NR  Vol.6 No.8 , August 2015
Characterization of Springtime Coliform Populations at the End Creek Wetland Restoration (Union Co., Oregon, USA): A Three-Year Study
ABSTRACT
In 2005, a 550-acre tract of agricultural land containing two small streams near La Grande, Oregon was registered in the U.S. Federal Wetlands Reserve Program. This designation was part of a plan to reclaim and restore the wetland to its natural state. Initial efforts at the End Creek Restoration Project restored both End Creek and South Fork Willow Creek to a natural course through rechanneling, and several plantings had restored some of the native flora. Since its establishment, the End Creek streams and floodplain have become a reserve for summer steelhead (Oncorhynchus mykiss), a threatened anadromous salmonid, and many migratory birds. The threatened Columbia Spotted Frog has also established itself in some of the ponds. As part of an effort to establish a baseline for water quality, we monitored total springtime coliform and fecal coliform bacteria in three of the End Creek ponds for three years. The results of this study indicate that, throughout any given spring, the numbers of both coliform and fecal coliform bacteria can fluctuate markedly among ponds on any given day, and that in any particular pond the numbers fluctuate from week to week. In addition, our analysis suggests that in early spring, the numbers of these organisms also fluctuate from year to year. The causes of these fluctuations are not well understood, but are expected to reflect both springtime flooding and the migrations of source animals such as waterfowl and cervids. Information gathered from this study will help inform future management activities on the wetland.

Cite this paper
Corsini, J. , Peters, L. , Tarpy, B. , Pak, C. and Antell, K. (2015) Characterization of Springtime Coliform Populations at the End Creek Wetland Restoration (Union Co., Oregon, USA): A Three-Year Study. Natural Resources, 6, 482-490. doi: 10.4236/nr.2015.68046.
References
[1]   Karim, M.R., Manshadi, F.D., Karpiscak, M.M. and Gerba, C.P. (2004) The Persistence and Removal of Enteric Pathogens in Constructed Wetlands. Water Research, 38, 1831-1837. http://dx.doi.org/10.1016/j.watres.2003.12.029

[2]   Graczyk, T.K. and Lucy, F.E. (2007) Quality of Reclaimed Waters: A Public Health Need for Source Tracking of Wastewater-Derived Protozoan Enteropathogens in Engineered Wetlands. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101, 532-533. http://dx.doi.org/10.1016/j.trstmh.2007.02.018

[3]   Cohen, J. and Shuval, H.I. (1973) Coliforms, Fecal Coliforms, and Fecal Streptococci as Indicators of Water Pollution. Water, Air, and Soil Pollution, 2, 85-95. http://dx.doi.org/10.1007/BF00572392

[4]   Hoadley, A.W. (1977) Bacterial Indicators/Health Hazards Associated with Water. ASTM International, 635, 3-59. http://dx.doi.org/10.1520/stp635-eb

[5]   Gordon, D.M. (2001) Geographical Structure and Host Specificity in Bacteria and the Implications for Tracing the Source of Coliform Contamination. Microbiology, 147, 1079-1085.

[6]   Vymazal, J. (2005) Removal of Enteric Bacteria in Constructed Treatment Wetlands with Emergent Macrophytes: A Review. Journal of Environmental Science and Health, 40, 1355-1367. http://dx.doi.org/10.1081/ESE-200055851

[7]   Liwimbi, L., Graves, A.K., Israel, D.W., Heugten, E.V., Robinson, B., Cahoon, C.W. and Lubbers, J.F. (2010) Microbial Source Tracking in a Watershed Dominated by Swine. Water, 2, 587-604. http://dx.doi.org/10.3390/w2030587

[8]   Brunet, N.N. and Westbrook, C.J. (2012) Wetland Drainage in the Canadian Prairies: Nutrient, Salt and Bacteria Characteristics. Agriculture, Ecosystems & Environment, 146, 1-12. http://dx.doi.org/10.1016/j.agee.2011.09.010

[9]   Sauvé, S., Aboulfadl, K., Dorner, S., Payment, P., Deschamps, G. and Prévost, M. (2012) Fecal Coliforms, Caffeine and Carbamazepine in Stormwater Collection Systems in a Large Urban Area. Chemosphere, 86, 118-123. http://dx.doi.org/10.1016/j.chemosphere.2011.09.033

[10]   Eppley, R.W., Carlucci, A.F., Holm-Hansen, O., Kiefer, D., McCarthy, J.J. and Williams, P.M. (1972) Evidence for Eutrophication in the Sea near Southern California Coastal Sewage Outfalls, July, 1970. CalCOFI Report, California Marine Research Communication, 16, 74-83.

[11]   Mallin, M.A., McIver, M.R., Wells, H.A., Parsons, D.C. and Johnson, V.L. (2005) Reversal of Eutrophication Following Sewage Treatment Upgrades in the New River Estuary, North Carolina. Estuaries, 28, 750-760. http://dx.doi.org/10.1007/BF02732912

[12]   Jarvie, H.P., Neal, C. and Withers, P.J. (2006) Sewage-Effluent Phosphorus: A Greater Risk to River Eutrophication than Agricultural Phosphorus? Science of the Total Environment, 360, 246-253. http://dx.doi.org/10.1016/j.scitotenv.2005.08.038

[13]   Dorsey, J.H., Carter, P.M., Bergquist, S. and Sagarin, R. (2010) Reduction of Fecal Indicator Bacteria (FIB) in the Ballona Wetlands Saltwater Marsh (Los Angeles County, California, USA) with Implications for Restoration Actions. Water Research, 44, 4630-4642. http://dx.doi.org/10.1016/j.watres.2010.06.012

[14]   Elmund, G.K., Allen, M.J. and Rice, E.W. (1999) Comparison of Escherichia coli, Total Coliform, and Fecal Coliform Populations as Indicators of Wastewater Treatment Efficiency. Water Environment Research, 71, 332-339. http://dx.doi.org/10.2175/106143098X121752

[15]   Grabow, W.O.K., Hilner, C.A. and Coubrough, P. (1981) Evaluation of Standard and Modified M-FC, MacConkey, and Teepol Media for Membrane Filtration Counting of Fecal Coliforms in Water. Applied and environmental Microbiology, 42, 192-199.

[16]   Levine, M. (1918) Differentiation of B. Coli and B. Aerogenes on a Simplified Eosin-Methylene Blue Agar. The Journal of Infectious Diseases, 23, 43-47.

[17]   Grabow, W.O. and Du Preez, M. (1979) Comparison of m-Endo LES, MacConkey, and Teepol Media for Membrane Filtration Counting of Total Coliform Bacteria in Water. Applied and Environmental Microbiology, 38, 351-358.

[18]   Hogan, J.N., Daniels, M.E., Watson, F.G., Conrad, P.A., Oates, S.C., Miller, M.A. and Miller, W.A. (2012) Longitudinal Poisson regression to Evaluate the Epidemiology of Cryptosporidium, Giardia, and Fecal Indicator Bacteria in Coastal California Wetlands. Applied and Environmental Microbiology, 78, 3606-3613. http://dx.doi.org/10.1128/aem.00578-12

[19]   Martinez-Murcia, A.J., Anton, A.I. and Rodriguez-Valera, F. (1999) Patterns of Sequence Variation in Two Regions of the 16S rRNA Multigene Family of Escherichia coli. International Journal of Systematic Bacteriology, 49, 601-610. http://dx.doi.org/10.1099/00207713-49-2-601

[20]   Dombek, P.E., Johnson, L.K., Zimmerley, S.T. and Sadowsky, M.J. (2000) Use of Repetitive DNA Sequences and the PCR to Differentiate Escherichia coli Isolates from Human and Animal Sources. Applied and Environmental Microbiology, 66, 2572-2577. http://dx.doi.org/10.1128/AEM.66.6.2572-2577.2000

[21]   Johnson, T.J., Kariyawasam, S., Wannemuehler, Y., Mangiamele, P., Johnson, S.J., Doetkott, C. and Nolan, L.K. (2007) The Genome Sequence of Avian Pathogenic Escherichia coli Strain O1: K1: H7 Shares Strong Similarities with Human Extra-Intestinal Pathogenic E. coli Genomes. Journal of Bacteriology, 189, 3228-3236. http://dx.doi.org/10.1128/JB.01726-06

[22]   Harwood, V.J., Butler, J., Parrish, D. and Wagner, V. (1999) Isolation of Fecal Coliform Bacteria from the Diamondback Terrapin (Malaclemys terrapin centrata). Applied and Environmental Microbiology, 65, 865-867.

[23]   Gray, M.J., Rajeev, S., Miller, D.L., Schmutzer, A.C., Burton, E.C., Rogers, E.D. and Hickling, G.J. (2007) Preliminary Evidence that American Bullfrogs (Rana catesbeiana) Are Suitable Hosts for Escherichia coli O157: H7. Applied and Environmental Microbiology, 73, 4066-4068. http://dx.doi.org/10.1128/AEM.02905-06

[24]   Dipineto, L., Gargiulo, A., Russo, T.P., De Luca Bossa, L.M., Borrelli, L., d’ Ovidio, D. and Fioretti, A. (2010) Survey of Escherichia coli O157 in Captive Frogs. Journal of Wildlife Diseases, 46, 944-946. http://dx.doi.org/10.7589/0090-3558-46.3.944

 
 
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