Early Season Development of Micro/Nano-Morphology and Superhydrophobic Wetting Properties on Aspen Leaf Surfaces

Uwe  Erb; Jared Jennings  Victor; George Christopher  Tranquada

doi:10.4236/ajps.2015.613222

George Christopher Tranquada, Jared Jennings Victor, Uwe Erb^*

Abstract: The rapid growth and early development period of the dual-scale surface topography was studied on the adaxial leaf surfaces of two aspen tree species with non-wetting leaves: the columnar European aspen (Populus tremula “Erecta”) and quaking aspen (Populus tremuloides). Particular attention was focused on the formation of micro- and nano-scale asperities on their cuticles, which was correlated with the development of superhydrophobic wetting behaviour. Measurements of the wetting properties (contact angle and tilt-angle) provided an indication of the degree of hydrophobicity of their cuticles. Scanning electron microscopy and optical profilometry micrographs were used to follow the growth and major morphological changes of micro-scale papillae and nano-scale epicuticular wax (ECW) crystals, which led to a significant improvement in non-wetting behaviour. Both species exhibited syntopism in the form of small and larger nano-scale ECW platelet morphologies. These findings provide additional support for earlier suggestions that due to fluctuations in leaf hydrophobicity throughout the growing season, canopy storage capacity may also vary considerably throughout this time period.

Keywords: Columnar European Aspen Leaves, Development of Superhydrophobic Leaf Surfaces, Epicuticular Wax Morphologies, Nano-Scale Wax Crystals, Quaking Aspen Leaves

Cite this paper: Tranquada, G. , Victor, J. and Erb, U. (2015) Early Season Development of Micro/Nano-Morphology and Superhydrophobic Wetting Properties on Aspen Leaf Surfaces. American Journal of Plant Sciences, 6, 2197-2208. doi: 10.4236/ajps.2015.613222.

References

[1] Barthlott, W. and Neinhuis, C. (1997) Purity of the Sacred Lotus, or Escape from Contamination in Biological Surfaces. Planta, 202, 1-8.
http://dx.doi.org/10.1007/s004250050096

[2] Barthlott, W., Neinhuis, C., Cutler, D., Ditsch, F., Meusel, I., Theisen, I. and Wilhelmi, H. (1998) Classification and Terminology of Plant Epicuticular Waxes. Botanical Journal of the Linnean Society, 126, 237-260.
http://dx.doi.org/10.1111/j.1095-8339.1998.tb02529.x

[3] Bhushan, B. and Jung, Y.C. (2006) Micro- and Nanoscale Characterization of Hydrophobic and Hydrophilic Leaf Surfaces. Nanotechnology, 17, 2758-2772.
http://dx.doi.org/10.1088/0957-4484/17/11/008

[4] Burton, Z. and Bhushan, B. (2006) Surface Characterization and Adhesion and Friction Properties of Hydrophobic Leaf Surfaces. Ultramicroscopy, 106, 709-719.
http://dx.doi.org/10.1016/j.ultramic.2005.10.007

[5] Rosado, B.H.P. and Holder, C.D. (2013) The Significance of Leaf Water Repellency in Ecohydrological Research: A Review. Ecohydrology, 6, 150-161.
http://dx.doi.org/10.1002/eco.1340

[6] Victor, J.J. and Erb, U. (2010) Superhydrophobic Structures on the Basis of Aspen Leaf Design. International Journal of Micro-Nano Scale Transport, 1, 323-333.
http://dx.doi.org/10.1260/1759-3093.1.4.323

[7] Victor, J.J., Facchini, D. and Erb, U. (2012) A Low-Cost Method to Produce Superhydrophobic Polymer Surfaces. Journal of Materials Science, 47, 3690-3697.
http://dx.doi.org/10.1007/s10853-011-6217-x

[8] Tranquada, G.C. and Erb, U. (2014) Morphological Development and Environmental Degradation of Superhydrophobic Aspen and Black Locust Leaf Surfaces. Ecohydrology, 7, 1421-1436.
http://dx.doi.org/10.1002/eco.1468

[9] Aryal, B. and Neuner G. (2010) Leaf Wettability Decreases along an Extreme Altidudinal Gradient. Oecologia, 162, 1-9.
http://dx.doi.org/10.1007/s00442-009-1437-3

[10] Koch, K., Bhushan, B. and Barthlott, W. (2008) Diversity of Structure, Morphology and Wetting of Plant Surfaces. Soft Matter, 4, 1943-1963.
http://dx.doi.org/10.1039/b804854a

[11] Riederer, M. and Schreiber, L. (2001) Protecting against Water Loss: Analysis of the Barrier Properties of Plant Cuticles. Journal of Experimental Botany, 52, 2023-2032.
http://dx.doi.org/10.1093/jexbot/52.363.2023

[12] Stark, R. and Tian, S. (2006) The Cutin Biopolyester Matrix. Annual Plant Reviews, Biology of the Plant Cuticle, 23, 126-144.
http://dx.doi.org/10.1002/9780470988718.ch3

[13] Solga, A., Cerman, Z., Striffler, B.F., Spaeth, M. and Barthlott, W. (2007) The Dream of Staying Clean: Lotus and Biomimetic Surfaces. Bioinspiration and Biomimetics, 2, 126-134.
http://dx.doi.org/10.1088/1748-3182/2/4/S02

[14] Jeffree, C.E., Baker, E.A. and Holloway, P.J. (1975) Ultrastructure and Recrystallization of Plant Epicuticular Waxes. New Phytologist, 75, 539-549.
http://dx.doi.org/10.1111/j.1469-8137.1975.tb01417.x

[15] Jetter, R. and Schaffer, S. (2001) Chemical Composition of the Prunus laurocerasus Leaf Surface. Dynamic Changes of the Epicuticular Wax Film during Leaf Development. Plant Physiology, 126, 1725-1737.
http://dx.doi.org/10.1104/pp.126.4.1725

[16] Neinhuis, C., Koch, K. and Barthlott, W. (2001) Movement and Regeneration of Epicuticular Waxes through Plant Cuticles. Planta, 213, 427-434.
http://dx.doi.org/10.1007/s004250100530

[17] Koch, K. and Ensikat, H.J. (2008) The Hydrophobic Coatings of Plant Surfaces: Epicuticular Wax Crystals and Their Morphologies, Crystallinity and Molecular Self-Assembly. Micron, 39, 759-772.
http://dx.doi.org/10.1016/j.micron.2007.11.010

[18] Holder, C.D. (2013) Effects of Leaf Hydrophobicity and Water Droplet Retention on Canopy Storage Capacity. Ecohydrology, 6, 483-490.
http://dx.doi.org/10.1002/eco.1278

[19] Little, E.L. (2010) National Audobon Society Field Guide to North American Trees, Eastern Region. Chanticleer Press, New York, 323-327.

[20] Stam, B.R., Malechek, J.C., Bartos, D.L., Bowns, J.E. and Godfrey, E.B. (2008) Effect of Conifer Encroachment into Aspen Stands on Understory Biomass. Rangeland Ecology and Management, 61, 93-97.
http://dx.doi.org/10.2111/06-156R2.1

[21] Victor, J.J. and Erb, U. (2013) Influence of Weather Conditions on the Surface Morphology and Wetting Behaviour of Superhydrophobic Quaking Aspen Leaves. American Journal of Plant Sciences, 4, 61-68.
http://dx.doi.org/10.4236/ajps.2013.45A010

[22] Holder, C.D. (2012) The Relationship between Leaf Hydrophobicity, Water Droplet Retention, and Leaf Angle of Common Species in a Semi-Arid Region of the Western United States. Agricultural and Forest Meteorology, 152, 11-16.
http://dx.doi.org/10.1016/j.agrformet.2011.08.005

[23] Hallam, N.D. (1970) Growth and Regeneration of Waxes on the Leaves of Eucalyptus. Planta, 93, 257-268.
http://dx.doi.org/10.1007/BF00387646

[24] Neinhuis, C. and Barthlott, W. (1998) Seasonal Changes of Leaf Structure Contamination in Beech, Oak, and Ginkgo in Relation to Leaf Micromorphology and Wettability. New Phytologist, 138, 91-98.
http://dx.doi.org/10.1046/j.1469-8137.1998.00882.x

[25] Victor, J.J., Facchini, D., Palumbo, G. and Erb, U. (2012b) Biology Inspired Superhydrophobic Surfaces. Advanced Materials Research, 409, 814-819.

[26] ImageJ. http://imagej.nih.gov/ij/

[27] Einspahr, D.W. and Winton, L.L. (1976) Genetics of Quaking Aspen. Aspen Bibliography. Paper 5026.
http://digitalcommons.usu.edu/aspen_bib/5026

[28] Barnes, B.V. (1978) Pollen Abortion in Betula and Populus (Section Leuce). The Michigan Botanist, 17, 167-172.

[29] Worrell, R. (1995) European Aspen (Populus tremula L.): A Review with Particular Reference to Scotland I. Distribution, Ecology and Genetic Variation. Forestry, 68, 93-105.
http://dx.doi.org/10.1093/forestry/68.2.93

[30] Koch, K., Bhushan, B., Ensikat, H.J. and Barthlott, W. (2009) Self-Healing of Voids in the Wax Coating on Plant Surfaces. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 367, 1673-1688.
http://dx.doi.org/10.1098/rsta.2009.0015

[31] Smith, W.K. and McClean, T.M. (1989) Adaptive Relationship between Leaf Water Repellency, Stomatal Distribution, and Gas Exchange. American Journal of Botany, 76, 465-469.
http://dx.doi.org/10.2307/2444617

[32] Pandey, S. and Nagar, P.K. (2003) Patterns of Leaf Surface Wetness in Some Important Medicinal and Aromatic Plants of Western Himalaya. FLORA, 198, 349-357.
http://dx.doi.org/10.1078/0367-2530-00107

[33] Brewer, C.A. and Nunez, C.I. (2007) Patterns of Leaf Wettability along an Extreme Moisture Gradient in Western Patagonia, Argentina. International Journal of Plant Sciences, 168, 555-562.
http://dx.doi.org/10.1086/513468

[34] Holder, C.D. (2011) The Relationship between Leaf Water Repellency and Leaf Traits in Three Distinct Biogeographical Regions. Plant Ecology, 212, 1913-1926.
http://dx.doi.org/10.1007/s11258-011-9963-6