Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast
Author(s)
Maxim Sergeevich Makarov*,
Natalya Valerjevna Borovkova,
Olga Ivanovna Konushko,
Valery Borisovich Khvatov
Affiliation(s)
Scientific Emergency Aim Institute of N. V. Sklifosovsky, Health Department of Moscow, Moscow, Russia.
Scientific Emergency Aim Institute of N. V. Sklifosovsky, Health Department of Moscow, Moscow, Russia.
ABSTRACT
We studied in vitro attachment, proliferation and survival of сadaver skin fibroblasts in collagen bands, dermal matrix and cancellous demineralized bone, enriched with platelet components. It was shown, that PRP enhanced revitalization of collagen grafts, especially followed by components of activated or freezed platelets. Fibroblasts had the best rate of proliferative activity in response to 150 pg platelet derived growth factor (PDGF), released from 100 million platelets with granules and measured for 100 × 103 cultivated cells. The use of platelet-derived material could increase fibroblast proliferation activity for 1.5-3 times in all types of collagen transplants without damage or decay of cell’s biological value.
We studied in vitro attachment, proliferation and survival of сadaver skin fibroblasts in collagen bands, dermal matrix and cancellous demineralized bone, enriched with platelet components. It was shown, that PRP enhanced revitalization of collagen grafts, especially followed by components of activated or freezed platelets. Fibroblasts had the best rate of proliferative activity in response to 150 pg platelet derived growth factor (PDGF), released from 100 million platelets with granules and measured for 100 × 103 cultivated cells. The use of platelet-derived material could increase fibroblast proliferation activity for 1.5-3 times in all types of collagen transplants without damage or decay of cell’s biological value.
Cite this paper
Makarov, M. , Borovkova, N. , Konushko, O. and Khvatov, V. (2015) Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast. Journal of Biosciences and Medicines, 3, 80-87. doi: 10.4236/jbm.2015.310011.
Makarov, M. , Borovkova, N. , Konushko, O. and Khvatov, V. (2015) Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast. Journal of Biosciences and Medicines, 3, 80-87. doi: 10.4236/jbm.2015.310011.
References
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http://dx.doi.org/10.1115/1.2165699 [2] Ahlfors, J.E. and Billiar, K.L. (2007) Biomechanical and Biochemical Characteristics of a Human Fibroblast Produced and Remodeled Matrix. Biomaterials, 28, 2183-2191.
http://dx.doi.org/10.1016/j.biomaterials.2006.12.030 [3] Friedl, P. and Brocker, E.B. (2000) The Biology of Cell Locomotion within Three-Dimensional Extracellular Matrix. Cellular and Molecular Life Sciences CMLS, 57, 41-64. http://dx.doi.org/10.1007/s000180050498 [4] Jiang, H., Rhee, S., Ho, C.-H. and Grinnell, F. (2008) Distinguishing Fibroblast Promigratory and Procontractile Growth Factor Environments in 3D Collagen Matrices. The FASEB Journal, 22, 2151-2160.
http://dx.doi.org/10.1096/fj.07-097014 [5] Pedersen, J.A., Boschetti, F. and Swartz, M.A. (2007) Effects of Extracellular Fiber Architecture on Cell Membrane Shear Stress in a 3D Fibrous Matrix. Journal of Biomechanics, 40, 1484-1492.
http://dx.doi.org/10.1016/j.jbiomech.2006.06.023 [6] Grinnell, F. (2003) Fibroblast Biology in Three-Dimensional Collagen Matrices. Trends in Cell Biology, 13, 264-269.
http://dx.doi.org/10.1016/S0962-8924(03)00057-6 [7] Anitua, E., Andia, I., Ardanza, B., Nurden, P. and Nurden, A.T. (2004) Autologousplatelets as a Source of Proteins for Healing and Tissue Regeneration. Thromb Haemost, 91, 4-15. [8] Mazurov, A.V. (2011) Physiology and Pathology of Platelets. Litterra, Moscow. [9] Toit, D.F., Kleintjes, W.G. and Otto, M.J. (2007) Soft and Hard-Tissue Augmentation with Platelet-Rich Plasma: Tissue Culture Dynamics, Regeneration and Molecular Biology Perspective. International Journal of Shoulder Surgery, 1, 64-73.
http://dx.doi.org/10.4103/0973-6042.32923 [10] Dees, C., Akhmetshina, A., Zerr, P. Reich, N., Palumbo, K., Horn, A., Jüngel, A., Beyer, C., Kronke, G. and Zwerina, J. (2011) Platelet-Derived Serotonin Links Vascular Disease and Tissue Fibrosis. Journal of Experimental Medicine, 208, 961-972.
http://dx.doi.org/10.1084/jem.20101629 [11] Wang, Z., Ahmad, A., Li, Y., Kong, D., Azmi, A.S., Banerjee, S. and Sarkar, F.H. (2010) Emerging Roles of PDGF-D Signaling Pathway in Tumor Development and Progression. Biochimica et Biophysica Acta, 1806, 122-130.
http://dx.doi.org/10.1016/j.bbcan.2010.04.003 [12] Harrison, P. and Mumford, A. (2009) Screening Tests of Platelet Function: Update on Their Appropriate Uses for Diagnostic Testing. Seminars in Thrombosis and Hemostasis, 35, 150-157.
http://dx.doi.org/10.1055/s-0029-1220323 [13] Khubutia, M.Sh., Andreev, Yu.V., Borovkova, N.V., Khvatov, V.B., Mironov, A.S., Zirkova, E.A., Ponomarev, I.N. and Makarov M.S. (2014) Method of Dermal Matrix Production. Patent RU 2,524,619. A61K35/36, A61L27/36. [14] Bykov, V.A., Denisov-Nikolsky, Yu.I., Denisova, L.A., Matvejchuk, I.V. and Rozanov, V.V. (2006) Method of Bone Implant Production. Patent RU 2,268,060. A61K35/32, A01N1/00. [15] Makarov, M.S., Khvatov, V.B., Konushko, O.I., Storogeva, M.V., Borovkova, N.V. and Ponomarev, I.N. (2013) Method of Morphofunctional Analysis of Biotransplant Cell Components. Patent RU 2,484,472. G01N33/48. [16] Makarov, M.S., Kobzeva, E.N., Vysochin, I.V., Borovkova, N.V. and Khvatov, V.B. (2014) Morphofunctional Analysis of Human Platelets by Vital Staining. Bulletin of Experimental Biology and Medicine, 156, 409-412.
http://dx.doi.org/10.1007/s10517-014-2360-0 [17] Richards-Kortum, R. and Sevick-Muraca, E.M. (1996) Quantitative Optical Spectroscopy for Tissue Diagnosis. Annual Review of Physical Chemistry, 47, 555-606.
http://dx.doi.org/10.1146/annurev.physchem.47.1.555 [18] Makarov, M.S., Storogeva, M.V., Konushko, O.I., Borovkova, N.V. and Khvatov, V.B. (2013) Effect of Concentration of Platelet-Derived Growth Factor on Proliferative Activity of Human Fibroblasts. Bulletin of Experimental Biology and Medicine, 155, 576-580. http://dx.doi.org/10.1007/s10517-013-2199-9 [19] Schubert, P. and Devine, D.V. (2010) Towards Targeting Platelet Storage Lesion-Related Signaling Pathways. Blood Transfus, 8, 69-72. [20] Monteiro, G.A., Rodriguez, N.L., Delossantos, A.I. and Wagner, C.T. (2013) Short-Term in Vivo Biological and Mechanical Remodeling of Porcine Acellular Dermal Matrices. Journal of Tissue Engineering, 4, 2041731413490182.
http://dx.doi.org/10.1177/2041731413490182
[1] Chandran, P.L. and Barocas, V.H. (2006) Affine versus Non-Affine Fibril Kinematics in Collagen Networks: Theoretical Studies of Network Behavior. Journal of Biomechanical Engineering, 128, 259-270.
http://dx.doi.org/10.1115/1.2165699 [2] Ahlfors, J.E. and Billiar, K.L. (2007) Biomechanical and Biochemical Characteristics of a Human Fibroblast Produced and Remodeled Matrix. Biomaterials, 28, 2183-2191.
http://dx.doi.org/10.1016/j.biomaterials.2006.12.030 [3] Friedl, P. and Brocker, E.B. (2000) The Biology of Cell Locomotion within Three-Dimensional Extracellular Matrix. Cellular and Molecular Life Sciences CMLS, 57, 41-64. http://dx.doi.org/10.1007/s000180050498 [4] Jiang, H., Rhee, S., Ho, C.-H. and Grinnell, F. (2008) Distinguishing Fibroblast Promigratory and Procontractile Growth Factor Environments in 3D Collagen Matrices. The FASEB Journal, 22, 2151-2160.
http://dx.doi.org/10.1096/fj.07-097014 [5] Pedersen, J.A., Boschetti, F. and Swartz, M.A. (2007) Effects of Extracellular Fiber Architecture on Cell Membrane Shear Stress in a 3D Fibrous Matrix. Journal of Biomechanics, 40, 1484-1492.
http://dx.doi.org/10.1016/j.jbiomech.2006.06.023 [6] Grinnell, F. (2003) Fibroblast Biology in Three-Dimensional Collagen Matrices. Trends in Cell Biology, 13, 264-269.
http://dx.doi.org/10.1016/S0962-8924(03)00057-6 [7] Anitua, E., Andia, I., Ardanza, B., Nurden, P. and Nurden, A.T. (2004) Autologousplatelets as a Source of Proteins for Healing and Tissue Regeneration. Thromb Haemost, 91, 4-15. [8] Mazurov, A.V. (2011) Physiology and Pathology of Platelets. Litterra, Moscow. [9] Toit, D.F., Kleintjes, W.G. and Otto, M.J. (2007) Soft and Hard-Tissue Augmentation with Platelet-Rich Plasma: Tissue Culture Dynamics, Regeneration and Molecular Biology Perspective. International Journal of Shoulder Surgery, 1, 64-73.
http://dx.doi.org/10.4103/0973-6042.32923 [10] Dees, C., Akhmetshina, A., Zerr, P. Reich, N., Palumbo, K., Horn, A., Jüngel, A., Beyer, C., Kronke, G. and Zwerina, J. (2011) Platelet-Derived Serotonin Links Vascular Disease and Tissue Fibrosis. Journal of Experimental Medicine, 208, 961-972.
http://dx.doi.org/10.1084/jem.20101629 [11] Wang, Z., Ahmad, A., Li, Y., Kong, D., Azmi, A.S., Banerjee, S. and Sarkar, F.H. (2010) Emerging Roles of PDGF-D Signaling Pathway in Tumor Development and Progression. Biochimica et Biophysica Acta, 1806, 122-130.
http://dx.doi.org/10.1016/j.bbcan.2010.04.003 [12] Harrison, P. and Mumford, A. (2009) Screening Tests of Platelet Function: Update on Their Appropriate Uses for Diagnostic Testing. Seminars in Thrombosis and Hemostasis, 35, 150-157.
http://dx.doi.org/10.1055/s-0029-1220323 [13] Khubutia, M.Sh., Andreev, Yu.V., Borovkova, N.V., Khvatov, V.B., Mironov, A.S., Zirkova, E.A., Ponomarev, I.N. and Makarov M.S. (2014) Method of Dermal Matrix Production. Patent RU 2,524,619. A61K35/36, A61L27/36. [14] Bykov, V.A., Denisov-Nikolsky, Yu.I., Denisova, L.A., Matvejchuk, I.V. and Rozanov, V.V. (2006) Method of Bone Implant Production. Patent RU 2,268,060. A61K35/32, A01N1/00. [15] Makarov, M.S., Khvatov, V.B., Konushko, O.I., Storogeva, M.V., Borovkova, N.V. and Ponomarev, I.N. (2013) Method of Morphofunctional Analysis of Biotransplant Cell Components. Patent RU 2,484,472. G01N33/48. [16] Makarov, M.S., Kobzeva, E.N., Vysochin, I.V., Borovkova, N.V. and Khvatov, V.B. (2014) Morphofunctional Analysis of Human Platelets by Vital Staining. Bulletin of Experimental Biology and Medicine, 156, 409-412.
http://dx.doi.org/10.1007/s10517-014-2360-0 [17] Richards-Kortum, R. and Sevick-Muraca, E.M. (1996) Quantitative Optical Spectroscopy for Tissue Diagnosis. Annual Review of Physical Chemistry, 47, 555-606.
http://dx.doi.org/10.1146/annurev.physchem.47.1.555 [18] Makarov, M.S., Storogeva, M.V., Konushko, O.I., Borovkova, N.V. and Khvatov, V.B. (2013) Effect of Concentration of Platelet-Derived Growth Factor on Proliferative Activity of Human Fibroblasts. Bulletin of Experimental Biology and Medicine, 155, 576-580. http://dx.doi.org/10.1007/s10517-013-2199-9 [19] Schubert, P. and Devine, D.V. (2010) Towards Targeting Platelet Storage Lesion-Related Signaling Pathways. Blood Transfus, 8, 69-72. [20] Monteiro, G.A., Rodriguez, N.L., Delossantos, A.I. and Wagner, C.T. (2013) Short-Term in Vivo Biological and Mechanical Remodeling of Porcine Acellular Dermal Matrices. Journal of Tissue Engineering, 4, 2041731413490182.
http://dx.doi.org/10.1177/2041731413490182