Alfred O. Ubalua^1*, Ahamefula I. Ikpeama¹, Onyinyechi D. Okeagu²

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¹ Plant Tissue Culture Unit, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI) Umudike, Umuahia, Nigeria.
² Yam Programme, National Root Crops Research Institute (NRCRI) Umudike, Umuahia, Nigeria.
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Abstract: Taro (Colocasia esculenta) and tannia (Xanthosoma sagittifolium) are commonly referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Traditionally, cocoyams are vegetatively propagated from tuber fragments, a practice that encourages pathogen distribution. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. In this study different concentrations of ripped sweet orange (Citrus sinensis) juice were screened for regeneration and multiplication of 2 months old in vitro cocoyam shoot explants. Among the concentrations, maximum numbers of roots (37 ± 5) were observed in Murashige and Skoog (MS) medium supplemented with 10% orange juice after 8 weeks in culture compared to 16 ± 4 (roots) observed in the control medium. On shoot multiplicity, 16 ± 3 shoots were induced in the control medium in contrast to 12 ± 0.8 shoots in the MS medium supplemented with 10% orange juice after 8 weeks of culture. Higher concentrations (25% and 35%) of the orange juice supplemented medium resulted in concomitant inhibition of all the growth parameters. The study successfully established that ripped orange juice could substitute the use of conventional growth hormones cytokinins (BAP) and auxins (NAA) in in vitro regeneration and rapid multiplication of cocoyam shoot explants.

Keywords: Taro, Orange Juice, Shoot Explants, In Vitro Regeneration, Rapid Multiplication

Cite this paper: O. Ubalua, A. , I. Ikpeama, A. and D. Okeagu, O. (2015) Effect of Different Concentrations of Orange Juice for in Vitro Regeneration and Multiplication of Cocoyam (Taro). American Journal of Plant Sciences, 6, 2569-2575. doi: 10.4236/ajps.2015.616259.

References

[1]   Altman, A. (2003) From Plant Tissue Culture to Biotechnology: Scientific Revolutions, Abiotic Stress Tolerance and Forestry. In Vitro Cellular Developmental Biology—Plant, 39, 75-84.
http://dx.doi.org/10.1079/IVP2002379

[2]   Ubalua, A.O. (2009) Transgenic Plants: Successes and Controversies. Biotechnology and Molecular Biology Reviews, 4, 118-127.

[3]   Jackson, G.V.H., Ball, E.A. and Arditi, J. (1977) Tissue Culture of Taro (Colocasia esculenta (L.) Schott). Journal of Horticultural Science, 52, 373-382.

[4]   Tuia, V.S. (1997) In Vitro Multiplication of Taro (Colocasia esculenta var. esculenta L. Schott), M. Agri.: University of the South Pacific.

[5]   Deo, P.C., Tyagi, A.P., Taylor, M., Becker, D.K. and Harding, R.M. (2009) Improving taro (Colocasia esculenta var. esculenta) Production Using Biotechnological Approaches. South Pacific Journal of Natural Science, 27, 6-13.
http://dx.doi.org/10.1071/SP09002

[6]   Yam, T.W., Ichihashi, S. and Arditi, J. (1991) Callus Growth and Plantlet Regeneration in Taro, Colocasia esculenta var. esculenta (L.) Schott (Araceae). Annals of Botany, 67, 317-323.

[7]   Thinh, N.T. (1997) Cryopreservation of Germplasm of Vegetatively Propagated Tropical Monocots by Vitrification. Doctoral Dissertation, Kobe University.

[8]   Verma, V.M., Cho, J.J., Aikne, J. and David, J. (2004) High Frequency Plant Production of Taro (Colocasia esculenta (L.) Schott) by Tissue Culture. Proceedings for the 4th International Crop Science Congress, Brisbane.

[9]   Deo, P.C. (2008) Somatic Embryogenesis and Transformation in Taro (Colocasia esculenta var. esculenta). Ph.D. Thesis, The University of the South Pacific, Suva.

[10]   Aguado-Santacruz, G.A., Rascón-Cruz, Q., Cabrera-Ponce, J.L., Martínez-Hernández, A., Olalde-Portugal, V. and Herrera-Estrella, L. (2002) Transgenic Plants of Blue Grama Grass, Bouteloua gracilis (H.B.K.) Lag.ex. Steud., from Microprojectile Bombardment of Highly Chlorophyllous Embryogenic Cells. Theoretical and Applied Genetics, 104, 763-771.
http://dx.doi.org/10.1007/s00122-001-0803-2

[11]   Sahrawat, A.K., Becker, D., Lütticke, S. and Lörz, H. (2003) Genetic Improvement of Wheat via Alien Gene Transfer, an Assessment. Plant Science, 165, 1147-1168.
http://dx.doi.org/10.1016/S0168-9452(03)00323-6

[12]   ul-Haq, I. (2005) Callus Proliferation and Somatic Embryogenesis in Cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 4, 206-209.

[13]   He, X., Miyasaka, S., Fitch, M.M., Zhu, Y.J. and Moore, P.H. (2004) Transformation of Taro (Colocasia esculenta) with a Rice Chitinase Gene (Abstract). In Vitro Cellular and Developmental Biology-Plant, 40, 2104.

[14]   He, X., Miyasaka, S., Fitch, M.M., Zhu, Y.J., Moore, P.H. and Zhu, Y.J. (2008) Agrobacterium tumefaciens-Mediated Transformation of Taro (Colocasia esculenta (L.) Schott) with a Rice Chitinase Gene for Improved Tolerance to a Fungal Pathogen Sclerotium rolfsii. Plant Cell Reports, 27, 903-909.
http://dx.doi.org/10.1007/s00299-008-0519-8

[15]   Straus, M.S., Michaud, J.D. and Arditi, J. (1979) Seed Storage and Germination and Seedling Proliferation in Taro (Colocasia esculenta (L.) Schott). Annals of Botany, 43, 603-612.

[16]   Wang, J.K. (1983) Taro: A Review of Colocasia esculenta and Its Potential. University of Hawaii, Honolulu.

[17]   Harms, C.L. and Oplinger, E.S. (1988) Plant Growth Regulators: Their Use in Crop Production. North Central Region Extension Publication 303, Wisconsin.

[18]   Skoog, F. and Miller, R.A. (1957) Chemical Regulations of Growth and Organ Formation in Plant Tissue Culture in Vitro. Symposia of the Society for Experimental Biology, 11, 118-130.

[19]   Nair, R.R., Kavitha, M., Thilaga, S. and Ganesh, D. (2012) Conservation and in Vitro Multiplication of Highly Endangered Indian Traditional Medicinal Plant (Morinda reticulata Gamble) through Nodal Explants. Plant Knowledge Journal, 1, 46-51.

[20]   USDA (2008) USDA National Nutrient Database for Standard Reference, Release 21. NDB 09209.

[21]   Ali, F.M., Ahsan, M., Saeed, N.A., Ahmed, M., Ali, Q., Kanwal, N., Tehseen, M.M., Ijaz, U., Bibi, I. and Niazi, N.K. (2014) Establishment and Optimization of Callus-to-Plant Regeneration System Using Mature Andimmature Embryos of Maize (Zea mays). International Journal of Agricultural Biology, 16, 11-117.

[22]   Ubalua, A.O. and Chukwu, L.I. (2008) Potentials and Constraints of Cocoyam Production in Nigeria. Proceedings of the 42nd Annual Conference, Agricultural Society of Nigeria (ASN), Ebonyi State University, Abakaliki, 19-23 October 2008.

[23]   Wilson, J.E. (1979) Cocoyam Breeding by Flower Induction, Pollination, and Germination. Manual Ser No. 4, IITA, Ibadan.

[24]   Alamu, S., McDavid, C.R. and Duncan, E.J. (1982) Production of Viable Gibberellic Acid-Treated Tannia (Xanthosoma sagittifolium) Plants. Tropical Agriculture, 59, 323-334.

[25]   Agueguia, A. and Nzietchueng, S. (1984) Production of Hybrid Xanthosoma sagitifolium and Test for Resistance to Phythium myriotylium. In: Terry, E.R., Doku, E.V., Arene, O.B. and Mahungu, N.M., Eds., Tropical Root Crops: Production and Uses in Africa, Proceedings of the 2nd Triennial Symposium of the International Society for Tropical Root Crops, Africa Branch, Douala, IDRC, Ottawa, Vol. 221, 169-171.

[26]   Tambong, J.T. and Meboka, M. (1994) Cocoyam (Xanthosoma sagittifolium (L.)) Hybridization Studies: Pollen Viability and Seed Germination. Acta Horticulturae, 380, 448-459.
http://dx.doi.org/10.17660/ActaHortic.1994.380.69

[27]   Alkhateeb, A. (2008) Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.). American Journal of Biotechnology and Biochemistry, 4, 19-23.
http://dx.doi.org/10.3844/ajbbsp.2008.19.23

[28]   Saad, A.I.M. and Elshahed, A.M. (2012) Plant Tissue Culture Media. In: Leva, A. and Rinaldi, L.M.R., Eds., Recent Advances in Plant in Vitro Culture, Chap. 2, InTech, Winchester, 29-40.

[29]   Ikram-ul-Hag, A.Z., Taseer, M.G., Mukesh, D.M.U. and Ali, S.M. (2011) Effects of Different Fruit Juices Used as Carbon Source on Cucumber Seedling under in Vitro Cultures. African Journal biotechnology, 10, 7404-7408.

[30]   Manawadu, I., Dahanayake, N., Senanayake, S.G.N. (2014) Effects of Different Natural Organic Additives on in Vitro Shoot Regeneration of Raphanus sativus L. var. Beeralu. Journal of Agricultural Science and Technology, A4, 219-223.

[31]   Rahman, A., Islam, M.O., Prodhan, A. and Ichihashi, S. (2004) Effects of Complex Organic Extracts on Plantlet Regeneration from PLBs and Plantlet Growth in the Doritaenopsis Orchid. Japan Agricultural Research Quarterly, 38, 55-59.
http://dx.doi.org/10.6090/jarq.38.55