GSC  Vol.5 No.2 , May 2015
Effect of Nitrogen Source on Biomass and Lipid Production of a Marine Microalga, Nannochloropsis oceanica IMET1
ABSTRACT
The effects of the nitrogen sources sodium nitrate (NaNO3) and urea (CH4N2O) on growth, lipid production, and fatty acid composition of Nannochloropsis oceanica IMET1 were investigated. Nitrogen source affected cell density, dry cell weight, and lipid production. Cells grown in the nitrate medium increased dry cell weight and lipid weight in comparison with cells grown in the urea medium. The composition of fatty acids varied with nitrogen sources. IMET1cultured in the nitrate medium mainly contained C18:2 (14.9%) and C16:0 (6.3%) fatty acids, while IMET1 in the urea medium mainly contained C22:0 (33.1%), C18:3 (8.6%), and C16:0 (6.8%). This study demonstrates that nitrogen source can strongly influence lipid production and composition of N. oceanica IMET1.

Cite this paper
Chi, Y. , Chen, F. and Takiguchi, Y. (2015) Effect of Nitrogen Source on Biomass and Lipid Production of a Marine Microalga, Nannochloropsis oceanica IMET1. Green and Sustainable Chemistry, 5, 101-106. doi: 10.4236/gsc.2015.52013.
References
[1]   Dong, H.P., Williams, E., Wang, D.Z., Xie, Z.X., Hsia, R.C., Jenck, A., Halden, R., Li, J., Chen, F. and Place, A.R. (2013) Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery. Plant Physiology, 162, 1110-1126.
http://dx.doi.org/10.1104/pp.113.214320

[2]   Kilian, O., Benemann, C.S., Niyogi, K.K. and Vick, B. (2011) High-Efficiency Homologous Recombination in the Oil-Producing Alga Nannochloropsis sp. Proceedings of the National Academy of Sciences, 108, 21265-21269.
http://dx.doi.org/10.1073/pnas.1105861108

[3]   Sukenik, A., Carmeli, Y. and Berner, T. (1989) Regulation of Fatty Acid Composition by Irradiance Level in the Eustigmatophyte Nannochloropsis sp. Journal of Phycology, 25, 686-692.
http://dx.doi.org/10.1111/j.0022-3646.1989.00686.x

[4]   Sukenik, A., Zamora, O. and Carmeli, Y. (1993) Biochemical Quality of Marine Unicellular Algae with Emphasis on Lipid Composition. II. Nannochloropsis sp. Aquaculture, 117, 313-326.
http://dx.doi.org/10.1016/0044-8486(93)90328-V

[5]   Suen, Y., Hubbard, J.S., Holzer, G. and Tornabene, T.G. (1987) Total Lipid Production of the Green Alga Nannochloropsis sp. QII under Different Nitrogen Regimes. Journal of Phycology, 23, 289-296.
http://dx.doi.org/10.1111/j.1529-8817.1987.tb04137.x

[6]   Xiao, Y., Zhang, J., Cui, J., Feng, Y. and Cui, Q. (2013) Metabolic Profiles of Nannochloropsis oceanica IMET1 under Nitrogen-Deficiency Stress. Bioresource Technology, 130, 731-738.
http://dx.doi.org/10.1016/j.biortech.2012.11.116

[7]   Chi, Y. and Takiguchi, Y. (2015) Comparison of the Growth Performance of Nannnochloropsis oceanica IMET1 and Nannnochloropsis gaditana CCMP526 in Various Cultivation Conditions. Journal of Plant Science, 3, 9-13.

[8]   Campos, H., Boeing, J.W. and Barry N. (2014) Dungan, Tanner Schaub, Cultivating the Marine Microalga Nannochloropsis salina under Various Nitrogen Sources: Effect on Biovolume Yields, Lipid Content and Composition, and Invasive Organisms. Biomass and Bioenergy, 66, 301-307.
http://dx.doi.org/10.1016/j.biombioe.2014.04.005

[9]   Li, Y., Horsman, M., Wang, B., Wu, N. and Lan, C.Q. (2008) Effect of Nitrogen Sources on Cell Growth and Lipid Accumulation of Green Alga Neochloris oleoabundans. Applied Microbiology and Biotechnology, 81, 629-636.

[10]   Li, X., Hu, H.-Y., Gan, K. and Yang, J. (2010) Growth and Nutrient Removal Properties of a Freshwater Miroalga Scenedesmus sp. LX1 under Different Kinds of Nitrogen Sources. Ecological Engineering, 36, 379-381.
http://dx.doi.org/10.1016/j.ecoleng.2009.11.003

[11]   Goksan, T., Ak, I. and Kilic, C. (2011) Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration. Turkish Journal of Fisheries and Aquatic Sciences, 11, 377-383.
http://dx.doi.org/10.4194/1303-2712-v11_3_06

[12]   Rodolfi, L., Zittelli, G.C., Bassi, N., Padobani, G., Biondi, N., Bonini, G. and Tredici, M.R. (2009) Microalgae for Oil: Strain Selection, Induction of Lipid Synthesis and Outdoor Mass Cultivation in a Low-Cost Photobioreactor. Biotechnology and Bioengineering, 102, 100-112.
http://dx.doi.org/10.1002/bit.22033

[13]   Converti, A., Casazza, A,A., Ortiz, E.Y., Perego, P. and Del, B.M. (2009) Effect of Temperature and Nitrogen Concentration on the Growth and Lipid Content of Nannochloropsis oculata and Chlorella vulgaris for Biodiesel Production. Chemical Engineering and Processing, 48, 1146-1151.
http://dx.doi.org/10.1016/j.cep.2009.03.006

[14]   Renaud, S.M. and Parry, D.L. (1994) Microalgae for Use in Tropical Aquaculture. II. Effect of Salinity on Growth, Gross Chemical-Composition and Fatty-Acid Composition of 3 Species of Marine Microalgae. Journal of Applied Phycology, 6, 347-356.
http://dx.doi.org/10.1007/BF02181949

[15]   Blight, E.G. and Dyer, W.J. (1956) A rapid Method of Total Lipid Extraction of Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917.
http://dx.doi.org/10.1139/o59-099

 
 
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