márquez, E. and García-Bañuelos, M.L. (2013) Biofortification and Distribution Patterns of Selenium in Bean: Response to Selenate and Selenite. Journal of Food, Agriculture and Environment, 11, 421-426. [7] Aciksoz, S.B., Yazici, A., Ozturk, L. and Cakmak, I. (2011) Biofortification of Wheat with Iron through Soil and Foliar Application of Nitrogen and Iron Fertilizers. Plant and Soil, 349, 215-225.
http://dx.doi.org/10.1007/s11104-011-0863-2 [8] Hänsch, R. and Mendel, R.R. (2009) Physiological Functions of Mineral Micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Current Opinion in Plant Biology, 12, 259-266.
http://dx.doi.org/10.1016/j.pbi.2009.05.006 [9] Minotti, G. and Aust, S. (1987) The Requirement for Iron (III) in the Initiation of Lipid Peroxidation by Iron (II) and Hydrogen Peroxide. Journal of Biological Chemistry, 262, 1098-1104. [10] Elstner, E.F. and Osswald, W. (1994) Mechanisms of Oxygen Activation during Plant Stress. Proceedings-Royal Sciety of Edinburgh B, 102, 131-131. [11] Alscher, R.G., Donahue, J.L. and Cramer, C.L. (1997) Reactive Oxygen Species and Antioxidants: Relationships in Green Cells. Physiologia Plantarum, 100, 224-233.
http://dx.doi.org/10.1111/j.1399-3054.1997.tb04778.x [12] Noctor, G. and Foyer, C.H. (1998) Ascorbate and Glutathione: Keeping Active Oxygen under Control. Annual Review of Plant Biology, 49, 249-279.
http://dx.doi.org/10.1146/annurev.arplant.49.1.249 [13] Vanlerberghe, G.C. and McIntosh, L. (1997) Alternative Oxidase: From Gene to Function. Annual Review of Plant Biology, 48, 703-734.
http://dx.doi.org/10.1146/annurev.arplant.48.1.703 [14] Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410.
http://dx.doi.org/10.1016/S1360-1385(02)02312-9 [15] Ríos, J., Blasco, B., Cervilla, L., Rosales, M., Sanchez-Rodriguez, E., Romero, L. and Ruiz, J. (2009) Production and Detoxification of H2O2 in Lettuce Plants Exposed to Selenium. Annals of Applied Biology, 154, 107-116.
http://dx.doi.org/10.1111/j.1744-7348.2008.00276.x [16] Karacan, M.S. and Aslantas, N. (2008) Simultaneous Preconcentration and Removal of Iron, Chromium, Nickel with N-Etylenebis-(Ethane Sulfonamide) Ligand on Activated Carbon in Aqueous Solution and Determination by ICP-OES. Journal of Hazardous Materials, 155, 551-557.
http://dx.doi.org/10.1016/j.jhazmat.2007.11.107 [17] Giannopolitis, C.N. and Ries, S.K. (1977) Superoxide Dismutases I. Occurrence in Higher Plants. Plant Physiology, 59, 309-314.
http://dx.doi.org/10.1104/pp.59.2.309 [18] Rao, M.V., Paliyath, G., Ormrod, D.P., Murr, D.P. and Watkins, C.B. (1997) Influence of Salicylic Acid on H2O2 Production, Oxidative Stress, and H2O2-Metabolizing Enzymes (Salicylic Acid-Mediated Oxidative Damage Requires H2O2). Plant Physiology, 115, 137-149.
http://dx.doi.org/10.1104/pp.115.1.137 [19] Flohé, L. and Günzler, W.A. (1984) Assays of Glutathione Peroxidase. Methods in Enzymology, 105, 114-120.
http://dx.doi.org/10.1016/S0076-6879(84)05015-1 [20] Brennan, T. and Frenkel, C. (1977) Involvement of Hydrogen Peroxide in the Regulation of Senescence in Pear. Plant Physiology, 59, 411-416.
http://dx.doi.org/10.1104/pp.59.3.411 [21] Sánchez, E., Soto, J.M., García, P.C., López-Lefebre, L.R., Rivero, R.M., Ruiz, J.M. and Romero, L. (2000) Phenolic Compounds and Oxidative Metabolism in Green Bean Plants under Nitrogen Toxicity. Functional Plant Biology, 27, 973-978.
http://dx.doi.org/10.1071/PP00008 [22] Caliskan, S., Ozkaya, I., Caliskan, M. and Arslan, M. (2008) The Effects of Nitrogen and Iron Fertilization on Growth, Yield and Fertilizer Use Efficiency of Soybean in a Mediterranean-Type Soil. Field Crops Research, 108, 126-132.
http://dx.doi.org/10.1016/j.fcr.2008.04.005 [23] Ortega-Blu, R. and Molina-Roco, M. (2007) Comparison between Sulfates and Chelated Compounds as Sources of Zinc and Iron in Calcareous Soils. Agrociencia, 41, 491-502. [24] Djanaguiraman, M., Devi, D.D., Shanker, A.K., Sheeba, J.A. and Bangarusamy, U. (2005) Selenium—An Antioxidative Protectant in Soybean during Senescence. Plant and Soil, 272, 77-86.
http://dx.doi.org/10.1007/s11104-004-4039-1 [25] Becana, M., Moran, J. and Iturbe-Ormaetxe, I. (1998) Iron-Dependent Oxygen Free Radical Generation in Plants Subjected to Environmental Stress: Toxicity and Antioxidant Protection. Plant and Soil, 201, 137-147.
http://dx.doi.org/10.1023/A:1004375732137 [26] Gupta, R., Kannan, G.M., Sharma, M. and Flora, S.J. (2005) Therapeutic Effects of Moringaoleifera on Arsenic-Induced Toxicity in Rats. Environmental Toxicology and Pharmacology, 20, 456-464.
http://dx.doi.org/10.1016/j.etap.2005.05.005 [27] Sun, B., Jing, Y., Chen, K., Song, L., Chen, F. and Zhang, L. (2007) Protective Effect of Nitric Oxide on Iron Deficiency-Induced Oxidative Stress in Maize (Zea mays). Journal of Plant Physiology, 164, 536-543.
http://dx.doi.org/10.1016/j.jplph.2006.02.011 [28] Slesak, I., Slesak, H., Libik, M. and Miszalski, Z. (2008) Antioxidant Response System in the Short-Term Post-Wounding Effect in Mesembryanthemum crystallinum Leaves. Journal of Plant Physiology, 165, 127-137.
http://dx.doi.org/10.1016/j.jplph.2007.03.015 [29] Arora, A., Sairam, R. and Srivastava, G. (2002) Oxidative Stress and Antioxidative System in Plants. Current Science, 82, 1227-1238.