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 OJAS  Vol.5 No.2 , April 2015
Relationship of Naturally Occurring Antisperm Antibodies in Blood Serum and Seminal Plasma of Cattle Bulls with Sperm Function and Fertility Tests
Abstract: The study was planned with an objective to assess the level of antisperm antibodies (ASA) in the blood serum and seminal plasma of breeding cow bulls and their relationship with sperm function and fertility tests. ASA was analyzed in blood serum and seminal plasma by SpermMar test, Immuno peroxidase assay (IPA) and Enzyme linked immunoabsorbant assay (ELISA). In SpermMar test, about 54% bulls were with >40% IgG in blood serum against sperm surface antigens, whereas none of the bulls were with >10% IgG in seminal plasma. More than 20% and >10% IgA against sperm surface antigens were detected in the blood serum and seminal plasma of 65.8% and 37% bulls, respectively. Out of 26 bulls, seminal plasma of 21 bulls reacted with spermatozoa both in IPA and IgA latex particles and that of only 12 bulls reacted with IgG. In IPA, about 50% of the bulls had >40% ASA against head surface antigens, whereas, there were 23% bulls with >10% ASA in seminal plasma. Also ELISA indicated a higher antibody titre in blood serum (3200 - 6400) and seminal plasma (40 - 80) of 50% and 42% bulls, respectively. There were 11 bulls with low values of HOST/in vitro acrosome reaction/cervical mucus penetration assay and higher level of either serum or seminal plasma ASA. Our study revealed that a significant level of ASA in serum or seminal plasma may have effect on the fertility of bulls by affecting the sperm function.
Cite this paper: Zodinsanga, V. , Cheema, R. and Mavi, P. (2015) Relationship of Naturally Occurring Antisperm Antibodies in Blood Serum and Seminal Plasma of Cattle Bulls with Sperm Function and Fertility Tests. Open Journal of Animal Sciences, 5, 114-123. doi: 10.4236/ojas.2015.52014.
References

[1]   Zraly, Z., Bendova, J., Diblikova, I., Svecova, D., Kummer, V., Maskova, J. and Veznik, Z. (2002) Antisperm Antibodies in Blood Sera of Bulls and Correlations with Age, Breed and Ejaculate Quality. Acta Veterinaria, 71, 303-308.
http://dx.doi.org/10.2754/avb200271030303

[2]   Murdoch, A.J., Buckley, C.H. and Fox, H. (1982) Hormonal Control of the Secretory Immune System of the Human Uterine Cervix. Journal of Reproductive Immunology, 4, 23-30.
http://dx.doi.org/10.1016/0165-0378(82)90020-1

[3]   Bronson, R.A., Cooper, G.W., Rosenfeld, D.L. and Witkin, S.S. (1984) Detection of Spontaneously Occurring Sperm-Directed Antibodies in Infertile Couples by Immunobead Binding and Enzyme-Linked Immunosorbent Assay. Annals of New York Academy of Sciences, 438, 504-507.
http://dx.doi.org/10.1111/j.1749-6632.1984.tb38318.x

[4]   Kim, C.A., Parrish, J.J., Momont, H.W. and Lunn, D.P. (1999) Effects of Experimentally Generated Bull Antisperm Antibodies on in Vitro Fertilization. Biology of Reproduction, 60, 1285-1291.
http://dx.doi.org/10.1095/biolreprod60.6.1285

[5]   Lombardo, F., Gandini, L., Dondero, F. and Lenzi, A. (2001) Immunology and Immunopathology of the Male Genital Tract: Antisperm Immunity in Natural and Assisted Reproduction. Human Reproduction Update, 7, 450-456.
http://dx.doi.org/10.1093/humupd/7.5.450

[6]   Blom, E. (1950) A One-Minute Live-Dead Sperm Stain by Means of Eosin-Nigrosin. Fertility Sterility, 1, 176-177.

[7]   Correa, J.R. and Zavos, P.M. (1994) The Hypo-Osmotic Swelling Test: Its Employment as an Assay to Evaluate the Functional Integrity of the Frozen-Thawed Bovine Sperm Membrane. Theriogenology, 42, 351-360.
http://dx.doi.org/10.1016/0093-691X(94)90280-1

[8]   Murase, T. and Braun, J.W. (1990) Impact of Methodological Factors on Sperm Penetration into Cervical Mucus in Cattle. Theriogenology, 34, 73-80. http://dx.doi.org/10.1016/0093-691X(90)90578-H

[9]   Sarma, D.K., Baishya, N., Sharma, D.K., Deka, B.C. and Bhuyan, D. (2009) Antisperm Antibodies in Serum and Cervical Mucus of Normal and Repeat Breeding Cows. Indian Journal of Animal Reproduction, 30, 54-56.

[10]   Parrish, J.J., Susko-Parrish, J.L. and First, N.L. (1989) Capacitation of Bovine Sperm by Heparin: Inhibitory Effect of Glucose and Role of Intracellular pH. Biology of Reproduction, 41, 683-699.
http://dx.doi.org/10.1095/biolreprod41.4.683

[11]   Fayemi, O. (2005) Sperm Antibodies and Reproductive Efficiency in the Zebu Cattle in South Western Nigeria. Pakistan Veterinary Journal, 25, 111-114.

[12]   FertiPro, N.V. (2011) A Qualitative Latex Test for Detection of Sperm Antibodies. Bijsluiter Sperm Mar Indd, 12, 31- 32.

[13]   Crowther, J.R. (1995) Methods in Molecular Biology. In: Crowther, J.R., Ed., ELISA: Theory and Practice, Humana Press, Totowa, 415-420.

[14]   Comhaire, F.H., Mahmoud, A.M., Depuydt, C.E., Zalata, A.A. and Christophe, A.B. (1999) Mechanisms and Effects of Male Genital Tract Infection on Sperm Quality and Fertilizing Potential: The Andrologist’s Viewpoint. Human Reproduction Update, 5, 393-398.
http://dx.doi.org/10.1093/humupd/5.5.393

[15]   Milovanovic, A., Lazarevic, M., Milanovic, S., Kirovski, D. and Jovicin, M. (2005) Open Days Period and Antispermatozoal Antibodies in Artificially Inseminated Cows. Acta Veterinaria, 55, 449-460.
http://dx.doi.org/10.2298/AVB0506449M

[16]   Stedronska, J. and Hendry, W.F. (1983) The Value of the Mixed Antiglobulin Reaction (MAR-Test) as an Addition to Routine Seminal Analysis in the Evaluation of the Subfertile Couple. American Journal of Reproduction, Immunology and Microbiology, 3, 89-91.
http://dx.doi.org/10.1111/j.1600-0897.1983.tb00221.x

[17]   Kurpisz, M. and Alexander, N.J. (1995) Carbohydrate Moieties on Sperm Surface: Physiological Relevance. Fertility and Sterility, 63, 158-165.

[18]   Ackerman, S., McGuire, G., Fulgham, D.L. and Alexander, N. (1988) An Evaluation of a Commercially Available Assay for the Detection of Antisperm Antibodies. Fertility and Sterility, 49, 732-734.

[19]   Romano, R., Santucci, R., Marrone, V. and Francavilla, F. (1993) Effect of Ionophore Challenge on Hamster Egg Penetration and Acrosome Reaction of Antibody-Coated Human Sperm. American Journal of Reproduction Immunology and Microbiology, 29, 56-61.
http://dx.doi.org/10.1111/j.1600-0897.1993.tb00838.x

[20]   Munoz, M.G. and Witkin, S.S. (1995) Autoimmunity to Spermatozoa, Asymptomatic Chlamydia trachomatis Genital Tract Infection and Gamma Delta T Lymphocytes in Seminal Fluid from the Male Partners of Couples with Unexplained Infertility. Human Reproduction Update, 10, 1070-1074.

[21]   Witkin, S.S., Kligman, I. and Bongiovanni, A.M. (1995) Relationship between an Asymptomatic Male Genital Tract Exposure to Chlamydia trachomatis and an Autoimmune Response to Spermatozoa. Human Reproduction Update, 10, 2952-2955.

[22]   Nikolaeva, M.A., Kulakov, V.I., Ter Avanesov, G.V., Terekhina, L.N., Pschenichinikova, T.J. and Sukhikh, G.T. (1993) Detection of Antisperm Antibodies on the Surface of Living Spermatozoa Using Flow Cytometry: Preliminary Study. Fertility and Sterility, 59, 639-644.

[23]   Meinertz, H., Linnet, L., Wolf, H. and Hjort, T. (1991) Antisperm Antibodies on Epididymal Spermatozoa. American Journal of Reproductive Immunology, 25, 158-162.
http://dx.doi.org/10.1111/j.1600-0897.1991.tb01087.x

[24]   Jarora, V., Gandotra, V.K., Cheema, R.S., Bansal, A.K. and Dhindsa, S.S. (2014) Relationship of in Vitro Cervical Mucus Penetration Assay with IgA and IgG Type Antibodies in Cross-Bred Cows. Advances in Animal and Veterinary Sciences, 2, 606-611.
http://dx.doi.org/10.14737/journal.aavs/2014/2.11.606.611

[25]   Fijak, M. and Meinhardt, A. (2006) The Testis in Immune Privilege. Immunological Reviews, 213, 66-81.
http://dx.doi.org/10.1111/j.1600-065X.2006.00438.x

[26]   Hunter, A.G. (1989) Immunology and Fertility in the Bovine. Journal of Dairy Science, 72, 3353-3362.
http://dx.doi.org/10.3168/jds.S0022-0302(89)79498-4

[27]   Chamley, L.W. and Clarke, G.N. (2007) Antisperm Antibodies and Conception. Seminars in Immunopathology, 29, 169-184. http://dx.doi.org/10.1007/s00281-007-0075-2

 
 
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