AJPS  Vol.6 No.5 , March 2015
In Vitro Evaluation of Some Traits in Stevia rebaudiana (Bertoni) under Drought Stress and Their Relationship on Stevioside Content
Abstract: The experiment was conducted to evaluate the differences of plant growth as well as sweetener material of two types of Stevia rebaudiana under conditions of absence and presence of drought stress. In vitro, the experiment was conducted as drought traits using four levels of polyethylene glycol (0.0, 10,000, 20,000, 30,000 ppm). It was observed that survival % and No. of shoots should be considered as the important factors associated with stevioside content in stevia plant. According to path coefficient analysis and positive direct effect of studied traits, survival % recorded the highest value (0.5386), whereas No. of shoots recorded the highest value (– 0.8827) as negative direct effect in related to stevioside content. The results showed that all traits were affected, especially under 30,000 ppm level of polyethylene glycol. Generally, the most of studies traits exhibited a recorded clearly difference between the two types and used drought levels on stevia plants. Therefore, selection based on survival % would be more effective to improving stevioside content of stevia plants in drought stress conditions.
Cite this paper: Badran, A. , Abd Alhady, M. and Hassan, W. (2015) In Vitro Evaluation of Some Traits in Stevia rebaudiana (Bertoni) under Drought Stress and Their Relationship on Stevioside Content. American Journal of Plant Sciences, 6, 746-752. doi: 10.4236/ajps.2015.65080.

[1]   Clinton, C.S. (1982) Rebaudi’s stevia: Natural Non Caloric Sweeteners. California Agriculture, 36, 9-10.

[2]   Carneiro, J.W.P., Muniz, A.S. and Guedes, T.A. (1997) Greenhouse Bedding Plant Production of Stevia rebaudiana (Bertoni). Canadian Journal of Plant Science, 77, 473-474.

[3]   Goettemoeller, J. and Ching, A. (1999) Seed Germination in Stevia rebaudiana. In: Janick, J., Ed., Perspectives on New Crops and New Uses, ASHS Press, Alexandria, 510-511.

[4]   Taware, A.S., Harke, S.N., Mukadam, D.S., Chavan, A.M. and Taware, S.D. (2010) Effect of Different Extracts of Callus and Plantlets of Stevia rebaudiana (Bertoni) on Seed Germination of Some Agricultural Crops. African Journal of Biotechnology, 9, 6675-6683.

[5]   Yadav, A.K., Singh, S., Dayani, D. and Ahuja, P.S. (2011) A Review on the Improvement of Stevia rebaudiana (Bertoni). Canadian Journal of Plant Science, 91, 1-27.

[6]   Bajji, M., Lutts, S. and Kinet, J.M. (2000) Physiological Changes after Exposure to and Recovery from Polyethylene Glycol-Induced Water Deficit in Callus Culture Issued from Durum Wheat (Triticum durum) Cultivars Differing in Drought Resistance. Plant Physiology, 156, 75-83.

[7]   Larher, F., Leport, L., Petrivalsky, M. and Chappart, M. (1993) Effectors for the Osmoinduced Proline Response in Higher Plants. Plant Physiology and Biochemistry, 31, 911-922.

[8]   Kulkarni, M. and Deshpande, U. (2007) In Vitro Screening of Tomato Genotypes for Drought Resistance Using Polyethylene Glycol. African Journal of Biotechnology, 6, 691-696.

[9]   Zgallai, H., Steppe, K. and Lemeur, R. (2005) Photosynthetic, Physiological and Biochemical Responses of Tomato Plants to Polyethylene Glycol-Induced Water Deficit. Journal of Integrative Plant Biology, 47, 1470-1478.

[10]   Koornneef, M., Bentsink, L. and Hilhorst, H. (2002) Seed Dormancy and Germination. Current Opinion in Plant Biology, 5, 33-36.

[11]   Cony, M.A. and Trione, S.O. (1998) Inter and Intra-Specific Variability in Prosopis flexuosa and P. chilensis: Seed Germination under Salt and Moisture Stress. Journal of Arid Environments, 40, 307-317.

[12]   Punia, M.S. and Gill, H.S. (1994) Correlations and Path Coefficient Analysis for Seed Yield Traits in Sunflower (Helianthus annuus L.). Helia, 17, 7-11.

[13]   Yasin, A.B. and Singh, S. (2010) Correlation and Path Coefficient Analyses in Sunflower. Journal of Plant Breeding and Crop Science, 2, 129-133.

[14]   Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.

[15]   Abd Alhady, M.R. (2011) Micropropagation of Stevia rebaudiana Bertoni. A New Sweetening Crop in Egypt. Global Journal of Biotechnology & Biochemistry, 6, 178.

[16]   Nishiyama, P., Alvarez, M. and Vieira, L.G.E. (1992) Quantitative Analysis of Stevioside in the Leaves of Stevia rebaudiana by Near Infrared Reflectance Spectroscopy. Journal of the Science of Food and Agriculture, 59, 277-281.

[17]   Michigan State University (1991) MSTATC, a Software Program for Design, Management and Analysis of Agronomic Research Experiments. Michigan State University, East Lansing.

[18]   Gomez, K.A. and Gomez, A.A. (1984) Statistical Procedures for Agriculture Research. A Wiley-Inter-Science Publication, John Wiley and Sons, New York.

[19]   Singh, R.K. and Chaudhary, B.D. (1995) Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publishers, New Delhi, 215-218.

[20]   Dewey, J.R. and Lu, K.H. (1959) A Correlation and Path-Coefficient Analysis of Components of Crested Wheatgrass Seed Production. Agronomy Journal, 51, 515-518.

[21]   Bewley, J.D. and Black, M. (1994) Seeds: Physiology of Development and Germination. 2nd Edition, Plenum Press, New York, London.

[22]   Van den Berg, L. and Zeng, Y.J. (2006) Response of South African Indigenous Grass Species to Drought Stress Induced by Polyethylene Glycol (PEG) 6000. South African Journal of Botany, 72, 284-286.

[23]   Shokoofeh, H. and Ali, A.E. (2013) Influence of Exogenously Applied Paclobutrazol on Some Physiological Traits and Growth of Stevia rebaudiana under in Vitro Drought Stress. Biologia, 68, 414-420.

[24]   Guangxi, R. and Yan, S. (2012) The Effects of Drought Stress on the Photosynthetic Parameters and Dry Leaf Yield of Stevia rebaudian Bertoni. Advanced Materials Research, 518-523, 4786.

[25]   Gupta, P., Sharma. S. and Saxena, S. (2014) Effect of Salts (NaCl and Na2CO3) on Callus and Suspension Culture of Stevia rebaudiana for Steviol Glycoside Production. Applied Biochemistry and Biotechnology, 172, 2894-2906.

[26]   Djibril, S., Mohamed, O.K., Diaga, D., Diegane, D., Abaye, B.F., Maurice, S. and Alaine, B. (2005) Growth and Development of Date Palm (Phoeix doctylifera L.) Seedling under Drought and Salinity Stresses. African Journal of Biotechnology, 4, 968-972.

[27]   Singh, R.K. and Kakar, S.N. (1977) Control on Individual Trait Means during Index Selection. Proceedings of the 3rd Congress on SABRAO, Canberra, III Ed, 22-25.