O. G. Kulikova¹, D. I. Maltsev¹, A. P. Ilyina¹, V. P. Yamskova², I. A. Yamskov¹

Show more
¹ A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Moscow, Russia.
² Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.
Show less

Abstract: Peptides (molecular weights are 1200 – 11,600 Da) biologically acting at concentration 10^-8 - 10^-15 mg/ml were obtained from leaves of dill Anethum graveolens L. and celandine Chelidonium majus L. by circular dichroism (CD) spectroscopy. We have shown that these peptides form second structure characterized by the prevalence of β-structures and coil in water solution. In model of organotypic roller culturing of triton liver in vitro, the ability of the peptides isolated from Anethum graveolens L. and Chelidonium majus L. at concentration 10^-8 - 10^-15 mg/ml to influence increasing of pigment cells, which provided protective function of organism, was demonstrated.

Keywords: Peptides, Medical Plants, Organotypic Roller Culturing, Newt Liver

Cite this paper: Kulikova, O. , Maltsev, D. , Ilyina, A. , Yamskova, V. and Yamskov, I. (2015) Hepatoprotective Activity of Plant Peptides. American Journal of Plant Sciences, 6, 848-855. doi: 10.4236/ajps.2015.67092.

References

[1]   Olennikov, D.N., Tanshaeva, L.M. and Michailova, T.M. (2005) Organic Acid of Official Plants. Chemistry of Natural Compounds, 4, 354-355.

[2]   Masoomeh, S.G., Shokoohamiri, M.R., Amirrajab, N., Behnaz, M., Ali, G., Farideh, Z., Golnar, S. and Mehdi, R.A. (2006) In Vitro Antifungal Activities of Allium cepa, Allium sativum and ketoconazole against Some Pathogenic Yeasts and Dermatophytes. Fitoterapia, 77, 321-323.
http://dx.doi.org/10.1016/j.fitote.2006.03.014

[3]   Yamskova, V.P., Krasnov, M.S. and Yamskov, I.A. (2012) New Experimental and Theoretical Aspects of Bioregulation. Action Mechanism of Membranotropic Homeostatic Tissue-Specific Bioregulators. Lambert Academic Publishing, Saarbrucken, 136 р.

[4]   Borisenko, A.V., Yamskova, V.P., Krasnov, M.S., Blagodatskikh, I.V., Vecherkin, V.V. and Yamskov, I.A. (2007) Regulatory Proteins from the Mammalian Liver That Display Biological Activity at Ultra Low Doses. In: Varfolomeev, S.D., Burlakova, E.B., Popov, A.A. and Zaikov, G.E., Eds., Biochemical Physics Frontal Research, Nova Science Publishers Inc., Hauppauge, 35-46.

[5]   Margasyuk, D.V., Krasnov, M.S., Blagodatskikh, I.V., Grigoryan, E.N., Yamskova, V.P. and Yamskov, I.A. (2007) Regulatory Protein from Bovine Cornea: Localization and Biological Activity. In: Varfolomeev, S.D., Burlakova, E.B., Popov, A.A. and Zaikov, G.E., Eds., Biochemical Physics Frontal Research, Nova Science Publishers Inc., Hauppauge, 47-60.

[6]   Yamskova, V.P., Krasnov, M.S., Rybakova, E.Yu., Vecherkin, V.V., Borisenko, A.V. and Yamskov, I.A. (2007) Analysis of Regulatory Proteins from Bovine Blood Serum That Display Biological Activity at Ultra Low Doses: 2. Tissue Localization and Role in Wound Healing. In: Varfolomeev, S.D., Burlakova, E.B., Popov, A.A. and Zaikov, G.E., Eds., Biochemical Physics Frontal Research, Nova Science Publishers Inc., Hauppauge, 71-78.

[7]   Krasnov, M.S., Yamskova, V.P., Kulikova, O.G., Ilyina, A.P., Margasyuk, D.V., Rybakova, E.Yu. and Yamskov, I.A. (2011) Study of New Bioregulators Isolated from Plantago majus L. Applied Biochemistry and Microbiology, 47, 146-153.

[8]   Kulikova, O.G., Yamskova, V.P., Ilyina, A.P., Margasyuk, D.V., Molyavka, A.A. and Yamskov, I.A. (2011) Identification of a New Bioregulators Acting in Ultra-Low Doses in Bulb Onion. Applied Biochemistry and Microbiology, 47, 1-5.

[9]   Jana, S. and Shekhawat, G.S. (2010) Anethum graveolens: An Indian Traditional Medicinal Herb and Spice. Pharmacognosy Review, 4, 179-184.
http://dx.doi.org/10.4103/0973-7847.70915

[10]   Gilca, M., Gaman, L., Panait, E., Stoian, I. and Atanasiu, V. (2010) Chelidionium majus—An Integrative Review: Traditional Knowledge versus Modern Findings. Forschende Komplementarmedizin, 17, 241-248.
http://dx.doi.org/10.1159/000321397

[11]   Laemmli, U.K. (1970) Nature.

[12]   Malcev, D.I., Yamskova, V.P. and Yamskov, I.A. (2012) Tissue Specificity Study of Bioregulators Isolated from Liver and Lung of Mammals. Journal of Information, Intelligence and Knowledge, 4, 3-9.

[13]   Corsaro, C., Scalia, M., Leotta, N., Mondio, F. and Sichel, G. (2000) Characterization of Kupffer Cells in Some Amphibia. Journal of Anatomy, 196, 249-261.
http://dx.doi.org/10.1046/j.1469-7580.2000.19620249.x

[14]   Sichel, G., Scalia, M. and Corsaro, C. (2002) Amphibia Kupffer Cells. Microscopy Research and Technique, 57, 477- 490.
http://dx.doi.org/10.1002/jemt.10101