FNS  Vol.6 No.4 , March 2015
Different Mechanisms of Seed Kernel Extract from Mangifera indica on the Growth of Two Colon Cancer Cell Lines
Abstract: Flesh extract of Mangifera indica possesses anti-proliferation effect on different types of cancer cells. However, the effect of its seed kernel extract (MSP) on the growth of human colorectal carcinoma cells (CRC) has not yet been evaluated. Phenolic species of MSP were extracted and measured by colorimetry. Two CRC cell lines (Colo 320DM and SW480) were treated with MSP and assessed for viability by trypan blue exclusion, for cell cycle distribution by flow cytometry, for apoptosis by annexin V labeling, for mitochondria potential by rhodamine 123 staining and for changes in the levels of proteins involved in cell cycle control or apoptosis by immunoblotting. MSP inhibited the proliferation (12.5 μg/mL - 50 μg/mL) of Colo 320DM and SW480. MSP inhibited proliferation by blocking cell cycle progression at G1 (SW480) or S (Colo 320DM) phase and inducing apoptotic death. Western blotting indicated that MSP-blocking cell cycle was associated with cyclin levels. MSP-treated Colo 320DM and SW480 also showed activation of caspase 8, 9 and 3. MSP induces cell cycle arrest and apoptotic death in two CRC cell lines. The results indicate that MSP is a potential novel chemoprevention and treatment agent for colorectal cancer.
Cite this paper: Wu, C. , Hsu, C. , Lin, C. , Lu, F. , Huang, C. , Lin, Y. and Chen, C. (2015) Different Mechanisms of Seed Kernel Extract from Mangifera indica on the Growth of Two Colon Cancer Cell Lines. Food and Nutrition Sciences, 6, 421-428. doi: 10.4236/fns.2015.64043.

[1]   Bullock, M.D., Bruce, A., Sreekumar, R., Curtis, N., Cheung, T., Reading, I., Primrose, J.N., Ottensmeier, C., Packham, G.K., Thomas, G. and Mirnezami, A.H. (2013) FOXO3 Expression during Colorectal Cancer Progression: Biomarker Potential Reflects a Tumour Suppressor Role. British Journal of Cancer, 109, 387-394.

[2]   Cicerale, S., Lucas, L.J. and Keast, R.S. (2012) Antimicrobial, Antioxidant and Anti-Inflammatory Phenolic Activities in Extra Virgin Olive Oil. Current Opinion in Biotechnology, 23, 129-135.

[3]   Zamora-Ros, R., Rothwell, J.A., Scalbert, A., Knaze, V., Romieu, I., Slimani, N., Fagherazzi, G., Perquier, F., Touillaud, M., Molina-Monte, E., Huerta, J.M., Barricarte, A., Amiano, P., Menendez, V., Tumino, R., de Magistris, M.S., Palli, D., Ricceri, F., Sieri, S., Crowe, F.L., Khaw, K.T., Wareham, N.J., Grote, V., Li, K., Boeing, H., Forster, J., Trichopoulou, A., Benetou, V., Tsiotas, K., Bueno-de-Mesquita, H.B., Ros, M., Peeters, P.H., Tjonneland, A., Halkjaer, J., Overvad, K., Ericson, U., Wallstrom, P., Johansson, I., Landberg, R., Weiderpass, E., Engeset, D., Skeie, G., Wark, P., Riboli, E. and Gonzalez, C.A. (2013) Dietary Intakes and Food Sources of Phenolic Acids in the European Prospective Investigation Into Cancer and Nutrition (EPIC) study. British Journal of Nutrition, 110, 1500-1511.

[4]   Habib, H.M., Platat, C., Meudec, E., Cheynier, V. and Ibrahim, W.H. (2014) Polyphenolic Compounds in Date Fruit Seed (Phoenix dactylifera): Characterisation and Quantification by Using UPLC-DAD-ESI-MS. Journal of the Science of Food and Agriculture, 94, 1084-1089.

[5]   Rajan, S., Suganya, H., Thirunalasundari, T. and Jeeva, S. (2012) Antidiarrhoeal Efficacy of Mangifera indica Seed Kernel on Swiss Albino Mice. Asian Pacific Journal of Tropical Medicine, 5, 630-633.

[6]   Dorta, E., González, M., Lobo, M.G., Sánchez-Moreno, C. and De Ancos, B. (2014) Screening of Phenolic Compounds in By-Product Extracts from Mangoes (Mangifera indica L.) by HPLC-ESI-QTOF-MS and Multivariate Analysis for Use as a Food Ingredient. Food Research International, 57, 51-60.

[7]   Percival, S.S., Talcott, S.T., Chin, S.T., Mallak, A.C., Lounds-Singleton, A. and Pettit-Moore, J. (2006) Neoplastic Transformation of BALB/3T3 Cells and Cell Cycle of HL-60 Cells Are Inhibited by Mango (Mangifera indica L.) Juice and Mango Juice Extracts. Journal of Nutrition, 136, 1300-1304.

[8]   Prasad, S., Kalra, N. and Shukla, Y. (2007) Hepatoprotective Effects of Lupeol and Mango Pulp Extract of Carcinogen Induced Alteration in Swiss Albino Mice. Molecular Nutrition & Food Research, 51, 352-359.

[9]   Khan, N., Adhami, V.M. and Mukhtar, H. (2010) Apoptosis by Dietary Agents for Prevention and Treatment of Prostate Cancer. Endocrine-Related Cancers, 17, R39-R52.

[10]   Rajendran, P., Jayakumar, T., Nishigaki, I., Ekambaram, G., Nishigaki, Y., Vetriselvi, J. and Sakthisekaran, D. (2013) Immunomodulatory Effect of Mangiferin in Experimental Animals with Benzo(a)Pyrene-Induced Lung Carcinogenesis. International Journal of Biomedical Science, 9, 68-74.

[11]   Yao, Y.B., Peng, Z.G., Liu, Z.F., Yang, J. and Luo, J. (2010) Effects of Mangiferin on Cell Cycle Status and CDC2/ Cyclin B1 Expression of HL-60 Cells. Journal of Chinese Medicinal Materials, 33, 81-85.

[12]   Garcia-Rivera, D., Delgado, R., Bougarne, N., Haegeman, G. and Berghe, W.V. (2011) Gallic Acid Indanone and Mangiferin Xanthone Are Strong Determinants of Immunosuppressive Anti-Tumour Effects of Mangifera indica L. Bark in MDA-MB231 Breast Cancer Cells. Cancer Letters, 305, 21-31.

[13]   Noratto, G.D., Bertoldi, M.C., Krenek, K., Talcott, S.T., Stringheta, P.C. and Mertens-Talcott, S.U. (2010) Anticarcinogenic Effects of Polyphenolics from Mango (Mangifera indica) Varieties. Journal of Agricultural and Food Chemistry, 58, 4104-4112.

[14]   Abu Bakar, M.F., Mohamad, M., Rahmat, A., Burr, S.A. and Fry, J.R. (2010) Cytotoxicity, Cell Cycle Arrest, and Apoptosis in Breast Cancer Cell Lines Exposed to an Extract of the Seed Kernel of Mangifera pajang (Bambangan). Food and Chemical Toxicology, 48, 1688-1697.

[15]   Hsu, C.P., Lin, C.C., Huang, C.C., Lin, Y.H., Chou, J.C., Tsia, Y.T., Su, J. and Chung, Y.C. (2012) Induction of Apoptosis and Cell Cycle Arrest in Human Colorectal Carcinoma by Litchi Seed Extract. Journal of Biomedicine and Biotechnology, 2012, Article ID: 341479.

[16]   Fabisiak, J.P., Borisenko, G.G. and Kagan, V.E. (2014) Quantitative Method of Measuring Phosphatidylserine Externalization during Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron. Methods in Molecular Biology, 1105, 613-621.

[17]   Hsu, C.P., Lin, Y.H., Zhou, S.P., Chung, Y.C., Lin, C.C. and Wang, S.C. (2010) Longan Flower Extract Inhibits the Growth of Colorectal Carcinoma. Nutrition and Cancer, 62, 229-236.

[18]   Kundu, J.K. and Chun, K.S. (2014) The Promise of Dried Fruits in Cancer Chemoprevention. Asian Pacific Journal of Cancer Prevention, 15, 3343-3352.

[19]   Araujo, J.R., Goncalves, P. and Martel, F. (2011) Chemopreventive Effect of Dietary Polyphenols in Colorectal Cancer Cell Lines. Nutrition Research, 31, 77-87.

[20]   Guarnieri, S., Pilla, R., Morabito, C., Sacchetti, S., Mancinelli, R., Fano, G. and Mariggio, M.A. (2009) Extracellular Guanosine and GTP Promote Expression of Differentiation Markers and Induce S-Phase Cell-Cycle Arrest in Human SH-SY5Y Neuroblastoma Cells. International Journal of Developmental Neuroscience, 27, 135-147.

[21]   Jirawatnotai, S., Hu, Y., Livingston, D.M. and Sicinski, P. (2012) Proteomic Identification of a Direct Role for Cyclin D1 in DNA Damage Repair. Cancer Research, 72, 4289-4293.

[22]   Stratton, S.A. and Barton, M.C. (2014) Hierarchy of a Regenerative Cell Cycle: Cyclin E1 Multitasks. Hepatology, 59, 370-371.

[23]   Oh, S.M., Kim, J., Lee, J., Yi, J.M., Oh, D.S., Bang, O.S. and Kim, N.S. (2013) Anticancer Potential of an Ethanol Extract of Asiasari Radix against HCT-116 Human Colon Cancer Cells in Vitro. Oncology Letters, 5, 305-310.

[24]   Arcaroli, J.J., Quackenbush, K.S., Purkey, A., Powell, R.W., Pitts, T.M., Bagby, S., Tan, A.C., Cross, B., McPhillips, K., Song, E.K., Tai, W.M., Winn, R.A., Bikkavilli, K., Vanscoyk, M., Eckhardt, S.G. and Messersmith, W.A. (2013) Tumours with Elevated Levels of the Notch and Wnt Pathways Exhibit Efficacy to PF-03084014, a Gamma-Secretase Inhibitor, in a Preclinical Colorectal Explant Model. British Journal of Cancer, 109, 667-675.

[25]   Simpson, K.L., Cawthorne, C., Zhou, C., Hodgkinson, C.L., Walker, M.J., Trapani, F., Kadirvel, M., Brown, G., Dawson, M.J., MacFarlane, M., Williams, K.J., Whetton, A.D. and Dive, C. (2013) A Caspase-3 “Death-Switch” in Colorectal Cancer Cells for Induced and Synchronous Tumor Apoptosis in Vitro and in Vivo Facilitates the Development of Minimally Invasive Cell Death Biomarkers. Cell Death and Disease, 4, e613.

[26]   Galluzzi, L., Kepp, O., Trojel-Hansen, C. and Kroemer, G. (2012) Mitochondrial Control of Cellular Life, Stress, and Death. Circulation Research, 111, 1198-1207.