AS  Vol.6 No.9 , September 2015
Breeding for FHB Resistance via Fusarium Damaged Kernels and Deoxynivalenol Accumulation as Well as Inoculation Methods in Winter Wheat
ABSTRACT
FHB is one of the most destructive diseases of wheat. Resistance testing depends strongly on inoculation methods, and on measured traits. Therefore a four-year (2009-2012) study was performed using spray inoculation + polyethylene (PE) bag cover, spray inoculation + mist irrigation, and spawn method supported by mist irrigation on 40 genotypes, 20 from Hungary and 20 from IFA Tulln, Austria. Each year four isolates were used in artificial inoculations except the spawn method where stalk debris served the inoculum. Visual Fusarium head blight (FHB) scores, Fusarium damaged kernels (FDK) and deoxynivalenol (DON) contamination were checked. 7680 FHB and FDK, as well as 3840 DON analyses served as the background for the statistical evaluation. The most reliable method used was the spray + polyethylene (PE) bag; the other two were significantly poorer being valid for all traits. The FHB scores were the least reliable, whereas the FDK was much more consequent and the DON gave the best results. The FDK gave much better predictions for DON contamination than FHB. The cultivars responses correlated well at different epidemic severities. The presence of the kernel resistance was confirmed and a new trait as extra kernel susceptibility was described. Presence of DON resistance was confirmed again, and extra DON susceptibility was described as a new trait. DON performance varied on the most sensitive cultivar between 0.32 and 143 mg/kg (mean 17.52 mg/kg) and on the most resistant genotype between 0.00 and 18.19 mg/kg (mean 1.87 mg/kg). Correlations between stability and resistance level are r = 0.85 for FHB, 0.78 for FDK, and 0.88 for DON, all at a significance level of p = 0.001. The very close correlation between FDK and DON contamination (r = 0.81, p = 0.001) proves that control of DON contamination needs appropriate resistance. In the breeding program evaluation of FDK is the most important, and then DON will be decided. Variety registration must be updated; otherwise no improvement on the field will occur.

Cite this paper
Mesterházy, A. , Lehoczki-Krsjak, S. , Varga, M. , Szabó-Hevér, Á. , Tóth, B. and Lemmens, M. (2015) Breeding for FHB Resistance via Fusarium Damaged Kernels and Deoxynivalenol Accumulation as Well as Inoculation Methods in Winter Wheat. Agricultural Sciences, 6, 970-1002. doi: 10.4236/as.2015.69094.
References
[1]   Mesterházy, á. (1977) Reaction of winter wheat varieties to four Fusarium species. Journal of Phytopathology, 90, 104-112.
http://dx.doi.org/10.1111/j.1439-0434.1977.tb03224.x

[2]   McMullen, M., Jones, R. and Gallenberg, D. (1997) Scab of Wheat and Barley: A Re-Emerging Disease of Devastating Impact. Plant Disease, 81, 1340-1348.
http://dx.doi.org/10.1094/PDIS.1997.81.12.1340

[3]   McMullen, M., Bergstrom G., De Wolf, E., Dill-Macky, R., Hershman, D., Shaner, G. and Van Sanford, D. (2012) A Unified Effort to Fight an Enemy of Wheat and Barley: Fusarium Head Blight. Plant Disease, 96, 1712-1728.
http://dx.doi.org/10.1094/PDIS-03-12-0291-FE

[4]   Miedaner, T., Wilde , F., Steiner, B., Buerstmayr, H., Korzun, V. and Ebmeyer, E. (2006) Stacking Quantitative Trait loci (QTL) for Fusarium Head Blight Resistance from Non-Adapted Sources in an European Elite Spring Wheat Background and Assessing Their Effects on Deoxynivalenol (DON) Content and Disease Severity. Theoretical and Applied Genetics, 112, 562-569.
http://dx.doi.org/10.1007/s00122-005-0163-4

[5]   Mesterházy, á., Bartók, T. and Lamper, C. (2003) Influence of Wheat Cultivar, Species of Fusarium, and Isolate Aggressiveness on the Efficacy of Fungicides for Control of Fusarium Head Blight. Plant Disease, 87, 1107-1115.
http://dx.doi.org/10.1094/PDIS.2003.87.9.1107

[6]   Mesterházy, á., Tóth, B., Varga, M., Bartók, T., Szabó-Hevér, á., Farády, L. and Lehoczki-Krsjak, S. (2011) Role of Fungicides, of Nozzle Types, and the Resistance Level of Wheat Varieties in the Control of Fusarium Head Blight and Deoxynivalenol. Toxins, 3, 1453-1483.
http://dx.doi.org/10.3390/toxins3111453

[7]   Lehoczki-Krsjak, S., Szabó-Hevér, á., Tóth, B., Kótai, C., Bartók, T., Varga, M., Farády, L. and Mesterházy, á. (2010) Prevention of Fusarium mycotoxin Contamination by Breeding and Fungicide Application in Wheat. Food Additives & Contaminants: Part A, 27, 616-628.
http://dx.doi.org/10.1080/19440041003606144

[8]   Lehoczki-Krsjak, S., Varga, M., Szabó-Hevér, á. and Mesterházy, á. (2013) Translocation and Degradation of Tebuconazole and Prothioconazole in Wheat in the Most Fusarium-Susceptible Phenophase. Pest Management Science, 69, 1216-1224.

[9]   Lehoczki-Krsjak, S., Varga, M. and Mesterházy, á. (2015) Distribution of Prothioconazole and Tebuconazole between Wheat Ears and Flag Leaves Following Fungicide Spraying with Different Nozzle Types at Flowering. Pest Management Science, 71, 105-113.
http://dx.doi.org/10.1002/ps.3774

[10]   Paul, P.A., Lipps, P.E., Hershman, D.E., McMullen, M.P., Draper, M.A. and Madden, L.V. (2008) Efficacy of Triazole-Based Fungicides for Fusarium Head Blight and Deoxynivalenol Control in Wheat: A Multivariate Meta-Analysis. Phytopathology, 98, 999-1011.
http://dx.doi.org/10.1094/PHYTO-98-9-0999

[11]   Juroszek, P. and von Tiedemann, A. (2013) Climate Change and Potential Future Risks through Wheat Diseases: A Review. European Journal of Plant Pathology, 136, 21-33.
http://dx.doi.org/10.1007/s10658-012-0144-9

[12]   Buerstmayr, H, Ban, T. and Anderson J.A. (2009) QTL Mapping and Marker-Assisted Selection for Fusarium Head Blight Resistance in Wheat: A Review. Plant Breeding, 128, 1-26.
http://dx.doi.org/10.1111/j.1439-0523.2008.01550.x

[13]   Szabó-Hevér, á., Lehoczki-Krsjak, S., Tóth, B., Purnhauser, L., Buerstmayr, H., Steiner, B. and Mesterházy, á. (2012) Identification and Validation of Fusarium Head Blight and Fusarium Damaged Kernel QTL in the Frontana/Remus DH Mapping Population. Canadian Journal of Plant Pathology, 34, 224-238.
http://dx.doi.org/10.1080/07060661.2012.676571

[14]   Szabó-Hevér, A., Lehoczki-Krsjak, S., Varga, M., Purnhauser, L., Pauk, J., Lantos, C. and Mesterhazy, A. (2014) Differential Influence of QTL Linked to Fusarium Head Blight, Fusarium-Damaged Kernel, Deoxynivalenol Contents and Associated Morphological Traits in a Frontana-Derived Wheat Population. Euphytica, 200, 9-26.
http://dx.doi.org/10.1007/s10681-014-1229-7

[15]   Draeger, R., Gosman, N., Steed, A., Chandler, E., Thomsett, M., Srinivasachary, Schondelmaier, J., Buerstmayr, H., Lemmens, M., Schmolke, M., Mesterházy, A. and Nicholson P. (2007) Identification of QTLs for Resistance to Fusarium Head Blight, DON Accumulation and Associated Traits in the Winter Wheat Variety Arina. Theoretical and Applied Genetics, 115, 617-625.
http://dx.doi.org/10.1007/s00122-007-0592-3

[16]   Paillard, S., Schnurbusch, T., Tiwari, R., Messmer, M., Winzeler, M., Keller, B. and Schachermayr, G. (2004) QTL Analysis of Resistance to Fusarium Head Blight in Swiss Winter Wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 323-332.
http://dx.doi.org/10.1007/s00122-004-1628-6

[17]   Semagn, K., BjØrnstad, A., Skinnes, H., MarØy, A.G., Tarkegne, Y. and William, M. (2006) Distribution of DArT, AFLP, and SSR Markers in a Genetic Linkage Map of a Doubled-Haploid Hexaploid Wheat Population. Genome, 49, 545-555.
http://dx.doi.org/10.1139/G06-002

[18]   Anderson, J.A., Stack, R.W., Liu, S., Waldron, B.L., Fjeld, A.D., Coyne, C., Moreno-Sevilla, B., Fetch, J.M., Song, Q.J., Cregan, P.B. and Frohberg, R.C. (2001) DNA Markers for Fusarium Head Blight Resistance QTLs Its Two Wheat Populations. Theoretical and Applied Genetics, 102, 1164-1168.
http://dx.doi.org/10.1007/s001220000509

[19]   Buerstmayr, H., Lemmens, M., Hartl, L., Doldi, L., Steiner, B., Stierschneider, M. and Ruckenbauer, P. (2002) Molecular Mapping of QTLs for Fusarium Head Blight Resistance in Spring Wheat. I. Resistance to Fungal Spread (Type II Resistance). Theoretical and Applied Genetics, 104, 84-91.
http://dx.doi.org/10.1007/s001220200009

[20]   Buerstmayr, H., Steiner, B., Hartl, L., Griesser, M., Angerer, N., Lengauer, D., Miedaner, T., Schneider, B. and Lemmens, M. (2003) Molecular Mapping of QTLs for Fusarium Head Blight Resistance in Spring Wheat. II. Resistance to Fungal Penetration and Spread. Theoretical and Applied Genetics, 107, 503-508.
http://dx.doi.org/10.1007/s00122-003-1272-6

[21]   Mesterházy, á., Buerstmayr, H., Tóth, B., Lehoczki-Krsjak, S., Szabó-Hevér, á. and Lemmens, M. (2007) An Improved Strategy for Breeding FHB Resistant Wheat Must Include Type I Resistance. In: Clear, R., Ed., Proc. of the 5th Canadian Workshop on Fusarium Head Blight, Delta Winnipeg, 27-30 November 2007, 51-66.

[22]   Liu, Z.Z. and Wang, Z.Y. (1991) Improved Resistance in China: Sources of Resistance and Problems. In: Saunders, D.A., Ed., Wheat for the Nontraditional, Warm Areas, CIMMYT, Mexico D.F., 178-188.

[23]   del Blanco, I.A., Frohberg, R.C., Stack, R.W., Berzonsky, W.A. and Kianian, S.F. (2003) Detection of QTL Linked to Fusarium Head Blight Resistance in Sumai 3-Derived North Dakota Bread Wheat Lines. Theoretical and Applied Genetics, 106, 1027-1031.

[24]   Mesterházy, á. (1995) Types and Components of Resistance against Fusarium Head Blight of Wheat. Plant Breeding, 114, 377-386.
http://dx.doi.org/10.1111/j.1439-0523.1995.tb00816.x

[25]   Mesterházy, á., Bartók, T., Mirocha, C.M. and Komoróczy, R. (1999) Nature of Resistance of Wheat to Fusarium Head Blight and Deoxynivalenol Contamination and Their Consequences for Breeding. Plant Breeding, 118, 97-110.
http://dx.doi.org/10.1046/j.1439-0523.1999.118002097.x

[26]   Bai, G.-H., Plattner, R., Desjardins, A., Kolb, F. and McIntosh, R.A. (2001) Resistance to Fusarium Head Blight and Deoxynivalenol Accumulation in Wheat. Plant Breeding, 120, 1-6.
http://dx.doi.org/10.1046/j.1439-0523.2001.00562.x

[27]   Lemmens, M., Scholz, U., Berthiller, F., Dall’Asta, C., Koutnik, A., Schuhmacher, R., Adam, G., Buerstmayr, H., Mesterhazy, A., Krska, R. and Ruckenbauer, P. (2005) The Ability to Detoxify the Mycotoxin Deoxynivalenol Co-Localizes with a Major QTL for Fusarium Head Blight Resistance in Wheat. Molecular Plant-Microbe Interactions, 18, 1318-1324.
http://dx.doi.org/10.1094/MPMI-18-1318

[28]   Cossette, F. and Miller, J.D. (1995) Phytotoxic Effect of Deoxynivalenol and Gibberella Ear Rot Resistance of Corn. Natural Toxins, 3, 383-388.
http://dx.doi.org/10.1002/nt.2620030510

[29]   Miller, J.D. and Arnison, P.G. (1986) Degradation of Deoxynivalenol by Suspension Cultures of the Fusarium Head Blight Resistant Wheat Cultivar Frontana. Canadian Journal of Plant Pathology, 8, 147-150.
http://dx.doi.org/10.1080/07060668609501818

[30]   Miller, J.D., Young, J. and Sampson, D.R. (1985) Deoxynivalenol and Fusarium Head Blight Resistance in Spring Cereals. Journal of Phytopathology, 113, 359-367.
http://dx.doi.org/10.1111/j.1439-0434.1985.tb04837.x

[31]   Snijders, C.H.A. (1994) Breeding for Resistance to Fusarium in Wheat and Maize. In: Miller, J.D. and Trenholm, H.L., Eds., Mycotoxins in Grain Compounds Other Than Aflatoxin, Eagan Press, St. Paul, 37-58.

[32]   Mesterházy, á. (2003) Breeding Wheat for Fusarium Head Blight Resistance in Europe. In: Leonard, K.J. and Bushnell, W.R., Eds., Fusarium Head Blight of Wheat and Barley, APS Press, St. Paul, 211-240.

[33]   Mesterházy, á., Bartók, T., Kászonyi, G., Varga, M., Tóth, B. and Varga, J. (2005) Common Resistance to Different Fusarium spp. Causing Fusarium Head Blight in Wheat. European Journal of Plant Pathology, 112, 267-281.
http://dx.doi.org/10.1007/s10658-005-2853-9

[34]   Zwart, R.S., Muylle, H., van Bockstaele, E. and Roldán-Ruiz, I. (2008) Evaluation of Genetic Diversity of Fusarium Head Blight Resistance in European Winter Wheat. Theoretical and Applied Genetics, 117, 813-828.
http://dx.doi.org/10.1007/s00122-008-0822-3

[35]   Dill-Macky, R. (2003) Inoculation Methods and Evaluation of Fusarium Head Blight Resistance in Wheat. In: Leonard, K.J. and Bushnell, W.R., Eds., Fusarium Head Blight in Wheat and Barley, APS Press, St. Paul, 184-210.

[36]   Schroeder, H.W. and Christensen, J.J. (1963) Factors Affecting Resistance of Wheat to Scab Caused by Gibberella zeae. Phytopathology, 53, 831-838.

[37]   Lemmens, M., Buerstmayr, H. and Ruckenbauer, P. (1993) Variation in Fusarium Head Blight Susceptibility of International and Austrian Wheat Breeding Material. Die Bodenkultur, 44, 65-78.

[38]   Lemmens, M., Krska, R., Buerstmayr, H., Josephs, R., Schuhmacher, R., Grausgruber, H. and Ruckenbauer, P. (2003) Fusarium Head Blight Reactions and Accumulation of Deoxynivalenol, Moniliformin and Zearalenone in Wheat Grains. Cereal Research Communications, 31, 407-414.

[39]   Lemmens, M., Buerstmayr, H., Krska, R., Schuhmacher, R., Grausgruber, H. and Ruckenbauer, P. (2004) The Effect of Inoculation Treatment and Long-Term Application of Moisture on Fusarium Head Blight Symptoms and Deoxynivalenol Contamination in Wheat Grains. European Journal of Plant Pathology, 110, 299-308.
http://dx.doi.org/10.1023/B:EJPP.0000019801.89902.2a

[40]   Miedaner, T. (1997) Breeding Wheat and Rye for Resistance to Fusarium Diseases. Plant Breeding, 116, 201-220.
http://dx.doi.org/10.1111/j.1439-0523.1997.tb00985.x

[41]   Ma, H.X., Zhanga, K.M., Gaoa, L., Baib, G.H., Chena, H.G., Caia, Z.X. and Lua, W.Z. (2006) Quantitative Trait Loci for Resistance to Fusarium Head Blight and Deoxynivalenol Accumulation in Wangshuibai Wheat under Field Conditions. Plant Pathology, 55,739-745.
http://dx.doi.org/10.1111/j.1365-3059.2006.01447.x

[42]   Jin, F., Dadong, Z., Bockus, W., Baenziger, P.S., Carver, B. and Bai, G.-H. (2013) Fusarium Head Blight Resistance in U.S. Winter Wheat Cultivars and Elite Breeding Lines. Crop Science, 53, 2006-2013.
http://dx.doi.org/10.2135/cropsci2012.09.0531

[43]   Mesterházy, á. (1977) The Effect of Inoculation Method on the Expression of Symptoms Caused by Fusarium graminearum Schwabe on Wheat in Seedling Stage. Current Topics in Plant Pathology, 1975, issued 1977, Akad. Kiadó, 223-232.

[44]   Mesterházy, á. (2002) Role of Deoxynivalenol in Aggressiveness of Fusarium graminearum and F. culmorum and in Resistance to Fusarium Head Blight. European Journal of Plant Pathology, 108, 675-684.
http://dx.doi.org/10.1023/A:1020631114063

[45]   van Eeuwijk, E.A., Mesterhazy, A., Kling, Ch.I., Ruckenbauer, P., Saur, L., Buerstmayr, H., Lemmens, M., Keizer, L.C.P., Maurin, N. and Snijders, C.H.A. (1995) Assessing Non-Specificity of Resistance of Fusarium culmorum, F. graminearum and F. nivale using a Multiplicative Model for Interaction. Theoretical and Applied Genetics, 90, 221-228.
http://dx.doi.org/10.1007/BF00222205

[46]   Snijders, C.H.A. (1990) Genetic-Variation for Resistance to Fusarium Head Blight in Bread Wheat. Euphytica, 50, 171-179.
http://dx.doi.org/10.1007/BF00023642

[47]   Brown-Guedira, G., Griffey, C., Kolf, F., McKendry, A., Murphy, J.P. and van Sanford, D. (2008) Breeding FHB-Resistant Soft Winter Wheat: Progress and Prospects. Cereal Research Communications, 36, 31-36.
http://dx.doi.org/10.1556/crc.36.2008.suppl.b.5

[48]   Martinez-Espinoza, A., Ethredge, R., Youmans, V., John, B. and Buck, J. (2014) Identification and Control of Fusarium Head Blight (Scab) of Wheat in Georgia.
http://extension.uga.edu/publications/files/pdf/C%201066_1.PDF

[49]   Mesterházy, á. (1984) A Laboratory Method to Predict Pathogenicity of Fusarium graminearum in Field and Resistance to Scab. Acta Phytopathologica Academiae Scientiarum Hungaricae, 19, 205-218.

[50]   Atanasoff, D. (1920) Fusarium Blight (Scab) of Wheat and Other Cereals. Journal of Agricultural Research, 20, 1-32.

[51]   Rudd, J.C., Horsley, R.D., McKendry, A.L. and Elias, E.M. (2001) Host Plant Resistance Genes for Fusarium Head Blight: Sources, Mechanisms, and Utility in Conventional Breeding Systems. Crop Science, 41, 620-627.
http://dx.doi.org/10.2135/cropsci2001.413620x

[52]   Stack, R.W., Frohberg, R.C. and Casper, H. (1997) Reaction of Spring Wheats Incorporating Sumai#3-Derived Resistance to Inoculation with Several Fusarium Species. Cereal Research Communications, 25, 667-671.

[53]   Mesterházy, á. (1985) Effect of Seed Production Area on the Seedling Resistance of Wheat to Fusarium Seedling Blight. Agronomie, 5, 491-497.
http://dx.doi.org/10.1051/agro:19850604

[54]   Grausgruber, H., Lemmens, M., Bürstmayr, H. and Ruckenbauer, P. (1995) Evaluation of Inoculation Methods for Testing Fusarium Head Blight Resistance of Winter Wheat on Single Plant Basis. Bodenkultur, 46, 39-49.

[55]   de Villiers, C.I.P. (2009) A Comparison of Screening Techniques for Fusarium Head Blight of Wheat in South Africa. MS Thesis, University of the Free State, Bloemfontein, 108 p.

[56]   Thomson, C.J. (2010) Evaluation of an Inoculation Method and Quantitative Trait Loci for Fusarium Head Blight Resistance in Wheat. MS Thesis, University of Illinois at Urbana-Champaign, Champaign-Urbana, 108 p.

[57]   Jalaluddin, M.D. and Harrison, S.A. (1993) Repeatability of Stability Estimators for Grain Yield in Wheat. Crop Science, 33, 720-725.
http://dx.doi.org/10.2135/cropsci1993.0011183X003300040017x

[58]   Eberhart, S.A. and Russel, W.A. (1966) Stability Parameters for Comparing Parameters. Crop Science, 6, 36-40.
http://dx.doi.org/10.2135/cropsci1966.0011183X000600010011x

[59]   Sváb, J. (1981) Biometriai Módszerek a Kutatásban (Methods for Biometrics in Research). 3rd Edition, Mezögazdasági Kiadó (Agr. Publ. House), Budapest, 1-557.

[60]   Weber, E. (1967) Grundriss der BiologischenStatistik (Fundaments of the Biological Statistics). VEB Fisher Verlag, Jena.

[61]   Holzapfel, J., Voss, H.-H., Miedaner, T., Korzun, V., Häberle, J., Schweizer, G., Mohler, V., Zimmermann, G. and Hartl, L. (2008) Inheritance of Resistance to Fusarium Head Blight in Three European Winter Wheat Populations. Theoretical and Applied Genetics, 117, 1119-1128.
http://dx.doi.org/10.1007/s00122-008-0850-z

[62]   Kubo, K., Kawada, N., Nakajima, T., Hirayae, K. and Fujita, M. (2014) Field Evaluation of Resistance to Kernel Infection and Mycotoxin Accumulation Caused by Fusarium Head Blight in Western Japanese Wheat (Triticum aestivum L.) Cultivars. Euphytica, 200, 81-93.
http://dx.doi.org/10.1007/s10681-014-1148-7

[63]   Gärtner, B.H., Munich, M. and Mascher, F.N. (2008) Characterization of Kernel Resistance against Fusarium Infection in Spring Wheat by Baking Quality and Mycotoxin Assessments. European Journal of Plant Pathology, 120, 61-68.
http://dx.doi.org/10.1007/s10658-007-9198-5

[64]   Sneller, C., Guttieri, M., Paul, P., Costa, J. and Jackwood, R. (2012) Variation for Resistance to Kernel Infection and Toxin Accumulation in Winter Wheat Infected with Fusarium graminearum. Phytopathology, 102, 306-314.
http://dx.doi.org/10.1094/PHYTO-05-11-0143

[65]   Anonymous (2015) Fusarium Head Blight. Government of Saskatchewan.
http://www.agriculture.gov.sk.ca/fusarium-head-blight

[66]   Wegulo, S., Jackson, T.A., Baenziger, P.S., Carlson, M.P. and Nopsa, J.H. (2008) Fusarium Head Blight of Wheat. University of Nebraska Lincoln, Extension.
http://ianrpubs.unl.edu/live/ec1896/build/ec1896.pdf

[67]   Miller, J.D. and Melvin, A.E. (1997) Toxic Effects of Deoxynivalenol on Ribosomes and Tissues of the Spring Wheat Cultivars Frontana and Casavant. Natural Toxins, 5, 234-237.
http://dx.doi.org/10.1002/(SICI)1522-7189(1997)5:6<234::AID-NT3>3.0.CO;2-Q

[68]   Wilde, F., Korzun, V., Ebmeyer, E., Geiger, H.H. and Miedaner, T. (2007) Comparison of Phenotypic and Marker-Based Selection for Fusarium Head Blight Resistance and DON Content in Spring Wheat. Molecular Breeding, 19, 357-370.
http://dx.doi.org/10.1007/s11032-006-9067-5

[69]   Wilde, F., Schön, C.C., Korzun, V., Ebmeyer, E., Schmolke, M., Hartl, L. and Miedaner T. (2008) Marker-Based Introduction of Three Quantitative-Trait Loci Conferring Resistance to Fusarium Head Blight into an Independent Elite Winter Wheat Breeding Population. Theoretical and Applied Genetics, 117, 29-35.
http://dx.doi.org/10.1007/s00122-008-0749-8

[70]   Yang, Z. (1994) Breeding Resistance to Fusarium Head Blight of Wheat in the Mid- to Lower Yangtze River Valley of China. CIMMYT Wheat Special Report No. 27, 16 p.

[71]   Steiner, B., Lemmens, M., Griesser, M., Scholz, U., Schondelmaier, J. and Buerstmayr, H. (2004) Molecular Mapping of Resistance to Fusarium Head Blight in the Spring Wheat Cultivar Frontana. Theoretical and Applied Genetics, 109, 215-224.
http://dx.doi.org/10.1007/s00122-004-1620-1

[72]   Tóth, B., Kászonyi, G., Bartók, T., Varga, J. and Mesterházy, á. (2008) Common Resistance of Wheat to Members of the Fusarium graminearum Species Complex and F. culmorum. Plant Breeding, 127, 1-8.
http://dx.doi.org/10.1111/j.1439-0523.2008.01412.x

[73]   Mesterházy, á. (1997) Methodology of Resistance Testing and Breeding against Fusarium Head Blight in Wheat and Results of Selection. Cereal Research Communications, 25, 631-637.

 
 
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