ABB  Vol.6 No.8 , August 2015
Inter-Specific Biochemical Diversity between Echis pyramidum and Eryx colubrinus Inhabiting El-Faiyum, Egypt
Abstract: Discontinuous polyacrylamide gel electrophoreses for lactate dehydrogenase (Ldh) and alfa-esterase (α-Est) isoenzymes were conducted for biochemical differentiation between Echis pyramidum and Eryx colubrinus inhabiting El-Faiyum, Egypt. Total lipids and total protein of liver and muscle tissues in both species were also analyzed. Three Ldh isoforms were recorded in both species and the activity and relative front (RF) of Ldh-1 seemed to be higher in E. pyramidum than in E. colubrinus. This high activity could be supported by the significant increase in the total lipids and total protein in liver and muscle tissues of this species. It is thus possibly reasonable to suppose that E. pyramidum is more active, energetic and adaptable in its habitat than E. colubrinus. α-Est showed five isozymic forms fractions in E. pyramidum, while it showed only four isoforms in E. colubrinus. α-Est-1 was the first clear, dense and thick isoform in E. pyramidum, but it was completely absent in E. colubrinus. High activity of the esterase isoform; α-Est-1 only in the heart tissue of E. pyramidum may reflect the high ability of E. pyramidum to be more resistant to the accumulated toxic residues in its body tissues than E. colubrinus.
Cite this paper: A. M. Kadry, M. , Mohallal, E. , M. Sleem, D. and A. S. Marie, M. (2015) Inter-Specific Biochemical Diversity between Echis pyramidum and Eryx colubrinus Inhabiting El-Faiyum, Egypt. Advances in Bioscience and Biotechnology, 6, 495-500. doi: 10.4236/abb.2015.68051.

[1]   Vidal, N. and Hedges, S.B. (2009) The Molecular Evolutionary Tree of Lizards, Snakes, and Amphisbaenians. Comptes Rendus Biologies, 332, 129-139.

[2]   Goodman, S.M. and Hobbs, J.J. (1994) The Distribution an Ethnozoology of Reptiles of the Northern Portion of the Egyptian Eastern Desert. Journal of Ethnobiology, 14, 75-100.

[3]   Sayed, N.H.M. (2012) Genetic Diversity among Eight Egyptian Snakes (Squamata-Serpentes: Colubridae) Using RAPD-PCR. Life Science Journal, 9, 423-430.

[4]   Zug, E.R., Vitt, L.J. and Caldwell, J.P. (2001) Herpetology: An Introductory Biology of Amphibians and Reptiles. Academic Press, New York.

[5]   Sayed, N.H.M. (2011) Genetic Diversity among Five Egyptian Non-Poisonous Snakes Using Protein and Isoenzymes Electrophoresis. Life Science Journal, 8, 1034-1042.

[6]   Dowling, H.E., Hass, C.A., Hedges, S.B. and Highton, R. (1996) Snake Relationships Revealed by Slow-Evolving Total Protein: A Preliminary Survey. Journal of Zoology, 240, 1-28.

[7]   Lawson, R., Slowinski, J.B., Crother, B.I. and Burbrink, F.T. (2005) Phylogeny of the Colubroidea (Serpentes): New Evidence from Mitochondrial and Nuclear genes. Molecular Phylogenetics and Evolution, 37, 581-601.

[8]   Burbrink, F.T. and Pyron, R.A. (2008) The Taming of the Skew: Estimating Proper Confidence Intervals for Divergence Dates. Systematic Biology, 57, 317-328.

[9]   Wiens, J.J., Kuczynski, C.A., Smith, S.A., Mulcahy, D.E., Sites Jr., J.W., Townsend, T.M. and Reeder, T.W. (2008) Branch Lengths, Support, and Congruence: Testing the Phylogenomic Approach with 20 Nuclear Loci in Snakes. Systematic Biology, 57, 420-431.

[10]   Kelly, C.M.R., Barker, N.P., Villet, M.H. and Broadley, D.E. (2009) Phylogeny, Biogeography and Classification of the Snake Superfamily Elapoidea: A Rapid Radiation in the Late Eocene. Cladistics, 25, 38-63.

[11]   Vidal, N., Rage, J.C., Couloux, A. and Hedges, S.B. (2009) Snakes (Serpentes). In: Hedges, S.B. and Kumar, S., Eds., The Time Tree of Life, Oxford University Press, New York, 390-397.

[12]   Zaher, H., Grazziotin, F.E., Cadle, J.E., Murphy, R.W., Moura-Leite, J.C. and Bonatto, S.L. (2009) Molecular Phylogeny of Advanced Snakes (Serpentes, Caenophidia) with an Emphasis on South American Xenodontines: A Revised Classification and Descriptions of New Taxa. Papéis Avulsos de Zoologia, 49, 115-153.

[13]   Pyron, R.A., Burbrink, F.T., Colli, E.R., de Oca, A.N.M., Vitt, L.J., Kuczynski, C.A. and Wiens, J.J. (2011) The Phylogeny of Advanced Snakes (Colubroidea), with Discovery of a New Subfamily and Comparison of Support Methods for Likelihood Trees. Molecular Phylogenetics and Evolution, 58, 329-342.

[14]   Mallow, D., Ludwig, D. and Nilson, E. (2003) True Vipers: Natural History and Toxinology of Old World Vipers. Krieger Publishing Company, Malabar, 359 p.

[15]   McDiarmid, R.W., Campbell, J.A. and Touré, T. (1999) Snake Species of the World: A Taxonomic and Geographic Reference, Volume 1. Herpetologists’ League, Washington, District of Columbia, 511 p.

[16]   Mehrtens, J.M. (1987) Living Snakes of the World in Color. Sterling Publishers, New York, 480 p.

[17]   Zuckerkandl, E. and Pauling, L. (1965) Evolutionary Divergence and Convergence in Total Protein. In: Bryson, V. and Vogel, H.J., Eds., Evolving Genes and Total Protein, Academic Press, New York, 97-166.

[18]   Lewontin, R.C. (1974) The Genetic Basis of Evolutionary Change. Columbia University Press, New York.

[19]   Kimura, M. (1983) The Neutral Theory of Molecular Evolution. Cambridge University Press, Cambridge.

[20]   Al-Harbi, M.S. and Amer, S.A.M. (2012) Comparison of Energy-Related Isoenzymes between Production and Racing Arabian Camels. Advances in Bioscience and Biotechnology, 3, 1124-1128.

[21]   Shahjahan, R.M., Karim, A., Begum, R.A., Alam, M.S. and Begum, A. (2008) Tissue Specific Esterase Isozyme Banding Pattern in Nile Tilapia (Oreochromis niloticus). Universal Journal of Zoology (Rajshahi University), 27, 1-5.

[22]   El Din, M.B. (2006) A Guide to Reptiles and Amphibians of Egypt. American University in Cairo Press, Cairo, 359 p.

[23]   Maurer, R. (1968) Disk Electrophorese. W. de Gruyter and Co., Berlin, 222 p.

[24]   Shaw, C.R. and Prasad, R. (1970) Starch Gel Electrophoresis of Enzymes: A Compilation of Recipes. Biochemistry and Genetics, 4, 297-329.

[25]   Mulvey, M. and Vrijenhoek, R.C. (1981) Genetic Variation among Laboratory Strains of the Planorbid Snail Biomphalaria glabrata. Biochemical Genetics, 19, 1169-1182.

[26]   Jonathan, F.W. and Wendel, N.F. (1990) Visualization and Interpretation of Plant Isozymes. In: Soltis, D.E. and Soltis, P.S., Eds., Isozymes in Plant Biology, Champan and Hall, London, 5-45.

[27]   Scandaliojs, G. (1964) Tissue-Specific Isozyme Variations in Maize. Journal of Heredity, 55, 281-285.

[28]   Shaklee, J.B., Allendorf, F.W., Morizot, D.C. and Whitt, G.S. (1990) Gene Nomenclature for Protein Coding Loci in Fish. Transactions of the American Fisheries Society, 119, 2-15.<0002:GNFPLI>2.3.CO;2

[29]   Zöllner, N. and Kirsch, K. (1962) Colorimetric Method for Determination of Total Lipids. Journal of Experimental Medicine, 135, 545-550.

[30]   Gornall, A.G., Bardawill, C.J. and David, M.M. (1949) Determination of Serum Proteins by Means of the Biuret Reaction. Journal of Biological Chemistry, 177, 751-766.

[31]   Kadry, M.A.M. and Mohamad, H.R.H. (2014) Genetic and Metabolic Variability between Two Subspecies of Chamaeleo chamaeleon (Reptilia: Chamaeleonidae) in Egypt. Advances in Bioscience and Biotechnology, 5, 699-703.