Back
 AJAC  Vol.6 No.6 , May 2015
Investigation of Iron Complex Formation of Anti-Hypertensive Drug: Methyldopa
Abstract: The drug administered for any disease may play an unwanted function in biological system. They may have multiple counter effects, one of which is their interaction to bioactive metals. Iron is most common bio essential metal and is reported to interact with antihypertensive drug methyldopa. In the present study, above said complex is analyzed by UV-Visible spectrophotometry. Formation constant of the complex is calculated by using mole ratio method and single point statistical method which is in the range of 1010, values are also calculated which are independent of pH like formation constant. Absorbance maxima were found to be dependent on pH. At lower pH complex shows two broad bands centered at 430 nm and 730 nm. With the rise in pH later peak shifts toward lower wavelength, so 615 nm is selected for further studies. Molar extinction coefficient of the complex is explored by serial dilution method. At all wavelengths it increases with increase in pH. Mole ratio and slope ratio methods are used for exploring stoichiometry. Metal to ligand combining ratio in the complex is 1:2 at pH 4.0 and pH 4.5 while 1:3 at pH 5.0 and pH 5.5.
Cite this paper: Fiaz, T. , Fatima, N. , Zaidi, S. , Abbas, T. and Kazimi, M. (2015) Investigation of Iron Complex Formation of Anti-Hypertensive Drug: Methyldopa. American Journal of Analytical Chemistry, 6, 551-558. doi: 10.4236/ajac.2015.66053.
References

[1]   La Piana Simonsen, L. (1989) Top 200 Drugs of 1988. Pharmacy Times, 40.

[2]   Osman, M.A., Patel, R.B., Schuna, A., Sundstrom, W.R. and Welling, P.G. (1983) Reduction in Oral Penicillamine Absorption by Food, Antacid, and Ferrous Sulfate. Clinical Pharmacology and Therapeutics, 33, 465-470.
http://dx.doi.org/10.1038/clpt.1983.63

[3]   Campbell, N.R.C. and Hasinoff, B. (1989) Ferrous Sulfate Reduces Levodopa Bioavailability: Chelation as a Possible Mechanism. Clinical Pharmacology and Therapeutics, 45, 220-225.
http://dx.doi.org/10.1038/clpt.1989.21

[4]   Campbell, R.R.A., Hasinoff, B., Chemenko, G., Barrowman, J. and Campbell, N.R.C. (1990) The Effect of Ferrous Sulfate and pH on 1-Dopa Absorption. Canadian Journal of Physiology and Pharmacology, 68, 603-607.
http://dx.doi.org/10.1139/y90-087

[5]   Campbell, N.R.C., Ranfine, D., Goodridge, A.E., Hasinoff, B.B. and Kara, M. (1990) Sinemet-Ferrous Sulphate Interaction in Patients with Parkinson’s Disease. British Journal of Clinical Pharmacology, 30, 599-605.
http://dx.doi.org/10.1111/j.1365-2125.1990.tb03819.x

[6]   Polk, R.E., Healy, D.P., Sahai, J., Drwal, L. and Racht, E. (1989) Effect of Ferrous Sulfate and Multivitamins with Zinc on Absorption of Ciprofloxacin in Normal Volunteers. Antimicrobial Agents and Chemotherapy, 33, 1841-1844.
http://dx.doi.org/10.1128/AAC.33.11.1841

[7]   Campbell, N.R.C., Paddock, V. and Sundaram, R. (1988) Alteration of Methyldopa Absorption, Metabolism and Blood Pressure Control Caused by Ferrous Sulfate and Ferrous Gluconate. Clinical Pharmacology and Therapeutics, 43, 381-386.
http://dx.doi.org/10.1038/clpt.1988.47

[8]   Campbell, N.R.C., Hasinoff, B. and Campbell, R.R.A. (1990) Ferrous Sulfate Reduces Methyldopa Absorption: Methyldopairon Complex Formation as a Likely Mechanism. Clinical and Investigative Medicine, 13, 329-332.

[9]   Hoffman, B.B., Lefkowitz, R.J., Gilman, A.G., Hardman, J.G., Limbird, L.E., Molinoff, P.B. and Rudon, R.W. (1996) The Pharmacological Basis of Therapeutics. 9th Edition, MacGraw-Hill, New York.

[10]   The United States Pharmacopoeial Convention, Rockville, M.D. (2000) The United States Pharmacopoeia. 24th Edition, The National Formulary, 19.

[11]   Amin, D. (1986) Titrimetric Determination of Catecholamines and Related Compounds via Bromine Oxidation and Substitution. The Analyst, 111, 255-257.
http://dx.doi.org/10.1039/an9861100255

[12]   Walash, M.I., Abou-Ouf, A. and Salem, F.B. (1985) Spectrophotometric Determination of Methyldopa in Pharmaceutical Formulations. Journal of the Association of Official Analytical Chemists, 68, 91.

[13]   Mohamed, W.I. and Salem, F.B. (1984) Spectrophotometrio and Titrimetric Determination of Certain Adrenergic Drugs. Analytical Letters, 17, 191-203.
http://dx.doi.org/10.1080/00032718408065278

[14]   Salem, F.B. (1987) Spectrophotometric and Titrimetric Determination of Catecholamines. Talanta, 34, 810-812.
http://dx.doi.org/10.1016/0039-9140(87)80101-7

[15]   Salem, F.B. (1993) Titrimetric and Spectrophotometric Determination of Catecholamines. Analytical Letters, 26, 1959-1966.
http://dx.doi.org/10.1080/00032719308017443

[16]   Salem, F.B. (1993) Spectrophotometric and Fluorimetric Determination of Catecholamines. Analytical Letters, 26, 281-294.
http://dx.doi.org/10.1080/00032719308017385

[17]   Martinez-Lozano, C., Pérez-Ruiz, T., Tomas, V. and Val, O. (1991) Determination of Epinephrine, Norepinephrine, Dopamine and L-Dopa in Pharmaceuticals by a Photokinetic Method. The Analyst, 116, 857-859.
http://dx.doi.org/10.1039/an9911600857

[18]   Garrido, M.E., Lima, J.L.F.C. and Delerue-Mattos, C. (1997) Flow Injection Amperometric Determination of L-Dopa, Epinephrine or Dopamine in Pharmaceutical Preparations. Journal of Pharmaceutical and Biomedical Analysis, 15, 845-849.

[19]   Sharma, C., Mohanty, S., Kumar, S. and Rao, N.J. (1996) Gas Chromatographic Analysis of Chlorophenolic, Resin and Fatty Acids in Chlorination and Caustic Extraction Stage Effluent from Kahi-Grass. The Analyst, 121, 1963-1967.
http://dx.doi.org/10.1039/an9962101963

[20]   Lee, H.B., Hong-You, R.L. and Fowlie, P.J. (1989) Chemical Derivatization Analysis of Phenols. Part VI. Determination of Chlorinated Phenolics in Pulp and Paper Effluents. Journal of the Association of Official Analytical Chemists, 72, 979-984.

[21]   Tsuchiya, H., Sato, M., Kato, H., Okubo, T., Juneja, L.R. and Kim, M. (1997) Simultaneous Determination of Catechins in Human Saliva by High-Performance Liquid Chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 703, 253-258.
http://dx.doi.org/10.1016/S0378-4347(97)00412-X

[22]   Parsons, L.R., Kerr, T.M. and Weiss, F. (1998) Simple Microbore High-Performance Liquid Chromatographic Method for the Determination of Dopamine and Cocaine from a Single in Vivo Brain Microdialysis Sample. Journal of Chromatography B: Biomedical Sciences and Applications, 709, 35-45.
http://dx.doi.org/10.1016/S0378-4347(98)00024-3

[23]   Nozaki, O., Iwaeda, T. and Kato, Y. (1996) Amines for Detection of Dopamine by Generation of Hydrogen Peroxide and Peroxyoxalate Chemiluminescence. Journal of Bioluminescence and Chemiluminescence, 11, 309-313.
http://dx.doi.org/10.1002/(SICI)1099-1271(199611)11:6<309::AID-BIO424>3.0.CO;2-6

[24]   Kozminski, K.D., Gutman, D.A., Davila, V., Sulzer, D. and Ewing, A.G. (1998) Voltammetric and Pharmacological Characterization of Dopamine Release from Single Exocytotic Events at Rat Pheochromocytoma (PC12) Cells. Analytical Chemistry, 70, 3123-3130.
http://dx.doi.org/10.1021/ac980129f

[25]   Fiaz, T., Fatima, N. and Zaidi, S.Z.A. (2013) Complexation of Iron by Dopamine Analogs: A Spectrophotometric and Potentiometric Study. Pakistan Journal of Chemistry, 3, 75-80.
http://dx.doi.org/10.15228/2013.v03.i02.p06

[26]   Fatima, N., Zaidi, S.Z.A., Nisar, S. and Qadri, M. (2013) pH Effect on Stoichiometry and Stability of Ferrous Complexes of (-)-3-(3,4-Dihydroxyphenyl)-L-alanine. Pakistan Journal of Chemistry, 3, 23-28.
http://dx.doi.org/10.15228/2013.v03.i01.p04

[27]   Zaidi, S.Z.A. and Fatima, N. (2014) A Comparative Study for Chelation of Iron(II) and Iron(III) with Levodopa—An Antiparkinsonian Drug Molecule. European Chemical Bulletin, 3, 648-653.

[28]   Lykos, P. (1992) The Beer-Lambert Law Revisited: A Development without Calculus. Journal of Chemical Education, 69, 730-732.
http://dx.doi.org/10.1021/ed069p730

[29]   Skoog, D.A., Holler, F.J. and Nieman, T.A. (1998) Principles of Instrumental Analysis. Saunders College Publishing, Philadelphia.

[30]   Sawyer, D.T., Heineman, W.R. and Beebe, J.M. (1984) Chemistry Experimental for Instrumental Methods. John Wiley and Sons, Inc., Hoboken.

 
 
Top