AJPS  Vol.6 No.14 , September 2015
Genetic Diversity of Peanut (Arachis hypogea L.) Cultivars as Revealed by RAPD Markers
Abstract: The objectives of this study were to evaluate the genetic diversity of the peanut accessions using Random Amplified Polymorphic DNA (RAPD) molecular marker and to evaluate RAPD markers to be used in peanut as genetic markers and improve such techniques as suitable strategies for peanut germplasm characterization. Twenty peanut accessions were included in this study and were subjected to RAPD molecular markers analysis. Twenty-seven RAPD primers produced 210 amplification products of which 80 (36.4%) were polymorphic. In conclusion, this study reported a successful fingerprinting of peanut accessions using RAPD markers and demonstrated the usefulness of these markers in estimating the extent of genetic variation in peanut germplasm.
Cite this paper: G. Al-Saghir, M. and G. Abdel-Salam, A. (2015) Genetic Diversity of Peanut (Arachis hypogea L.) Cultivars as Revealed by RAPD Markers. American Journal of Plant Sciences, 6, 2303-2308. doi: 10.4236/ajps.2015.614233.

[1]   Fehr, W.R. (1984) Genetic Contributions to Grain Yields of Five Major Crop Plants. CSSA Spec. Publ. 7. CSSA and ASA Madison, WI.

[2]   Bretting, P.K. and Widrlechner, M.P. (1995) Genetic Markers and Plant Genetic Resource Management. Vol. 13, In: Janick, J., Ed., Plant Breeding Reviews, John Wiley & Sons, Inc., New York, 11-87.

[3]   Matus, I.A. and Hayes, P.M. (2002) Genetic Diversity in Three Groups of Barley Germplasm Assessed by Simple Sequence Repeats. Genome, 45, 1095-1106.

[4]   Weining, S. and Langridge, P. (1991) Identification and Mapping of Polymorphisms in Cereals Based on the Polymerase Chain Reaction. Theoretical and Applied Genetics, 82, 209-216.

[5]   Song, W. and Henry, R.J. (1995) Molecular Analysis of the DNA Polymorphism of Wild Barley (Hordeums pontaneum) Germplasm Using the Polymerase Chain Reaction. Genetic Resources and Crop Evolution, 42, 237-281.

[6]   Welsh, J. and McCeleand, M. (1990) Fingerprinting Genomes Using PCR with Arbitrary Primers. Nuclear Acids Research, 18, 7213-7218.

[7]   Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, A. and Tingey, S.V. (1990) DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nuclear Acids Research, 18, 6531-6535.

[8]   Warburton, M.L. and Bliss, F.A. (1996) Genetic Diversity in Peach (Prunuspersica L. Batch) Revealed by Randomly Amplified Polymorphic DNA (RAPD) Markers and Compared to Inbreeding Coefficients. Journal of American Society of Horticultural Science, 121, 1012-1019.

[9]   SAS Institute Inc. SAS 9.2. Help and Documentation. SAS Institute Inc., Cary, 2002-2009.

[10]   Rohlf, F.J. (1993) Numerical Taxonomy and Multivariate Analysis System NTSys-PC Version 1.80 Exeter Software. New York.

[11]   Lanner-Herrera, C., Gustagsson, M., Falt, A.S. and Bryngelsson, T. (1996) Diversity in Natural Populations of Wild Brassica olereacea as Estimated by Isozyme and RAPD Analysis. Genetic Resources and Crop Evolution, 43, 13-23.

[12]   Russell, J.R., Fuller, J.D., Macaulay, M., Hatz, B.G., Jahoor, A., Powell, W. and Waugh, R. (1997) Direct Comparison of Levels of Genetic Variation among Barley Accessions Detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics, 95, 714-722.

[13]   Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, G., Tingey, S. and Rafalski, A. (1996) The Utility of RFLP, RAPD, AFLP and SSR (Microsatellite) Markers for Germplasm Analysis. Molecular Breeding, 2, 225-238.

[14]   Raina, S.N., Rani, V., Kojima, T., Ogihara, Y., Singh, K.P. and Devarumath, R.M. (2001) RAPD and ISSR Fingerprints as Useful Genetic Markers for Analysis of Genetic Diversity, Varietal Identification, and Phylogenetic Relationships in Peanut (Arachishypogaea) Cultivars and Wild Species. Genome, 44, 763-772.