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 OJVM  Vol.5 No.11 , November 2015
Detection of Verocytotoxigenic Escherichia coli O157 Serotype in Dairy Products in Abuja, Nigeria
S. I. Enem1*, S. I. Oboegbulem2, W. D. Nafarnda1, G. K. Omeiza1
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Abstract: Ruminants are recognized as healthy carriers of Verocytotoxigenic Escherichia Coli (VTEC) organisms and as such most dairy products may provide these bacteria with favourable conditions for their growth. A cross sectional study was conducted to detect the occurrence of VTEC O157 in dairy products in the Federal Capital Territory, Abuja, Nigeria. Raw milk, Nunu and yoghurt were analyzed using standard cultural and biochemical procedures to isolate typical E. coli. Isolated E. coli samples were sub-cultured into plates of sorbitol MacConkey and Cefixime Tellurite-Sorbitol MacConkey agar. Isolates that are sorbitol negative were further characterized using commercially procured latex agglutination test kits. A total of 367 samples were analyzed out of which 3 tested positive for VTEC O157 (108 of raw milk-1 (0.93%); 127 of Nunu-2 (1.57%) and 132 of yoghurt-none). There was no significant association (p > 0.05) between season and infection with VTEC O157 in dairy products. Dairy products remained a potential vehicle for VTEC O157 infection.
Keywords: Detection, Prevalence, VTEC O157, Dairy Products
Cite this paper: Enem, S. , Oboegbulem, S. , Nafarnda, W. and Omeiza, G. (2015) Detection of Verocytotoxigenic Escherichia coli O157 Serotype in Dairy Products in Abuja, Nigeria. Open Journal of Veterinary Medicine, 5, 224-228. doi: 10.4236/ojvm.2015.511030.
References

[1]   Chapman, P.A., Siddons, C.A., Cerdan-Malo, A.T. and Harkin, M.A. (1997) A 1-Year Study of Escherichia coli O157 in Cattle, Sheep, Pigs and Poultry. Epidemiology & Infection, 119, 245-250.
http://dx.doi.org/10.1017/S0950268897007826

[2]   Abongo, B.O. and Momba, M.N.B. (2009) Prevalence and Characterization of Escherichia coli O157: H7 Isolates from Meat and Meat Products Sold in Amathole District, Eastern Cape Province of South Africa. International Journal of Food Microbiology, 26, 173-176.
http://dx.doi.org/10.1016/j.fm.2008.10.001

[3]   Centers for Disease Control (1982) Isolation of E. coli O157:H7 from Sporadic Cases of Hemorrhagic Colitis-United States. Morbidity and Mortality Weekly Report, 31, 580-585.

[4]   Kumar, A., Taneja, N., Bharti, B. and Sharma, M. (2014) Characterization of Shiga Toxigenic Escherichia Coli Isolated from Cases of Diarrhea & Haemolytic Uraemic Syndrome in North India. India journal of Medical Research, 140, 778-784.

[5]   Neher, S., Hazarika, A.K., Sharma, R.K., Barkalita, L.M., Bora, M. and Deka, P. (2015) Detection of Shiga-Toxigenic Escherichia Coli in Milk Samples of Cattle by PCR. JAVS, 8, 75-78.

[6]   OIE Terrestrial Manual (2008) Verocytotoxigenic Escherichia Coli
http//www.oient/eng/normes/mannual/2008/pdf/2.09/11vero-E-coli=pat

[7]   Sang, W.K., Boga, H.I., Waiyaki, P.G., Schnasel, D., Wamac, N.C. and Kariuki, S.M. (2012) Prevalence and Genetic Characteristics of Shigatoxigenic Escherichia coli from Patients with Diarrhoea in Maasailand Kenya. Journal of Infection in Developing Countries, 6, 102-108.
http://dx.doi.org/10.3855/jidc.1750

[8]   Perera, A., Clarke, C.M., Dykes, G.A. and Fegan, N. (2015) Characterization of Shiga Toxigenic Escherichia coli O157 and Non O157 Isolates from Ruminant Feces in Malaysia. Biomed Research International, 3, 1-8.
http://dx.doi.org/10.1155/2015/382403

[9]   Borczyk, A.A., Karmali, M.A., Lior, H. and Duncan, L.M.C. (1987) Bovine Reservoiur for Verotoxin—Producing Escherichia coli O157:H7. The Lancet, 239, 98.
http://dx.doi.org/10.1016/S0140-6736(87)91928-3

[10]   Oksüz, O., Arici, M., Kurultay, S. and Gumus, T. (2004) Incidence of Escherichia coli O157 in Raw Milk and White Pickled Cheese Manufactured from Raw Milk in Turkey. Food Control, 15, 453-456.
http://dx.doi.org/10.1016/S0956-7135(03)00121-X

[11]   Le Saux, X.N., Spika, J.S. and Friesen, B. (1993) Ground Beef Consumption in Non Commercial Settings Is a Risk Factor for Spread of Escherichia coli 0157:H7 Infection in Canada. The Journal of Infectious Diseases, 167, 500-502.
http://dx.doi.org/10.1093/infdis/167.2.500

[12]   Blanco, J., Blanco, M., Blance, J.E., Mora, A., Alonso, M.P., Ganza’lez, E.A. and Berna’rdez, M.I. (2001) Epidemiology of Verocytotoxigenic Escherichia coli (VTEC) in Ruminants. In: Dufty, G., Garvey, P. and McDowel, D., Eds., Verocytotoxigenic Escherichia coli, Food and Nutrition Press Inc., Trumbull, 113-148.

[13]   Buetin, L., Geier, D., Steinruch, H., Zimmermann, S. and Scheutz, F. (1993) Prevalence and Some Properties of Verotoxim (Sluga-Like Toxin) Producing Escherichia coli in Seven Different Species of Healthy Domestic Animals. Journal of Clinical Microbiology, 31, 2483-2488.

[14]   Nataro, J.P. and Kaper, J.B. (1998) Diarrhogenic Escherichia coli. Clinical Microbiology Reviews, 11, 142-201.

[15]   Baazize-Ammi, D., Gassem, O., Derrar, F., Izri, K., Brahim-Errahmani, M., Gagnon, J., Guetarni, D. and Chebloune, Y. (2015) Prevalence of Asymptomatic Carriers of Shiga Toxin-Producing Escherichia coli (STEC) in Dairy Cattle Farms in the Governorate of Blida (Algena). Bulletin of the Veterinary Institute in Pulawy, 59, 23-28.
http://dx.doi.org/10.1515/bvip-2015-0004

[16]   Trevisani, M., Mancusi, R., Delle Donne, G., Bacci, C, Bassi, L. and Bonardi, S. (2014) Detection of Shiga Toxin (Stx)-Producing Escherichia coli (STEC) in Bovine Dairy Herds in Northern Italy. International Journal of Food Microbiology, 184, 45-49.
http://dx.doi.org/10.1016/j.ijfoodmicro.2013.12.033

[17]   Kalmus, P., Kramarenko, T., Roasto, M., Meremac, K. and Viltrop, A. (2015) Quality of Raw Milk Intended for Direct Consumption in Estonia. Food Control, 51, 135-139.
http://dx.doi.org/10.1016/j.foodcont.2014.11.018

[18]   Dawan, P.A. (2000) Geography of Abuja Federal Capital Territory. Famous and Ansala, Minna.

[19]   Edwards, P.R. and Ewing, W.H. (1986) Identification of Enterobacteriaceal. 4th Edition, Burges Publishing Company, Minneapolis.

[20]   Kiranmayi, C.H. and Krishnaiah, N. (2011) Detection of Shiga-Toxigenic Escherichia coli by PCR. Indian Veterinary Journal, 88, 19-20.

[21]   Roopnarine, R.R., Ammons, D., Rampersad, J. and Adesiyun, A.A. (2007) Occurrence and Characterization of Verocytotoxigenic Escherichia coli (VTEC) Strains from Dairy Farms in Trimdad. Zoonoses and Public Health, 54, 78-85.
http://dx.doi.org/10.1111/j.1863-2378.2007.01024.x

[22]   Dorn, C.R. (1995) Escherichia coli 0157:H7. Zoonosis Update.

[23]   Morgan, D., Newman, C.P., Hutchison, D.N., Walker, A.M., Rowe, B. and Majid, F. (1993) Verotoxin Producing Escherichia coli O 157 Infection Associated with Consumption of Yoghurt. Epidemiology and Infection, 111, 181-188.
http://dx.doi.org/10.1017/S0950268800056880

[24]   Chapman, P.A., Wright, D.J. and Higginus, R. (1993) Untreated Milk as a Source of Verocytotoxigenic Escherichia coli 0157. Veterinary Record, 137, 314-315.

[25]   CDSC (1998) VTEC 0157 Infection and Unpasteurized Cream Update. Communicable Disease Report. CDR Weekly, 8, 389-392.

 
 
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