AiM  Vol.5 No.10 , September 2015
PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba
Abstract: Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba.
Cite this paper: Armas, Y. , Fernández, C. , Díaz, R. , Stefano, N. , Wissmann, G. and Calderón, E. (2015) PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba. Advances in Microbiology, 5, 724-729. doi: 10.4236/aim.2015.510076.

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