Modifications of L-Glutamine and L-Leucine Transport in Proliferating Lymphocytes

Jose Luis  Gil; Francisco  Sanchez; Macarena  Perán

doi:10.4236/jbm.2015.33016

Francisco Sanchez¹, Jose Luis Gil¹, Macarena Perán²

Abstract: Lymphocytes respond to mitogens that stimulate proliferation by increasing theirs metabolic activity. In this study we investigate L-Glutamine and L-Leucine uptake as markers of cell response to Concavalina A (ConcaA) stimulation, using a high-resolution flow technique. We found that lymphocytes induced to blast transformation enhanced rate and efficiency of amino acid uptake during cell proliferation. Considering that increases in transport is the first quantifiable response of cells during malignant transformation, amino acid uptake could also be useful as an early marker of malignancy.

Keywords: Cell Membrane Transport, Lymphocytes, L-Glutamine, L-Leucine, Malignancy Biomarkers

Cite this paper: Sanchez, F. , Gil, J. and Perán, M. (2015) Modifications of L-Glutamine and L-Leucine Transport in Proliferating Lymphocytes. Journal of Biosciences and Medicines, 3, 104-109. doi: 10.4236/jbm.2015.33016.

References

[1] Nagata, C., Wada, K., Tuji, M., Hayashi, M., Takeda, N. and Yasuda, K. (2013) Plasma Aminoacid Profiles Are Associated with Biomarkers of Breast Cancer Risk in Premenopausal Japanese Women. Cancer Causes & Control, 25, 143- 149. http://dx.doi.org/10.1007/s10552-013-0316-8

[2] Carr, E.L., Kelman, A., Wu, G.S., Ravindra, G., Emilee, S., Aghvanyan, A., et al. (2010) Glutamine Uptake and Metabolism Are Coordinately Regulated by ERK/MAPK during T Lymphocyte. Journal of Immunology, 185, 1037-1044. http://dx.doi.org/10.4049/jimmunol.0903586

[3] Shennan, D.B., Thomson, J., Gow, I.F., Travers, M.T. and Barber, M.C. (2004) L-Leucine Transport in Human Breast Cancer Cells (MCF-7 and MDA-MB-231): Kinetics, Regulation by Estrogen and Molecular Identity of the Transporter. Biochimica et Biophysica Acta, 1664, 206-216. http://dx.doi.org/10.1016/j.bbamem.2004.05.008

[4] Koch, B., Schröder, M.T., Schafer, G. and Schauder, P. (1990) Comparation between Transport and Degradation of Leucine and Glutamine by Peripheral Human Lymphocytes Exposed to Concanavalin A. Journal of Cellular Physiology, 143, 94-99. http://dx.doi.org/10.1002/jcp.1041430112

[5] Perán, S. and McGee, M.P. (1986) Unidirectional Flux of Phenylalanine into Vero Cells. Measurements Using Paired Tracers in Perfused Cultures. Biochimica et Biophysica Acta, 856, 231-236. http://dx.doi.org/10.1016/0005-2736(86)90032-5

[6] Smolková, K. and Jezek, P. (2012) The Role of Mitochondrial NADPH-Dependent Isocitrate Dehydrogenase in Cancer Cells. International Journal of Cell Biology, 2012, Article ID: 273947. http://dx.doi.org/10.1155/2012/273947

[7] Kenneth, A. and Craig, B. (2004) Regulation of T Lymphocyte Metabolism. Thompson. The Journal of Immunology, 172, 4661-4665. http://dx.doi.org/10.4049/jimmunol.172.8.4661

[8] Yaqoob, P. and Calder, P.C. (1997) Glutamine Requirement of Proliferating T Lymphocytes. Nutrition, 13, 646-651. http://dx.doi.org/10.1016/S0899-9007(97)83008-0

[9] Marko, A.J., Miller, R.A., Kelman, A. and Frauwirth, K.A. (2010) Induction of Glucose Metabolism in Stimulated T Lymphocytes Is Regulated by Mitogen-Activated Protein Kinase Signaling. PLoS ONE, 5, e15425. http://dx.doi.org/10.1371/journal.pone.0015425

[10] Qu, W., Oya, S., Lieberman, B.P., Ploessl, K., Wang, L., Wise, D.R., Divgi, C.R., et al. (2012) Preparation and Characterization of L-[5-11C]-Glutamine for Metabolic Imaging of Tumors. Journal of Nuclear Medicine, 53, 98-105. http://dx.doi.org/10.2967/jnumed.111.093831

[11] Segel, G.B., Simon, W. and Lichtman, M.A. (1985) A Multicomponent Analysis of Amino Acid Transport Systems in Human Lymphocytes. Kinetic Parameters of the A- and L-Systems and Path-ways of Uptake of Naturally Occurring Amino Acids in Blood Lymphocytes. Journal of Cellular Physiology, 116, 372-378. http://dx.doi.org/10.1002/jcp.1041160315