AJPS  Vol.6 No.13 , August 2015
Dark Response of Seedlings Evaluated by Chlorophyll Concentration in Maize Natural Population
Abstract: Chlorophyll, one of the major chloroplast components for photosynthesis, has a positive relationship with the photosynthetic rate. The chlorophyll content is an important assessment parameter in agronomy and plant biology research. This study was conducted to evaluate the natural variation in the chlorophyll content and to determine the differential response of the chlorophyll concentration to dark treatment in a natural population containing 139 maize inbreds. A five-fold higher chlorophyll concentration was measured in the light compared with the dark. Meanwhile, the wide variation in the chlorophyll concentration showed the differential response of the natural maize population to dark. Finally, we identified some inbreds that were highly sensitive to the dark with more than 70% difference between the light and dark treatment, such as Dan598, Zheng29, Zheng35, DH29, and R08, as well as some inbreds that had lower sensitivity to the dark, with less than 35% difference in the chlorophyll content between the light and dark treatment, such as Chuan48-2, 4F1, 303WX, 9642, and LY042.
Cite this paper: Chan, A. , Xu, S. , Guo, D. , Shi, Y. , Li, Y. , Li, Y. , Xue, J. (2015) Dark Response of Seedlings Evaluated by Chlorophyll Concentration in Maize Natural Population. American Journal of Plant Sciences, 6, 2209-2219. doi: 10.4236/ajps.2015.613223.

[1]   Tanaka, A. and Tsuji, H. (1985) Appearance of Chlorophyll-Protein Complexes in Greening Barley Seedlings. Plant & Cell Physiology, 26, 893-902.

[2]   Guo, P.G. and Li, M.Q. (1996) Studies on Photosynthetic Characteristics in Rice Hybrid Progenies and Their Parents I. Chlorophyll Content, Chlorophyll-Protein Complex and Chlorophyll Fluorescence Kinetics. Journal of Tropical and Subtropical Botany, 4, 60-65.

[3]   Guo, P.G., Baum, M., Varshney, R.K., Graner, A., Grando, S. and Ceccarelli, S. (2008) QTLs for Chlorophyll and Chlorophyll Fluorescence Parameters in Barley under Post-Flowering Drought. Euphytica, 163, 203-214.

[4]   Op den Camp, R.G., Przybyla, D., Ochsenbein, C., Laloi, C., Kim, C.C., Danon, A., Wagner, D., Hideg, E., Gobel, C., Feussner, I., Nater, M. and Apel, K. (2003) Rapid Induction of Distinct Stress Responses after the Release of Singlet Oxygen in Arabidopsis. Plant Cell, 15, 2320-2332.

[5]   Ito, H. and Tanaka, A. (1996) Determination of the Activity of Chlorophyll b to Chlorophyll a Conversion during Greening of Etiolated Cucumber Cotyledons by Using Pyrochlorophyllide b. Plant Physiology and Biochemistry, 34, 35-40.

[6]   Rüdiger, W. (2002) Biosynthesis of Chlorophyll b and the Chlorophyll Cycle. Photosynthesis Research, 74, 187-193.

[7]   Barber, J., Morris, E . and Büchel, C. (2000) Revealing the Structure of the Photosystem II Chlorophyll Binding Proteins, CP43 and CP47. Biochimica et Biophysica Acta (BBA)—Bioenergetics, 1459, 239-247.

[8]   Grossman, A.R., Bhaya, D., Apt, K.E. and Kehoe, D.M. (1995) Light Harvesting Complexes in Oxygenic Photosynthesis: Diversity, Control, and Evolution. Annual Review of Genetics, 29, 231-288.

[9]   Lamb, J.J., Eaton-Rye, J.J. and Hohmann-Marriott, M.F. (2012) An LED-Based Fluorometer for Chlorophyll Quantification in the Laboratory and in the Field. Photosynthesis Research, 114, 59-68.

[10]   Kannanngarak, C.G. (1991) The Photosynthetic Apparatus. Academic Press Inc., San Diego.

[11]   Wu, P. and Luo, A.C. (1996) Investigation on Genetic Background of Leaf Chlorophyll Content Variation in Rice under Nitrogen Stressed Condition via Molecular Markers. Acta Genetica Sinica, 23, 431-438.

[12]   Yildirim, E., Turan, M. and GuvenÇ, I. (2008) Effect of Foliar Salicylic Acid Applications on Growth, Chlorophyll, and Mineral Content of Cucumber Grown under Salt Stress. Journal of Plant Nutrition, 31, 593-612.

[13]   Sevengor, S., Yasar, F., Kusvuran, S. and Ellialtioglu, S. (2011) The Effect of Salt Stress on Growth, Chlorophyll Content, Lipid Peroxidation and Antioxidative Enzymes of Pumpkin Seedling. African Journal of Agricultural Research, 6, 4920-4924.

[14]   Avcioglu, R., Demiroglu, G., Khalvati, M.A. and Geren, H. (2003) Effects of Osmotic Pressure on Early Growing Stages of Some Crop Plants II. Proline, Chlorophile Accumulation and Membrane Integrity. Ziraat Fakültesi Dergisi, 40, 9-16.

[15]   Johnston, M. and Onwueme, I.C. (1998) Effect of Shade on Photosynthetic Pigments in the Tropical Root Crops: Yam, Taro, Tannia, Cassava and Sweet Potato. Experimental Agriculture, 34, 301-312.

[16]   Dai, Y.J., Shen, Z.G., Liu, Y., Wang, L.L., Hannaway, D. and Lu, H.F. (2009) Effects of Shade Treatments on the Photosynthetic Capacity, Chlorophyll Fluorescence, and Chlorophyll Content of Tetrastigma hemsleyanum Diels et Gilg. Environmental and Experimental Botany, 65, 177-182.

[17]   Khan, S.R., Rose, R., Haase, D.L. and Sabin, T.E. (2000) Effects of Shade on Morphology, Chlorophyll Concentration and Chlorophyll Fluorescence of Four Pacific Northwest Conifer Species. New Forests, 19, 171-186.

[18]   Demircioglu, N. and Yilmaz, H. (2005) Light Pollution: Problems and Solution Proposals. Ataturk üniversitesi Ziraat Fakültesi Dergisi, 36, 117-123.

[19]   Gunes, A. and Inal, A. (1995) The Effect of Foliar Applied Glucose on the Yield and Chlorophyll Content of Wheat (Triticum aestium L.) Grown at Different Photoperiods. Pamukkale üniversitesi Mühendislik Fakültesi, Mühendislik Bilimleri Dergisi, 1, 69-72.

[20]   Mena-Violante, H.G., Ocampo-Jimenez, O., Dendooven, L., Martinez-Soto, G., Gonzolaz-Castenada, J., Fredt-Davies, J.R., Olalde-Portugal, V. and Arbuscular, V. (2006) Arbuscular Mycorrhizal Fungi Enhance Fruit Growth and Quality of Chile Ancho (Capsicum annuum L. cv San Luis) Plants Exposed to Drought. Mycorrhiza, 16, 261-267.

[21]   Vij, S. and Tyagi, A.K. (2007) Emerging Trends in the Functional Genomics of the Abiotic Stress Response in Crop Plants. Plant Biotechnology Journal, 5, 361-380.

[22]   Aguero, M.V., Barg, M.V., Yommi, A., Camelo, A. and Roura, S.I. (2008) Postharvest Changes in Water Status and Chlorophyll Content of Lettuce (Lactuca sativa L.) and Their Relationship with Overall Visual Quality. Journal of Food Science, 73, 47-55

[23]   Sevik, H., Guney, D., Karakas, H. and Aktar, G. (2012) Change to Amount of Chlorophyll on Leaves Depend on Insolation in Some Landscape Plants. International Journal of Environmental Science, 3, 1057-1064.

[24]   Demirel, K., Genc, L., Camoglu, G. and Asik, S. (2010) Assessment of Water Stress Using Chlorophyll Readings and Leaf Water Content for Watermelon. Journal of Tekirdag Agricultural Faculty, 7, 155-162.

[25]   Kalefetoglu, T. and EkmekÇi, Y. (2005) The Effects of Drought on Plants and Tolerance Mechanisms (Review). Gazi University Journal of Science, 18, 723-740.

[26]   Elkoca, E. (2003) Air Pollution and Its Effects on Plants. Ataturk üniversitesi Ziraat Fakültesi Dergisi, 34, 367-374.

[27]   Tunal, M.M., Çarp1c1, E.B. and Çelik, N. (2012) Effects of Different Nitrogen Rates on Chlorophyll Content, Leaf Area Index and Grain Yield of Some Maize Cultivars. Tarlm Bilimleri Arastlrma Dergisi, 5, 131-133.

[28]   Zavoruev, V.V. and Zavorueva, E.N. (2002) Changes in the Ratio between the Peaks of Red Chlorophyll Fluorescence in Leaves of Populus balsamifera during Vegetation. Doklady Biochemistry and Biophysics, 387, 1-6.

[29]   Gond, V., De Pury, D.G.G., Veroustraete, F. and Ceulemans, R. (2012) Seasonal Variations in Leaf Area Index, Leaf Chlorophyll, and Water Content; Scaling-Up to Estimate fAPAR and Carbon Balance in a Multilayer, Multispecies Temperate Forest. Tree Physiology, 19, 673-679.

[30]   Perks, M.P., Osborne, B.A. and Mitchell, D.T. (2004) Rapid Predictions of Cold Tolerance in Douglas-Fir Seedlings Using Chlorophyll Fluorescence after Freezing. New Forests, 28, 49-62.

[31]   Hua, L.R., Guo, G.P., Micheal, B., Stefania, G. and Salvatore, C. (2006) Evaluation of Chlorophyll Content and Fluorescence Parameters as Indicators of Drought Tolerance in Barley. Agricultural Sciences in China, 5, 751-757.

[32]   Longenberger, P.S., Smith, C.W., Duke, S.E. and McMichael, B.L. (2009) Evaluation of Chlorophyll Fluorescence as a Tool for the Identification of Drought Tolerance in Upland Cotton. Euphytica, 166, 25-33.

[33]   Brestic, M. and Zivcak, M. (2013) Molecular Stress Physiology of Plants. Springer India Inc., New Delhi.

[34]   Ehlert, B. and Hincha, D.K. (2008) Chlorophyll Fluorescence Imaging Accurately Quantifies Freezing Damage and Cold Acclimation Responses in Arabidopsis Leaves. Plant Methods, 4, 1-7.

[35]   Rizza, F., Pagani, D., Stanca, A. and Cattivelli, L. (2001) Use of Chlorophyll Fluorescence to Evaluate the Cold Acclimation and Freezing Tolerance of Winter and Spring Oats. Plant Breeding, 120, 389-396.

[36]   FAO (2009) Global Agriculture towards 2050. Briefing Paper for FAO High-Level Expert Forum on “How to Feed the World 2050”, Rome, 21-23.

[37]   Strable, J. and Scanlon., M.J. (2009) Maize (Zea mays): A Model Organism for Basic and Applied Research in Plant Biology. Cold Spring Harbor Protocols, 2009, Article ID: pdb.emo132.

[38]   Ku, L.X., Zhao, W.M., Zhang, J., Wu, L.C., Wang, C.L.,Wang, P.A., Zhang, W.Q. and Chen,Y.H. (2010) Quantitative Trait Loci Mapping of Leaf Angle and Leaf Orientation Value in Maize (Zea mays L.). Theoretical and Applied Genetics, 121, 951-959

[39]   Li, Q., Yang, X.H., Xu, S.T., Cai, Y., Zhang, D.L., Han, Y.J., Li, L., Zhang, Z.X., Gao, S.B., Li, J.S. and Yan, J.B. (2012) Genome-Wide Association Studies Identified Three Independent Polymorphisms Associated with α-Tocopherol Content in Maize Kernels. PLoS ONE, 7, e36807.

[40]   Arnon, D.I. (1949) Copper Enzymes in Isolated Chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiology, 24, 1-15.

[41]   Zhang, Z.S., Li, G., Ga, H.Y., Zhang, L.T., Yang, C., Liu, P. and Meng, Q.W. (2012) Characterization of Photosynthetic Performance during Senescence in Stay-Green and Quick-Leaf-Senescence Zea mays L. Inbred Lines. PLoS ONE, 7, e42936.

[42]   Zhang, W.Y., Xu, Y.C., Li, W.L., Yang, L., Yue, X., Zhang, X.S. and Zhao, X.Y. (2014) Transcriptional Analyses of Natural Leaf Senescence in Maize. PLoS ONE, 9, e115617.