JCDSA  Vol.5 No.2 , June 2015
Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC): Occlusive Effect and Penetration Enhancement Ability
Abstract: Objective: This work compares the occlusive effect and the penetration enhancement ability of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), through in vitro skin. Methods: SLN and NLC were prepared by high shear homogenization and characterized by size, polydispersity index, zeta potential, morphology and physical stability. Occlusive effect was assessed by an in vitro test and by measuring TEWL using pig skin. Skin treated with the lipid carriers was visualized by SEM. A penetration test through skin, followed by tape stripping, was carried out using Nile red as a marker. Results: SLN (200 ± 6 nm) and NLC (192 ± 11 nm) were obtained. An occlusion factor of 36% - 39% was observed for both systems, while a reduction in TEWL of 34.3% ± 14.8% and 26.2% ± 6.5% was seen after treatment with SLN and NLC, respectively. SEM images showed a film formed by the lipid carriers, responsible for the occlusion observed. No differences were found between the occlusive effect produced by SLN and NLC in both tests. NLC allowed the penetration of a greater amount of Nile red than SLN: 4.7 ± 1.3 μg and 1.7 ± 0.4 μg, respectively. Conclusion: Both carriers form a film on the skin, providing an occlusive effect with no differences between these two systems. The penetration of a marker (Nile red) into the stratum corneum was quite higher for NLC than for SLN, suggesting an influence of the composition of these particles on their penetration enhancing ability.
Cite this paper: López-García, R. and Ganem-Rondero, A. (2015) Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC): Occlusive Effect and Penetration Enhancement Ability. Journal of Cosmetics, Dermatological Sciences and Applications, 5, 62-72. doi: 10.4236/jcdsa.2015.52008.

[1]   Müller, R.H., Radteke, M. and Wissing, S.A. (2002) Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) in Cosmetic and Dermatological Preparations. Advanced Drug Delivery Reviews, 54, S131-S155.

[2]   Jores, K., Mehnert, W., Drechsler, M., Bunjes, H., Johann, C. and Mader, K. (2004) Investigations on the Structure of Solid Lipid Nanoparticles (SLN) and Oil-Loaded Solid Lipid Nanoparticles by Photon Correlation Spectroscopy, Field-Flow Fractionation and Transmission Electron Microscopy. Journal of Controlled Release, 95, 217-227.

[3]   Teeranachaideekul, V., Müller, R.H. and Junyaprasert, V.B. (2007) Encapsulation of Ascorbyl Palmitate in Nanostructured Lipid Carriers (NLC)—Effects of Formulation Parameters on Physicochemical Stability. International Journal of Pharmaceutics, 340, 198-206.

[4]   Contri, R.V., Fiel, L.A., Pohlman, A.R., Guterres, S.S. and Beck, R.C.R. (2011) Transport of Substances and Nanoparticles across the Skin and in Vitro Models to Evaluate Skin Permeation and/or Penetration. In: Beck, R., Guterres, S. and Pohlman, A., Eds., Nanocosmetics and Nanomedicines, New Approaches for Skin Care, Springer, Berlin, 3-35.

[5]   Puglia, C., Damiani, E., Offerta, A., Rizza, L., Tirendi, G.G., Tarico, M.S., Curreri, S., Bonina, F. and Perrotta, R.E. (2014) Evaluation of Nanostructured Lipid Carriers (NLC) and Nanoemulsions as Carriers for UV-Filters: Characterization, in Vitro Penetration and Photostability Studies. European Journal of Pharmaceutical Sciences, 51, 211-217.

[6]   Souto, E.B. and Müller, R.H. (2008) Cosmetic Features and Application of Lipid Nanoparticles (SLN, NLC). International Journal of Cosmetic Science, 30, 157-165.

[7]   Wissing, S., Lippacher, A. and Müller, R. (2001) Investigations on the Occlusive Properties of Solid Lipid Nanoparticles (SLN). Journal of Cosmetic Science, 52, 313-324.

[8]   Wissing, S. and Müller, R. (2003) The Influence of Solid Lipid Nanoparticles on Skin Hydration and Viscoelasticity— In Vivo Study. European Journal of Pharmaceutics and Biopharmaceutics, 56, 67-72.

[9]   Pardeike, J., Hommoss, A. and Müller, R. (2009) Lipid Nanoparticles (SLN, NLC) in Cosmetic and Pharmaceutical Dermal Products. International Journal of Pharmaceutics, 366, 170-184.

[10]   Müller, R., Petersen, R., Hommoss, A. and Pardeike, J. (2007) Nanostructured Lipid Carriers (NLC) in Cosmetic Dermal Products. Advanced Drug Delivery Reviews, 59, 522-530.

[11]   Iannuccelli, V., Coppi, G., Romagnolli, M., Sergi, S. and Leo, E. (2013) In Vivo Detection of Lipid-Based Nano- and Microparticles in the Outermost Human Stratum Corneum by EDX Analysis. International Journal of Pharmaceutics, 447, 204-212.

[12]   Schwarz, J., Weixelbaum, A., Pagitsch, E., Low, M., Resch, G. and Valenta, C. (2012) Nanocarriers for Dermal Drug Delivery: Influence of Preparation Method, Carrier Type and Rheological Properties. International Journal of Pharmaceutics, 437, 83-88.

[13]   Montenegro, L., Sinico, C., Castangia, I., Carbone, C. and Puglisi, G. (2012) Idebenone-Loaded Solid Lipid Nanoparticles for Drug Delivery to the Skin: In Vitro Evaluation. International Journal of Pharmaceutics, 434, 169-174.

[14]   Tiwari, R. and Pathak, K. (2011) Nanostructured Lipid Carrier Versus Solid Lipid Nanoparticles of Simvastatin: Comparative Analysis of Characteristics, Pharmacokinetics and Tissue Uptake. International Journal of Pharmaceutics, 415, 232-243.

[15]   Kovacevic, A., Savic, S., Vuleta, G., Müller, R. and Keck, C. (2011) Polyhydroxy Surfactants for the Formulation of Lipid Nanoparticles (SLN and NLC): Effects on Size, Physical Stability and Particle Matrix Structure. International Journal of Pharmaceutics, 406, 163-172.

[16]   Das, S., Kiong, W. and Tan, R. (2012) Are Nanostructured Lipid Carriers (NLCs) Better Than Solid Lipid Nanoparticles (SLNs): Development, Characterizations and Comparative Evaluations of Clotrimazole-Loaded SLNs and NLCs? European Journal of Pharmaceutical Sciences, 47, 139-151.

[17]   Bose, S. and Michniak-Kohn, B. (2013) Preparation and Characterization of Lipid Based Nanosystems for Topical Delivery of Quercetin. European Journal of Pharmaceutical Sciences, 48, 442-452.

[18]   Teeranachaideekul, V., Boonme, P., Souto, E.B., Müller, R.H. and Junyaprasert ,V.B. (2008) Influence of Oil Content on Physicochemical Properties and Skin Distribution of Nile Red-Loaded NLC. Journal of Controlled Release, 128, 134-141.

[19]   Levin, J. and Maibach, H. (2009) The Correlation between Transepidermal Water Loss and Percutaneous Absorption: An Overview. In: Barel, A., Paye, M. and Maibach, H. Eds, Handbook of Cosmetic Science and Technology, Informa Healthcare, NewYork, 165-171.

[20]   Mehnert, W. and Mader, K. (2001) Solid Lipid Nanoparticles: Production, Characterization and Applications. Advanced Drug Delivery Reviews, 47, 165-196.

[21]   Garzón, M., Vázquez, M., Villafuerte, L., García, B. and Hernández, A. (2009) Effect of Formulation Components on the Properties of Solid Lipid Nanoparticles. Revista Mexicana De Ciencias Farmacéuticas, 2, 26-40.

[22]   Rowe, R., Sheskey, P. and Owen, S. (2006) Handbook of Pharmaceutical Excipients. Pharmaceutical Press, London, 304-305, 535-538.

[23]   Wissing, S. and Müller, R. (2001) A Novel Sunscreen System Based on Tocopherol Acetate Incorporated into Solid Lipid Nanoparticles. International Journal of Cosmetic Science, 23, 233-243.

[24]   Wissing, S. and Müller, R. (2003) Cosmetic Applications for Solid Lipid Nanoparticles (SLN). International Journal of Pharmaceutics, 254, 65-68.

[25]   Jenning, V., Gysler, A., Schafer-Korting, M. and Gohla, S. (2000) Vitamin A Loaded Solid Lipid Nanoparticles for Topical Use: Occlusive Properties and Drug Targeting to the Upper Skin. European Journal of Pharmaceutics and Biopharmaceutics, 49, 211-218.

[26]   Jensen, L., Petersson, K. and Nielsen, H. (2011) In Vitro Penetration Properties of Solid Lipid Nanoparticles in Intact and Barrier-Impaired Skin. European Journal of Pharmaceutics and Biopharmaceutics, 79, 68-75.

[27]   James, K.C. (1986) Solubility and Related Properties. Marcel Dekker, New York.

[28]   Lombardi, S., Regehly, M., Sivaramakrishnan, R., Mehnert, W., Korting, H.C., Danker, K., Roder, B., Kramer, K.D. and Schafer-Korting, M. (2005) Lipid Nanoparticles for Skin Penetration Enhancement—Correlation to Drug Localization within the Particle Matrix as Determined by Fluorescence and Parelectric Spectroscopy. Journal of Controlled Release, 110, 151-163.