Irenus A. Tazisong^1*, Zachary N. Senwo¹, Zhongqi He²

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¹ Department of Biological & Environmental Sciences, Alabama A&M University, Normal, AL, USA.
² USDA-ARS Southern Regional Research Center, New Orleans, LA, USA.
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Abstract: Phosphatases are diverse groups of enzymes that deserve special attention because of their significant roles in organic phosphorus (OP) mineralization to inorganic available forms (Pi). This work 1) compared the catalytic potentials of commercially acid phosphatase from wheat germ, sweet potato, and potato, and alkaline phosphatase from E. coli; 2) demonstrated that the rate of hydrolysis, catalytic efficiency, thermal stability, and optimal pH of these enzymes depended on enzyme sources and the stereochemical or stereoisomeric structures of the substrates; 3) revealed that both acid and alkaline phosphatases exhibited broad range of substrate hydrolysis with high affinity for p-nitrophenyl phosphate bis (cyclohexylammonium) than the widely used p-nitrophenyl phosphate disodium hexahydrate for phosphatase assay. Sweet potato had relatively higher reaction kinetics (Vmax, Km, Kcat, Kcat/Km) values with most substrates tested. The order of catalytic activity was in the order: sweet potato > wheat germ > potato, while the order of substrate hydrolyzed was: PNPBC > PNP > PNP2A2E > DG6P2Na > DG6PNa > Bis-PNP > phytate. The optimum pH for the acid phosphatase was observed to be 5.0. Generally, the activity of alkaline phosphatase was similar to that of acid phosphatase with optimal pH between 10 and 13, depending on the substrates. Knowledge derived from this work would be helpful in enzyme catalysis in soils and water environments.

Keywords: Enzymes, Phosphatases, Organic Phosphorus Mineralization, Wheat Germ, Sweet Potato, E. coli

Cite this paper: Tazisong, I. , Senwo, Z. and He, Z. (2015) Phosphatase Hydrolysis of Organic Phosphorus Compounds. Advances in Enzyme Research, 3, 39-51. doi: 10.4236/aer.2015.32005.

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