JBiSE  Vol.4 No.6 , June 2011
Bioreactor for the reconstitution of a decellularized vascular matrix of biological origin
Abstract: Acellular matrices derived from animal and human cadaveric donor vessels or other tubular matrices are appropriate candidates for the creation of tissue- en-gineered, small-diameter, muscular arteries. Engi-neering principles have been used to design a bio-reactor and the necessary auxiliary systems for the reconstitution of a previously decellularized vascular matrix. The bioreactor enables the attachment of cells to the luminal and/or exterior surfaces of the matrix. For the recellularization procedure, the matrix is situated within a sealed compartment in order to maintain a sterile environment. The matrix is rotated continuously to assure a spatially uniform re-constitution. The auxiliary systems that serve the bioreactor are: (a) an oxygenator, (b) peristaltic pumps, one for conveying the internal cell medium and the other for conveying the external cell medium, (c) motor and gearing to create steady and controlled rotation, (d) reservoirs for the containment of the two media, and (e) tubing to convey the respective fluids and to interconnect the bioreactor culture chamber to the various auxiliary components. A recellularized matrix produced by the bioreactor demonstrated its capabilities to reconstitute a previously decellularized scaffold.
Cite this paper: nullGeeslin, M. , Caron, G. , Kren, S. , Sparrow, E. , Hultman, D. and Taylor, D. (2011) Bioreactor for the reconstitution of a decellularized vascular matrix of biological origin. Journal of Biomedical Science and Engineering, 4, 435-442. doi: 10.4236/jbise.2011.46055.

[1]   Isenberg, B.C., Williams, C. and Tranquillo, R.T. (2006) Small-diameter artificial arteries engineered in vitro. Circulation Research, 98, 25-35.

[2]   Teebken, O.E. and Haverich, A. (1999) Tissue engineering of small diameter vascular grafts. European Journal of Vascular and Endovascular Surgery, 23, 475-485. doi:10.1053/ejvs.2002.1654

[3]   Schmidt, C.E. and Baier, J.M. (2000) Acellular vascular tissues: Natural biomaterials for tissue repair and tissue engineering. Biomaterials, 21, 2215-2231. doi:10.1016/S0142-9612(00)00148-4

[4]   Bader, A., Schilling, T., Teebken, O.E., Brandes, G., Herden, T., Steinhoff, G. and Haverich, A. (1998) Tissue engineering of heart valves-human endothelial cell seeding of detergent acellularized porcine valves. European Journal of Cardiothorac Surgery, 14, 279-284. doi:10.1016/S1010-7940(98)00171-7

[5]   Bishopric, N.H., Dousman, L. and Yao, Y-MM. (1999) Matrix substrate for a viable body tissue-derived prosthesis and method for making the same. St. Jude Medical Inc., St Paul.

[6]   Livesy, S.A., del Campo, A.A., Nag, A., Nichols, K.B. and Coleman, C. (1994) Method for processing and preserving collagen-based tissues for transplantation. US patent 5 336 616, LifeCell Corp., Branchburg.

[7]   Sung, H.W., Hsu, C.S., Chen, H.C., Hsu, H.L., Chang, Y., Lu, J.H. and Yang, P.C. (1997) Fixation of various porcine arteries with an epoxy compound. Artificial Organs, 21, 50-58. doi:10.1111/j.1525-1594.1997.tb00699.x

[8]   Badylak, S.F., Record, R., Lindberg, K., Hodde, J. and Park, K. (1998) Small intestinal submucosa: A substrate for in vitro cell growth. Journal of Biomaterial Science and Polymers, 9, 863-878. doi:10.1163/156856298X00208

[9]   Robotin-Johnson, M.C., Swanson, P.E., Johnson, D.C., Schuessler, R.B. and Cox, J.L. (1998) An experimental model of small intestinal submucosa as a growing vascular graft. Journal of Thoracic Cardiovascular Surgery, 116, 805-811. doi:10.1016/S0022-5223(98)00436-X

[10]   Voytik-Harbin, S.L., Brightman, A.O., Kraine, M.R., Waisner, B. and Badylak, S.F. (1997) Identification of extractable growth factors from small intestinal submucosa. Journal of Cellular Biochemistry, 67, 478-491. doi:10.1002/(SICI)1097-4644(19971215)67:4<478::AID-JCB6>3.0.CO;2-P

[11]   Sandusky, G.E. Jr, Badylak, S.F., Morff, R.J., Johnson, W.D. and Lantz, G. (1992) Histologic findings after in vivo placement of small intestine submucosal vascular grafts and saphenous vein grafts in the carotid artery in dogs. American Journal of Pathology, 140, 317-324.

[12]   Huynh, T., Abraham, G., Murray, J., Brockbank, K., Hagen, P.O. and Sullivan, S. (1999) Remodeling of an acellular collagen graft into a physiologically responsive neovessel. Nature Biotechnology, 17, 1083-1086. doi:10.1038/15062