Paralytic shellfish poisoning (PSP)
toxins are potent neurotoxins mainly produced by dinoflagellates and being concentrated
in bivalves through food web transfer. Increasing number of findings of toxin-producing
bacteria in the cells of dinoflagellate such as Alexandriumtamarense supports the
hypothesis of the bacterial origin of PSP toxins. Evidence that there are specific
symbiosis bacterial taxa associated with the phytoplankton indicates the presence
of specific selective mechanisms between them, and implies that the symbiosis bacteria
have some vital function to the benefit of the dinoflagellates. Studies on the role
of toxin-producing symbiosis bacteria in the marine ecosystem are considered to
be becoming more important. Although toxigenic bacteria could be isolated from toxic
dinoflagellates, it was not clearly proven whether the isolated bacterial strains
based on culture-dependent manner and the corresponding intracellular bacteria were
the same because of microbial unculturability. This paper aims to demonstrate the
biodiversity of the symbiotic bacteria associated with toxic dinoflagellate A. tamarense
using the culture-indepen- dent high-throughput pyrosequencing method, as well as
the phylogenetic analysis based on 16S rDNA sequences of the symbiotic cultivable
bacteria strains isolated from toxic Alexander tamarense.
Cite this paper
Zhang, X. , Tian, X. , Ma, L. , Feng, B. , Liu, Q. , Yuan, L. , Fan, C. , Huang, H. , Huang, H. , Yang, Q. (2015) Biodiversity of the Symbiotic Bacteria Associated with Toxic Marine Dinoflagellate Alexandrium tamarense. Journal of Biosciences and Medicines
, 23-28. doi: 10.4236/jbm.2015.36004
 Thottumkara, A.P., Parsons, W.H. and Du Bois, J. (2014) Saxitoxin. Angewandte Chemie International Edition, 53, 5760-5784. http://dx.doi.org/10.1002/anie.201308235
 Zhang F., Xu, X., Li, T. and Liu, Z. (2013) Shellfish Toxins Targeting Voltage-Gated Sodium Channels. Mar Drugs, 11, 4698-4723. http://dx.doi.org/10.3390/md11124698
 Orr, R.J., Stüken, A., Murray, S.A. and Jakobsen, K.S. (2013) Evolution and Distribution of Saxitoxin Biosynthesis in Dinoflagellates. Marine Drugs, 11, 2814-2828. http://dx.doi.org/10.3390/md11082814
 Etheridge, S.M. (2010) Paralytic Shellfish Poisoning: Seafood Safety and Human Health Perspectives. Toxicon, 56, 108-122. http://dx.doi.org/10.1016/j.toxicon.2009.12.013
 Cembella, A.D. (1998) Ecophysiology and Metabolism of Paralytic Shellfish Toxins in Marine Microalgae. In: Anderson, O.M., Cembella, A.D. and Hallegraeff, G.M., Eds., Physiological Ecology of Harmful Algal Blooms, NATO ASI Series Vol. G41, Springer-Verlag, Berlin, Heidelberg, 381-403.
 Kodama, M. (2010) Paralytic Shellfish Poisoning Toxins: Biochemistry and Origin. Terrapub, Orono.
 Gallacher, S. and Smith, E.A. (1999) Bacteria and Paralytic Shellfish Toxins. Protist, 150, 245-255.
 Gallacher, S., Flynn, K.J., Franco, J.M., Brueggemann, E.E. and Hines, H.B. (1997) Evidence for Production of Paralytic Shellfish Toxins by Bacteria Associated with Alexandrium spp. (Dinophyta) in Culture. Applied and Environmental Microbiology, 63, 239-245.
 Green, D.H., Llewellyn, L.E., Negri, A.P., Blackburn, S.I. and Bolch, C.J. (2004) Phylogenetic and Functional Diversity of the Cultivable Bacterial Community Associated with the Paralytic Shellfish Poisoning Dinoflagellate Gymnodinium catenatum. FEMS Microbiology Ecology, 47, 345-357. http://dx.doi.org/10.1016/S0168-6496(03)00298-8
 Dantzer, W.R. and Levin, R.E. (1997) Bacterial Influence on the Production of Paralytic Shellfish Toxins by Dinoflagellated Algae. Journal of Applied Microbiology, 83, 464-469. http://dx.doi.org/10.1046/j.1365-2672.1997.00246.x
 Kodama, M., Ogata, T., Sakamoto, S., Sato, S., Honda, T. and Miwatani, T. (1990) Production of Paralytic Shellfish Toxins by a Bacterium Moraxella sp. Isolated from Protogonyaulax tamarensis. Toxicon, 28, 707-714.
 Kodama, M., Sakamoto, S. and Koike, K. (1996) Symbiosis of Bacteria in Alexandrium tamarense. Harmful and Toxic Algal Bloom. Intergovernmental Oceanographic Commission of UNESCO, Paris, 351-354.
 McCann, J.C., Wickersham, T.A. and Loor, J.J. (2014) High-Throughput Methods Redefine the Rumen Microbiome and Its Relationship with Nutrition and Metabolism. Bioinformatics and Biology In-sights, 8, 109.
 Andersson, A. F., Lindberg, M., Jakobsson, H., B?ckhed, F., Nyrén, P. and Engstrand, L. (2008) Comparative Analysis of Human Gut Microbiota by Barcoded Pyrosequencing. PloS ONE, 3, e2836.