AS  Vol.6 No.9 , September 2015
Estimation of Mycotoxin Multiple Contamination in Mexican Hybrid Seed Maize by HPLC-MS/MS
ABSTRACT
In Mexico, the presence of mycotoxins in chemical treated seed maize by sowing is not well known, despite the need to improve the quality and corn safe for human consumption. It collected twenty-five genotypes maize samples from Morelos State in the spring of 2013, all of them treated with synthetic colors (pink, green, yellow), fungicides and insecticides. Two samples (synthetic seed and hybrid commercial) were selected for analysis of twenty-two mycotoxins by LC-MS/MS and AFB1 determination by liquid chromatography and fluorescence detection (HPLC-FLD). The results of the 25 samples showed the presence of Aflatoxin B1 in 25% of samples in a ranged concentration between 2 to 6 μg&middotkg-1, and average of 4.1-1 μg&middotkg, which were within the allowed limits by national and international legislation. Twenty-two mycotoxins were found in levels ranging between 791.7 and 891.2 μg&middotkg. The content average in both samples was for total aflatoxins (AFB1, AFB2, AFG1, AG2) of 16.95 μg&middotkg, with G aflatoxins the most prevalence. Twelve trichothecenes (Nivalenol, Neosolanol, Fusarenone X, DAS, HT-2, FB1, FB2, FB3, T-2, Zearalenone, ZEA2, ZEA3) were in a level of 292.7 μg&middotkg-1, Enniantine 8.6 μg&middotkg-1, Sterigmatocystin 6.5 μg&middotkg-1, Roquefortine C, 2.9 μg&middotkg-1. Ochratoxin 8.8 μg&middotkg-1 and Mycophenolic acid at 535 μg&middotkg-1 were the highest content. The synthetic color present in seeds analyzed inhibited a good purification in the extracted mycotoxin by optimizing the step in HPLC-MS/MS quantification system. The information generated in this study would be useful in breeding programs in order to improve the sanitary quality and also to investigate the final contamination of agricultural products with multiple mycotoxin contamination.

Cite this paper
Peña Betancourt, S. , Carranza, B. and Manzano, E. (2015) Estimation of Mycotoxin Multiple Contamination in Mexican Hybrid Seed Maize by HPLC-MS/MS. Agricultural Sciences, 6, 1089-1097. doi: 10.4236/as.2015.69104.
References
[1]   (2014) Centro de Estudios para el consumo rural sostenible y la soberanía alimentaria. www.cedrssa.gob.mx

[2]   Duarte-Vogel, S. and Villamil-Jiménez, L.C. (2006) Micotoxins in Public Health. Revista de Salud Pública, 8, 129-135.
http://dx.doi.org/10.1590/S0124-00642006000400011

[3]   Turkington, T.K., Clear, R.M., Demeke, T., Lange, R., Xi, K. and Kumar, K. (2011) Isolation of Fusarium graminearum from Cereal, Grass and Corn Residues from Alberta, 2001-2003. Canadian Journal of Plant Pathology, 33, 179-186.

[4]   Galvano, F. and Ritieni, A. (2005) Mycotoxins in the Human Food Chain. In: Diez, D., Ed., Mycotoxin Blue Book, Nothinghan University Press, Nothingham, 187.

[5]   Naresh, M., Hope, R., Carrns, V. and Aldred, D. (2003) Post-Harvest Fungal Ecology: Impact of Fungal Growth and Mycotoxin Accumulation in Stored Grain. European Journal of Plant Pathology, 109, 723-730.
http://dx.doi.org/10.1023/A:1026082425177

[6]   International Agency for Research on Cancer (IARC) (1993) IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. Volume 56, IARC Working Group, WHO, Lyon.

[7]   Antonissen, G., Martel, A., Pasnans, F., Ducatelle, R., Verbrugghe, E., Vandenbroucke, V., et al. (2014) The Impact of Fusarium Mycotoxin on Human and Animal Host Susceptibility to Infectious Diseases. Toxins, 6, 340-352.

[8]   Rajmon, R., Sedmikova, M., Jílek, F., Koubková, M., Härtlová, H., Bárta, I. and Smerák, P. (2001) Combined Effects of Repeated Low Doses of Aflatoxin B1 and T-2 Toxin on the Chinese Hamster. Veterinary Medicine-Czech, 46, 301-307.

[9]   Streit, E., Schwab, C., Sulyok, M., Naehrer, K., Krska, R. and Schatzmayr, G. (2013) Multi-Mycotoxin Screening Reveals the Occurrence of 139 Different Secondary Metabolites in Fed and Feed Ingredients. Toxins, 5, 504-523.
http://dx.doi.org/10.3390/toxins5030504

[10]   Pereyra, S.A. and Dill-Macky, R. (2008) Colonization of the Residues of Diverse Plant Species by Gibberella zeae and Their Contribution to Fusarium Head Blight Inoculum. Plant Disease, 92, 800-807.

[11]   Oldenburg, E., Kramer, S., Schrader, S. and Weinert, J. (2008) Impact of the Earthworm Lumbricus terrestris on the Degradation of Fusarium-Infected and Deoxynivalenol-Contaminated Wheat Straw. Soil Biology and Biochemistry, 40, 3049-3053.
http://dx.doi.org/10.1016/j.soilbio.2008.09.004

[12]   Vogelgsang, S., Hecker, A., Musa, T., Dorn, B. and Forrer, H.-R. (2011) On-Farm Experiments over 5 Years in a Grain Maize/Winter Wheat Rotation: Effect of Maize Residue Treatments on Fusarium graminearum Infection and Deoxynivalenol Contamination in Wheat. Mycotoxin Research, 27, 81- 96.
http://dx.doi.org/10.1007/s12550-010-0079-y

[13]   Lee, H.B., Patriarca, A. and Magan, N. (2015) Alternaria in Food: Ecophysiology, Mycotoxin Production and Toxicology. Mycobiology, 43, 93-106.
http://dx.doi.org/10.5941/MYCO.2015.43.2.93

[14]   Carrillo, M.G.V., Pérez Camarillo, J.P., Hernández Casillas, J.M., de la Luz Marrufo Diaz, M. and Ruiz, E.M. (2010) Calidad de granos y tortillas de maíces criollos del Altiplano y Valle del Mezquital, México. Revista Fitotecnia Mexicana, 33, 49-56.

[15]   Peña, B.S. (2006) Detection of Fumonisins in Maize (Zea mays L.) by Three Analytical Techniques (HPLC, TLC and ELISA). In: Njapau, H., Sócrates, T., van Egmond, H. and Park, D., Eds., Mycotoxins and Phycotoxins Advances in Determination, Toxicology and Exposure Management, Wageningen Academic Publishers, The Netherlands.

[16]   SAGARPA, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (2002) Norma Mexicana NMX-FF-034/1-SCFI-2002. Productos alimenticios no industrializados-para consumo humano—cereales—maíz blanco para proceso alcalino para tortilla de maíz y productos de maíz nixtamalizado-Especificaciones y métodos de prueba. Dirección General de Normas, SAGARPA, México, D. F. 18 p.

[17]   The Commission of the European Communities (EC) (2006) Commission Regulation (EC) No. 1881/2006 of 19 December 2006 Setting Maximum Levels for Certain Contaminants in Foodstuffs. Official Journal of the European Union, L364, 4-24.

[18]   The Commission of the European Communities (EC) (2010) Commission Regulation (EU) No. 105 2010 Amending Regulation (EC) No. 1881/2006 Setting Maximum Levels for Certain Contaminants in Foodstuffs as Regards Ochratoxin A. Official Journal of the European Union, L035, 7-8.

[19]   EFSA (2006) Opinion of the Scientific Panel on Contaminants in Food Chain on a Request from the Commission Related to Ochratoxin A in Food. EFSA Journal, 365, 1-56.

[20]   De Saeger, S. (2011) Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed. WP Series in Food Science, Technology and Nutrition, Philadelphia.

[21]   Horwitz, W. (2002) Official Methods of Analysis of AOAC International. 17th Edition, AOAC International, Gaithers-bourd.

[22]   Espinoza, P.N., Garrido, R.E. and Pérez, S.M. (2003) Cuantificación de cuatro micotoxinas en el grano de maíz en el estado de Chiapas. Proceedings of I Panamerican Symposium on mycotoxins for industry, México City.

[23]   Flores, O.C.M., Hernández, P.L.B. and Vázquez, M.J. (2006) Mycotoxin Contamination of Grains and Feeds Used in Animal Production in Mexico during 2003. Técnica Pecuaria México, 247-256.

[24]   Cotty, P.P. and Jaime García, R. (2007) Influence of Climate in Aflatoxin Producing Fungi and Aflatoxin Contamination. International Journal of Food Microbiology, 119, 109-115.
http://dx.doi.org/10.1016/j.ijfoodmicro.2007.07.060

[25]   Barberis, M.G., Giaj, G.M. and Reynoso, M.M. (2014) Factors Affecting Distribution and Abundance of Aspergillus Section Nigri in Vineyard Soils from Grape Growing Regions of Argentina. Journal of the Science of Food and Agriculture, 14, 3001-3007.
http://dx.doi.org/10.1002/jsfa.6647

[26]   Schrader, S., Wolfarth, F. and Oldenburg, E. (2013) Biological Control of Soil-Borne Phytopathogenic Fungi and Their Mycotoxins by Soil Fauna. Bulletin UASMV Serie Agriculture, 70, 291-298.

[27]   Driehuis, F., Spanjer, M.C., Scholten, J.M. and Giffel, M.C. (2008) Occurrence of Mycotoxins in Feedstuffs of Dairy Cows and Estimation of Total Dietary Intakes. Journal of Dairy Science, 91, 4261-4271.
http://dx.doi.org/10.3168/jds.2008-1093

[28]   Oviedo, M.S., Barros, G.G., Chulze, S.N. and Ramirez, M.L. (2012) Natural Occurrence of Alternariol and Alternariol Monomethyl Ether in Soya Beans. Mycotoxin Research, 28, 169-174.
http://dx.doi.org/10.1007/s12550-012-0132-0

[29]   Peña, B.S.D. and Carvajal, M.M. (2004) Fumonisins determination in hybrid maize. 1er curso Nacional sobre micotoxinas para la industria, México, D.F.

[30]   Munlvold, G.P. and Mara, J.K. (2002) Laboratory and Growth Chamber Evaluation of Fungicidal Seed Treatments for Maize Seedling Blight Caused by Fusarium Species. Plant Disease, 82, 143-150.

[31]   Galperin, M., Graf, S. and Kenigsbuch, D. (2003) Seed Treatment Prevents Vertical Transmission of Fusarioum moniliforme Making a Significant Contribution to Disease Control. Phytoparasitica, 31, 344-352.
http://dx.doi.org/10.1007/BF02979805

[32]   Pascale, M.N. (2009) Detection Methods for Mycotoxins in Cereal Grains and Cereal Products. Zbornik Matice srpske za prirodne nauke, 117, 15-25.
http://dx.doi.org/10.2298/zmspn0917015p

 
 
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