Several nutrition and food ingredients are supposed to have beneficial effects, but precise cell biological mechanism has not been elucidated. Among food ingredients, polyphenols such as soy bean isoflavon genistein and wine resveratrol have been reported to have effects on lipid metabolism and cardiovacular diseases (1). In order to elucidate the effect of genistein on obesity, we cultured adipocyte and observed of genisten to lipid accumulation in cells. Triglyceride accumulation was suppressed by genistein when it was added at the time of differentiation but not when added after differentiation. Genistein is considered to suppress lipid accumulation by suppressing the differtiation of adipocytes.
 Moriguchi, E.H., Moriguchi, Y. and Yamori, Y. (2004) Impact of Diet on the Cardiovascular Risk Profile of Japanese Immigrants Living in Brazil: Contributions of World Health Organization CARDIAC and MONALISA Studies. Clinical and Experimental Pharmacology and Physiology, 31, S5-S7. http://dx.doi.org/10.1111/j.1440-1681.2004.04119.x
 Murosaki, S., Lee, T.R., Muroyama, K., Shin, E.S., Cho, S.Y., Yamamoto, Y. and Lee, S.J. (2007) A Combination of Caffeine, Soy Isoflavones, and L-Carnitine Enhances both Lipolysis and Fatty Acid Oxidation in 3T3-L1 and HepG2 Cells in Vitro and in KK Mice in Vivo. Journal of Nutrition, 137, 2252-2257.
 Cederroth, C.R., Vinciguerra, M., Kühne, F., Madani, R., Doerge, D.R., Visser, T.J., Foti, M., Rohner-Jeanrenaud, F., Vassalli, J.D. and Nef, S. (2007) A Phytoestrogen-Rich Diet Increases Energy Expenditure and Decreases Adiposity in Mice. Environmental Health Perspectives, 115, 1467-1473. http://dx.doi.org/10.1289/ehp.10413