OJOG  Vol.5 No.11 , September 2015
Effects of Melatonin on Ischemia-Reperfusion Injury in Rat Ovary: Histopathologic and Immunohistochemical Study
ABSTRACT
Aim: To investigate the effect of melatonin on ischemia–reperfusion (I/R) injury in rat ovary. Material and Method: A total of 24 Wistar rats were divided into four groups. The rats were first numbered randomly and then randomly divided into four equal groups: sham, torsion, detorsion and melatonin groups. In group I (n = 6) sham, as well as in group II ovary torsion was not performed and no drug was administered. In group III, 1 hour of ischemia and 2 hours of reperfusion were performed and no drug was given. In group IV, melatonin was dissolved in 1% ethanol just before use. Melatonin was injected 1 hour before torsion to the torsion plus melatonin group. Right ovaries were surgically removed in all groups. Result: Malondialdehyde (MDA) levels were measured. We suggested that administration of melatonin would be in treating ovaries from torsion/detorsion induced damage in humans. The histopathological changes such as vascular congestion, edema, hemorrhage, and follicular degeneration were found to be decreased in the melatonin + ischemia-reperfusion group. Ischemia-reperfusion group: PECAM-I expression is positive in vessel of the stromal area. The follicles and interfolicular area infiltrated with CD68 positive cells were increased in a time of ischemia-reperfusion exposure dependent manner. Conclusions: Melatonin after ischemia-reperfusion CD31 and CD68 expressions is weak, due to the reduction of the inflammatory effects and hemorrhage is thought that melatonin is effective.

Cite this paper
Balsak, D. , Toğrul, C. , Ekinci, C. , Seçkin, K. , Karsli, M. , Ekinci, A. , Tahaoğlu, A. , Bademkiran, H. , Acar, Z. , Tunç, S. , Deveci, E. and Kirman, G. (2015) Effects of Melatonin on Ischemia-Reperfusion Injury in Rat Ovary: Histopathologic and Immunohistochemical Study. Open Journal of Obstetrics and Gynecology, 5, 639-645. doi: 10.4236/ojog.2015.511090.
References
[1]   Halici, Z., Karaca, M., Keles, O.N., Borekci, B., Odabasoglu, F., Suleyman, H., et al. (2008) Protective Effects of Amlodipine on Ischemia-Reperfusion Injury of rat Ovary: Biochemical and Histopathologic Evaluation. Fertility and Sterility, 90, 2408-2415.
http://dx.doi.org/10.1016/j.fertnstert.2007.10.007

[2]   Kara, M., Daglioglu, Y.K., Kuyucu, Y., Tuli, A. and Tap, A. (2012) The Effect of Edaravone on Ischemia-Reperfusion Injury in Rat Ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology, 162, 197-202.
http://dx.doi.org/10.1016/j.ejogrb.2012.02.026

[3]   Abramov, A.Y., Scorziello, A. and Duchen, M.R. (2007) Three Distinct Mechanisms Generate Oxygen Free Radicals in Neurons and Contribute to Cell Death during Anoxia and Reoxygenation. The Journal of Neuroscience, 27, 1129-1138.
http://dx.doi.org/10.1523/JNEUROSCI.4468-06.2007

[4]   Li, R.M. (2002) Jackson Reactive Species Mechanisms of Cellular Hypoxia-Reoxygenation Injury. American Journal of Physiology—Cell Physiology, 282, 227-241.
http://dx.doi.org/10.1152/ajpcell.00112.2001

[5]   Oelsner, G. and Shashar, D. (2006) Adnexial Torsion. Clinical Obstetrics and Gynecology, 40, 459-463.
http://dx.doi.org/10.1097/00003081-200609000-00006

[6]   Oelsner, G., Cohen, S.B., Soriano, D., Adman, D., Mashiach, S. and Carp, H. (2003) Minimal Surgery for the Twisted Ischemic Adnexa Can Preserve Ovarian Function. Human Reproduction, 18, 2599-2602.
http://dx.doi.org/10.1093/humrep/deg498

[7]   Tan, D.X., Chen, L.D., Poeggeller, B., et al. (1993) Melatonin: A Potent Endogenous Hydroxyl Radical Scavenger. Endocrine Journal, 1, 57-60.

[8]   Allegra, M., Reiter, R.J., Tan, D.X., et al. (2003) The Chemistry of Melatonin’s Interaction with Reactive Species. Journal of Pineal Research, 34, 1-10.
http://dx.doi.org/10.1034/j.1600-079X.2003.02112.x

[9]   Leica Biosystems, the Pathology Compony PECAM1 (CD31) Antibody.
http://www.leicabiosystems.com/...antibodies/.../cd31

[10]   Wu, R.J., Van der Hoek, K.H., Ryan, N.K., Norman, R.J. and Robker, R.L. (2004) Macrophage Contributions to Ovarian Function. Human Reproduction, 10, 119-133.
http://dx.doi.org/10.1093/humupd/dmh011

[11]   Gaytan, F., Morales, C., Garcia-Pardo, L., Reymundo, C., Bellido, C. and Sanchez-Criado, J.E. (1998) Macrophages, Cell Proliferation, and Cell Death in the Human Menstrual Corpus Luteum. Biology of Reproduction, 59, 417-425.

[12]   Inderdeo, D.S., Edwards, D.R., Han, V.K. and Khokha, R. (1996) Temporal and Spatial Expression of Tissue Inhibitors of Metalloproteinases during the Natural Ovulatory Cycle of the Mouse. Biology of Reproduction, 55, 498-508.

[13]   Olsner, G., Bider, D. and Goldenberg, M. (1993) Long Term Follow-Up of Twisted Ischemic Adnexa Managed by Detorsion. Fertility and Sterility, 60, 976-979.

[14]   Gordon, J.D., Hopkins, K.L., Jeffrey, R.B. and Guidice, L.C. (1994) Adnexal Torsion: Color Doppler Diagnosis and Laparoscopic Treatment. Fertility and Sterility, 61, 383-385.

[15]   Nisolle, M., Casanas-Roux, F., Qu, J., Motta, P. and Donnez, J. (2000) Histologic and Ultrastructural Evaluation of Fresh and Frozen-Thawed Human Ovarian Xenografts in Nude Mice. Fertility and Sterility, 74, 122-129.
http://dx.doi.org/10.1016/S0015-0282(00)00548-3

[16]   Weissman, A., Gotlieb, L., Colgan, T., Jurisicova, A., Greenblatt, E.M. and Casper, R.F. (1999) Preliminary Experience with Subcutaneous Human Ovarian Cortex Transplantation in the NOD-SCID Mouse. Biology of Reproduction, 60, 1462-1467.
http://dx.doi.org/10.1095/biolreprod60.6.1462

[17]   Liu, Y., Shimizu, I., Omoya, T., Ito, S., Gu, X.S. and Zuo, J. (2002) Protective Effect of Estradiol on Hepatocytic Oxidative Damage. World Journal of Gastroenterology, 8, 363-366.

[18]   Krusche, C.A., Vloet, T.D., Herrler, A., Black, S. and Beier, H.M. (2002) Functional and Structural Regression of the Rabbit Corpus Luteum Is Associated with Altered Luteal Immune Cell Phenotypes and Cytokine Expression Patterns. Histochemistry and Cell Biology, 118, 479-489.

 
 
Top