Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer

Amitava  Chatterjee; Kirat Kumar  Ganguly; Sekhar  Pal; Shyamsundar  Mandal; Jaydip  Biswas

doi:10.4236/jct.2015.64040

Journals by Subject

Journals by Title

JCT Vol.6 No.4 , April 2015

Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer

Sekhar Pal¹, Kirat Kumar Ganguly¹, Shyamsundar Mandal², Jaydip Biswas³, Amitava Chatterjee¹^*

Abstract: Increased MMP-1 expression in various tumor cells is significantly correlated with cancer progression. Enhanced secreted and intracellular level of MMP-1 is found in breast cancer cell line MDA-MB-231 in presence of ECM glycoprotein fibronectin. To extrapolate this study into in vivo system, we observed the expression of MMP-1, fibronectin and α5β1 integrin, which is the receptor for fibronectin, in breast cancer tissue samples. Expression of active form of MMP-1 was increased in tumor samples compared with the non-tumor counterpart. In some samples pro-MMP-1 was decreased but active form was increased in tumor part. The difference was more prominent in advanced stage tumor. ELISA showed appreciable increase in expression of α5 and β1integrins in tumor tissue in comparison to the non tumor counterpart in case of advanced stage tumor. Though there is no appreciable difference in fibronectin concentration, enhanced α5, β1 integrin expression may mediate enhanced cell-ECM interaction to upregulate MMP-1 in tumor samples compared with the matched control.

Keywords: α5β1 Integrin, Signaling, ECM, Fibronectin, MMP-1

Cite this paper: Pal, S. , Ganguly, K. , Mandal, S. , Biswas, J. and Chatterjee, A. (2015) Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer. Journal of Cancer Therapy, 6, 375-382. doi: 10.4236/jct.2015.64040.

References

[1] Richie, R.C. and Swanson, J.O. (2003) Breast Cancer: A Review of the Literature. Journal of Insurance Medicine, 35, 85-101.

[2] Anderson, B.O. and Jakesz, R. (2008) Breast Cancer Issues in Developing Countries: An Overview of the Breast Health Global Initiative. World Journal of Surgery, 32, 2578-2585.
http://dx.doi.org/10.1007/s00268-007-9454-z

[3] Khokhar, A. (2012) Breast Cancer in India: Where Do We Stand and Where Do We go? Asian Pacific Journal of Cancer Prevention, 13, 4861-4866.
http://dx.doi.org/10.7314/APJCP.2012.13.10.4861

[4] Agarwal, G., Pradeep, P.V., Aggarwal, V., Yip, C.H. and Cheung, P.S. (2007) Spectrum of Breast Cancer in Asian Women. World Journal of Surgery, 31, 1031-1040.
http://dx.doi.org/10.1007/s00268-005-0585-9

[5] Kohrmann, A., Kammerer, U., Kapp, M., Dietl, J. and Anacker, J. (2009) Expression of Matrix Metalloproteinases (MMPs) in Primary Human Breast Cancer and Breast Cancer Cell Lines: New Findings and Review of The Literature. BMC Cancer, 9, 188.
http://dx.doi.org/10.1186/1471-2407-9-188

[6] Sil, H., Moulik, S., Singha, S., Mandal, S.S., Biswas, J. and Chatterjee, A. (2015) Activation of Matrix Metalloproteinase 2(MMP-2) in Breast Cancer Progression. Journal of Tumor, 3, 292-304.

[7] Nanda, D.P., Sil, H., Moulik, S., Biswas, J, Mandal, S.S. and Chatterjee, A. (2013) Matrix Metalloproteinase-9 as a Potential Tumor Marker in Breast Cancer. Journal of Environmental Pathology, Toxicology and Oncology, 32, 115-129.

[8] Rutter, J.L., Mitchell, T.I., Buttice, G., Meyers J, Gusella JF, Ozelius, L.J. and Brinckerhoff, C.E. (1998) A Single Nucleotide Polymorphism in the Matrix Metalloproteinase-1 Promoter Creates an Ets Binding Site and Augments Transcription. Cancer Research, 58, 5321-5325.

[9] Iyer, S., Visse, R., Nagase, H. and Acharya, K.R. (2006) Crystal Structure of an Active Form of Human MMP-1. Journal of Molecular Biology, 362, 78-88.
http://dx.doi.org/10.1016/j.jmb.2006.06.079

[10] Murray, G.I., Duncan, M.E., O’Neil, P., McKay, J.A., Melvin, W.T. and Fothergill J.E. (1998) Matrix Metalloproteinase-1 Is Associated with Poor Prognosis in Oesophageal Cancer. The Journal of Pathology, 185, 256-261.
http://dx.doi.org/10.1002/(SICI)1096-9896(199807)185:3<256::AID-PATH115>3.0.CO;2-A

[11] Murray, G.I., Duncan, M.E., O’Neil, P., Melvin, W.T. and Fothergill, J.E. (1996) Matrix Metalloproteinase-1 Is Associated with Poor Prognosis in Colorectal Cancer. Nature Medicine, 2, 461-462.
http://dx.doi.org/10.1038/nm0496-461

[12] Shiozawa, J., Ito, M., Nakayama, T., Nakashima, M., Kohno, S. and Sekine, I. (2000) Expression of Matrix Metalloproteinase-1 in Human Colorectal Carcinoma. Modern Pathology, 13, 925-933.
http://dx.doi.org/10.1038/modpathol.3880169

[13] Poola, I., DeWitty, R.L., Marshalleck, J.J., Bhatnagar, R., Abraham, J. and Leffall, L.D. (2005) Identification of MMP-1 as a Putative Breast Cancer Predictive Marker by Global Gene Expression Analysis. Nature Medicine, 11, 481-483.
http://dx.doi.org/10.1038/nm1243

[14] Das, S., Banerji, A., Frei, E. and Chatterjee, A. (2008) Rapid Expression and Activation of MMP-2 and MMP-9 upon Exposure of Human Breast Cancer Cells (MCF-7) to Fibronectin in Serum Free Medium. Life Sciences, 82, 467-476.
http://dx.doi.org/10.1016/j.lfs.2007.12.013

[15] Maity, G., Choudhury, P.R., Sen, T., Ganguly, K.K., Sil, H. and Chatterjee, A. (2011) Culture of Human Breast Cancer Cell Line (MDA-MB-231) on Fibronectin-Coated Surface Induces Pro-Matrix Metalloproteinase-9 Expression and Activity. Tumor Biology, 32, 129-138.
http://dx.doi.org/10.1007/s13277-010-0106-9

[16] Maity, G., Sen, T. and Chatterjee, A. (2011) Laminin Induces Matrix Metalloproteinase-9 Expression and Activation in Human Cervical Cancer Cell Line (SiHa). Journal of Cancer Research and Clinical Oncology, 137, 347-357.
http://dx.doi.org/10.1007/s00432-010-0892-x

[17] Pal, S., Ganguly, K.K. and Chatterjee, A. (2013) Extracellular Matrix Protein Fibronectin Induces Matrix Metalloproteinases in Human Prostate Adenocarcinoma Cells PC-3. Cell Communication and Adhesion, 20, 105-114.
http://dx.doi.org/10.3109/15419061.2013.833193

[18] Pal, S., Moulik, S., Dutta, A. and Chatterjee, A. (2014) Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7. Cancer Microenvironment, 7, 71-78.
http://dx.doi.org/10.1007/s12307-014-0146-6

[19] Sen, T., Dutta, A., Maity, G. and Chatterjee, A. (2010) Fibronectin Induces Matrix Metalloproteinase-9 (MMP-9) in Human Laryngeal Carcinoma Cells by Involving Multiple Signaling Pathways. Biochimie, 92, 1422-1434.
http://dx.doi.org/10.1016/j.biochi.2010.07.005

[20] Pal, S., Ganguly, K.K., Moulik, S. and Chatterjee, A. (2012) Modulation of MMPs by Cell Surface Integrin Receptor α5β1. Anti-Cancer Agents in Medicinal Chemistry, 12, 726-732.
http://dx.doi.org/10.2174/187152012802650183

[21] Jia, Y., Zeng, Z.Z., Markwart, S.M., Rockwood, K.F., Ignatoski, K.M., Ethier, S.P. and Livant, D.L. (2004) Integrin Fibronectin Receptors in Matrix Metalloproteinase-1-Dependent Invasion by Breast Cancer and Mammary Epithelial Cells. Cancer Research, 64, 8674-8681.
http://dx.doi.org/10.1158/0008-5472.CAN-04-0069