OJCD  Vol.5 No.4 , December 2015
Cytogenetic Effects of Low-Dose Tritiated Water in Human Peripheral Blood Lymphocytes—Experimental Studies on the Relative Biological Effectiveness and Chromosome Aberration Rate and CBMN in Human Blood Lymphocyte Irradiated by Tritium Low Dose Tritium β-Rays and 60Co γ-Rays
Abstract: In this study, the yields of unstable chromosome aberrations in human lymphocytes induced by β particles from low-dose HTO have been measured. HTO was mixed with heparinized blood in varying amounts so that a dose of 6.24 × 10-4 Gy to 1.23 Gy were delivered in 24 and 48 h, respectively. After 72-h culture, the dicentric yield was measured as a function of dose to the blood and compared with data from 60Co γ-radiation. Using a linear-quadratic dose-effect relation to fit the experimental data, a significant linear contribution Y = 0.062D + 0.053D2 was found. The main difference between the coefficients for β and γ-radiation was in the a values, indicating that HTO β-rays were more efficient, particularly at low doses. As per the theory of dual radiation, the relative biological effectiveness (RBE) of HTO β-particles relative to γ-rays is 2.21 at 0.06 Gy and decreases with increasing dose. Micronucleus yield at low doses was fitted to a linear equation Y = C + αD, indicating that the RBE value of MN for HTO β-rays irradiation was between 1.46 and 2.17, which is similar in shape to the chromosome aberrations experiments. Thus, β-rays were found to be more efficient in producing two lesions with single ionizing tracks at low dose.
Cite this paper: Deng, B. , Hou, J. , Quan, Y. , Dong, L. and Tan, Z. (2015) Cytogenetic Effects of Low-Dose Tritiated Water in Human Peripheral Blood Lymphocytes—Experimental Studies on the Relative Biological Effectiveness and Chromosome Aberration Rate and CBMN in Human Blood Lymphocyte Irradiated by Tritium Low Dose Tritium β-Rays and 60Co γ-Rays. Open Journal of Clinical Diagnostics, 5, 125-135. doi: 10.4236/ojcd.2015.54021.

[1]   Filer, C.N. (2009) Tritium Labelled Photoaffinity Agents. Radioanalytical and Nuclear Chemistry, 281, 521-530.

[2]   Dassi, L. (2010) Use of Chloride Mass Balance and Tritium Data for Estimation of Groundwater Recharge and Renewal Rata in an Unconfined Aquifer from North Africa: Case Study from Tunisia. Environ Earth, 60, 861-871.

[3]   IAEA (1996) International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources IAEA Safety. Series No. 115, Vienna.

[4]   Galeriu, D., Melintescu, A. and Takeda, H. (2007) Risk from Tritium Exposure, IRPA. Regional Congress for Central and Eastern Europe, 9, 24-28.

[5]   Fenech, M. (2010) The Lymphocyte Cytokinesis-Block Micronucleus Cytomeassay and Its Application in Radiation Biodosimetry. Health Physics, 26, 11-17.

[6]   Samavat, H. and Mozdarani, H. (2004) Chromosomal Abrrations in Iranian Radiation Workers Due to Chronic Exposure of X-Irradiation. International Journal of Low Radiation, 1, 216-222.

[7]   Fenech, M. (2007) Cytokinesis-Block Micronucleus Cytome Assay. Nature Protocols, 2, 1084-1104.

[8]   Thomas, P. and Fenech, M. (2011) Cytokinesis-Block Micronucleus Cytome Assay in Lymphocytes. Methods in Molecular Biology, 682, 217-234.

[9]   Bocian, E., Ziemb_Zak, B., Rosiek, O., et al. (1977) The Use of Nitroaromatic Compounds as Hypoxic Cell Radiosensitizers. Current Topics in Radiation Research Quarterly, 8, 347-398.

[10]   Prosser, J.S., Lloyd, D.C., Edwards, A.A., et al. (1984) Biological Dosimetry in Radiological Protection: Recent Developments. Journal of the Society for Radiological Protection, 4, 21.

[11]   Bender, M.L. (1962) Molecular Biochemistry. Science, 138, 672-673.

[12]   Dobson, R.L. (1986) Health Effects of Low Level Radiation: Carcinogenesis, Teratogenesis, and Mutagenesis Effects of Low Level Radiation. Seminars in Nuclear Medicine, 16, 106-117.

[13]   IAEA (1986) Biological Dosimetry: Chromosomal Aberration Analysis for Dose Assessment. Technical Report Series No. 26. IAEA, Vienna.

[14]   Sreedevi, B., Shankaanaayanan, N. and Rao, B.S. (1997) The Induction of Micronuclei and Chromosomal Aberrations in Human Lymphocytes Irradiated in Vitro with Fission Neutrons. Radiation Protection and Environment, 20, 21-25.

[15]   Balonov, M.I., Likhtarev, I.A. and Moskalev, I.Y. (1984) The Metabolism of 3H Compounds and Limits for Intakes by Workers. Health Physics, 47, 761-773.

[16]   Svensson, H., Hanson, G.P. and Zsdanzky, K. (1990) The IAEA/WHO TL Dosimetry Service for Radiotherapy Centres 1969-1987. Acta Oncologica, 29, 461-467.

[17]   Tawn, E.J. and Thuerens, H. (2009) Dose Response Relationships for Chromosome Aberrations Induced by Low Doses of Alpha-Particle Radiation. Radiation Protection Dosimetry, 135, 268-271.

[18]   Fedorenko, B.S. (2006) Radiobiological Effects of Corpuscular Radiations: Radiation Safety of Space Flights. Nauka, Moscow. (In Russian)

[19]   Tanaka, K., Sawada, S. and Kamada, N. (1994) Relative Biological Effectiveness and Dose Rate Effect of Tritiated Water on Chromosomes in Human Lymphocytes and Bone Marrow Cells. Mutation Research Letters, 323, 53-61.

[20]   Johnson, J.R., Myers, D.K., Jackson, J.S., Dunford, D.W., Gragtmans, N.J., Wyatt, H.M., et al. (1995) Relative Biological Effectiveness of Tritium for Induction of Myeloid Leukemia in CBA/H Mice. Radiation Research, 144, 82-89.

[21]   Kozlowski, R., Bouffler, S.D., Haines, J.W., Harrison, J.D. and Cox, R. (2001) In Utero Haemopoietic Sensitivity to Alpha, Beta or X-Irradiation in CBA/H Mice. International Journal of Radiation Biology, 77, 805-815.

[22]   Ueno, A.M., Furuno-Fukushi, I. and Matsudaira, H. (1982) Induction of Cell Killing, Micronuclei, and Mutation to 6-Thioguanine Resistance after Exposure to Low-Dose-Rate γ Rays and Tritiated Water in Cultured Mammalian Cells (L5178Y). Radiation Research, 91, 447-456.

[23]   Yamada, T., Yukawa, O., Asami, K. and Nakazawa, T. (1982) Effect of Chronic HTO β or 60Co γ Radiation on Preimplantation Mouse Development in Vitro. Radiation Research, 92, 359-369.

[24]   IAEA (2001) Cytogenetic Analysis for Radiation Dose Assessment. Technical Report Series No. 405. IAEA, Vienna.

[25]   Satow, Y., Hori, H. and Lee, J.-Y. (1989) Teratogenic Effect of Fission Neutron and Tritium Water on Rat Embryo. Journal of UOEH, 11, 416-431.

[26]   Revina, V.S., Voronin, V.S., Lemberg, V.K. and Sukhodoev, V.V. (1984) Comparative Evaluation of the Tumorigenic Effect of Chronic Exposure to Tritium Oxide and External γ Radiation. Radiobiologiia, 24, 697-700.

[27]   Chopra, C. and Heddle, J.A. (1988) Cytogenetic Measurements of the Relative Biological Effectiveness of Tritium. Report INFO-0287. Atomic Energy Control Board, Ottowa.

[28]   Vulpis, N. (1984) The Induction of Chromosome Aberrations in Human Lymphocytes by in Vitro Irradiation with β Particles from Tritiated Water. Radiation Research, 97, 511-518.

[29]   IAEA (2000) The Radiological Accident in Istanbul. IAEA, Vienna.

[30]   Schreiber, G.A. (1992) An Automated Flow Cytometric Micronucleus Assay for Human Lymphocytes. International Journal of Radiation Biology, 62, 695-709.

[31]   Tates, A.D., Van Welie, M.J. and Ploen, J.S. (1990) The Present Status of Automated Micronucleus Test for Lymphocytes. International Journal of Radiation Biology, 58, 813-825.