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 OJAS  Vol.5 No.4 , October 2015
PrP Gene Polymorphism in Medieval Remains of Sicilian Sheep
Abstract: Encephalopathy in sheep was at first described in Ireland in 1732 and was called scrapie. Ancient DNA in archaeogenetics represents an effective method to evaluate the ancestral pedigree of living animals and track evolutionary changes occurred between the past and the present day. Since several point mutations are today widely described in modern scrapie, no data about both sequence and frequency are still available for the prion protein (PrP) gene in ancient breeds. In order to evaluate whether the haplotypes distribution in ancient sheep differed from those of the modern population we evaluated polymorphism at four well know codons of the Prp Open Reading Frame. In the present work, we collected 37 medieval sheep bone remains found at the Calathamet (n = 11), Palazzo Bonagia (n = 12) and Palazzo Steri (n = 14) Sicilians archeological sites and dated back between 9th - 15th century. Single nucleotide polymorphisms (SNPs) at codons 136, 141, 154 and 171 of the prion protein (PrP) were investigated using cycle sequencing. Sequenom Mass ARRAYiPLEX platform confirmed the results for 5 individuals out of 37. Cycle sequencing showed at all samples the AA136LL141RR154QQ171 (hereafter ALRQ/ALRQ) genotype except at 2 individuals showing the very susceptible genotype VLRQ/VLRQ (n = 1) and the resistant (ALRR/ ALRR) (n = 1) respectively. Supported by a high incidence of susceptible genotype to prion infection we concluded that presumably scrapie was already widespread enough in the medieval Sicily. Moreover, we described conceivable scenarios that could have underlain evolutionary changes in the medieval sheep population.
Cite this paper: Reale, S. , Cosenza, M. , Sìneo, L. , Arcoleo, L. , Zaffora, G. , Macrì, D. and Vitale, F. (2015) PrP Gene Polymorphism in Medieval Remains of Sicilian Sheep. Open Journal of Animal Sciences, 5, 486-494. doi: 10.4236/ojas.2015.54050.
References

[1]   Prusiner, S.B. (1991) Molecular Biology of Prion Diseases. Science, 252, 1515-1522.
http://dx.doi.org/10.1126/science.1675487

[2]   Leopoldt, J.G. (1750) Nutzliche und auf die Erfahrung gegrundete Einleitung zu der Land-Wirthschafft, 5, Johann Gottlieb Rothen, Sorau.

[3]   Schneider, K., Fangerau, H., Michaelsen, B. and Raab, W.H.M. (2008) The Early History of the Transmissible Spongiform Encephalopathies Exemplified by Scrapie. Brain ResearchBulletin, 77, 343-355.
http://dx.doi.org/10.1016/j.brainresbull.2008.09.012

[4]   Cravero, G., Guarda, F., Dotta, U. and Guglielmino, R. (1976) La scrapie in pecore di razza biellese. Prima segnalazione in Italia. La Clinica Veterinaria, 100, 1-14.

[5]   Bossers, A., Belt, P.B.G.M., Raymond, G.J., Caughey, B., de Vries, R. and Smits, M.A. (1997) Scrapie Susceptibility- Linked Polymorphismsmodulate the in Vitro Conversion of Sheep Prion Protein to Protease Resistantforms. Proceedings of the National Academy of Sciences, USA, 94, 4931-4936.
http://dx.doi.org/10.1073/pnas.94.10.4931

[6]   Heaton, M.P., Leymaster, K.A., Freking, B.A, Hawk, D.A., Smith, T.P., Keele, J.W., Senlling, W.M., Fox, J.M., Chitko-McKown, C.G. and Laegreid, W.W. (2003) Prion Gene Sequence Variation within Diverse Groups of U.S. Sheep, Beef Cattle, and Deer. Mammalian Genome, 14, 765-777.
http://dx.doi.org/10.1007/s00335-003-2283-y

[7]   Goldmann, W., Hunter, N., Smith, G., Foster, J. and Hope, J. (1994) PrP Genotype and Agent Effects in Scrapie: change in Allelic Interaction with Different Isolates of Agent in Sheep, a Natural Host of Scrapie. Journal of General Virology, 75, 989-995.
http://dx.doi.org/10.1099/0022-1317-75-5-989

[8]   Clouscard, C., Beaudry, P., Elsen, J.M., Milan, D., Dussaucy M., Bounneau C., Schelcher, F., Chatelain, J., Launay, J. M. and Laplanche, J.L. (1995) Different Allelic Effects of the Codons 136 and 171 of the Prion Protein Gene in Sheep with Natural Scrapie. Journal of General Virology, 76, 2097-2101. http://dx.doi.org/10.1099/0022-1317-76-8-2097

[9]   Hunter, N., Goldmann, W., Smith, G. and Hope, J. (1994) The Association of a Codon 136 PrP Gene Variant with the Occurrence of Natural Scrapie. Archives of Virology, 137, 171-177.
http://dx.doi.org/10.1007/BF01311184

[10]   Baylis, M., Goldmann, W., Houston, F., Cairns, D., Chong, A., Ross, A., Smith, A., Hunter, N. and McLean, A.R. (2002) Scrapie Epidemic in a Fully PrP-Genotyped Sheep Flock. Journal of General Virology, 83, 2907-2914.
http://dx.doi.org/10.1099/0022-1317-83-11-2907

[11]   Thorgeirsdottir, S., Sigurdarson, S., Thorisson, H.M., Georgsson, G. and Palsdottir, A. (1999) PrP Gene Polymorphism and Natural Scrapie in Icelandic Sheep. Journal of General Virology, 80, 2527-2534.
http://dx.doi.org/10.1099/0022-1317-80-9-2527

[12]   Belt, P.B., Muileman, I.H., Schreuder, B.E., Bos-de Ruijter, J., Gielkens, A.L. and Smits, M.A. (1995) Identification of Five Allelic Variants of the Sheep PrP Gene and Their Association with Natural Scrapie. Journal of General Virology, 76, 509-517.
http://dx.doi.org/10.1099/0022-1317-76-3-509

[13]   Hunter, N., Foster, J.D., Goldmann, W., Stear, M.J., Hope, J. and Bostock, C. (1996) Natural Scrapie in a Closed Flock of Cheviot Sheep Occurs Only in Specific PrP Genotypes. Archives of Virology, 141, 809-824.
http://dx.doi.org/10.1007/BF01718157

[14]   Baylis, M. and Goldmann, W. (2004) The Genetics of Scrapie in Sheep and Goats. Current Molecular Medicine, 4, 385-396.
http://dx.doi.org/10.2174/1566524043360672

[15]   Dawson, M., Hoinville, L.J., Hosie, B.D. and Hunter, N. (1998) Guidance on the Use of PrP Genotyping as an Aid to the Control of Clinical Scrapie. Veterinary Record, 142, 623-625.

[16]   Pongolini, S., Bergamini, F., Iori A., Migliore, S., Corradi, A. and Bassi, S. (2009) Prion Protein Genotypes of Italian Sheep Breeds with Lysine-171 and Phenylalanine-141 Detection. Veterinary Microbiology, 137, 18-23.
http://dx.doi.org/10.1016/j.vetmic.2008.12.012

[17]   Van Kaam, J.B.C.H.M., Finocchiaro, R., Vitale, M., Pinelli, F., Scimonelli, M., Vitale, F., Portolano, B., Oltenacu, P.A. and Caracappa, S., (2008) Prion Protein Gene Frequencies in Three Sicilian Dairy Sheep Populations. Italian Journal of Animal Science, 7, 87-94.
http://dx.doi.org/10.4081/ijas.2008.87

[18]   Di Patti, C., Di Salvo, R., Di Trapani, F. and Schimmenti, V. (2013) Les restesfauniques du déportoir. In: Lesnes, é. and Poisson, J.M., Eds., Calathamet. Archéologie et histoire d’un chateau normand en Sicile, école francaise de Rome, Rome, 283-298.

[19]   Sarà, M. (2013) Les restes fauniques du déportoir. In: Lesnes, é. and Poisson, J.M., Eds., Calathamet. Archéologie et histoire d’un chateau normand en Sicile, école francaise de Rome, Rome, 299-304.

[20]   Cooper, A. and Poinar, H.N. (2000) Ancient DNA: Do It Right or Not at All. Science, 289, 1139.
http://dx.doi.org/10.1126/science.289.5482.1139b

[21]   Yang, D.Y., Eng, B., Waye, J.S., Dudar, J.C. and Saunders, S.R. (1998) Technical Note: Improved DNA Extraction from Ancient Bones Using Silica-Based Spin Columns. American Journal of Physical Anthropology, 105, 539-543.
http://dx.doi.org/10.1002/(SICI)1096-8644(199804)105:4<539::AID-AJPA10>3.0.CO;2-1

[22]   Acutis, P.L., Colussi, S., Riina, M.V., Giovannini, T., Trisorio, S., Maniaci, M.G., Zuccon, F. and Peletto, S. (2010) Absence of the Alleles AT137RQ e ARQK176 Protective to Scrapie in Ovine Piedmontese Breeds. Proceedings of 19th SIPAOC Conference, Rimini, 16, 53.

[23]   Hofreiter, M., Jaenicke, V., Serre, D., Haeseler, A.V. and Paabo, S. (2001) DNA Sequences from Multiple Amplifications Reveal Artefacts Induced by Cytosine Deamination in Ancient DNA. Nucleic Acids Research, 29, 4793-4799.
http://dx.doi.org/10.1093/nar/29.23.4793

[24]   Acín, C., Martìn-Burriel, I., Goldmann, W., Lyahyai, J., Monzòn, M., Bolea, R., Smith, A., Rodellar, C., Badiola, J.J. and Zaragoza, P. (2004) Prion Protein Gene Polymorphisms in Healthy and Scrapie-Affected Spanish Sheep. Journal of General Virology, 85, 2103-2110.
http://dx.doi.org/10.1099/vir.0.80047-0

[25]   Hunter, N., Goldmann W., Foster J.D., Cairns, D. and Smith, G. (1997) Natural Scrapie and PrP Genotype: Case-Control Studies in British Sheep. Veterinary Record, 141, 137-140.
http://dx.doi.org/10.1136/vr.141.6.137

[26]   Sipos, W., Kraus, M., Schmool, F., Achmann, R. and Baumgartner, W. (2002) PrP Genotyping of Austrian Sheep Breeds. Journal of Veterinary Medicine Series A, 49, 415-418. http://dx.doi.org/10.1046/j.1439-0442.2002.00472.x

[27]   Reale, S., Sìneo, L., Macrì, D., Bivona, M., Arcoleo, L., Risi, M., Cosenza, M. and Vitale, F. (2014) Ancient DNA Polymorphisms Analysis to Investigate Scrapie Susceptibility in Sicilian Sheep Remains from Medieval Archaeological Sites. Proceedings of PRION 2014 Conference, 8, 67.

 
 
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