Back
 FNS  Vol.6 No.8 , May 2015
Continuous Subcutaneous Administration of Miso Extracts Attenuates Salt-Induced Hypertension in Dahl Salt-Sensitive Rats
Abstract: Objective: Long-term intake of Miso attenuates hypertension in Dahl salt-sensitive (Dahl S) rats through an increased urinary sodium excretion. We examined whether a bolus injection into the peritoneal cavity (i.p.) or a continuous subcutaneous infusion of a Miso extract attenuates hypertension in Dahl S rats. Materials and Methods: We investigated the effects of a bolus, i.p. injection of 50 mg Miso extract in 0.5 mL on hypertension in Dahl S rats, and examined whether a long-term subcutaneous infusion of the Miso extract (50 mg Miso/day), using an osmotic mini-pump working for 14 days, attenuates hypertension in Dahl S rats. Results: A bolus, i.p. injection of 50 mg Miso extract decreased SBP in 2 hrs. The reduction was significant at 4 hrs, and SBP returned to the baseline at 24 hrs. The SBP reduction at 4 hrs after the injection was increasingly greater each day during the 4 days. The SBP reduction by the Miso extract was dose-dependent and the antihy-pertensive activity occurs in a <5 kDa fraction of the extract. The subcutaneous infusion of 50 mg Miso extract/day for 14 days significantly attenuated hypertension in Dahl S rats. The SBP reduction was associated with significant decreases in the heart and kidney weights. Urinary protein excretion significantly decreased in the Miso group. The SBP reduction was not associated with increases in either urinary sodium excretion or fractional excretion of sodium. Conclusions: SBP reduction by very low-dose of the Miso extract may be mediated partly by mechanisms other than renal sodium handling.
Cite this paper: Shimizu, N. , Du, D. , Sakuyama, H. , Ito, Y. , Sonoda, M. , Kawakubo, K. and Uehara, Y. (2015) Continuous Subcutaneous Administration of Miso Extracts Attenuates Salt-Induced Hypertension in Dahl Salt-Sensitive Rats. Food and Nutrition Sciences, 6, 693-702. doi: 10.4236/fns.2015.68072.
References

[1]   Yoshinaga, M., Toda, N., Tamura, Y., Terakado, S., Ueno, M., Otsuka, K., Numabe, A., Kawabata, Y., Uehara, Y. (2012) Japanese Traditional Miso Soup Attenuates Salt-Induced Hypertension and Its Organ Damage in Dahl Salt-Sensitive Rats. Nutrition, 28, 924-931.
http://dx.doi.org/10.1016/j.nut.2011.09.010

[2]   Du, D.D., Yoshinaga, M., Sonoda, M., Kawakubo, K. and Uehara, Y. (2013) Blood Pressure Reduction by Japanese Traditional Miso Is Associated with Increased Diuresis and Natriuresis through Dopamine System in Dahl Salt-Sensitive Rat. Clinical and Experimental Hypertension, 36, 359-366.

[3]   Watanabe, H., Kashimoto, N., Kajimura, J. and Kamiya, K. (2006) A Miso (Japanese Soybean Paste) Diet Conferred Greater Protection against Hypertension than a Na Chloride Diet in Dahl Salt-Sensitive Rats. Hypertension Research, 29, 731-738.
http://dx.doi.org/10.1291/hypres.29.731

[4]   Ebisawa, K., Miyamoto, Y., Du, D.D., Yoshinga, M. and Uehara, Y. (2012) The Effects of Long-Term Miso Soup on Na Absorption in the Intestine of Wistar Rats. The 35th Scientific Meeting of Japanese Society of Hypertension, Nagoya, 20-22 September 2012.

[5]   Friedman, M. and Freed, S.C. (1949) Microphonic Manometer for Indirect Determination of Systolic Blood Pressure in the Rat. Proceedings of the Society for Experimental Biology and Medicine, 70, 670-672.
http://dx.doi.org/10.3181/00379727-70-17028

[6]   Hirawa, N., Uehara, Y., Kawabata, Y., Ohshima, N., Ono, H., Nagata, T., Gomi, T., Ikeda, T., Goto, A., Yagi, S. and Omata, M. (1995) Subpressor Dose of Angiotensin II Increases Susceptibility to the Haemodynamic Injury of Blood Pressure in Dahl Salt-Sensitive Rats. Journal of Hypertension, 13, 81-90.
http://dx.doi.org/10.1097/00004872-199501000-00013

[7]   Uehara, Y., Numabe, A., Hirawa, N., Kawabata, Y., Iwai, J., Ono, H., Matsuoka, H., Takabatake, Y., Yagi, S. and Sugimoto, T. (1991) Antihypertensive Effects of Cicletanine and Renal Protection in Dahl Salt-Sensitive Rats. Journal of Hypertension, 9, 719-728.
http://dx.doi.org/10.1097/00004872-199108000-00005

[8]   Moriya, C., Shida, Y., Yamane, Y., Miyamoto, Y., Kimura, M., Huse, N., Ebisawa, K., Kameda, Y., Nishi, Y., Du, D.D., Yoshinaga, M., Murota, I., Sato, N. and Uehara, Y. (2013) Subcutaneous Administration of Sodium Alginate Oligosaccharides Prevents Salt-Induced Hypertension in Dahl Salt-Sensitive Rats. Clinical and Experimental Hypertension, 35, 607-613.
http://dx.doi.org/10.3109/10641963.2013.776568

[9]   Martinez-Villaluenga, C., Torino, M.I., Martín, V., Arroyo, R., Garcia-Mora, P., Estrella Pedrola, I., Vidal-Valverde, C., Rodriguez, J.M. and Frias, J. (2012) Multifunctional Properties of Soy Milk Fermented by Enterococcus faecium Strains Isolated from Raw Soy Milk. Journal of Agricultural and Food Chemistry, 60, 10235-10244.
http://dx.doi.org/10.1021/jf302751m

[10]   Tomatsu, M., Shimakage, A., Shinbo, M., Yamada, S. and Takahashi, S. (2013) Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Derived from Soya Milk. Food Chemistry, 136, 612-616.
http://dx.doi.org/10.1016/j.foodchem.2012.08.080

[11]   Farzamirad, V. and Aluko, R.E. (2008) Angiotensin-Converting Enzyme Inhibition and Free-Radical Scavenging Properties of Cationic Peptides Derived from Soybean Protein Hydrolysates. International Journal of Food Sciences and Nutrition, 59, 428-437.
http://dx.doi.org/10.1080/09637480701592897

[12]   Appel, L.J., Moore, T.J., Obarzanek, E., William, M., Volomer, W.M., Svetkey, L.P., Sacks, F.M., Bray, G.A., Vogt, T.M., Cutler, J.A., Windhauser, M.M., Lin, P.-H. and Karanja, N., The DASH Collaborative Research Group (1997) A Clinical Trial of the Effects of Dietary Patterns on Blood Pressure. New England Journal of Medicine, 336, 1117-1124.
http://dx.doi.org/10.1056/NEJM199704173361601

[13]   Sacks, F.M., Svetkey, L.P., Vollmer, W.M., Appel, L.J., Bray, G.A., Harsha, D., Obarzanek, E., Conlin, P.R., Miller 3rd, E.R., Simons-Morton, D.G., Karanja, N. and Lin, P.H. (2001) DASH-Na Collaborative Research Group: Effects on Blood Pressure of Reduced Dietary Na and the Dietary Approaches to Stop Hypertension (DASH) Diet. DASH-Na Collaborative Research Group. New England Journal of Medicine, 344, 3-10.
http://dx.doi.org/10.1056/NEJM200101043440101

[14]   Watanabe, H. (2013) Beneficial Biological Effects of Miso with Reference to Radiation Injury, Cancer and Hypertension. Journal of Toxicologic Pathology, 26, 91-103.
http://dx.doi.org/10.1293/tox.26.91

[15]   Nevala, R., Vaskonen, T., Vehniainen, J., Korpela, R. and Vapaatalo, H. (2000) Soy Based Diet Attenuates the Development of Hypertension When Compared to Casein Based Diet in Spontaneously Hypertensive Rat. Life Science, 66, 115-124.
http://dx.doi.org/10.1016/S0024-3205(99)00569-X

[16]   He, J., Gu, D., Wu, X., Chen, J., Duan, X., Chen, J. and Whelton, P.K. (2005) Effect of Soybean Protein on Blood Pressure: A Randomized, Controlled Trial. Annals of Internal Medicine, 143, 1-9.
http://dx.doi.org/10.7326/0003-4819-143-1-200507050-00004

[17]   Velasques, M.T. and Bhathena, S.J. (2007) Role of Dietary Soy Protein in Obesity. International Journal of Medical Sciences, 4, 72-82.
http://dx.doi.org/10.7150/ijms.4.72

[18]   Known, D.Y., Hong, S.M., Ahn, I.I.S., Kim, M.J., Yang, H.J. and Park, S. (2011) Isoflavonoids and Peptides from Meju, Long-Term Fermented Soybeans, Increase Insulin Sensitivity and Exert Insulinotropic Effects in Vitro. Nutrition, 27, 244-252.
http://dx.doi.org/10.1016/j.nut.2010.02.004

 
 
Top