OJF  Vol.5 No.5 , July 2015
Cold-Resistant Breeding of Oenothera speciosa Using Silico Ion Implantation
Abstract: Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M1, M2 and M3 generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the LJ°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.
Cite this paper: Ma, Y. , Lin, Y. , Sun, L. , Song, L. , Cui, R. and Qie, Y. (2015) Cold-Resistant Breeding of Oenothera speciosa Using Silico Ion Implantation. Open Journal of Forestry, 5, 546-554. doi: 10.4236/ojf.2015.55047.

[1]   Atkinson, C. J., Brennan, R. M., & Jones, H. G. (2013). Declining Chilling and Its Impact on Temperate Perennial Crops. Environmental and Experimental Botany, 91, 48-62.

[2]   Chen, A. K., Han, R. H., Li, D. Y., Lin, L. L., Luo, H. X., & Tang, S. J. (2010). A Comparison of Two Methods for Electrical Conductivity about Plant Leaves. Journal of Guangdong Education Institute, 30, 88-91.

[3]   Duan, R. X., & Jiang, H. F. (2002). Agrometeorology. China Meteorological Press, Beijing.

[4]   Gao, F. Y., Zhang, J. L., Liu, Z. P., & Guo, F. F. (2010). Effects of Continuous Low-Temperature Stress on Electrical Conductivity and MDA Content of Garden Tree. Shandong Agricultural Sciences, 2, 47-49.

[5]   Jian, L. C., & Wang, H. (2009). Plant Cell Biology Under Stress. Science Press, Beijing.

[6]   Kalberer, S. R., Leyva-Estrada, N., Krebs, S. L., & Arora, R. (2007). Frost Dehardening and Rehardening of Floral Buds of Deciduous Azaleas Are Influenced by Genotypic Biogeography. Environmental and Experimental Botany, 59, 264-275.

[7]   Kim, D. C., & Anderson, N. O. (2006). Comparative Analysis of Laboratory Freezing Methods to Establish Cold Tolerance of Detached Rhizomes and Intact Crowns in Garden Chrysanthemums (Dendranthema × grandiflora Tzvelv.). Scientia Horticulturae, 109, 345-352.

[8]   Liu, Z. S., & Luo, H. R. (2010). Modern Plant Breeding. Science Press, Beijing.

[9]   Luo, L., Feng, D. Q., Liu, J., Wang, Y. S., & Zhao, J. H. (2011). The Preliminary Study on Cold Resistance of Variant Apricots Treated by Nitrogen Iron Implantation. Journal of Shandong Agricultural University (Natural Science), 42, 465-470.

[10]   Ma, Y., Lin, Y., Sun, L. P., Liang, F., & Dong, A. X. (2012). Research on Cold Resistance Breeding for Oenothera speciosa in Beijing. Pratacultural Science, 29, 1569-1573.

[11]   Mahadtanapuk, S., Teraarusiri, W., Phanchaisri, B., Yu, L. D., & Anuntalabhochai, S. (2013). Breeding for Blast-Disease-Resistant and High-Yield Thai Jasmine Rice (Oryza sativa L. cv. KDML 105) Mutants Using Low-Energy Ion Beams. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 307, 229-234.

[12]   Wan, H. (2011). Study on the Physiological and Biochemical Characters of Cashen Cold Resistance. Master’s Thesis, Southwest Forestry University, Kunming.

[13]   Wang, B. S. (2010). Plant Biology under Stress. Higher Education Press, Beijing.

[14]   Wang, L. J. (2010). Study on Cold Resistance of four Sedum PLANT. Master’s Thesis, Nanjing Forestry University, Nanjing.

[15]   Wang, X. Y., Zhang, Y. L., Zhang, H. M., & Yu, N. (2007). Research Advance of Silicon Biochemical Functions on Crop. Soil and Fertilizer Sciences in China, 6, 6-9.

[16]   Ye, J., Chen, D., & Shen, Y. (2012). Analysis of the Low Temperature Tolerance of Three Ornamental Plants of Michelia. Anhui Agriculture Scientific Bulletion, 18, 51-55.

[17]   Zheng, B. S. (2006). Research Techniques in Comtemporary Plant Physiology and Biochemistry. China Meteorological Press, Beijing.