JEP  Vol.6 No.10 , October 2015
Detection of Enterobacter sakazakii from Commercial Children Dry Milk
Abstract: This study included isolation and identification of E. sakazakii from 51 different samples of powdered infant formula milk involved (Dialak 1 & 2, Celia 1 & 2, Primalac, Biomil 1 & 2, Similac, Nictalia 1 & 2, MAMi, Novolac (AD), Novolac (AR), Novolac (Allernova), S-26 AR, Nursoy, S-26 PDF gold. The results showed that one batch from three of the batches identified of Novolac and Dialak were contaminated and all the types of other infants were non-contaminated. The strains code given as (E1, E2, E3, E4); the bacteria showed resistance to antibiotics used was cephalosporin batch; the third generation showed sensitive to antibiotics life results through inhibition processes such as (Cefotaxime, Sifutetan and Siftadizim), where the diameters of inhibition zone for Siftadizim (18 mm), Sifutetan (22 mm) & Cefotaxime (25 mm) confirmed the bacteria by API and Vitek Compact- 2 (biomero).
Cite this paper: Jaffaar, M. , Shebli, M. , Mussa, A. , Hadi, B. , Aenab, A. and Singh, S. (2015) Detection of Enterobacter sakazakii from Commercial Children Dry Milk. Journal of Environmental Protection, 6, 1170-1175. doi: 10.4236/jep.2015.610104.

[1]   Forsythe, S. and Iversen, C. (2008) Isolation of Enterobacter sakazakii and Other Enterobacteriaceae from Powdered Infant Formula Milk and Related Products. Food Microbiology, 21, 771-773.

[2]   Lecour, H., Seara, A., Cordeiro, J. and Miranda, M. (1989) Treatment of Childhood Bacterial Meningitis. Infection, 17, 343-346.

[3]   Van Acker, J., DeSmet, F., Muyldermans, G., Bougatef, A., Naessens, A. and Lauwers, S. (2001) Outbreak of Necrotizing Enterocolitis Associated with Enterobacter sakazakii in Powdered Milk Formula. Journal of Clinical Microbiology, 39, 293-297.

[4]   Kandhai, M., Reij, W., Gorris, L. and Guillaume, O. (2009) Occurrence of Enterobacter sakazakii in Food Production Environments and Households. Lancet, 363-369.

[5]   Muytjens, H.L. and van der Ros-van de Repe, J. (1986) Comparative in Vitro Susceptibilities of Eight Enterobacter Species, with Special Reference to Enterobacter sakazakii. Antimicrobial Agents and Chemotherapy, 29, 367-370.

[6]   Hoekstra, R.M., Kuehnert, M. and McDonald, L.C. (2007) Invasive Enterobacter sakazakii Disease in Infants. National Center for Infections Disease, 5, 885-895.

[7]   FDA (2002) Isoilation and Enumeration of Enterobacter sakazakii from Dehydrated Infant Formula.

[8]   Iversen, C., Druggan, P. and Forsythe, S.J. (2004) A Selective Differential Medium for Enterobacter sakazakii. International Journal of Food Microbiology, 96, 133-139.

[9]   (2002) Performance Standards for Antimicrobial Disk Susceptibility Testing. NCCLSS, Wayne.

[10]   Muytjens, H. (1985) Enterobacter sakazakii: Identification and Clinical Significance. Antonie van Leeuwenhoek, 51, 618.

[11]   Iversen, C., Druggan, P. and Forsythe, S. (2004) A Selective Medium for Enterobacter sakazakii, a Preliminary Study. International Journal of Food Microbiology, 96, 133-139.

[12]   FAO (1994) Codex Alimenterius Code of Hygienic Practice for Foods for Infants and Children. CAC/RCP 21-1979. Food and Agriculture Organization of the United Nations, Rome.

[13]   Pagotto, F. (2009) Pathogenesis of Cronobacter Enterotoxin Production, Adherence and Invasion of the Blood-Brain Barrier, Burenu of Microbiol Hazards, Ireland.

[14]   Joshua, B., Jeffery, L., Kornacki, B. and Larry, R. (2005) Enterobacter sakazakii: Acoliform of Increased Concern to Infant Heath. Center for Food Safety and Technology, Georgia.

[15]   Naqvi, S.H., Maxwell, M.A. and Dunkle, L.M. (1985) Cefotaxime Therapy of Neonatal Gram-Negative Bacillary Meningitis. The Pediatric Infectious Disease Journal, 4, 499-502.

[16]   Lai, K.K. (2001) Enterobacter sakazakii Infections among Neonates, Infants, Children, and Adults. Case Reports and a Review of the Literature. Medicine, 80, 113-122.