Armel Laibi¹, Marcel Senou², Etienne Sagbo^1*, Mohamed Dahouda², Virgil Ahyi³, Gado Tchangbedji⁴

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¹ Faculté des Sciences et Techniques, Campus Universitaire d’Abomey-Calavi, Cotonou, Bénin.
² Faculté des Sciences Agronomiques, Campus Universitaire d’Abomey-Calavi, Cotonou, Bénin.
³ Institut Régional du Génie Industriel des Biotechnologies, IRGIB Africa, Cotonou, Bénin.
⁴ Faculté des Sciences, Université de Lomé, Lomé, Togo.
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Abstract: Four groups each composed of six rams with 15 kg average body weight were submitted to one of the following diet: Panicum maximum C1 (PM), Panicum maximum C1 plus concentrate (PM_Co), Panicum m. C1 plus clay (PM_Ar ) and Panicum maximum C1 plus concentrate plus clay (PM_Co_Ar). The concentrate is composed of 90% weat bran, 9.25% cottonseed cake, 0.5% premix and 0.25 cooking salt. The clay used was a mixture of 59% smectite, 14% kaolinite and 27% quartz. The significance of treatments on variation in physico-chemical characteristics (ruminal pH and concentration of ammonia nitrogen), as well as in the rumen fermentation profile i.e. Volatile Fatty Acid concentration and in the enteric methane (CH₄) production, depending on the rumen fluid collection time (at 9 o’clock and 13 o’clock), were studied using AOV. The highest ruminal pH was recorded in animals fed the mixture of Panicum plus clay, which is 7.02 ± 0.12 against 6.96 ± 0.12, 6.8 ± 0.06 and 6.69 ± 0.15, respectively in animals fed with PM, PM_Co and PM_Co_Ar. Incorporation of clay in the mixture of Panicum C1 plus concentrate had a stabilizing effect on ruminal pH. A significant decrease in the concentration of ruminal N-NH3 was recorded with PM_Ar (56.6 ± 15.2 g/ml), compared to PM (89.0 ± 18.9 g/ml). However, the ruminal N-NH₃ concentration of animals fed the PM_Co diet (71.0 ± 15.1g /ml) increased in comparison to that of the PM_Co_Ar diet fed animals. Compared to the enteric methane concentration of the PM ration fed animals, the decrease of CH4 concentration was 2% - 5%, 7% - 11% and 19% - 23% respectively in the PM_Ar, the PM_Co and the PM_Co_Ar diets fed rams. These results suggest that the methane reducing effect of clay might be emphasized by the concentrate supplementation of the diet.

Keywords: Greenhouse Gases, Djallonké Rams, Methanogenesis, Clay Supplementation

Cite this paper: Laibi, A. , Senou, M. , Sagbo, E. , Dahouda, M. , Ahyi, V. and Tchangbedji, G. (2015) The Use of Clay as a Methane Emission Mitigating Feed Additive in Djallonké Rams. Journal of Environmental Protection, 6, 1176-1185. doi: 10.4236/jep.2015.610105.

References

[1]   Ouachem, D., Soltane, M. and Kalli, A. (2008) Les pailles de céréales: Profil des fermentations et production de méthane. Sciences & Technologie, 27, 23-28.

[2]   Chenoweth, D.P. (1996) Environmental Impact of Methanogenesis. Environmental Monitoring and Assessment, 42, 3-18.
http://dx.doi.org/10.1007/BF00394039

[3]   Jouany, J.-P. and Thivend, P. (2008) La production de méthane d’origine digestive chez les ruminants et son impact sur le réchauffement climatique. Management & Avenir, 6, 259-274.
http://dx.doi.org/10.3917/mav.020.0259

[4]   CITEPA (2010) Inventaire des émissions de polluants atmosphériques en France. Séries sectorielles et analyses étendues. Format SECTEN, Rapport National d’Inventaire, 316.

[5]   Ouachem, D. and Ghamri, N. (2002) Effets de l’argile sur la digestibilité du foin et la croissance chez les Ovins. Revue Sciences Agronomiques et Forestières, 1.

[6]   Laibi, B.A. (2008) Caractérisation physicochimique et minéralogique de quelques échantillons d’argile du bassin sédimentaire côtier du Bénin. Mémoire de DEA, Université d’Abomey Calavi, 80.

[7]   Jaroslav, F. and Dvorak, R. (2009) Determination of Volatile Fatty Acid Content in the Rumen Liquid: Comparison of Gas Chromatographiy and Capillary Isotachophoresis. Acta Veterinaria Brno, 78, 627-633.
http://dx.doi.org/10.2754/avb200978040627

[8]   Demeyer, D.I. (1991) Quantitative Aspects of Microbial Metabolism in the Rumen and Hindgut. In: Jouany. J.P., Ed., Rumen Microbial Metabolism and Ruminant Digestion, Editions INRA Paris, 217-237.

[9]   Erfle, J.D., Boila, R.J., Teather, R.M., Mahadevan, S. and Sauer, F.D. (1982) Effect of pH on Fermentation Characteristics and Protein Degradation by Rumen Micro-Organisms in Vitro. Journal of Dairy Science, 65, 1457-1464.
http://dx.doi.org/10.3168/jds.S0022-0302(82)82368-0

[10]   Fonty, G., Jouany, J.P., Forano, E. and Gouet, P.H. (1995) Nutrition des ruminants domestiques: L’écosystème microbien du réticulo-rumen. Editions INRA, 299-348.

[11]   De Smith, S. and Demeyer, D.I. (1992) Dégradabilité in sacco: Variabilité entre animaux. Annales de Zootechnie, 41, 21-22.
http://dx.doi.org/10.1051/animres:19920107

[12]   Philippeau, C., Landry, J. and Michalet-Doreau, B. (2000) Influence of the Protein Distribution of Maize Endosperm on Ruminal Starch Degradability. Journal of the Science of Food and Agriculture, 80, 404-408.
http://dx.doi.org/10.1002/1097-0010(200002)80:3<404::AID-JSFA541>3.0.CO;2-Z

[13]   Offner, A., Chapoutot, P. and Sauvant, D. (2002) Comparaison de trois modèles du rumen sur leur aptitude à prédire la digestion des glucides pariétaux et amylacés. 9ème Rencontre Recherche Ruminants, Paris, 4-5 Décembre 2002, 336.

[14]   Ben Ahmed, H. and Dulphy, J.P. (1987) Influence de la complémentation des foins traités à l’ammoniac sur la valeur nutritive. Annales de Zootechnie, 36, 153-170.
http://dx.doi.org/10.1051/animres:19870205

[15]   Dulphy, J.P., Jamot, J., Chenost, M., Besle, J.M. and Chiofalo, V. (1992) The Influence of Urea Treatment on the Intake of Wheat Straw in Sheep. Annales de Zootechnie, 41, 169-185.
http://dx.doi.org/10.1051/animres:19920205

[16]   INRA (1978) Alimentation des ruminants. INRA Publication, Versailles, 597 p.

[17]   Sauvant, D. and Peyraud, J.L. (2010) Calculs de ration et évaluation du risque d’acidose. Inra Production Animale, 23, 333-342.

[18]   Sauvant, D., Giger-Reverdin, S. and Serment Broudiscou, L. (2011) Influences des régimes et de leur fermentation dans le rumen sur la production de méthane par les ruminants. INRA Production Animale, 24, 433-446.

[19]   Leng, R.A., Inthapanya, S. and Preston, T.R. (2012) Biochar Lowers Net Methane Production from Rumen Fluid in Vitro. Livestock Research for Rural Development, 24, Article #103.
http://www.lrrd.org/lrrd246/sang24103.htm