In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus
Author(s)
Zuraida Ab Rahman1,
Erny Sabrina Mohd Noor2,
Mohd Shukri Mat Ali2,
Razali Mirad2,
Ayu Nazreena Othman1
Affiliation(s)
1 Biotechnology Research Centre, MARDI Headquarters, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia. 2 Strategic Resource Centre, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia.
1 Biotechnology Research Centre, MARDI Headquarters, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia. 2 Strategic Resource Centre, MARDI HQ, Persiaran MARDI-UPM, Serdang, Malaysia.
ABSTRACT
The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.
The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.
Cite this paper
Rahman, Z. , Mohd Noor, E. , Mat Ali, M. , Mirad, R. and Othman, A. (2015) In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus. American Journal of Plant Sciences, 6, 1091-1097. doi: 10.4236/ajps.2015.68113.
Rahman, Z. , Mohd Noor, E. , Mat Ali, M. , Mirad, R. and Othman, A. (2015) In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus. American Journal of Plant Sciences, 6, 1091-1097. doi: 10.4236/ajps.2015.68113.
References
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http://dx.doi.org/10.1007/s00436-010-1996-6 [3] Lukhoba, C.W., Simmonds, M.S.J. and Paton, A.J. (2006) Plectranthus: A Review of Ethnobotanical Uses. Journal of Ethnopharmacology, 103, 1-24.
http://dx.doi.org/10.1016/j.jep.2005.09.011 [4] Chang, J.M., Cheng, C.M., Hung, L.M., Chung, Y.S. and Wu, R.Y. (2010) Potential Use of Plectranthus amboinicus in the Treatment of Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine, 7, 115-120.
http://dx.doi.org/10.1093/ecam/nem168 [5] Oliveira, R.A.G., Torres, A.R., Diniz, M.F.F.M. and Araújo, E.C. (2005) Estudo sobre o uso de plantas medicinais em crianças hospitalizadas da cidade de João Pessoa: Riscos e benefícios. Brazilian Journal of Pharmacognsosy, 15, 373-380. [6] Pereira, C.O., Lima, E.O., Oliveira, R.A.G., Toledo, M.S., Azevedo, A.K.A., Guerra, M.F. and Pereira, R.C. (2005) Ethnobotanic Study of Medicinal Plants Used in Dermatological Disorders in João Pessoa-Paraíba, Brazil. Revista Brasileira de Plantas Médicinais, 3, 9-17. [7] Diniz, M.F.F.M. (1997) Memento fitoterápico: As plantas como alternativa terapêutica: Conhecimentos populares e científicos. Ed Universitária/UFPB, João Pessoa. [8] Lorenzi, H. and Matos, F.J.A. (2002) Plantas medicinais no Brasil: Nativas e exóticas. Nova. [9] Sudharson, S., Anbazhagan, M., Balachandran, B. and Arumugam, K. (2014) Effect of BAP on in Vitro Propagation of Hybanthus enneaspermus (L.) Muell, an Important Medicinal Plant. International Journal of Current Microbiology and applied Science, 3, 397-402. [10] Dharaneeswara, D.R.R., Dasari, S. and Muralidhra, R.D. (2014) Effects of 6-Benzyl Amino Purine (6-Bap) on in Vitro Shoot Multiplication of Grand Naine (Musa Sp.). International Journal of Advanced Biotechnology and Research, 5, 36-42. [11] Yohannes, S. and Firew, M. (2014) In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia edulis Hemsl.). Agriculture, Forestry and Fisheries, 3, 452-458. [12] Skoog, F. and Miller, C.O. (1957) Chemical Regulation of Growth and Organ Formation in Plant Tissues Cultured in Vitro. Symposia of the Society for Experimental Biology, 11, 118-130. [13] Aggarwal, D. and Barna, K.S. (2004) Tissue Culture Propagation of Elite Plant of Aloe vera Linn. Journal of Plant Biochemistry and Biotechnology, 13, 77-79.
http://dx.doi.org/10.1007/BF03263197 [14] Liao, Z.H., Chen, M., Tan, F., Sun, X.F. and Tang, K.X. (2004) Microprogagation of Endangered Chinese Aloe. Plant Cell, Tissue and Organ Culture, 76, 83-86.
http://dx.doi.org/10.1023/A:1025868515705 [15] Debiasi, C., Silva, C.G. and Pescador, R. (2007) Micropropagation of Aloe vera L. Revista Brasileira de Plantas Medicinais, 9, 36-43. [16] Farsi, M. and Zolali, J. (2011) Principles of Plant Biotechnology. Ferdowsi University of Mashhad Press, Mashhad, 553. [17] Brand, M.H. and Lineberger, R.D. (1988) In Vitro Adventitious Shoot Formation on Mature Phase Leaves and Petioles of Liquidamber styraciflua L. Plant Science (Limerick), 57, 173-179.
http://dx.doi.org/10.1016/0168-9452(88)90084-2 [18] Rao, P.S., Bapat, V.A., Mhatre, M. and Patel, G.K. (1989) Application of Plant Cell, Tissue and Organ Culture in Mulberry Improvement Programme. In: Sengupta, K. and Dandin, S.B., Eds., Genetic Resources of Mulberry and Utilization, Central Sericulture Research and Training Institute (CSR&TI), Mysore, 125-130. [19] Mariya, P., Ely, Z. and Antonina, V. (2011) Effect of Silver Nitrate on in Vitro Root Formation of Gentiana lutea. ACRomanian Biotechnological Letters, 16, 53-58. [20] Arun, K., Pingal, K. and Shukla, L.N. (2012) In Vitro Rooting in the Tissue Culture Raised Plantlets of Malbhog Cultivar of Banana. Indian Journal of Innovations and Developments, 1, 665-668. [21] Ravanfar, S.A., Aziz, M.A., Kadir, M.A., Rashid, A.A. and Sirchi, M.H.T. (2009) Plant Regeneration of Brassica oleracea subsp. italica (Broccoli) CV Green Marvel as Affected by Plant Growth Regulators. African Journal of Biotechnology, 8, 2523-2528.
[1] Hertzberg, H. and Buaer, C. (2000) Anthemintika-Resistenzen bei Magandarmstrongylidan von Schafen und Ziegen: Aktuelles uber Verbreitung, Epidemiologie, Vorbeuge-massnahmen und Alternativen zum anthelminthika Einsatz. Berliner und Münchener Tierärztliche Wochenschrift, 113, 122-128. [2] Senthilkumar, A. and Venkatesalu, V. (2010) Chemical Composition and Larvicidal Activity of the Essential Oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: A Malarial Vector Mosquito. Parasitology Research, 107, 1275-1278.
http://dx.doi.org/10.1007/s00436-010-1996-6 [3] Lukhoba, C.W., Simmonds, M.S.J. and Paton, A.J. (2006) Plectranthus: A Review of Ethnobotanical Uses. Journal of Ethnopharmacology, 103, 1-24.
http://dx.doi.org/10.1016/j.jep.2005.09.011 [4] Chang, J.M., Cheng, C.M., Hung, L.M., Chung, Y.S. and Wu, R.Y. (2010) Potential Use of Plectranthus amboinicus in the Treatment of Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine, 7, 115-120.
http://dx.doi.org/10.1093/ecam/nem168 [5] Oliveira, R.A.G., Torres, A.R., Diniz, M.F.F.M. and Araújo, E.C. (2005) Estudo sobre o uso de plantas medicinais em crianças hospitalizadas da cidade de João Pessoa: Riscos e benefícios. Brazilian Journal of Pharmacognsosy, 15, 373-380. [6] Pereira, C.O., Lima, E.O., Oliveira, R.A.G., Toledo, M.S., Azevedo, A.K.A., Guerra, M.F. and Pereira, R.C. (2005) Ethnobotanic Study of Medicinal Plants Used in Dermatological Disorders in João Pessoa-Paraíba, Brazil. Revista Brasileira de Plantas Médicinais, 3, 9-17. [7] Diniz, M.F.F.M. (1997) Memento fitoterápico: As plantas como alternativa terapêutica: Conhecimentos populares e científicos. Ed Universitária/UFPB, João Pessoa. [8] Lorenzi, H. and Matos, F.J.A. (2002) Plantas medicinais no Brasil: Nativas e exóticas. Nova. [9] Sudharson, S., Anbazhagan, M., Balachandran, B. and Arumugam, K. (2014) Effect of BAP on in Vitro Propagation of Hybanthus enneaspermus (L.) Muell, an Important Medicinal Plant. International Journal of Current Microbiology and applied Science, 3, 397-402. [10] Dharaneeswara, D.R.R., Dasari, S. and Muralidhra, R.D. (2014) Effects of 6-Benzyl Amino Purine (6-Bap) on in Vitro Shoot Multiplication of Grand Naine (Musa Sp.). International Journal of Advanced Biotechnology and Research, 5, 36-42. [11] Yohannes, S. and Firew, M. (2014) In Vitro Germination and Direct Shoot Induction of Yeheb (Cordeauxia edulis Hemsl.). Agriculture, Forestry and Fisheries, 3, 452-458. [12] Skoog, F. and Miller, C.O. (1957) Chemical Regulation of Growth and Organ Formation in Plant Tissues Cultured in Vitro. Symposia of the Society for Experimental Biology, 11, 118-130. [13] Aggarwal, D. and Barna, K.S. (2004) Tissue Culture Propagation of Elite Plant of Aloe vera Linn. Journal of Plant Biochemistry and Biotechnology, 13, 77-79.
http://dx.doi.org/10.1007/BF03263197 [14] Liao, Z.H., Chen, M., Tan, F., Sun, X.F. and Tang, K.X. (2004) Microprogagation of Endangered Chinese Aloe. Plant Cell, Tissue and Organ Culture, 76, 83-86.
http://dx.doi.org/10.1023/A:1025868515705 [15] Debiasi, C., Silva, C.G. and Pescador, R. (2007) Micropropagation of Aloe vera L. Revista Brasileira de Plantas Medicinais, 9, 36-43. [16] Farsi, M. and Zolali, J. (2011) Principles of Plant Biotechnology. Ferdowsi University of Mashhad Press, Mashhad, 553. [17] Brand, M.H. and Lineberger, R.D. (1988) In Vitro Adventitious Shoot Formation on Mature Phase Leaves and Petioles of Liquidamber styraciflua L. Plant Science (Limerick), 57, 173-179.
http://dx.doi.org/10.1016/0168-9452(88)90084-2 [18] Rao, P.S., Bapat, V.A., Mhatre, M. and Patel, G.K. (1989) Application of Plant Cell, Tissue and Organ Culture in Mulberry Improvement Programme. In: Sengupta, K. and Dandin, S.B., Eds., Genetic Resources of Mulberry and Utilization, Central Sericulture Research and Training Institute (CSR&TI), Mysore, 125-130. [19] Mariya, P., Ely, Z. and Antonina, V. (2011) Effect of Silver Nitrate on in Vitro Root Formation of Gentiana lutea. ACRomanian Biotechnological Letters, 16, 53-58. [20] Arun, K., Pingal, K. and Shukla, L.N. (2012) In Vitro Rooting in the Tissue Culture Raised Plantlets of Malbhog Cultivar of Banana. Indian Journal of Innovations and Developments, 1, 665-668. [21] Ravanfar, S.A., Aziz, M.A., Kadir, M.A., Rashid, A.A. and Sirchi, M.H.T. (2009) Plant Regeneration of Brassica oleracea subsp. italica (Broccoli) CV Green Marvel as Affected by Plant Growth Regulators. African Journal of Biotechnology, 8, 2523-2528.