Back
 FNS  Vol.6 No.12 , September 2015
Probiotic Effect of Lebanese Darfiyeh Cheese: A Randomized Case Control Prospective Study in the Elderly
Abstract: A randomized case control prospective method was adopted in this study in order to assess the health benefit associated with the Darfiyeh cheese, a traditional Lebanese raw goat milk cheese ripened in goat skin. 21 participants were recruited and divided into 2 groups: test group (n = 10) and control group (n = 11). Venous blood and stools were collected at day 14, 21 and day 0 and 21 respectively. Flow cytometry was performed on blood sample. Fecal samples were plated on Plate Count Agar (PCA) and de Man, Rogosa and Sharpe Agar (MRS) agar in order to monitor any alteration in the gastrointestinal tract (GIT). At day 24, a significant increase by 27.67% (p = 0.043) in the bacterial count was seen on the MRS agar for the test group in comparison to no change in the total bacterial count. At day 14 a significant drop (p = 0.026) in CD3+/CD4+ population in the test group (35.67 vs 42.87), and a non-significant drop (p = 0.818) in CD8+ (24.76 vs 28.28) is seen when compared to control group. At day 24, no significant change (p = 0.20) in the CD3+/CD4+ population in comparison between both groups and no change in CD8+ or Geo means in both groups. In conclusion, an alteration in the GIT microflora could be hinted at by the lactobacilli bacterium, which was not reflected on the total bacterial count.
Cite this paper: Saber, T. , Irani, J. , Afif, C. , Bassil, N. , Estephan, E. , Serhan, M. and Bassil, M. (2015) Probiotic Effect of Lebanese Darfiyeh Cheese: A Randomized Case Control Prospective Study in the Elderly. Food and Nutrition Sciences, 6, 1086-1094. doi: 10.4236/fns.2015.612113.
References

[1]   Savijoki, K., Ingmer, H. and Varmanen, P. (2006) Proteolytic Systems of Lactic Acid Bacteria. Applied Microbiology and Biotechnology, 71, 394-406.
http;//dx.doi.org/10.1007/s00253-006-0427-1

[2]   Casalta, E. and Montel, M.C. (2008) Safety Assessment of Dairy Microorganisms: The Lactococcus genus. International Journal of Food Microbiology, 126, 271-273.
http;//dx.doi.org/10.1016/j.ijfoodmicro.2007.08.013

[3]   Koretz, R.L. (2009) Probiotics, Critical illness, and Methodologic Bias. Nutrition in Clinical Practice, 24, 45-49.
http;//dx.doi.org/10.1177/0884533608329296

[4]   Han, W., Mercenier, A., Ait-Belgnaoui, A., Pavan, S., Lamine, F., van Swarm, I.I., et al. (2006) Improvement of an Experimental Colitis in Rats by Lactic Acid Bacteria Producing Superoxide Dismutase. Inflammatory Bowel Disease, 12, 1044-1052.
http;//dx.doi.org/10.1097/01.mib.0000235101.09231.9e

[5]   Kaushik, J.K., Kumar, A., Duary, R.K., Mohanty, A.K., Grover, S. and Batish, V.K. (2009) Functional and Probiotic Attributes of an Indigenous Isolate of Lactobacillus plantarum. PLoS One, 4, 8099.
http;//dx.doi.org/10.1371/journal.pone.0008099

[6]   Pavan, S., Desreumaux, P. and Mercenier, A. (2003) Use of Mouse Models to Evaluate the Persistence, Safety, and Immune Modulation Capacities of Lactic Acid Bacteria. Clinical and Diagnostic Laboratory Immunology, 10, 696-701.
http;//dx.doi.org/10.1128/cdli.10.4.696-701.2003

[7]   Bremner, A.R. and Beattie, R.M. (2002) Therapy of Crohn’s Disease in Childhood. Expert Opinion in Pharmacotherapy, 3, 809-825.
http;//dx.doi.org/10.1517/14656566.3.7.809

[8]   Pillai, A. and Nelson, R. (2008) Probiotics for Treatment of Clostridium difficile-Associated Colitis in Adults. Cochrane Database of Systematic Reviews, 1, Article ID: CD004611.
http;//dx.doi.org/10.1002/14651858.CD004611.pub2

[9]   Hirose, Y., Murosaki, S., Yamamoto, Y., Yoshikai, Y. and Tsuru, T. (2006) Daily Intake of Heat-Killed Lactobacillus plantarum L-137 Augments Acquired Immunity in Healthy Adults. Journal of Nutrition, 136, 3069-3073.

[10]   Serhan, M., Linder, M., Hosri, C. and Fanni, J. (2010) Changes in Proteolysis and Volatile Fraction during Ripening of Darfiyeh, a Lebanese Artisanal Raw Goat’s Milk Cheese. Small Ruminant Research, 90, 75-82.
http;//dx.doi.org/10.1016/j.smallrumres.2010.01.008

[11]   Serhan, M., Cailliez-Grimal, C., Borges, F., Revol-Junelles, A.M., Hosri, C. and Fanni, J. (2009) Bacterial Diversity of Darfiyeh, a Lebanese Artisanal Raw Goat’s Milk Cheese. Food Microbiology, 26, 645-652.
http;//dx.doi.org/10.1016/j.fm.2009.04.012

[12]   Garde, S., Avila, M., Arias, R., Gaya, P. and Nunez, M. (2011) Outgrowth Inhibition of Clostridium beijerinckii Spores by a Bacteriocin-Producing Lactic Culture in Ovine Milk Cheese. International Journal of Food Microbiology, 150, 59-65.
http;//dx.doi.org/10.1016/j.ijfoodmicro.2011.07.018

[13]   Adawi, D., Molin, G., Ahrne, S. and Jeppson, B. (2002) Safety of the Probiotics Strain Lactobacillus plantarum DSM 9843 (=Strain 299v) in an Endocarditis Animal Model. Microbial Ecology in Health and Disease, 14, 50-53.
http;//dx.doi.org/10.1080/089106002760002766

[14]   Niedzielin, K., Kordecki, H. and Birkenfeld, B. (2001) A Controlled, Double-Blind, Randomized Study on the Efficacy of Lactobacillus plantarum 299V in Patients with Irritable Bowel Syndrome. European Journal of Gastroenterology and Hepatology, 13, 1143-1147.
http;//dx.doi.org/10.1097/00042737-200110000-00004

[15]   Kimoto, H., Nomura, M., Kobayashi, M., Mizumachi, K. and Okamoto, T. (2003) Survival of Lactococci during Passage through Mouse Digestive Tract. Canadian Journal of Microbiology, 49, 707-711.
http;//dx.doi.org/10.1139/w03-092

[16]   Dal Bello, B., Cocolin, L., Zeppa, G., Field, D., Cotter, P.D. and Hill, C. (2012) Technological Characterization of Bacterio Cin Producing Lactococcus lactis Strains Employed to Control Listeria monocytogenes in Cottage Cheese. International Journal of Food Microbiology, 153, 58-65.
http;//dx.doi.org/10.1016/j.ijfoodmicro.2011.10.016

[17]   Aggarwal, J., Swami, G. and Kumar, M. (2013) Probiotics and Their Effects on Metabolic Diseases: An Update. Journal of Clinical and Diagnostic Research, 7, 173-177.
http;//dx.doi.org/10.7860/jcdr/2012/5004.2701

[18]   Dicks, L.M. and Botes, M. (2010) Probiotic Lactic Acid Bacteria in the Gastro-Intestinal Tract: Health Benefits, Safety and Mode of Action. Beneficial Microbes, 1, 11-29.
http;//dx.doi.org/10.3920/BM2009.0012

[19]   De Vries, M.C., Vaughanb, E.E., Kleerebezema, M. and De Vosa, W.M. (2006) Lactobacillus plantarum-Survival, Functional and Potential Probiotics Properties in the Human Intestinal Tract. International Dairy Journal, 16, 1018-1028.
http;//dx.doi.org/10.1016/j.idairyj.2005.09.003

[20]   Ogier, J.C. and Serror, P. (2008) Safety Assessment of Dairy Microorganisms: The Enterococcus Genus. International Journal of Food Microbiology, 126, 291-301.
http;//dx.doi.org/10.1016/j.ijfoodmicro.2007.08.017

[21]   Mane, J., Pedrosa, E., Loren, V., Gassull, M.A., Espadaler, J., Cune, J., et al. (2011) A Mixture of Lactobacillus plantarum CECT 7315 and CECT 7316 Enhances Systemic Immunity in Elderly Subjects: A Dose-Response, Double-Blind, Placebo-Controlled, Randomized Pilot Trial. Nutricion Hospitalaria, 26, 228-235.

[22]   Chow, S., Hedley, D., Grom, P., Magari, R., Jacobberger, J.W. and Shankey, T.V. (2005) Whole Blood Fixation and Permeabilization Protocol with Red Blood Cell Lysis for Flow Cytometry of Intracellular Phosphorylated Epitopes in Leukocyte Subpopulations. Cytometry A, 67, 4-17.
http;//dx.doi.org/10.1002/cyto.a.20167

[23]   Borchers, A.T., Selmi, C., Meyers, F.J., Keen, C.L. and Gershwin, M.E. (2009) Probiotics and Immunity. Journal of Gastroenterology, 44, 26-46.
http;//dx.doi.org/10.1007/s00535-008-2296-0

[24]   Isolauri, E., Sutas, Y., Kankaanpaa, P., Arvilommi, H. and Salminen, S. (2001) Probiotics: Effects on Immunity. American Journal of Clinical Nutrition, 73, 444S-450S.

[25]   Rizzello, V., Bonaccorsi, I., Dongarra, M.L., Fink, L.N. and Ferlazzo, G. (2011) Role of Natural Killer and Dendritic Cell Crosstalk in Immunomodulation by Commensal Bacteria Probiotics. Journal of Biomedicine and Biotechnology, 2011, Article ID: 473097.
http;//dx.doi.org/10.1155/2011/473097

[26]   Michetti, P., Dorta, G., Wiesel, P.H., Brassart, D., Verdu, E., Herranz, M., et al. (1999) Effect of Whey-Based Culture Supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori Infection in Humans. Digestion, 60, 203-209.
http;//dx.doi.org/10.1159/000007660

[27]   Baharav, E., Mor, F., Halpern, M. and Weinberger, A. (2004) Lactobacillus GG Bacteria Ameliorate Arthritis in Lewis Rats. Journal of Nutrition, 123, 1964-1969.

[28]   Kato, I., Endo-Tanaka, K. and Yokokura, T. (1998) Suppressive Effects of the Oral Administration of Lactobacillus casei on Type II Collagen-Induced Arthritis in DBA/1 Mice. Life Sciences, 63, 635-644.
http;//dx.doi.org/10.1016/S0024-3205(98)00315-4

[29]   Ratajczak, C., Duez, C., Grangette, C., Pochard, P., Tonnel, A.B. and Pestel, J. (2007) Impact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium. Journal of Biomedicine and Biotechnology, 2007, Article ID: 71921.
http;//dx.doi.org/10.1155/2007/71921

[30]   Taylor, A.L., Dunstan, J.A. and Prescott, S.L. (2007) Probiotic Supplementation for the First 6 Months of Life Fails to Reduce the Risk of Atopic Dermatitis and Increases the Risk of Allergen Sensitization in High-Risk Children: A Randomized Controlled Trial. Journal of Allergy and Clinical Immunology, 119, 184-191.
http;//dx.doi.org/10.1016/j.jaci.2006.08.036

 
 
Top