JCDSA  Vol.5 No.3 , September 2015
Higher Cell Viability and Enhanced Sample Quality Following Laser-Assisted Liposuction versus Mechanical Liposuction
Abstract: Background: Despite the popularity of autologous fat transfer applications, high resorption rates, and consequential volume loss, have been reported. Viable adipocyte content has been defined as a key determinant of fat transfer longevity. Moreover, traces of blood, free oil fat and fibrotic tissue accelerate adipocyte degradation. Objective: To compare the effectiveness of a 1470 nm, radial emitting laser-assisted liposection device to a mechanical liposection device in maintaining adipocyte viability in fat tissue harvests. Methods: Bilateral subcutaneous adipose tissue samples were harvested from ten female patients. Fat was harvested from one side using the LipoLife laser-assisted liposuction device and from the other side with a Byron mechanical aspirator. Samples were visually analyzed and blood:fat ratios and cell viability were determined. Results: Laser-harvested samples separated into two distinct phases, with a negligible blood phase at the bottom (1.1%) and a significant adipose phase at the top (98.9%), containing small, uniform-sized cells, of which 95.7% ± 2.7% proved viable. Mechanically harvested samples separated into blood (18%), adipose (60%) and lipid (22%) phases. The adipose phase contained significant amounts of connective tissue, large adipose tissue fragments, large oil droplets and a mean 79.7% ± 18.3% viable adipocytes. Conclusions: Laser liposuctioning was superior to mechanical liposuctioning, providing both higher cell viability and enhanced sample quality. The 1470 nm diode laser bears the potential of improving long-term clinical outcomes of fat transfer procedures. Improved purity of the harvested sample and heightened preadipocyte content are projected to provide for extended graft longevity.
Cite this paper: Levenberg, A. , Scheinowitz, M. and Sharabani-Yosef, O. (2015) Higher Cell Viability and Enhanced Sample Quality Following Laser-Assisted Liposuction versus Mechanical Liposuction. Journal of Cosmetics, Dermatological Sciences and Applications, 5, 238-245. doi: 10.4236/jcdsa.2015.53029.


[2]   Atiyeh, B., Costagliola, M., Illouz, Y.G., et al. (2015) Functional and Therapeutic Indications of Liposuction: Personal Experience and Review of the Literature. Annals of Plastic Surgery.

[3]   Banyard, D.A., Salibian, A.A., Widgerow, A.D. and Evans, G.R. (2015) Implications for Human Adipose-Derived Stem Cells in Plastic Surgery. Journal of Cellular and Molecular Medicine, 19, 21-30.

[4]   Peer, L.A. (1950) Loss of Weight and Volume in Human Fat Grafts. Plastic and Reconstructive Surgery, 5, 217.

[5]   Etzkorn, J.R., Divine, J.M., Lopez, J.J., et al. (2011) Autologous Fat Transfer: Techniques, Indications and Future Investigation. Cosmetic Dermatology, 24, 470-476.

[6]   Kaufman, M.R., Miller, T.A., Huang, C., et al. (2007) Autologous Fat Transfer for Facial Recontouring: Is There Science behind the Art? Plastic and Reconstructive Surgery, 119, 2287-2296.

[7]   Fournier, P.F. (2000) Fat Grafting: My Technique. Dermatologic Surgery, 26, 1117-1128.

[8]   Peer, L.A. (1956) The Neglected Free Fat Graft, Its Behavior and Clinical Use. American Journal of Surgery, 92, 40-44.

[9]   Fagrell, D., Enestrom, S., Berggren, A., et al. (1996) Fat Cylinder Transplantation: An Experimental Comparative Study of Three Different Kinds of Fat Transplants. Plastic and Reconstructive Surgery, 98, 90-96, 97-98.

[10]   Coleman, S.R. (1995) Long-Term Survival of Fat Transplants: Controlled Demonstrations. Aesthetic Plastic Surgery, 19, 421-425.

[11]   Sommer, B. and Sattler, G. (2000) Current Concepts of Fat Graft Survival: Histology of Aspirated Adipose Tissue and Review of the Literature. Dermatologic Surgery, 26, 1159-1166.

[12]   Har-Shai, Y., Lindenbaum, E., Ben-Itzhak, O., et al. (1996) Large Liponecrotic Pseudocyst Formation Following Cheek Augmentation by Fat Injection. Aesthetic Plastic Surgery, 20, 417-419.

[13]   Carpaneda, C.A. (1996) Study of Aspirated Adipose Tissue. Aesthetic Plastic Surgery, 20, 399-402.

[14]   Heymans, O., Castus, P., Grandjean, F.X., et al. (2006) Liposuction: Review of the Techniques, Innovations and Applications. Acta Chirurgica Belgica, 106, 647-653.

[15]   Prado, A., Andrades, P., Danilla, S., et al. (2006) A Prospective, Randomized, Double-Blind, Controlled Clinical Trial Comparing Laser-Assisted Lipoplasty with Suction-Assisted Lipoplasty. Plastic and Reconstructive Surgery, 118, 1032-1045.

[16]   Abdelaal, M.M. and Aboelatta, Y.A. (2014) Comparison of Blood Loss in Laser Lipolysis vs Traditional Liposuction. Aesthetic Surgery Journal, 34, 907-912.

[17]   Badin, A.Z., Moraes, L.M., Gondek, L., et al. (2002) Laser Lipolysis: Flaccidity under Control. Aesthetic Plastic Surgery, 26, 335-339.

[18]   Goldman, A. (2006) Submental Nd:Yag Laser-Assisted Liposuction. Lasers in Surgery and Medicine, 38, 181-184.

[19]   Yoshimura, K., Shigeura, T., Matsumoto, D., et al. (2006) Characterization of Freshly Isolated and Cultured Cells Derived from the Fatty and Fluid Portions of Liposuction Aspirates. Journal of Cellular Physiology, 208, 64-76.