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 AJPS  Vol.6 No.16 , October 2015
Morphological and Biochemical Changes in Ginseng Seedling Roots Affected with Stripe Symptoms
Abstract: A unique symptom of longitudinal red stripes on the surface of one-year-old ginseng roots was studied to determine the morphological and biochemical changes taking place. Light and scanning electron microscopy, measurements of phenolic and mineral element content, and enzyme activity, were compared between healthy and stripe-affected root tissues. Light microscopy revealed that the root epidermis had ruptured and fissures extended for 3 - 4 cell layers into the cortex. Phenolic compounds accumulated in the epidermal cells which stained with Toluidine blue 0. Total phenolic content was higher in tissues from striped roots compared to healthy roots and HPLC profiles showed increases in a number of specific phenolic compounds. Analysis of epidermal tissues by SEM-EDX for mineral element content showed a marked increase in levels of iron, silicon and aluminum and a decline in potassium in striped root tissues. The activity of the enzymes phenylalanine ammonia lyase and peroxidase were also found to be higher in striped root tissues. Striping of ginseng roots is a physiological condition caused by a rupture of the epidermis due to rapid growth of underlying cells, which results in phenolic accumulation and sequestration of several minerals. Further oxidation causes a visible red striping on the root surface.
Cite this paper: K. Punja, Z. and Rahman, M. (2015) Morphological and Biochemical Changes in Ginseng Seedling Roots Affected with Stripe Symptoms. American Journal of Plant Sciences, 6, 2550-2560. doi: 10.4236/ajps.2015.616257.
References

[1]   Punja, Z.K. (2011) American Ginseng: Research Developments, Opportunities, and Challenges. Journal of Ginseng Research, 35, 368-374.
http://dx.doi.org/10.5142/jgr.2011.35.3.368

[2]   Proctor, J.T.A. and Bailey, W.G. (1987) Ginseng: Industry, Botany, and Culture. Horticultural Reviews, 9, 188-236.
http://dx.doi.org/10.1002/9781118060827.ch6

[3]   Punja, Z.K. (1997) Fungal Pathogens of American Ginseng (Panax quinquefolius L.) in British Columbia, Canada. Canadian Journal of Plant Pathology, 19, 301-306.
http://dx.doi.org/10.1080/07060669709500528

[4]   Schluter, C. and Punja, Z.K. (2002) Genetic Diversity among Natural and Cultivated Field Populations and Seed Lots of American Ginseng (Panax quinquefolius L.) in Canada. International Journal of Plant Science, 163, 427-439.
http://dx.doi.org/10.1086/339512

[5]   Lee, T.S., Mok, S.K., Seon, S.K., Yoon, J.H., Baek, N.I. and Choe, J. (2004) Accumulation of Crude Lipids, Phenolic Compounds, and Iron in Rusty Ginseng Root Epidermis. Journal of Ginseng Research, 28, 157-164.
http://dx.doi.org/10.5142/JGR.2004.28.3.157

[6]   Campeau, C., Proctor, J.T.A., Murr, D.P. and Schooley, J. (2003) Characterization of North American Ginseng Rust-Spots and the Effects of Ethephon. Journal of Ginseng Research, 27, 188-194.
http://dx.doi.org/10.5142/JGR.2003.27.4.188

[7]   Yingping, W., Zhihong, L., Yanjun, S., Shiwei, G., Shuzhen, T. and Zhorong, L. (1997) Studies on the Genesis of Ginseng Rust Spots. Korean Journal of Ginseng Science, 21, 69-77.

[8]   Punja, Z.K., Wan, A., Goswami, R., Verma, N., Rahman, M., Barasubiye, T., Seifert, K. and Lévesque, C.A. (2007) Diversity of Fusarium Species Associated With Discolored Ginseng Roots in British Columbia. Canadian Journal Plant Pathology, 29, 340-353.
http://dx.doi.org/10.1080/07060660709507480

[9]   Reeleder, R.D., Roy, R. and Capell, B.B. (1999) Seed and Root Rots of Ginseng (Panax quinquefolius) Caused by Cylindrocarpon destructans and Fusarium spp. Journal of Ginseng Research, 26, 151-158.

[10]   Punja, Z.K., Wan, A., Lieppi, L., Goswami, R.S. and Jayaraj, J. (2013) Growth, Pathogenicity, Infection Behaviour, and Genetic Diversity of Rhexocercosporidium Isolates Originating From Ginseng Fields in British Columbia. Canadian Journal of Plant Pathology, 35, 503-513.
http://dx.doi.org/10.1080/07060661.2013.843315

[11]   Gerrity, R.G. and Forbes, G.W. (2003) Microwave Processing in Diagnostic Electron Microscopy. Microscopy Today, 11, 38-41.

[12]   Dahlquist, R.L. and Knoll, J.W. (1978) Inductively Coupled Plasma-Atomic Emission Spectrometry: Analysis of Biological Materials and Soils for Major, Trace, and Ultra-Trace Elements. Applied Spectroscopy, 32, 1-29.
http://dx.doi.org/10.1366/000370278774331828

[13]   Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent. Methods in Enzymology, 299, 152-178.
http://dx.doi.org/10.1016/S0076-6879(99)99017-1

[14]   Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254.
http://dx.doi.org/10.1016/0003-2697(76)90527-3

[15]   Hartz, T.K., Johnstone, P.R. and Nunez, J.J. (2005) Production Environment and Nitrogen Fertility Affect Carrot Cracking. HortScience, 40, 611-615.

[16]   Hiller, L.K., Koller, D.C. and Thornton, R.E. (1985) Physiological Disorders of Potatoes. In: Li, P.H., Ed., Potato Physiology, Academic Press, New York, 389-455.

[17]   Jefferies, R.A. and MacKerron, D.K.L. (1987) Observations on the Incidence of Tuber Growth Cracking in Relation to Weather Patterns. Potato Research, 30, 613-623.
http://dx.doi.org/10.1007/BF02367642

[18]   Emmons, C.L. and Scott, J.W. (1996) Factors Affecting Cuticle Cracking in Tomato. Proceedings of the Florida State Horticultural Sciences, 109, 178-182.

[19]   Peschel, S. and Knoche, M. (2005) Characterization of Microcracks in the Cuticle of Developing Sweet Cherry Fruit. Journal of the American Society for Horticultural Science, 130, 487-495.

[20]   Ramirez, E.C., Whitaker, J.R. and Virador, V.M. (2002) Polyphenol Oxidase. In: Whitaker, J.R., Voragen, A.G.J. and Wong, D.W.S., Eds., Handbook of Food Enzymology, Marcel Dekker, New York, 509-523.
http://dx.doi.org/10.1201/9780203910450.ch39

[21]   Crisosto, G.W., Johnson, R.S. and Luza, J. (1993) Incidence of Physical Damage on Peach and Nectarine Skin Discoloration Development: Anatomical Studies. Journal of the American Society for Horticultural Science, 118, 796-800.

[22]   Boudet, A.-M. (2007) Evolution and Current Status of Research in Phenolic Compounds. Phytochemistry, 68, 2722- 2735.
http://dx.doi.org/10.1016/j.phytochem.2007.06.012

[23]   Nicholson, R.L. and Hammerschmidt, R. (1998) Phenolic Compounds and Their Role in Disease Resistance. Annual Review of Phytopathology, 30, 369-389.
http://dx.doi.org/10.1146/annurev.py.30.090192.002101

[24]   Rice-Evans, C.A., Miller, N.J. and Paganga, G. (1996) Structure-Antioxidant Activity Relationship of Flavonoids and Phenolic Acids. Free Radical Biology in Medicine, 20, 933-956.
http://dx.doi.org/10.1016/0891-5849(95)02227-9

[25]   Brune, M., Rossander, L. and Hallberg, L. (1989) Iron Absorption and Phenolic Compounds: Importance of Different Phenolic Structures. European Journal of Clinical Nutrition, 43, 547-558.

[26]   Cheng, G.W. and Crisosto, C.H. (1997) Iron-Polyphenol Complex Formation and Skin Discoloration in Peaches and Nectarines. Journal of the American Society for Horticultural Science, 122, 95-99.

[27]   Lavid, N., Schwartz, A., Yarden, O. and Tel-Or, E. (2000) The Involvement of Polyphenols and Peroxidase Activities in Heavy-Metal Accumulation by Epidermal Glands of the Waterlily (Nymphaeceae). Planta, 212, 323-331.
http://dx.doi.org/10.1007/s004250000400

[28]   Khokhar, S. and Richard, K.A.O. (2003) Iron Binding Characteristics of Phenolic Compounds: Some Tentative Structure-Activity Relations. Food Chemistry, 81, 133-140.
http://dx.doi.org/10.1016/S0308-8146(02)00394-1

[29]   Poovaiah, B.W. (1973) Peroxidase Activity in the Abscission Zone of Bean Leaves during Abscission. Plant Physiology, 52, 263-267.
http://dx.doi.org/10.1104/pp.52.3.263

[30]   Goswami, R. and Punja, Z.K. (2008) Molecular and Biochemical Characterization of Defense Responses in Ginseng (Panax quinquefolius) Roots Challenged with Fusarium equiseti. Physiological and Molecular Plant Pathology, 72, 10-20.
http://dx.doi.org/10.1016/j.pmpp.2008.04.006

 
 
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