JBiSE  Vol.8 No.7 , July 2015
Colorimetric Detection of Lead Ion Based on Gold Nanoparticles and Lead-Stabilized G-Quartet Formation
Abstract: In this report, we present a method for the detection of Pb2+ based on the different adsorption capacity on the surface of gold nanoparticles (AuNPs) between ssDNA (single-stranded DNA) and G-quartet. In the absence of Pb2+, the DNA oligonucleotides probe, which is guanine-rich ssDNA, can be adsorbed on the surface of AuNPs protecting them from aggregation. After adding Pb2+, the DNA oligonucleotides probe can specifically form compact G-quartet, which can induce the aggregation of unmodified AuNPs, especially after adding NaCl aqueous solution. Consequently, the color turns from red to blue. Pb2+ can be detected by colorimetric response of AuNPs; its detection limit can reach 5 μM only observed by naked eyes. Most metal ions have no interferences, and the interference of Cu2+ can be effectively eliminated by adding cysteine. It provides a simple and effective colorimetric sensor for on-site and real time detection of Pb2+.
Cite this paper: Chen, P. , Zhang, R. , Jiang, Q. , Xiong, X. and Deng, S. (2015) Colorimetric Detection of Lead Ion Based on Gold Nanoparticles and Lead-Stabilized G-Quartet Formation. Journal of Biomedical Science and Engineering, 8, 451-457. doi: 10.4236/jbise.2015.87042.

[1]   Goyer, R.A. (1990) Lead Toxicity: From Overt to Subclinical to Subtle Health Effects. Environmental Health Perspectives, 86, 177-181.

[2]   Bermejo-Barrera, P., Martinez Alfonso, N., Daz Lopez, C. and Bermejo Barrera, A. (2003) Use of Amberlite XAD-2 Loaded with 1-(2-Pyridylazo)-2-naphthol as a Preconcentration System for River Water Prior to Determination of Cu2+, Cd2+ and Pb2+ by Flame Atomic Absorption Spectroscopy. Microchimica Acta, 142, 101-108.

[3]   Kim, H.N., Ren, W.X., Kim, J.S. and Yoon, J. (2012) Fluorescent and Colorimetric Sensors for Detection of Lead, Cadmium, and Mercury Ions. Chemical Society Reviews, 41, 3210-3244.

[4]   Lin, Y.W., Huang, C.C. and Chang, H.T. (2011) Gold Nanoparticle Probes for the Detection of Mercury, Lead and Copper Ions. The Analyst, 136, 863-871.

[5]   Liu, J. and Lu, Y. (2003) A Colorimetric Lead Biosensor Using DNAzyme-Directed Assembly of Gold Nanoparticles. Journal of the American Chemical Society, 125, 6642-6643.

[6]   Mazumdar, D., Liu, J.W., Lu, G., Zhou, J.Z. and Lu, Y. (2010) Easy-to-Use Dipstick Tests for Detection of Lead in Paints Using Non-Cross-Linked Gold Nanoparticle-DNAzyme Conju-gates. Chemical Communications, 46, 1416-1418.

[7]   Wang, Z.D., Lee, J.H. and Lu, Y. (2008) Label-Free Colorimetric Detection of Lead Ions with a Nanomolar Detection Limit and Tunable Dynamic Range by Using Gold Nanoparticles and DNAzyme. Advanced Materials, 20, 3263-3267.

[8]   Liu, J. and Lu, Y. (2004) Colorimetric Biosensors Based on DNAzyme-Assembled Gold Nanoparticles. Journal of Fluorescence, 14, 343-354.

[9]   Hung, Y.L., Hsiung, T.-M., Chen, Y.-Y., Huang, Y.-F. and Huang, C.-C. (2010) Colorimetric Detection of Heavy Metal Ions Using Label-Free Gold Nanoparticles and Alkanethiols. The Journal of Physical Chemistry C, 114, 16329-16334.

[10]   Ding, N., Cao, Q., Zhao, H., Yang, Y.M., Zeng, L.X., He, Y.J., et al. (2010) Colorimetric Assay for Determination of Lead (II) Based on Its Incorporation into Gold Nanoparticles during Their Synthesis. Sensors, 10, 11144-11155.

[11]   Guo, Y., Wang, Z., Qu, W.S., Shao, H.W. and Jiang, X.Y. (2011) Colori-metric Detection of Mercury, Lead and Copper Ions Simultaneously Using Protein-Functionalized Gold Nanoparticles. Bio-sensors & Bioelectronics, 26, 4064- 4069.

[12]   Guan, J., Jiang, L., Zhao, L.L., Li, J. and Yang, W.S. (2008) pH-Dependent Response of Citrate Capped Au Nanoparticle to Pb2+ Ion. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 325, 194-197.

[13]   Yoosaf, K., Ipe, B.I., Suresh, C.H. and Thomas, K.G. (2007) In Situ Synthesis of Metal Nanoparticles and Selective Naked-Eye Detection of Lead Ions from Aqueous Media. The Journal of Physical Chemistry C, 111, 12839-12847.

[14]   Li, Y., Si, Y., Wang, X.Q., Ding, B., Sun, G., Zheng, G., et al. (2013) Colorimetric Sensor Strips for Lead (II) Assay Utilizing Nanogold Probes Immobilized Polyamide-6/Nitrocellulose Nano-Fibers/Nets. Biosensors & Bioelectronics, 48, 244-250.

[15]   Saha, K., Agasti, S.S., Kim, C., Li, X.N. and Rotello, V.M. (2012) Gold Nanoparticles in Chemical and Biological Sensing. Chemical Reviews, 112, 2739-2779.

[16]   Sigel, H. (1993) Interactions of Metal Ions with Nucleotides and Nucleic Acids and Their Constituents. Chemical Society Reviews, 22, 255-267.

[17]   Smirnov, I. and Shafer, R.H. (2000) Lead is Unusually Effective in Sequence-Specific Folding of DNA. Journal of Molecular Biology, 296, 1-5.

[18]   Ma, F., Sun, B., Qi, H.L., Zhang, H.G., Gao, Q. and Zhang, C.X. (2011) A Signal-On Electrogenerated Chemiluminescent Biosensor for Lead Ion Based on DNAzyme. Analytica Chimica Acta, 683, 234-241.

[19]   Wen, Y., Peng, C., Li, D., Zhuo, L., He, S.J., Wang, L.H., et al. (2011) Metal Ion-Modulated Graphene-DNAzyme Interactions: Design of a Nanoprobe for Fluorescent Detection of Lead(II) Ions with High Sensitivity, Selectivity and Tunable Dynamic Range. Chemical Communications, 47, 6278-6280.

[20]   Li, F., Yang, L.M., Chen, M.Q., Li, P. and Tang, B. (2013) A Selective Amperometric Sensing Platform for Lead Based on Target-Induced Strand Release. Analyst, 138, 461-466.

[21]   Liu, C.W., Huang, C.C. and Chang, H.T. (2009) Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions. Analytical Chemistry, 81, 2383-2387.

[22]   Zhan, S.S., Wu, Y.G., Liu, L., Xing, H.B., He, L., Zhan, X.J., et al. (2013) A Simple Fluorescent Assay for Lead(II) Detection Based on Lead(II)-Stabilized G-Quadruplex Formation. RSC Advances, 3, 16962-16966.

[23]   Li, T., Dong, S. and Wang, E. (2010) A Lead(II)-Driven DNA Molecular Device for Turn-On Fluorescence Detection of Lead(II) Ion with High Selectivity and Sensitivity. Journal of the American Chemical Society, 132, 13156-13157.

[24]   Xu, H., Liu, B.X. and Chen, Y. (2012) A Colorimetric Method for the Determination of Lead(II) Ions Using Gold Nanoparticles and a Guanine-Rich Oligonucleotide. Microchimica Acta, 177, 89-94.

[25]   Li, H. and Rothberg, L. (2004) Colorimetric Detection of DNA Sequences Based on Electrostatic Interactions with Unmodified Gold Nanoparticles. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 101, 14036-14039.

[26]   Li, B., Du, Y. and Dong, S. (2009) DNA Based Gold Nanoparticles Colorimetric Sensors for Sensitive and Selective Detection of Ag(I) Ions. Analytica Chimica Acta, 644, 78-82.

[27]   Wang, L., Liu, X.F., Hu, X.F., Song, S.P. and Fan, C.H. (2006) Unmodified Gold Nanoparticles as a Colorimetric Probe for Potassium DNA Aptamers. Chemical Communications, 36, 3780-3782.

[28]   Yang, W., Gooding, J.J., He, Z., Li, Q. and Chen, G. (2007) Fast Colorimetric Detection of Copper Ions Using L-Cysteine Functionalized Gold Nanoparticles. Nanoscience and Nanotechnology, 7, 712-716.

[29]   Lin, Z., Li, X. and Kraatz, H.B. (2011) Impedimetric Immobilized DNA-Based Sensor for Simultaneous Detection of Pb2+, Ag+, and Hg2+. Analytical Chemistry, 83, 6896-6901.