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 FNS  Vol.6 No.15 , November 2015
Nutritional and Anti-Nutrient Composition of Three Kola Nuts ( Cola nitida, Cola acuminata and Garcinia kola ) Produced in Benin
Abstract: Kola nuts were regularly chewed by West Africans and Beninese in particularly. The aim of this study was to investigate nutritional and anti-nutrient content of three Benin’s kola nuts (Cola nitida, Cola acuminata and Garcinia kola). Proximate composition of the three species of kola nuts was assessed using standard analytical AOAC methods. Phenolics and flavonoids contents were determined by Folin-Ciocalteu and aluminum trichloride methods, respectively. Mineral composition was determined by Atomic Absorption Spectrometry method. Free and total amino acids were separated and quantified by HPLC. Protein content of the three kola nuts ranges from 4.95% (G. kola) to 10.64% (C. acuminata) whereas fat content ranges from 0.2 ± 0.00 (C. nitida) to 2.5 ± 0.42 (G. kola). Total phenolics abounded (2444.96 ± 81.56 μg Eq AG/100g) in C. acuminata, while flavonoids predominated (561.69 ± 22.10 μgEqQ/100g) in G. kola. The three species are a good source of magnesium and a copper provider was lowest in C. nitida (0.59 ± 0.08 mg/g) and in C. acuminata (0.65 ± 0.02 mg/g). The dominant total essential amino acids were threonine (C. acuminata) and methionine (C. acuminata and G. kola), while the predominant non-essential total amino acids according to species were arginine (C. nitida and G. kola), proline (C. acuminata) and cysteine (G. kola). For the anti-nutrients factors, saponins were in great proportion (8.33% ± 0.25%), while the oxalates were in small proportion (0.44% ± 0.04%). The three species have an interesting nutritional composition, but these seeds have the relatively lowest amino acids content.
Cite this paper: Dah-Nouvlessounon, D. , Adjanohoun, A. , Sina, H. , Noumavo, P. , Diarrasouba, N. , Parkouda, C. , Madodé, Y. , Dicko, M. and Baba-Moussa, L. (2015) Nutritional and Anti-Nutrient Composition of Three Kola Nuts ( Cola nitida, Cola acuminata and Garcinia kola ) Produced in Benin. Food and Nutrition Sciences, 6, 1395-1407. doi: 10.4236/fns.2015.615145.
References

[1]   Adesuyi, A.O., Elumm, I.K., Adaramola, F.B. and Nwokocha, A.G.M. (2012) Nutritional and Phytochemical Screening of Garcinia kola. Advance Journal of Food Science and Technology, 4, 9-14.

[2]   Okigbo, B.N. (1977) Neglected Plants of Horticultural Importance in Traditional Farming Systems of Tropical Africa. Acta Horticulturae, 53, 131-150.
http://dx.doi.org/10.17660/ActaHortic.1977.53.18

[3]   Getahun, A. (1990) The Role of Wild Plants in the Native Diet in Ethiopia. Agroecosystem, 1, 45-56.

[4]   Falconer, J. (1990) Hungry Season, Food from the Forest. FAO Unasylva, 41, 14-19

[5]   Peters, C.R. (1987) Ricinodendron rautanenii (Euphorbiaceae): Zambezian Wild Food Plant for All Seasons. Economic Botany, 41, 494-502.
http://dx.doi.org/10.1007/BF02908143

[6]   Wehmeyer, A.S. (1966) The Nutrient Composition of Some Edible Wild Fruits found in the Transvaal. South African Medical Association, 40, 1102-1104.

[7]   Lockett, C.T., Calvert, C.C. and Grivetti, L.E. (2000) Energy and Micronutrient Composition of Dietary and Medicinal Wild Plants Consumed during Drought. Study of Rural Fulani, Northeastern Nigeria. International Journal of Food Sciences and Nutrition, 51, 195-208.
http://dx.doi.org/10.1080/09637480050029700

[8]   Nnam, N.M. and Obiakor, P.N. (2003) Effect of Fermentation on the Nutrient and Antinutrient Composition of Baobab (Adansonia digitata) Seeds and Rice (Oryza sativa) Grains. Ecology of Food and Nutrition, 42, 265-277.
http://dx.doi.org/10.1080/03670244.2003.9657684

[9]   Ogutuga, D.B.A. (1975) Chemical Compositon and Potential Commercial Uses of Kolanut. Cola nitida, Vent (Schott and Endicher). Ghana Journal of Agricultural Science, 8, 121-125.

[10]   Okoli, B.J., Abdullahi, K., Myina, O. and Iwu, G. (2012) Caffeine Content of Three Nigerian Cola. Journal of Emerging Trends in Engineering and Applied Sciences, 3, 830-833.

[11]   Lowe, H.I.C., Watson, C.T., Badal, S., Peart, P., Toyang, N.J. and Bryant, J. (2014) Promising Efficacy of the Cola acuminata Plant: A Mini Review. Advances in Biological Chemistry, 4, 240-245.
http://dx.doi.org/10.4236/abc.2014.44029

[12]   Jarvis, G. (2002) The Rise and Fall of Cocaine Cola.
http://www.lewrockwell.com/jarvis/jarvis17.html

[13]   Akoegninou, A., Van Der Burg, W.J. and Van Der Maesen, L.J.G. (2006) Flore analytique du Bénin, Backhuys Publishers, Leiden, Netherlands, Wageningen University Papers 06.2.

[14]   Farombi, E.O., Tahnteng, J.G., Agboola, A.O., Nwankwo, J.O. and Emerole, G.O. (2000) Chemoprevention of 2-Acetylaminofluorene-Induced Hepatotoxicity and Lipid Peroxidation in Rats by Kolaviron—A Garcinia kola Seed Extract. Food and Chemical Toxicology, 38, 535-541.
http://dx.doi.org/10.1016/S0278-6915(00)00039-9

[15]   Pietta, P.G. (2000) Flavonoids as Antioxidants. Journal of Natural Products, 63, 1035-1042.
http://dx.doi.org/10.1021/np9904509

[16]   Okunji, C.O., Ware, T.A., Hicks, R.P., Iwu, M.M. and Skanchy, D.J. (2002) Capillary Electrophoresis Determination of Biflavanones from Garcinia kola in Three Traditional African Medicinal Formulations. Planta Medica, 68, 440-444.
http://dx.doi.org/10.1055/s-2002-32091

[17]   Dah-Nouvlessounon, D., Adoukonou-Sagbadja, H., Diarrassouba, N., Sina, H., Noumavo, P.A., Baba-Moussa, F., Adjanohoun, A., Gbenou, J.D. and Baba-Moussa L. (2015) Antimicrobial, Antioxidant, Cytotoxic Activities and Phytochemical Assessment of Cola acuminata Used in Benin. International Journal of Pharmacy and Pharmaceutical Sciences, 7, 1-8.

[18]   Dewole, E.A., Dewumi, D.F.A., Alabi, J.Y.T. and Adegoke A. (2013) Proximate and Phytochemical of Cola nitida and Cola acuminata. Pakistan Journal of Biological Sciences, 16, 1593-1596.

[19]   Theil, E.C., Burton, J.W. and Beard, J.L. (1997) A Sustainable Solution for Dietary Iron Deficiency through Plant Bio-Technology and Breeding to Increase Seed Ferritin Control. European Journal of Clinical Nutrition, 51, 28-31.

[20]   Kolawole, S.E. and Obueh, H.O. (2013) A Study of the Oxalate, Phytate and Cyanide Contents of Selected Nigerian Foods and Diet in Akwa Ibom and Cross River States of Nigeria. African Journal of Food Science and Technology, 4, 44-47.

[21]   AOAC (1990) Official Methods of Analysis of Association of Official Analytical Chemist. In: Herwitz, W., Ed., Association of Official Analytical Chemistry, Washington DC, 125-126.

[22]   Fox, J.D. and Robyt, J.F. (1991) Miniaturization of Three Carbohydrate Analyses Using a Microplate Reader. Analytical Biochemistry, 195, 93-96.
http://dx.doi.org/10.1016/0003-2697(91)90300-I

[23]   Miller, G.L. (1958) Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Biochemistry, 195, 93-96.

[24]   Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology, 299, 152-178.
http://dx.doi.org/10.1016/S0076-6879(99)99017-1

[25]   Yi, Z.B., Yu, Y., Liang, Y.Z. and Zeng, B. (2007) In Vitro Antioxidant and Antimicrobial Activities of the Extract of Pericarpium Citri Reticulatae of a New Citrus Cultivar and Its Main Flavonoids. LWT-Food Science and Technology, 4, 597-603.

[26]   Pinta, A.A. (1973) Méthodes de référence pour la détermination des éléments minéraux dans les végétaux. Oléagineux, ORSTOM, Bondy, France.

[27]   Bildlingmeyer, B.A., Cohen, S.A. and Tarvin, T.L. (1984) Rapid Analysis of Amino Acids Using Pre-Column Derivatization. Journal of Chromatography, 336, 93-104.
http://dx.doi.org/10.1016/S0378-4347(00)85133-6

[28]   Sombié, P.A.E.D., Hilou, A., Coulibaly, A.Y., Tibiri, A., Kiendrebeogo, M. and Nacoulma. O.G. (2011) Brain Protective and Erythrocytes Hemolysis Inhibition Potentials from Galls of Guiera senegalensis J.F. Gmel (Combretaceae). Journal of Pharmacology and Toxicology, 6, 361-370.
http://dx.doi.org/10.3923/jpt.2011.361.370

[29]   Sathya, V., Bharathidasan, R., Tamil, S., Selvi, N. Rebeccal, S., Ilakkiya, R. and Prabakaran, M. (2013) Quantitative, Qualitative Phytochemical Analysis and in Vitro Antibacterial Activity of Bauhinia tomentosa L. Journal of Natural Product and Plant Resources, 3, 31-36.

[30]   Mir, A.M., Sawhney, S.S. and Jassal, M.M.S. (2013) Qualitative and Quantitative Analysis of Phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmacy and Pharmacology, 2, 1-5.

[31]   Lucas, G.M. and Markakas, M. (1975) Phytic Acid and Other Phosphorus Compounds of Bean (Phaseolus vulgaris). Journal of Agricultural and Food Chemistry, 23, 13-15.
http://dx.doi.org/10.1021/jf60197a016

[32]   Ajai, A.I., Ochigbo, S.S., Jacob, J.O., Ndamitso, M.M. and Abubakar, U. (2012) Proximate and Mineral Compositions of Different Species of Kola Nuts. European Journal of Applied Engineering and Scientific Research, 1, 44-47.

[33]   Odebunmi, E.O., Oluwaniyi, O.O., Awolola, G.V. and Adediji, O.D. (2009) Proximate and Nutritional Composition of Kola Nut (Cola nitida), Bitter Cola (Garcinia kola) and Alligator Pepper (Afromomum melegueta). African Journal of Biotechnology, 8, 308-310.

[34]   Lowor, S.T., Aculey, P.C. and Assuah, M.K. (2010) Analysis of Some Quality Indicators in Cured Cola nitida (Vent). Agriculture and Biology Journal of North America, 1, 1206-1214.

[35]   Guisseppe, R. and Baratta, T.M. (2000) Antioxidant Activity of Selected Essential Oil Components in Two Lipid Model Systems. African Journal of Biotechnology, 69, 167-174.

[36]   Adeyeye, E. and Ayejuyo, O. (1994) Chemical Composition of Cola acuminata and Garcinia kola Seeds Grown in Nigeria. International Journal of Food Sciences and Nutrition, 45, 223-230.
http://dx.doi.org/10.3109/09637489409166162

[37]   Arogba, S.S. (2000) Comparative Analyses of the Moisture Isotherms, Proximate Compositions, Physical and Functional Properties of Dried Cola nitida and Garcinia kola Kernels. Journal of Food Compound Analysis, 13, 139-148.
http://dx.doi.org/10.1006/jfca.1999.0839

[38]   Durand, J.L. (2007) Les effets du déficit hydrique sur la plante: Aspects physiologiques. Fourrages, 190, 181-195.

[39]   Lemaire, G., Charrier, X. and Hébert, Y. (1996) Nitrogen Uptake Capacities of Maize and Sorghum Crops in Different Nitrogen and Water Supply Conditions. Agronomies, 16, 231-246.
http://dx.doi.org/10.1051/agro:19960403

[40]   Voet, D.J., Voet, J.G. and Pratt, C.W. (2008) The Principles of Biochemistry. John Wiley & Sons, Hoboken, 74-219.

[41]   Agbeniyi, S.O. and Ayodele, M.S. (2010) Effect of Storage Moulds on the Nutritional Quality of Kolanuts in Nigeria. Pakistan Journal of Nutrition, 9, 512-515.
http://dx.doi.org/10.3923/pjn.2010.512.515

[42]   Dicko, M.H., H. Gruppen, Voragen, A.G.J. and van Berkel, W.J.H. (2002) Comparison of Content in Phenolic Compounds, Polyphenol Oxidase and Peroxidase in Grains of Fifty Sorghum Varieties from Burkina Faso. Journal of Agricultural and Food Chemistry, 50, 3780-3788.
http://dx.doi.org/10.1021/jf011642o

[43]   Moosmann, B. and Behl, C. (1999) The Antioxidant Neuroprotective Effects of Estrogens and Phenolic Compounds Are Independent from Their Estrogenic. Proceedings of the National Academy of Sciences of the United States of America, 96, 8867-8872.
http://dx.doi.org/10.1073/pnas.96.16.8867

[44]   Parr, A.J. and Bolwell, G.P. (2000) Phenols in the Plant and in Man. The Potential for Possible Nutritional Enhancement of the Diet by Modifying the Phenols Content or Profile. Journal of Science Food and Agriculture, 80, 985-1012.
http://dx.doi.org/10.1002/(SICI)1097-0010(20000515)80:7<985::AID-JSFA572>3.0.CO;2-7

[45]   Santos-Buelga, C. and Scalbert, A. (2000) Proanthocyanidins and Tannin-Like Compounds: Nature, Occurrence, Dietary Intake and Effects on Nutrition and Health. Journal of Science Food and Agriculture, 80, 1094-1117.
http://dx.doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1094::AID-JSFA569>3.0.CO;2-1

[46]   Ferreira, D. and Slade, D. (2002) Oligomeric Proanthocyanidins: Naturally Occurring O-Heterocycles. Natural Product Reports, 19, 517-541.
http://dx.doi.org/10.1039/b008741f

[47]   Terashima, K., Takaya, Y. and Niwa, M. (2002) Powerful Antioxidative Agents Based on Garcinoic Acid from Garcinia kola. Bioorganic & Medicinal Chemistry, 10, 1619-1625.
http://dx.doi.org/10.1016/S0968-0896(01)00428-X

[48]   Middleton, E.J., Kandaswani, C. and Theoharides, T.C. (2000) The Effects of Plant Flavonoids on Mammalian Cells, Implication for Inflammation, Heart Disease and Cancer. Pharmacology, 52, 673-751.

[49]   Ajayi, L.A., Dawodu, F.A. and Oderinde, R.A. (2003) Fatty Acid Composition and Metal Content of Adansonia digitata Seeds and Seed Oil. La Rivisita Italiana Delle Sosteanze Grasse, 80, 41-43.

[50]   Nelson, D.L. and Cox, M.M. (2008) Lehninger Principles of Biochemistry. W. H. Freeman and Company, New York, 342-343.

[51]   Meuunier, N., O’Connor, J.M., Maiani, G., Cashman, K.D., Secker, D.L. and Ferry, M. (2005) Importance of Zinc in the Elderly: The ZENITH Study. European Journal of Clinical Nutrition, 59, S1-S4.
http://dx.doi.org/10.1038/sj.ejcn.1602286

[52]   Frederickson, C.J., Suh, S.W., Silva, D., Frederickson, C.J. and Thompson, R.B. (2000) Importance of Zinc in the Central Nervous System: The Zinc Containing Neuron. Journal of Nutrition, 130, 1471-1483.

[53]   Atanda, O.O., Olutayo, A., Mokwunye, F.C., Oyebanji, A.O. and Adegunwa, M.O. (2011) The Quality of Nigerian Kola Nuts. African Journal of Food Science, 5, 904-909.

[54]   Abbaspour, N., Hurrell, R. and Kelishadi, R. (2014) Review on Iron and Its Importance for Human Health. Journal of Research in Medical Sciences, 19, 164-174.

[55]   McDowell, L.R. (2003) Minerals in Animal and Human Nutrition. Elsevier Science, Amsterdam.

[56]   Hollander, D.I., Nagey, D.A. and Pupkin, M.J. (1987) Magnesium Sulfate and Ritodrine Hydrochloride: A Randomized Comparison. American Journal of Obstetrics and Gynecology, 156, 631-637.
http://dx.doi.org/10.1016/0002-9378(87)90066-4

[57]   Feillet-Coudray, C., Coudray, C., Gueux, E., Mazur, A. and Rayssiquier, Y. (2003) A New in Vitro Blood Load Test Using a Magnesium Stable Isotope for Assessment of Magnesium Status. Journal of Nutrition, 133, 1220-1223.

[58]   Gnaglom, E. (2012) Le baobab et ses effets. Nutra News Science, Nutrition, Prévention et Santé. www.africa1.com/spip.php?article26071

[59]   Eleyinmi, A.F., Bressler, D.C., Amoo, I.A., Sporns, P. and Oshodi, A.A. (2006) Chemical Composition of Bitter Cola (Garcinia kola) Seed and Hulls. Polish Journal of Food and Nutrition Sciences, 56, 395-400.

[60]   Adeyeye, E., Asaolu, S. and Aluko, A. (2007) Amino Acid Composition of Two Masticatory Nuts (Cola acuminata and Garcinia kola) and a Snack Nut (Anacardium occidentale). International Journal of Food Sciences and Nutrition, 58, 241-249.
http://dx.doi.org/10.1080/09637480701486108

[61]   Kumar, R. (1991) Anti-Nutritional Factors, the Potential Risks of Toxicity and Methods to Alleviate Them. In: Speedy, A. and Pugliese, P.-C., Eds., Legume Trees and Other Fodder Trees as Protein Sources for Livestock, FAO Corporate Document Repository, Kuala Lumpur, Malaysia, 145-160.

[62]   Shqueir, A.A., Brown, D.L., Taylor, S.J., Rivkin, I. and Klasing, K.C. (1989) Effects of Solvent Extraction, Heat Treatments and Added Cholesterol on Sesbania sesban Toxicity in Growing Chicks. Animal Feed Science and Technology, 27, 127-135.
http://dx.doi.org/10.1016/0377-8401(89)90137-5

[63]   Johnson, I.T., Gee, J.M., Price, K., Curl, C. and Fenwick, G.R. (1986) Influence of Saponin on Gut Permeability and Active Nutrient Transport in Vitro. Journal of Nutrition, 116, 2270-2277.

[64]   Pant, G., Panwaar, M.S., Negi, D.S. and Rawat, M.S. (1988) Spermicidal Activity and Chemical Analysis of Pentapanax leschenaultii. Medica Planta, 54, 476-477.

[65]   Kumar, R. and Sing, M. (1984) Tannins: Their Adverse Role in Ruminant Nutrition. Journal of Agriculture and Food Chemistry, 32, 447-453.
http://dx.doi.org/10.1021/jf00123a006

[66]   Barry, T.N. (1987) Secondary Compounds of Forages. In: Hacker, J.B. and Ternouth, J.H., Eds., Nutrition of Herbivores, Academic Press, Sydney, 91-120.

[67]   Soetan, K.O. and Oyewole, O.E. (2009) The Need for Adequate Processing to Reduce the Antinutritional Factors in Plants Used as Human Foods and Animal Feeds: A Review. African Journal of Food Science, 3, 223-232.

 
 
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