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 AJPS  Vol.6 No.5 , March 2015
Potential of Plant Growth Regulators on Modulating Rooting of Rosa centifolia
Abstract: Rosa centifolia is grown for its flowers which are used for essential oil extraction. It is vegetatively propagated through its cuttings which are difficult to root. This study was designed to increase the rooting percentage of cuttings by applying plant growth regulators (PGRs) with different levels. In total 31 treatments with different concentrations (450 ppm, 700 ppm and 950 ppm) of indole butyric acid (IBA), indole acetic acid (IAA), naphthalene acetic acid (NAA) alone, in combination and with same concentrations of 6-benzylamino purine (BAP) were used. Healthy similar sized cutting of R. centifolia was treated in solution of PGRs by quick dip method and planted in polythene bags. The data for shoot length, shoot dry weight, number of roots, root length and root dry weight were recorded. All three levels (450 ppm, 700 ppm and 950 ppm) of IBA alone produced maximum results in case of all parameters as compared to all other treatments. Among three levels of IBA, 450 ppm concentration produced maximum shoot length (10.67 cm), shoot dry weight (3.02 g), number of roots (14.00), root length (11.90 cm) and root dry weight (0.50 g). Lower concentrations of plant growth regulators produced better results as compared to higher concentrations.
Cite this paper: Akhtar, G. , Akram, A. , Sajjad, Y. , Balal, R. , Shahid, M. , Sardar, H. , Naseem, K. and Shah, S. (2015) Potential of Plant Growth Regulators on Modulating Rooting of Rosa centifolia. American Journal of Plant Sciences, 6, 659-665. doi: 10.4236/ajps.2015.65071.
References

[1]   Senapati, S.K. and Rout, G.R. (2008) Study of Culture Conditions for Improved Micropropagation of Hybrid Rose. Horticultural Science, 35, 27-34.

[2]   Gudin, S. (2000) Rose: Genetics and Breeding. Plant Breeding, 171, 59-89.

[3]   Lawrence, B.M. (1991) Progress in Essential Oil: Rose Oil and Extracts. Perfumer Flavorist, 16, 43-77.

[4]   Baydar, G.N., Baydar, H. and Debener, T. (2004) Analysis of Genetic Relationships among Rosa damascene Plants Grown in Turkey by Using AFLP and Microsatellite Markers. Journal of Biotechnology, 111, 263-267.
http://dx.doi.org/10.1016/j.jbiotec.2004.04.014

[5]   Nasir, M.H., Nadeem, R., Akhtar, K., Hanif, M.A. and Khalid, A.M. (2007) Efficacy of Modified Distillation Sludge of Rose (Rosa centifolia) Petals for Lead (II) and Zinc (II) Removal from Aqueous Solutions. Journal of Hazardous Materials, 147, 1006-1014.
http://dx.doi.org/10.1016/j.jhazmat.2007.01.131

[6]   Baydar, H. and Baydar, N.G. (2005) The Effects of Harvest Date, Fermentation Duration and Tween 20 Treatment on Essential Oil Content and Composition of Industrial Oil Rose (Rosa damascene Mill.). Industrial Crops and Products, 21, 251-255.
http://dx.doi.org/10.1016/j.indcrop.2004.04.004

[7]   Bayrak, A. and Akgul, A. (1994) Volatile Oil Composition of Turkish Rose (Rosa damascena). Journal of the Science of Food and Agriculture, 64, 441-448.
http://dx.doi.org/10.1002/jsfa.2740640408

[8]   Younis, A., Riaz, A., Khan, M.A. and Khan, A.A. (2009) Effect of Time of Growing Season and Time of Day for Flower Harvest on Flower Yield and Essential Oil Quality and Quantity of Four Rosa Cultivars. Floriculture Ornamental Biotechnology, 3, 98-103.

[9]   Simandi, B., Deak, A., Ronyai, E., Yanxiang, G., Veress, T., Lemberkovics, E., Then, M., Sasskiss, A. and Vamos-Falusi, Z. (1999) Supercritical Carbon Dioxide Extraction and Fractionation of Fennel Oil. Journal of Agricultural and Food Chemistry, 47, 1635-1640.
http://dx.doi.org/10.1021/jf9809535

[10]   Zieslin, N. (1996) Influence of Climatic and Socio-Economical Factors on Mode of Cultivation and Research of Rose Plants. Acta Horticulturae, 424, 21-22.

[11]   Horn, W.A.H., Schlegel, G. and Lerstuhl, K.J. (1992) Micropropagation of Roses (Rosa hybrida). Acta Horticulturae, 226, 623-627.

[12]   Rogers, R.B. and Smith, M.A.L. (1992) Consequence of in Vitro and ex Vitro Root Initiation for Miniature Rose Production. Journal of Horticultural Science, 67, 535-540.

[13]   Whitcomb, C.E. (1978) Propagating Woody Plants from Cuttings. Oklahoma State University Bulletin, 733, 13-15.

[14]   Husen, A. and Pal, M. (2003) Effect of Serial Bud Grafting and Etiolation on Rejuvenation and Rooting Cuttings of Mature Trees of Tectona grandis Linn. f. Silvae Genetica, 52, 84-88.

[15]   Cunningham, M.W. (1986) Evaluation of the Potential of Clonal Forestry for a Population of American Sycamore. North Carolina State University, Raleigh, 89.

[16]   Haines, P.I., Wong, C.Y. and Chia, E. (1992) Prospects for the Production of Superior Selection Age Phenotypes of Acacia mangium and A. auriculiformis. In: Breeding Technologies for Tropical Acacias, ACIAR Proceedings, No. 37, 11-118.

[17]   Rana, R.S. and Sood, K.K. (2012) Effect of Cutting Diameter and Hormonal Application on the Propagation of Ficus roxburghii Wall. through Branch Cuttings. Annals of Forest Research, 55, 69-84.

[18]   Sadhu, M.K. (1996) Plant Propagation. New Age International Publishers, New Delhi.

[19]   Grzesik, M. (1989) Effect of Growth Regulators on Growth and the Branching of Roses “Sonia” and “Mercedes”. Acta Horticulturae, 251, 411-414.

[20]   Leonard, R.T. and Nell, T.A. (2004) Short-Term Pulsing Improves Postharvest Leaf Quality of Cut Oriental Lilies. HortTechnology, 14, 405-411.

[21]   Evans, D.A., Sharp, W.R. and Flick, C.E. (1981) Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis. In: Thorpe, T.A., Ed., Plant Cell Culture: Methods and Applications in Agriculture, Academic Press, New York, 45-113.

[22]   Akinyele, A.O. (2010) Effects of Growth Hormones, Rooting Media and Leaf Size on Juvenile Stem Cuttings of Buchholzia coriacea Engler. Annals of Forest Research, 53, 127-123.

[23]   Raju, N.L. and Prasad, M.N.V. (2010) Influence of Growth Hormones on Adventitious Root Formation in Semi-Hardwood Cuttings of Celasturs paniculatus Willd.: A Contribution for Rapid Multiplication and Conservation Management. Agroforestry Systems, 79, 249-252.
http://dx.doi.org/10.1007/s10457-009-9251-9

[24]   Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. (2002) Plant Propagation, Principles and Practices. 6th Edition, Prentice Hall of India Pvt. Ltd., New Delhi.

[25]   Blazich, F.A. (1987) Using Calendar Dates for Cutting Propagation. American Nurseryman, 165, 140-143.

[26]   Oni, O. (1987) Effect of Auxins on the Rooting of Stem Cuttings of Terminalia superba (Engl. and Diels). In: Oguntala, A.B., Ed., The Role of Forestry in a Depressed Economy, Proceedings of the 17th Annual Conference of the Forestry Association of Nigeria, Ikeja, 6-10 December 1987, 43-48.

[27]   Ruppert, D.C. (1974) Hormone Concentrations. Combined Proceedings International Plant Propagators’ Society, 24, 349-350.

[28]   Chalapathi, M.V., Thimmegowda, N.D., Chandraprakash, J. and Gangadhar, G.E. (1999) Vegetative Propagation of Stevia (Stevia rebaudiana Bertoni) under Field Conditions. Crop Research, 18, 319-320.

[29]   Sulusoglu, M. and Cavusoglu, A. (2010) Vegetative Propagation of Cherry laurel (Prunus laurocerasus L.) Using Semi-Hardwood Cuttings. African Journal of Agricultural Research, 5, 3196-3202.

[30]   De Klerk, G. (2002) Rooting of Microcuttings: Theory and Practice. In Vitro Cellular & Developmental Biology-Plant, 38, 415-422.

[31]   Carboni, E., Tonelli, M.G., Lauri, P., Lacovacci, P., Kervers, C., Damiano, C. and Gaspar, T. (1997) Biochemical Aspects of Almond Micro-Cuttings Related to in Vitro Rooting and Their Requirements. Plant Physiology and Biochemistry, 28, 631-638.

[32]   Taiz, L. and Zeiger, E. (2006) Auxin: The Growth Hormone. In: Plant Physiology, 4th Edition, Sinauer Associates, Inc., Sunderland, 467-504.

[33]   George, E.F., Michael, A.H. and Geert, D.K. (2008) Plant Growth Regulators 1. Introduction, Auxins, Their Analogues and Antagonists. In: Plant Propagation by Tissue Culture, 3rd Edition, 175-197.

[34]   Han, H., Zhang, S. and Sun, X. (2009) A Review on the Molecular Mechanism of Plant Rooting Modulated by Auxin. African Journal of Biotechnology, 8, 348-353.

[35]   Husen, A. (2004) Clonal Propagation of Dalbergia sissoo Roxb. by Softwood Nodal Cuttings: Effects of Genotypes, Application of IBA and Position of Cuttings on Shoots. Silvae Genetica, 53, 50-55.

[36]   Chandramouli, H. (2001) Influence of Growth Regulators on the Rooting of Different Types of Cuttings in Burserapenicillata (DC) Engl. M.Sc. (Agri.) Thesis, University of Agricultural Sciences, Bangalore.

[37]   Carvalho, M.D., Zaidan, L. and De, C.M. (1995) Propagation of Stevia Rebaudiana from Stem Cuttings. Pesquisa Agropecuaria Brasileira, 30, 201-206.

[38]   Bhatt, B.B. and Tomar, Y.K. (2010) Effects of IBA on Rooting Performance of Citrus auriantifolia Swingle (Kagzi-Lime) in Different Growing Conditions. Nature and Science, 8, 8-11.

[39]   Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. (2002) Plant Propagation: Principles and Practices. 7th Edition, Prentice-Hall, Englewood Cliffs.

[40]   Iqbal, M., Khan, J.M.M., Fatima, B., Asif, M. and Abbas, M. (2003) In Vitro Propagation of Hybrid Tea Roses. Pakistan Journal of Agricultural Sciences, 40, 155-163.

[41]   Husen, A. and Pal, M. (2007) Metabolic Changes during Adventitious Root Primordium Development in Tectona grandis Linn. f. (Teak) Cuttings as Affected by Age of Donor Plants and Auxin (IBA and NAA) Treatment. New Forests, 33, 309-323.
http://dx.doi.org/10.1007/s11056-006-9030-7

[42]   Singh, A.K., Rajesh, S., Mittal, A.K., Singh, Y.P. and Shiva, J. (2003) Effect of Plant Growth Regulators on Survival Rooting and Growth Characters in Long Pepper (Piper longum L.). Progressive Horticulture, 35, 208-211.

[43]   Bagoury, E.L., Olfat, H., Allam, A.I., Abdelghany, A.M. and Attia, A.E. (2006) IBA Application to Stevia Stem Cutting: Its Productivity and Quality of Plant and Ratoon Crops. Egyptian Journal of Agricultural Research, 84, 299.

[44]   Dirr, M.A. (1981) Rooting Compounds and Their Use in Plant Propagation. Combined Proceedings International Plant Propagators’ Society, 31, 472-478.

[45]   Panjaitan, S.B., Aziz, M.A., Rashid, A.A. and Saleh, N.M. (2007) In-Vitro Plantlet Regeneration from Shoot Tip of Field-Grown Hermaphrodite Papaya (Carica papaya L. cv. Eksotika). International Journal of Agriculture & Biology, 9, 827-832.

[46]   Baker, C.M. and Wetzstein, H.Y. (2004) Influence of Auxin Type and Concentration on Peanut Somatic Embryogenesis. Plant Cell, Tissue and Organ Culture, 36, 361-368.
http://dx.doi.org/10.1007/BF00046094

[47]   Haq, I.U., Ahmad, T., Hafiz, I.A. and Abbasi, N.A. (2009) Influence of Microcutting Sizes and IBA Concentrations on in Vitro Rooting of Olive cv. “Dolce Agogia”. Pakistan Journal of Botany, 41, 1213-1222.

[48]   Shin, H.J. and Lee, J.M. (1979) Effect of Propagation Method and Growth Regulators on the Rooting of Chrysanthemum Cuttings. Journal of the Korean Society for Horticultural Science, 20, 319-327.

[49]   Khangoli, S. (2001) Potential of Growth Regulators on Control of Size and Flowering of Ornamental Plants. Proceedings of First Applied Scientific Seminar on Flowers and Ornamental Plants, Mahallat.

 
 
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