OJVM  Vol.5 No.10 , October 2015
VEGF System in Dog’s Thymus—Temporal Expression
Abstract: Given the importance of the thymus to the immune system and the limited knowledge about its development and involution processes, this study evaluated the expression of the vascular endo-thelial growth factor, a key angiogenic factor for the formation of vascular tissue bed and modulation of functions related to cell proliferation and migration, hormone synthesis, and immunomodulation. In this study, thymus samples of dog fetuses (30, 40, 50 and 60 days old), and young (180 days old) and adult (365 days old) dogs were submitted to real-time PCR and Western blot. Time-dependent changes were observed for proteins and genes associated with the development of the vascular system, such as VEGF-A, VEGF-C, Flt-1, KDR, FLT-4, and EG-VEGF. This result demonstrates that these system components play different roles during development and involution of the thymus. Possible functions, such as the modulatory effect on vasculogenesis and microenvironment, influence the proliferation and differentiation of thymocytes, T cells maturation, cell-cell interaction and hormone secretion.
Cite this paper: Agreste, F. , Bombonato, P. , Nogueira, K. , Santos, A. , Barreto-Vianna, A. and Lima, E. (2015) VEGF System in Dog’s Thymus—Temporal Expression. Open Journal of Veterinary Medicine, 5, 211-217. doi: 10.4236/ojvm.2015.510028.

[1]   Sinkora, J., Rehakova, Z., Sinkora, M., Cukrowska, B. and Tlaskalova-Hogenova, H. (2002) Early Development of Immune System in Pigs. Veterinary Immunology, 87, 301-306.

[2]   Kiseleva, E.P., Krylov, A.V., Lyudyno, V.I. and Suvorov, A.N. (2005) Effect of VEGF on Mouse Thymocyte Proliferation and Apoptosis in Vitro. Bulletin of Experimental Biology and Medicine, 139, 576-579.

[3]   Bustin, S.A. (2000) Absolute Quantification of mRNA Using Real-Time Reverse Transcription Polymerase Chain Reaction Assays. Journal of Molecular Endocrinology, 25, 169-193.

[4]   Rasmussen, R. (2001) Quantification on the Light Cycler. In: Meuer, S., Wittwer, C. and Nakagawara, K., Eds., Rapid Cycle Real-Time PCR, Methods and Applications, Springer Press, Heidelberg, 21-34.

[5]   Cuddihy, A.R., Ge, S., Zhu, J., et al. (2009) VEGF-Mediated Cross-Talk within the Neonatal Murine Thymus. Blood, 113, 2723-2731.

[6]   Agreste, F.R., Moriconi, P.R., Barreto-Vianna, A.R.C., Santos-Leonardo, A., Bombonato, P.P. and Lima, E.M.M. (2013) Is the Vascular Growth Curve of Thymus of Dogs (Canis familiaris) Time-Dependent? Australian Journal of Basic and Applied Science, 7, 334-342.

[7]   Cimpean, A.M., Raica, M., Encica, S., Cornea, R. and Bocan, V. (2008) Immunohistochemical Expression of Vascular Endothelial Growth Factor A (VEGF), and Its Receptors (VEGFR1, 2) in Normal and Pathologic Conditions of the Human Thymus. Anatomischer Anzeiger, 190, 238-245.

[8]   Watanabe, N., Wang, Y.H., Lee, H.K., et al. (2005) Hassall’s Corpuscles Instruct Dendritic Cells to Induce CD4+CD25+ Regulatory T Cells in Human Thymus. Nature, 436, 1181-1185.

[9]   Olsson, A.K., Dimberg, A., Kreuger, J. and Claesson-Welsh, L. (2006) VEGF Receptor Signalling? In Control of Vascular Function. Nature Reviews: Molecular Cell Biology, 7, 359-371.

[10]   Ji, R.C. (2009) Lymph Node Lymphangiogenesis, a New Concept for Modulating Tumor Metastasis and Inflammatory Process. Histology and Histopathology, 24, 377-384.

[11]   Sabattini, S. and Bettini, G. (2009) An Immunohistochemical Analysis of Canine Haemangioma and Haemangiosarcoma. Journal of Comparative Pathology, 140, 158-168.

[12]   Shibuya, M. (2001) Structure and Dual Function of Vascular Endothelial Growth Factor Receptor-1 (Flt-1). The International Journal Biochemistry & Cell Biology, 33, 409-420.

[13]   Takahashi, H. and Shibuya, M. (2005) The Vascular Endothelial Growth Factor (VEGF)/VEGF Receptor System and Its Role under Physiological and Pathological Conditions. Clinical Science, 109, 227-241.

[14]   Ribatti, D., Nico, B., Crivellato, E., Roccaro, A.M. and Vacca, A. (2007) The History of the Angiogenic Switch Concept. Leukemia, 21, 44-52.

[15]   LeCouter, J., Kowalski, J., Foster, J., Hass, P., Zhang, Z., Dillard-Telm, L. and Ferrara, N. (2001) Identification of an Angiogenic Mitogen Selective for Endocrine Gland Endothelium. Nature, 412, 877-884.

[16]   Hoffmann, P., Feige, J.J. and Alfaidy, N. (2006) Expression and Oxygen Regulation of Endocrine Gland-Derived Vascular Endothelial Growth Factor/Prokineticin-1 and Its Receptors in Human Placenta during Early Pregnancy. Endocrinology, 147, 1675-1684.

[17]   Hoffmann, P., Feige, J.J. and Alfaidy, N. (2007) Placental Expression of EG-VEGF and Its Receptors PKR1 (Prokineticin Receptor-1) and PKR2 throughout Mouse Gestation. Placenta, 28, 1049-1058.